Template-Type: ReDIF-Article 1.0 Author-Name: O. Kukačková Author-Name: L. Čurda Author-Name: J. Jindřich Title: Multivariate calibration of raw cow milk using NIR spectroscopy. Abstract: At present, mid-IR spectroscopy is the preferred rapid method for milk analysis. NIR spectrometers have some advantages in comparison with mid-IR. The purpose of this study was to see a possibility of determining the total solids, fat and protein in milk. The total number of preserved raw cow milk samples was 65. NIR spectra were recorded in duplicate on the NIRS 6500 instrument (NIRSystems, Inc.) using a fiber optic probe. The reference method for milk samples used for calibration model was determined by oven drying to measure the total solids, by Gerber method to measure fat content, and by Kjeldahl method to measure protein content. A commercial spectral analysis NIRS 3 software was used to process and develop chemometric models. The calibration was calculated using the modified partial least squares (MPLS) regression. The following statistical values (correlation coefficient R and standard error of prediction SEP) were obtained: R = 0.975 and SEP = 0.160% for milk total solids, R = 0.967 and SEP = 0.179% for milk fat content, R = 0.965 and SEP = 0.049 % for protein content. Using the NIR instrument the multivariate models suitable for rapid fundamental analysis of milk were developed. Keywords: near infrared spectroscopy, raw cow milk, total solids, fat, protein Journal: Czech Journal of Food Sciences Pages: 1-4 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0001_multivariate-calibration-of-raw-cow-milk-using-nir-spectroscopy.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8299-CJFS Template-Type: ReDIF-Article 1.0 Author-Name: L. Čurda Author-Name: J. Rudolfová Title: Changes of iodine content in milk of cows treated with Betadine Abstract: The purpose of this study was to assess the changes of iodine content in milk associated with the treatment of milch cows with the disinfecting preparation Betadine on basis of active iodine. Altogether 17 samples were taken from milch cows treated after milking by immersion of teats into Betadine solution; further 3 samples from cows after uterine irrigation. Ten check samples from milch cows without treatment were analyzed too. The content of iodine in milk was determined by the sensitive spectrophotometric method based on the Sandell-Kolthoff reaction brought to an end with addition of ammonium ferrous sulphate. Organic matter of milk was destroyed by wet acid mineralization. Average concentration and confidence interval of iodine content in check samples were 58 ± 15 µg/l. Milk from cows treated after milking with Betadine was not contaminated with iodine (53 ± 18 µg/l), increased variable concentration of iodine (from 283 to 1078 µg/l) was found in the milk from cows after uterine irrigation by Betadine Keywords: spectrophotometry, milk, iodine, disinfectant, Betadine, Sandell-Kolthoff reaction Journal: Czech Journal of Food Sciences Pages: 5-8 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0002_changes-of-iodine-content-in-milk-of-cows-treated-with-betadine.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8301-CJFS Template-Type: ReDIF-Article 1.0 Author-Name: P. Kadlec Author-Name: A. Rubecová Author-Name: Z. Réblová Author-Name: H. Štarhová Author-Name: Z. Bubník Title: Influence of germination on the carbohydrate composition of pea Abstract: Influence of germination on the carbohydrate composition of pea seeds was studied. After 4 days of germination the content of raffinose family oligosaccharides (RFO) in pea decreased to 17.5% of the original value and the ratio sucrose/RFO in cotyledons increased from 0.5 to 4.5. Keywords: pea seeds, germination, carbohydrates Journal: Czech Journal of Food Sciences Pages: 9-13 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0003_influence-of-germination-on-the-carbohydrate-composition-of-pea.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8300-CJFS Template-Type: ReDIF-Article 1.0 Author-Name: A. Hinková Author-Name: Z. Bubník Author-Name: H. Štarhová Author-Name: V. Pour Author-Name: P. Kadlec Title: Storage of sugar beet raw juice Abstract: Big disadvantage in sugar beet processing is the possibility of sugar beet treatment only during three month period. This paper deals with possibilities of raw juice long term storage without adding of any chemical additives. For this purpose sugar beet raw juice concentrates were prepared on the climbing film evaporator during two sugar campaigns 97/98 and 98/99. The final refractometric dry solid content varied from 40 to 72%. Some juices were pre-treated by micro- or