Template-Type: ReDIF-Article 1.0 Author-Name: A. Lebeda Author-Workplace-Name: Deparment of Botany, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic Author-Name: I. Doležalová Author-Workplace-Name: Deparment of Botany, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic Author-Name: M. Dziechciarková Author-Workplace-Name: Deparment of Botany, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic Author-Name: K. Doležal Author-Workplace-Name: Deparment of Botany, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic Author-Name: J. Frček Author-Workplace-Name: Deparment of Botany, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic Title: Morphological Variability and Isozyme Polymorphisms in Maca and Yacon Abstract: A set of 15 accessions of maca, Lepidium meyenii Walp., and 25 accessions of yacon, Smallanthus sonchifolius (Poepp. & Endl.) H. Robins., cultivated under Czech field conditions was studied to determine relationships between morphological variability and isozyme polymorphisms. Morphological characterisation of maca included evaluation of length, weight, shape, skin and flesh colour of hypocotyls. In yacon, we evaluated shape, colour, skin texture and flesh colour of tubers, as well as the number of roots. Preliminary results showed that maca forms low-weight hypocotyls (up to only 17.4 g) under Czech field conditions. For yacon, tuber production ranged between 1.4 kg and 3.8 kg. Of the 17 analysed enzymatic systems, only esterase (EST) showed some degree of polymorphism in both crops. It was possible to divide accessions of L. meyenii into two groups and S. sonchifolius into six groups on the basis of isozyme polymorphisms. However, EST polymorphisms do not correspond very well to the morphological characteristics of the underground organs of the crops studied. Keywords: Andean crops, Lepidium meyenii, Smallanthus sonchifolius, functional food, morphological characters, tuber, hypocotyl, protein markers, enzyme Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-8 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/3714-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3714-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200301-0001.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:3714-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Gubiš Author-Workplace-Name: Slovak Republic Author-Name: Z. Lajchová Author-Workplace-Name: Slovak Republic Author-Name: J. Faragó Author-Workplace-Name: Slovak Republic Author-Name: Z. Jureková Author-Workplace-Name: Slovak Republic Title: Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro Abstract: The regeneration capacity of six types of explants (segments from hypocotyl, cotyledons, epicotyl, leaf, internodes and petiole) was compared in 13 cultivars of tomato (Lycopersicon esculentum Mill). Explants were cultured on a regeneration medium containing 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. The number of shoot primordia and shoots with 1 or more fully developed leaves was evaluated after 6 weeks. The regeneration capacity was significantly influenced by cultivars and explant types. The total number of shoot primordia produced in all types of explants was highest in the cultivars Hana and Premium and lowest in UC 82 and Money Marker. Cv. Hana also produced the highest number of shoots. The most responsive explants in most cultivars were hypocotyls and epicotyls with up to 100% regeneration and mean production of 6.3 and 6.5 shoot primordia per explant, respectively. Keywords: culture in vitro, organogenesis, Murashige-Skoog medium, indole-3-acetic acid, zeatin Journal: Czech Journal of Genetics and Plant Breeding Pages: 9-14 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/3715-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3715-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200301-0002.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:3715-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: T. Ledečan Author-Workplace-Name: Agricultural Institute Osijek, Department of Maize Breeding and Genetics, Osijek, Croatia Author-Name: D. Šumić Author-Workplace-Name: Agricultural Institute Osijek, Department of Maize Breeding and Genetics, Osijek, Croatia Author-Name: I. Brkić Author-Workplace-Name: Agricultural Institute Osijek, Department of Maize Breeding and Genetics, Osijek, Croatia Author-Name: A. Jambrović Author-Workplace-Name: Agricultural Institute Osijek, Department of Maize Breeding and Genetics, Osijek, Croatia Author-Name: Z. Zdunić Author-Workplace-Name: Agricultural Institute Osijek, Department of Maize Breeding and Genetics, Osijek, Croatia Title: Resistance of Maize Inbreds and their Hybrids to Fusarium Stalk Rot Abstract: The resistance of 35 inbred lines and