The separation of triacylglycerols using unpolar and medium polar capillary columns
M. Zárubová, V. Filip, J. Šmidrkal, T. Kůtek, I. Piskahttps://doi.org/10.17221/10633-CJFSCitation:M. Zárubová, V. Filip, J. Šmidrkal, T. Kůtek, I. Piska (2004): The separation of triacylglycerols using unpolar and medium polar capillary columns. Czech J. Food Sci., 22: 123-126.
Commercial by supplied triacylglycerols and synthesized triacylglycerols were used in this project. Analysed
triacylglycerols with the range of carbon number (CN) 24–54 consisted of saturated and unsaturated fay
acids. Two capillary columns were used in the analyses of triacylglycerols, namely non-polar (Optima®-1-TG) and
medium polar (Optima®-17-TG) types. In this study the effectiveness of separation (A) of triacylglycerols with
different carbon number values, (B) of triacylglycerols with different unsaturation degree, (C) of positional isomers
were determined and further possibilities to separate another lipid compounds were analysed. The column
Optima®-17-TG separates TAG according to the degree of unsaturation while the column Optima®-1-TG separates
saturated and unsaturated TAG. High temperature capillary gas chromatography was used to determine changes
in triacylglycerol composition during the transesterification of structured fats.
high temperature gas chromatography; triacylglycerol analysis