ultrafiltration. Concentrates were stored for the period of 5 months (campaign 97/98) and 7 months (campaign 98/99) in a stock-room without any heating. Temperatures in the stock-room were very close to the outdoor average temperatures. It resulted from the analysis that initial dry solid content about 65% seemed to be sufficient for avoiding of microbiological decomposition and slight sucrose inversion could be expected but not any other monosaccharide decomposition. Initial dry solid content of juice concentrates at least 70 % assured high stability. There was not found any relevant influence of used methods for raw juice pre-treatment on concentrate durability. Keywords: sugar beet, raw juice storage, bioethanol, microfiltration, climbing film evaporator Journal: Czech Journal of Food Sciences Pages: 14-22 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0004_storage-of-sugar-beet-raw-juice.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8302-CJFS Template-Type: ReDIF-Article 1.0 Author-Name: Z. Bubník Author-Name: P. Kadlec Author-Name: H. Štarhová Author-Name: V. Pour Author-Name: A. Hinková Title: Application of computer image analysis for evaluation and control of the sucrose crystallization process Abstract: The paper deals with applications of computer image analysis to evaluation and control of crystallisation process in technical sugar solutions. Fields of application of this system: The kinetics study of the cooling crystallisation of sucrose from technical sugar solutions, measurement of the solubility of sucrose in technical sugar juice by the saturoscopy method, measurement of crystal size distribution. Keywords: computer image analysis, saturoscopic method, CSD, raw sugar juice Journal: Czech Journal of Food Sciences Pages: 23-28 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0005_application-of-computer-image-analysis-for-evaluation-and-control-of-the-sucrose-crystallization-process.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8303-CJFS Template-Type: ReDIF-Article 1.0 Author-Name: J. Čopíková Author-Name: A. Sinitsya Author-Name: K. Vaculová Title: Chromatography of barley b-glucans Abstract: Size exclusion chromatography with a set of two columns Separon Hema Bio 1000 and Separon Hema Bio 100 was found to give efficiency for the determination of molecular mass of ß-glucans isolated from barley. The set was calibrated with dextrans of known molecular mass. b-glucans from the barley cultivars ranged in molecular mass from 1.2 × 106 g/mol to 1.5 × 106 g/mol in the case of soluble b-glucans and from 0.9 × 106 g/mol to 1.4 × 106 g/mol in the case of total b-glucans. The new Czech varieties had higher values of molecular mass in comparison to parental genotypes. The differences between molecular mass of parental genotypes of high glucan barley and new varieties were statistically significant. Keywords: ß-glucans, barley, molecular mass, size-exclusion chromatography, biological value Journal: Czech Journal of Food Sciences Pages: 29-34 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0006_chromatography-of-barley-b-glucans.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8304-CJFS Template-Type: ReDIF-Article 1.0 Author-Name: K. Melzoch Author-Name: V. Filip Author-Name: D. Buckiová Author-Name: I. Hanzlíková Author-Name: J. Šmidrkal Title: Resveratrol - occurrence in wine originating from Czech vineyard regions and effect on human health Abstract: Resveratrol is a substance of a natural character, naturally present in some foods, namely in wine. The grape peels of Vitis vinifera contain about 50 to 100 mg of resveratrol/g and the average resveratrol concentration in red wines produced in the world fluctuates between 1.0 to 3.0 mg/l. A few biological activities were discovered with resveratrol in the last few years such as positive action against some cardiovascular diseases described as so called French paradox and anti-cancer chemo-preventive activity. The denominators of those activities are anti-oxidizing and free radical scavenging features of resveratrol molecule. As expected, red wines from vines originating in the Czech vineyard regions appear to contain relatively high levels of resveratrol (from 1.4 to 9.4 mg/l. Keywords: resveratrol, anti-oxidants, polyphenols, red wine, French paradox, anti-cancer chemo-preventive activity Journal: Czech Journal of Food Sciences Pages: 35-40 Volume: 18 Issue: 1 Year: 2000 File-URL: http://cjfs.agriculturejournals.cz/artkey/cjf-200001-0007_resveratrol-occurrence-in-wine-originating-from-czech-vineyard-regions-and-effect-on-human-health.php File-Format: text/html Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:1:id:8305-CJFS