The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection
B. Mičková, P. Rauch, A. Montoya, E. Ferri, F. Fini, S. Girottihttps://doi.org/10.17221/10681-CJFSCitation:B. Mičková, P. Rauch, A. Montoya, E. Ferri, F. Fini, S. Girotti (2004): The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection. Czech J. Food Sci., 22: 280-282.
In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection
for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of
these assays were compared with those of ELISAs with colorimetric detection. The sensitivity of immunochemical
methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric
ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease
the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental
comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices,
spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs
were around 100% and no matrix effects were observed when fruit juices were diluted 1:20 or more.
N-methylcarbamate pesticides; chemiluminescent and colorimetric ELISA; fruit juices