I2-KI staining was used to phenotype 151 hulless barley plants, which determined that five samples were of the waxy variety, namely 14-Z152, IG107028, Puebla, Hu Zhu Shuang Cao Ren, and APM-HC1905. Using the dual-wavelength method, the average amylose content of the 151 samples was 25.9%, ranging from 4.9 to 38.5%. The average amylose content of the five waxy varieties was 14.3%, ranging from 4.9 to 18.6%. Genomic DNA from 48 samples showing a significantly variable amylose content was used as template and PCR amplified using primer pair P4. Statistical analysis indicated that the PCR product size positively correlated with amylose content. The Wx gene locus was determined to be polymorphic, and was positively correlated with amylose. Based on the electrophoresis results, the 48 samples were divided into 4 types. PCR product types I, II, III, and IV were 457, 481, 489, and 491 bp in length, respectively, with the respective amylose content ranges of 4.9–27%, 29–30%, 31–35%, and 36–38%. Primer P4 can be used as a complementary marker for the selection of different amylose content hulless barley germplasms.
Baik Byung-Kee, Ullrich Steven E. (2008): Barley for food: Characteristics, improvement, and renewed interest. Journal of Cereal Science, 48, 233-242 https://doi.org/10.1016/j.jcs.2008.02.002
J. Bao, H. Corke, M. Sun (2002): Microsatellites in starch-synthesizing genes in relation to starch physicochemical properties in waxy rice ( Oryza sativa L.). TAG Theoretical and Applied Genetics, 105, 898-905 https://doi.org/10.1007/s00122-002-1049-3
Bollieh C.N., Webb B.D. (1973): Inheritance of amylose in two hybrid populations of rice. Cereal Chemistry, 50: 631–636.
Briney A., Wilson R., Potter R.H., Barclay I., Crosbie G., Appels R., Jones M.G.K. (1998): A PCR-based marker for selection of starch and potential noodle quality in wheat. Molecular Breeding, 4: 427–433.https://doi.org/10.1023/A:1009664917998
Chao S., Sharp P. J., Worland A. J., Warham E. J., Koebner R. M. D., Gale M. D. (1989): RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theoretical and Applied Genetics, 78, 495-504 https://doi.org/10.1007/BF00290833
Clark Joanna R., Robertson Morag, Ainsworth Charles C. (1991): Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein. Plant Molecular Biology, 16, 1099-1101 https://doi.org/10.1007/BF00016086
DENVER K., BARBER L. M., BURTON R., HEDLEY C. L., HYLTON C. M., JOHNSON S., JONES D. A., MARSHALL J., SMITH A. M., TATGE H., TOMLINSON K., WANG T. L. (1995): The isolation and characterization of novel low-amylose mutants of Pisum sativum L.. Plant, Cell and Environment, 18, 1019-1026 https://doi.org/10.1111/j.1365-3040.1995.tb00612.x
Domon Eiji, Saito Akira, Takeda Kazuyoshi (): Comparison of the waxy locus sequence from a non-waxy strain and two waxy mutants of spontaneous and artificial origins in barley. Genes & Genetic Systems, 77, 351-359 https://doi.org/10.1266/ggs.77.351
Domon E., Yanagisawa T., Saito A., Takeda K. (2004): Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two-pair primers. Plant Breeding, 123, 225-228 https://doi.org/10.1111/j.1439-0523.2004.00970.x
Hylton ChristopherM., Denyer Kay, Keeling PeterL., Chang Ming-Tang, Smith AlisonM. (1996): The effect of waxy mutations on the granule-bound starch synthases of barley and maize endosperms. Planta, 198, - https://doi.org/10.1007/BF00206248
Juliano B.O., Pascual C.G. (1980): Quality characteristics of milled rice grown in different countries. IRRI Research Paper Series, 48: 1–5.
Kleinhofs A. (1997): Integrating barley RFLP and classical marker maps. Barley Genetics Newsletter, 27: 105–12.
Kramer Herbert H., Blander Barbara A. Swomley (1961): Orienting Linkage Maps on the Chromosomes of Barley1. Crop Science, 1, 339- https://doi.org/10.2135/cropsci1961.0011183X000100050012x
McKenzie K. S., Rutger J. N. (1983): Genetic Analysis of Amylose Content, Alkali Spreading Score, and Grain Dimensions in Rice1. Crop Science, 23, 306- https://doi.org/10.2135/cropsci1983.0011183X002300020031x
Nakamura T., Yamamori M., Hirano H., Hidaka S. (1993): Identification of three Wx proteins in wheat (Triticum aestivum L.). Biochemical Genetics, 31, 75-86 https://doi.org/10.1007/BF02399821
Nakao S. (1950): On waxy barleys in Japan. Seiken Jiho, 4: 111–113.
Ono T., Suzuki H. (1957): Endosperm characters in hybrids between barley varieties with starchy and waxy endosperms. Seiken Jiho, 8: 11–19.
Rosichan J., Nilan R.A., Arenaz P., Kleinhofs A. (1979): Intragenic recombination at the waxy locus in Hordeum vulgare. Barley Genetics Newsletter, 9: 79–85.
Sharp P.J., Chao S., Desai S., Gale M.D. (1989): The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theoretical and Applied Genetics, 78, - https://doi.org/10.1007/BF00265294
Shure M., Wessler S., Fedoroff N. (1983): Molecular identification and isolation of the Waxy locus in maize. Cell, 35, 225-233 https://doi.org/10.1016/0092-8674(83)90225-8
Sun Y.Y., Lü Y., Dong C.L., Wang P.R., Huang X.Q., Deng X.J. (2005): Progress in regulation of rice Wx gene expression. Hereditas, 27: 79–85.
TABATA Mamoru (1961): Studies of a Gametophyte Factor in Barley. The Japanese journal of genetics, 36, 157-167 https://doi.org/10.1266/jjg.36.157
Vrinten P., Nakamura T., Yamamori M. (1999): Molecular characterization of waxy mutations in wheat. Molecular and General Genetics MGG, 261, 463-471 https://doi.org/10.1007/s004380050989
Wang F., Zhao H., Wang Y., Wang X.Z. (2005): Relationship between fragment length polymorphism with waxy and amylase content in wheat. Journal of Plant Physiology and Molecular Biology, 31: 269–274.
Wang H.P., Tang C.H., Liu S.X., Zhang L.P., Lu C.F. (2007): Analysis of waxy proteins in Shanxi winter wheat cultivars using SDS-PAGE and molecular markers. Acta Agriculture Boreali Sinica, 22: 98–102.
Zhu C.M., Zhang J. (2010): Genetic diversity analysis of Waxy barley in China based on SSR markers. Journal of Plant Genetic Resources, 11: 564–572.