First characterisation of minor and neglected Vitis vinifera L. cultivars from Mount Etna F., Nicolosi E., La Malfa S., Cicala A., Gentile A. (2018): First characterisation of minor and neglected Vitis vinifera L. cultivars from Mount Etna. Hort. Sci. (Prague), 45: 37-46.
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Eight minor and neglected cultivars of Vitis vinifera L. were characterised according to their ampelographic and agronomic traits and discriminated by molecular analysis with SSR markers. These results are the first complete ampelographic description of these minor and neglected cultivars from the Mount Etna region. The results of this study reveal high morphological diversity of ancient grapevines growing in this region. SSR markers enabled us to discriminate the cultivars and revealed the genetic divergence between them and several autochthonous cultivars. Our efforts could contribute to a better knowledge of grape biodiversity based on morphological and molecular data and could be useful for the development of a reliable germplasm conservation strategy. 
Alleweldt G. (1997): Genetics of grapevine breeding. Progress in Botany, 58: 441–454.
Botstein D., White L.R., Sholnick M., David R.W. (1980): Construction of a genetic linkage mapin man using restriction fragment length polymorphism. American Journal of Human Genetics, 32: 314–331.
Bourquin J.-C., Sonko A., Otten L., Walter B. (1993): Restriction fragment length polymorphism and molecular taxonomy in Vitis vinifera L.. Theoretical and Applied Genetics, 87, -
Bowers J. E., Dangl G. S., Vignani R., Meredith C. P. (1996): Isolation and characterization of new polymorphic simple sequence repeat loci in grape ( Vitis vinifera L.). Genome, 39, 628-633
Bowers J.E., Dangl G.S., Meredith C.P. (1999): Development and characterization of additional microsatellite DNA markers for grape. American Journal of Enology and Viticulture, 50: 243–246.
Crespan M., Milani N. (2001): The Muscats: a molecular analysis of synonyms, homonyms and genetic relationship within a large family of grapevine cultivars. Vitis, 40: 23–30.
De Lorenzis Gabriella, Imazio Serena, Biagini Barbara, Failla Osvaldo, Scienza Attilio (2013): Pedigree Reconstruction of the Italian Grapevine Aglianico (Vitis vinifera L.) from Campania. Molecular Biotechnology, 54, 634-642
De Mattia F., Imazio S., Grassi F., Lovicu G., Tardaguila J., Failla O., Maitt C., Scienza A., Labra M (2007): Genetic characterization of sardina grapevine cultivars by SSR markers analysis. Journal International des Sciences de la Vigne et du Vin, 41: 175–184.
Ergul A., Marasal B., Agaoglu Y.S. (2002): Molecular discrimination and identification of some Turkish grape cultivars (Vitis vinifera L.) by RAPD markers. Vitis, 41: 159–160.
Fossati T., Labra M., Castiglione S., Failla O., Scienza A., Sala F. (2001): The use of AFLP and SSR molecular markers to decipher homonyms and synonyms in grapevine cultivars: the case of the varietal group known as ’’Schiave''. TAG Theoretical and Applied Genetics, 102, 200-205
Genres-081 (1997): Descriptors for grapevine (Vitis spp.) International Union for the Protection of New Varieties of Plants Geneva Switzerland/Office International de la Vigne et du Vin Paris France/International Plant Genetic Resources Institute, Rome, Italy.
Geremia G. (1834): Delle viti intorno all’Etna. Atti dell’Acca-demia Gioenia.
Liu K., Muse S. V. (2005): PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 21, 2128-2129
Meier U. (2001): Growth stages of mono-and dicotyledonous plants, BBCH Monograph, 2nd Ed. Federal Biological Research Centre for Agriculture and Forestry.
Molon G. (1906): Ampelografia. Vol. II, Hoepli, Milano.
Organisation Internationale de la Vigne et du Vin (OIV) (1983): Codes des caractères descriptifs des variétés et espèces de Vitis. A. Dedon, Paris.
Pàstena B. (1989): La civiltà della vite in Sicilia. Editrice Leopardi.
Perrier X., Jacquemoud-Collet J.P. (2006): DARwin software. Dissimilarity analysis and representation for windows. Available at
Rossoni M., Labra M., Imazio S., Grassi F., Scienza A., Sala F. (2003): Genetic relationships among grapevine cultivars grown in Oltrepò Pavese (Italy). Vitis, 42: 31–34.
Sabir A., Tangolar S., Buyukalaca S., Kafkas S. (2018): Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivars. Czech Journal of Genetics and Plant Breeding, 45, 160-168
Saitou N., Nei M. (1987): The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406–425.
Santiago J.L., Boso S., Gago P., Alonso-Villaverde V., Martínez M.C. (2007): Molecular and ampelographic characterisation of Vitis vinifera L. "Albariño", "Savagnin Blanc" and "Caíño Blanco" shows that they are different cultivars. Spanish Journal of Agricultural Research, 5, 333-
Sefc Kristina M, Regner Ferdinand, Turetschek Eva, Glössl Josef, Steinkellner Herta (1999): Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome, 42, 367-373
Sefc K. M., Lopes M. S., Lefort F., Botta R., Roubelakis-Angelakis K. A., Ibáñez J., Pejić I., Wagner H. W., Glössl J., Steinkellner H. (2000): Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars. TAG Theoretical and Applied Genetics, 100, 498-505
Thomas M.R., Scott N.S. (1993): Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). Theoretical and Applied Genetics, 86, -
Thomas M.R., Matsumoto S., Cain P., Scott N.S. (1993): Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theoretical and Applied Genetics, 86-86, -
Ye G.N., Soylemezoglu G., Weeden N.F., Reısch B.I. (1998): Analysis of the relationship between grapevine cultivars, sports and clones via DNA fingerprinting. Vitis, 37: 33–38.
Zulini L., Fabro E., Peterlunger E. (2005): Characterisation of the grapevine cultivar Picolit by means of morphological descriptors and molecular markers. Vitis, 44: 35–38.
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