Template-Type: ReDIF-Article 1.0 Author-Name: J. Salava Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: Y. Wang Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: B. Krška Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: J. Polák Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: P. Komínek Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: R.W. Miller Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: W.M. Dowler Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: G.L. Reighard Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Author-Name: A.G. Abbott Author-Workplace-Name: 3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA Title: Molecular genetic mapping in apricot Abstract: A genetic linkage map for apricot (Prunus armeniaca L.) has been constructed using amplified fragment length polymorphism (AFLP) markers in 80 BC1 individuals derived from a cross LE-3246 × Vestar. From 26 different primer combinations, a total of 248 AFLP markers were scored, of which, 40 were assigned to 8 linkage groups covering 315.8 cM of the apricot nuclear genome. The average interval between these markers was 7.7 cM. One gene (PPVres1) involved in resistance to PPV (Plum pox virus) was mapped. Two AFLP markers (EAA/MCAG8 and EAG/MCAT14) were found to be closely associated with the PPVres1 locus (4.6 cM resp. 4.7 cM). These markers are being characterized and they will be studied for utilization in apricot breeding with marker-assisted selection (MAS). Keywords: Prunus armeniaca L., Plum pox virus, resistance, AFLP, genetic mapping Journal: Czech Journal of Genetics and Plant Breeding Pages: 65-68 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6113-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6113-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200202-0001.txt Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6113-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: M. Baránek Author-Workplace-Name: Mendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic Author-Name: M. Kadlec Author-Workplace-Name: Mendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic Author-Name: J. Raddová Author-Workplace-Name: Mendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic Author-Name: M. Vachůn Author-Workplace-Name: Mendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic Author-Name: M. Pidra Author-Workplace-Name: Mendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic Title: Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes Abstract: The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process. Keywords: soybean, molecular genetics, genetic diversity, dendogram, RAPD Journal: Czech Journal of Genetics and Plant Breeding Pages: 69-74 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6114-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6114-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200202-0002.txt Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6114-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: S. Pažoutová Author-Workplace-Name: 2 Grassland Research Station, Zubří, Czech Republic Author-Name: B. Cagaš Author-Workplace-Name: 2 Grassland Research Station, Zubří, Czech Republic Author-Name: R. Kolínská Author-Workplace-Name: 2 Grassland Research Station, Zubří, Czech Republic Author-Name: A. Honzátko Author-Workplace-Name: 2 Grassland Research Station, Zubří, Czech Republic Title: Host specialization of different populations of ergot fungus (Claviceps purpurea) Abstract: In our previous study of Claviceps purpurea three populations were found: G1 on open localities, G2 from shady or wet habitats and G3 on Spartina stands of coastal salt marshes. The latter two are also chemoraces. In the Czech Republic, isolates of G1 and G2 were found. The ability of four isolates representing these populations to infect and develop sclerotia on different host species (Holcus lanatus, Helictotrichon pubescens, Phalaris arundinacea, Dactylis glomerata, Arrhenatherum elatius, Bromus inermis, Bromus erectus, Elytrigia repens, Avenella flexuosa, Lolium perenne, Poa nemoralis, Poa annua, and different cultivars of Poa pratensis) was studied along with their alkaloid production. P. pratensis and D. glomerata were infected by all the isolates and sclerotia were formed by isolates 207 (G1) and 434 (G2), and on two P. pratensis cultivars even by 481 (G3). Infection ability (formation of sphacelial stage and honeydew) was less host-restricted than formation of mature sclerotia. G2 and G3 strains infected A. flexuosa without sclerotia formation. L. perenne was infected only once by strain 207 (G1) without sclerotia formation. P. annua (natural host of G2), was infected by all isolates, but no sclerotia were formed even with G2 strains. From the two G2 isolates, strain 434 from Dactylis formed sclerotia on five host species, whereas isolate 475 originating from Phragmites stand formed only sphacelia. Composition of alkaloid mixture produced in sclerotia of the same strain from various hosts confirmed that host plant does not influence the type of alkaloids produced, only their ratio. Keywords: ergot, host specificity, alkaloids Journal: Czech Journal of Genetics and Plant Breeding Pages: 75-81 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6115-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6115-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200202-0003.txt Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6115-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: E. Schwarzbach Author-Workplace-Name: Miroslav, Czech Republic Title: The effects of silver on microbial contamination of agar medium and on interactions between mildew and barley leaf segments with and without the mlo gene Abstract: Segments of primary leaves of several barley varieties with and without the mlo gene were placed in Petri dishes on an agar medium containing benzimidazole, mineral nutrients and 0, 0.1, 0.3, 1 and 3 ppm of AgNO3. Three Petri dishes were prepared of each concentration. The segments were uniformly inoculated with 103 conidia/cm2 of the partially mlo-virulent powdery mildew culture PV-32. Subsequently, one open Petri dish of each Ag-concentration was exposed for 1 hour to a different potential con-tamination environment: one in the laboratory (low load), one in a humid cellar close to stored vegetables (medium load) and one on the top of a compost heap of decaying garbage (heavy load). Germination of mildew spores on the medium surface declined slightly with increasing concentration of AgNO3. Mildew infection was evaluated 7 days after inoculation. The number of mildew colonies per leaf segment and the differential interaction of the Mlo- and mlo-varieties with the mildew culture was apparently not affected by the AgNO3 concentration. Contamination of the medium by airborne micro-organisms was evaluated 12 days after exposure both microscopically and by eye. The contamination of the medium increased with environmental load and with decreasing AgNO3 concentration. 0.1 ppm AgNO3 markedly retarded the growth of contaminant colonies from all three environments, but did not prevent contamination. At 1 ppm AgNO3, no contamination was observed on the media exposed to low and medium load, but several dozen small contaminant colonies developed on the medium exposed to heavy load. At 3 ppm AgNO3, only three small contaminant colonies developed on the medium exposed to heavy load, while the media exposed to medium and low load remained clean. It can be concluded that adding 1 ppm AgNO3 to a mineral-agar medium efficiently suppresses its contamination under low and medium load,without apparently affecting the growth of mildew or the interaction between mildew and mlo-barley on leaf segments placed on the medium. Keywords: contamination, silver, AgNO3, barley, leaf segments, powdery mildew, mlo Journal: Czech Journal of Genetics and Plant Breeding Pages: 82-86 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6116-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6116-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200202-0004.txt Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6116-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: A. Hanišová Author-Name: P. Horčička Title: Winter wheat Svitava Journal: Czech Journal of Genetics and Plant Breeding Pages: 87-88 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6117-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6117-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6117-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: L. Bobková Title: Winter wheat Mladka Journal: Czech Journal of Genetics and Plant Breeding Pages: 88-89 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6118-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6118-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6118-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: L. Bobková Author-Name: V. Šíp Author-Name: M. Škorpík Title: Winter wheat Rheia Journal: Czech Journal of Genetics and Plant Breeding Pages: 90-91 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6119-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6119-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6119-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Čapek Title: Winter rye Aventino Journal: Czech Journal of Genetics and Plant Breeding Pages: 92-93 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6120-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6120-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6120-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Voral Title: Potato Komtesa Journal: Czech Journal of Genetics and Plant Breeding Pages: 94-95 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6121-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6121-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6121-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Bareš Title: Prof. Ing. Jaroslav Lekeš, DrSc. (1928-2002) Journal: Czech Journal of Genetics and Plant Breeding Pages: 96 Volume: 38 Issue: 2 Year: 2002 DOI: 10.17221/6122-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6122-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:38:y:2002:i:2:id:6122-CJGPB