Chloropropanols and their esters in cereal products
C. G. Hamlet, P. A. Saddhttps://doi.org/10.17221/10676-CJFSCitation:C. G. Hamlet, P. A. Sadd (2004): Chloropropanols and their esters in cereal products . Czech J. Food Sci., 22: 259-262.
Chloropropanols, in particular monochloropropanediols (MCPDs) are food contaminants that can form
in the high temperature crust region of cereal products. Previous studies have indicated that MCPD-esters may
be formed from a reaction between chloride ions and some lipids, although, to date, these compounds have not
been reported in cereal products. MCPD-esters were extracted into an organic solvent and cleaned up by a preparative
thin-layer chromatography procedure. Sample extracts were analysed using gas chromatography/mass
spectrometry (GC/MS) and the data compared with that obtained from a reference MCPD-ester, prepared by a
custom synthesis. A faster method using enzyme hydrolysis and GC/MS showed that MCPD-esters could also be
determined as the amount of 3-MCPD released by a commercial lipase from Aspergillus oryzae. Under the conditions
of enzyme hydrolysis, model system studies indicated that low levels of MCPDs might also be generated
by a lipase catalysed reaction between short chain triacylglycerols and chloride ions.
cereal products; chloropropanol-esters; chloropropanols; lipase; 3-MCPD-esters