Antioxidative and antimicrobial effects of some natural extracts in lard Sekretár, Š. Schmidt, M. Vajdák, L. Zahradníková, J. Annus (2004): Antioxidative and antimicrobial effects of some natural extracts in lard. Czech J. Food Sci., 22: 215-218.
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The extracts from 17 ready available plants were prepared by 95% ethanol extraction in Soxhlet extractor.
The crude extracts were examined for their antioxidation properties using the automatized swift test (Rancimat)
and lard as a substrate. The extracts with the most promising results (Rosmarinus officinalis and Salvia officinalis)
were chromatographed on silica gel column and the fractions so obtained were explored separately. Second fraction
from the chromatography of rosemary extract exhibited the antioxidation activity (induction period 8 h at
0.002% wt.) comparable with the activity of butylated hydroxytoluene (BHT) at 12.5 times higher concentration
(induction period 11.45 h at 0.025% wt.). The antimicrobial efficiency of these extracts was determined with the aid
of three dimensional agar method on four selected microorganisms (Bacillus subtilis, Escherichia coli, Pseudomonas
aeruginosa, Staphylococcus aureus). The most effective were extracts from agrimony, black tea, rosemary and sage.
Extracts possessing good antioxidation activity also exhibited antimicrobial efficiency.

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