their 70 hybrids with two testers to stalk rot is described and the results of natural and artificial stalk infection are compared. A mixture of Fusarium spp. spores was used for artificial infections. The disease was scored for two years. Inbreds and hybrids differed significantly in resistance and infection types in both years. Generally, disease scores of hybrids were lower than of inbreds. No close association was found between lines and their testcross hybrids (r = -0.06 to 0.29) and between hybrids with the two testers (r = -0.04 to 0.38). The correlation coefficients of disease scores after natural and artificial infection were in inbreds and in hybrids highly significant (r = 0.45-0.87). The results suggest, that Fusarium stalk rot resistance should be tested in both inbreds and hybrids. We recommend for maize breeding, that tests with artificial infection should be followed by tests under natural infection. Keywords: maize, Fusarium stalk rot, inbreds, hybrids, resistance of maize Journal: Czech Journal of Genetics and Plant Breeding Pages: 15-20 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/3716-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3716-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200301-0003.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:3716-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: L.G. Tyryshkin Author-Workplace-Name: N. I. Vavilov Institute of Plant Industry, St.-Petersburg, 190000 Russia Author-Name: N.A. Tyryshkina Author-Workplace-Name: N. I. Vavilov Institute of Plant Industry, St.-Petersburg, 190000 Russia Title: Resistance to Diseases in Wheat Collection Samples and Somaclonal Variants Abstract: More than 2000 wheat collection samples were studied for seedling resistance to 4 diseases. Noncoincidence of results obtained after inoculation of leaf segments placed on water solution of benzimidazole (60 ppm) and intact plants was found for 3 foliar diseases (leaf rust - Puccinia recondita, dark brown leaf spot blotch - Bipolaris sorokiniana and septoria glume blotch - Septoria nodorum). Sixty-three entries were resistant to leaf rust; 26 of them have gene for resistance Lr26 (non-effective under field condition), 25 - gene Lr24, 4 - gene Lr19, 8 - gene Lr9 and 2 - gene Lr41. Samples 181-5, Fielder, Butte 66, Raj 1972 and Soisson were moderately resistant to spot blotch; 181-5 - to common root rot and Moking and MN 81330 - to glume blotch. Very high level of partial resistance to 3 diseases was found in late generations of somaclonal lines of weakly and moderately resistant initial genotypes: to leaf rust in lines of cv. Spica, to leaf spot blotch - in lines of 181-5, to common root rot - in that of 181-5 and cv. Vera; moderate resistance to glume blotch was identified in lines of cv. Spica. Keywords: wheat, leaf rust, dark brown leaf spot blotch, common root rot, septoria glume blotch Journal: Czech Journal of Genetics and Plant Breeding Pages: 21-23 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/3717-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3717-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200301-0004.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:3717-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: M. Griga Title: The Czech-Slovak Workshop: Utilization of Molecular Markers in Plant Biology, Plant Breeding and Conservation of Plant Genetic Resources (Šumperk, 4th-6th November 2002) Journal: Czech Journal of Genetics and Plant Breeding Pages: 24 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/6096-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6096-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:6096-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: A. Hanišová Author-Name: P. Horčička Title: Spring wheat variety Zuzana Journal: Czech Journal of Genetics and Plant Breeding Pages: 25-26 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/6097-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6097-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:6097-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: M. Špunarová Title: Spring barley Respekt Journal: Czech Journal of Genetics and Plant Breeding Pages: 27-28 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/6098-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6098-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:6098-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Kreuzman Author-Name: R. Drdla Author-Name: P. Liška Title: Pea variety Herold Journal: Czech Journal of Genetics and Plant Breeding Pages: 29-30 Volume: 39 Issue: 1 Year: 2003 DOI: 10.17221/6099-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6099-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:1:id:6099-CJGPB