Template-Type: ReDIF-Article 1.0 Author-Name: Z. Stehno Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: L. Dotlačil Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: I. Faberová Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: S. Martynov Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: T. Dobrotvorskaya Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Genealogical Analysis of the Genetic Diversity in Winter Wheat Cultivars Grown in the Former Czechoslovakia and the Present Czech Republic during 1919-2001 Abstract: Using genealogical analysis, the genetic diversity in winter wheat cultivars registered and grown in the formerCzechoslovakia and the presentCzech Republic during 1919-2001 was studied. The strong increase of the diversity level since the 1970-s is based on the wide use in breeding programs of foreign materials, most of which originated in countries of Western, Central andEastern Europe. Simultaneously a genetic erosion in the released cultivars occurred; from the 1930-s to the 1970-s, a significant number of original local ancestors was lost. The modern cultivars listed in the Czech National List of Varieties in 2000-2001 can be distinguished into clusters. The overwhelming majority of cultivars belong to two clusters of similar extent. In one of them the ancestors fromWestern Europe can be found, while in the second cluster ancestors fromEastern Europe dominate, mainly through cvs. Mironovskaya 808 and Bezostaya 1. An index of similarity of modern cultivars grown in theCzech Republic is approximately equal to the average between half- and quarter-sibs. Consequently, it can be concluded that the genetic diversity in winter wheat cultivars presently grown in theCzech Republic has increased in the last decades and is considered as acceptable. Keywords: winter wheat, genetic profiles, original ancestors, genetic erosion, cluster analysis Journal: Czech Journal of Genetics and Plant Breeding Pages: 99-108 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3726-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3726-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200304-0001.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3726-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Ovesná Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: K. Poláková Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: L. Kučera Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: J. Rulcová Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model Abstract: We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Analyser. Keywords: SSCP, SNaPShot, Mlo, SNP, DNA marker, barley, primer extension Journal: Czech Journal of Genetics and Plant Breeding Pages: 109-112 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3727-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3727-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200304-0002.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3727-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Raddová Author-Workplace-Name: Mendeleum, Faculty of Horticulture, Lednice, Mendel University of Agriculture and Forestry in Brno, Czech Republic Author-Name: M. Beránek Author-Workplace-Name: Mendeleum, Faculty of Horticulture, Lednice, Mendel University of Agriculture and Forestry in Brno, Czech Republic Author-Name: I. Oukropec Author-Workplace-Name: Mendeleum, Faculty of Horticulture, Lednice, Mendel University of Agriculture and Forestry in Brno, Czech Republic Author-Name: M. Vachůn Author-Workplace-Name: Mendeleum, Faculty of Horticulture, Lednice, Mendel University of Agriculture and Forestry in Brno, Czech Republic Title: RAPD Analysis of peaches within Czech National collection Abstract: The Random Amplified Polymorphic DNA (RAPD) technique was used to study the genetic diversity and relationships within the collection of the Czech National Plant Genetic Resources (PGR) of peaches (Prunus persica L.). The aim of the work was to elaborate a dendrogram of genetic similarity and to divide collection into clusters. 46 primers were applied to 6 cultivars differing in the place of origin, the fruit shape, the fruit colour, and in some other morphological characteristics. 12 primers were chosen which gave polymorphic repeatable strong and middle strong bands. They were subsequently used for the RAPD reactions within the whole collection of peaches. The selected RAPD primers distinguished 28 peach cultivars and RAPD data were used to group the accessions analysed. Almonds and peach × almond hybrids were clearly separated in the frame of the whole collection. The grouping corresponded to the botanical system, to the available information about pedigree, and to the cultivars description. Keywords: Prunus persica, peach, RAPD primers, genetic resources Journal: Czech Journal of Genetics and Plant Breeding Pages: 113-119 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3728-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3728-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200304-0003.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3728-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: M. Hudcovicová Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: J. Kraic Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Title: Utilisation of SSRs for Characterisation of the Soybean (Glycine max (L.) Merr.) Genetic Resources Abstract: The SSR profiles of 67 soybean genotypes of various origins have been detected by 188 alleles at the 18 SSR loci. From 4 to 21 alleles were found at each of these loci (average 10.4 per locus) and the gene diversity averaged 0.71. Differentiation of all 67 genotypes each from others has been successful by using of even only 6 of SSR markers (Sat001, Satt005, Satt038, Satt173, Satt177, Satt534) with gene diversity from 0.66 to 0.89. The cumulated probability of obtaining identical soybean SSR profile was 1.11 × 10-9 , which confirms a high potential of SSRs for differentiation of soybean accessions in collections. Clustering of genotypes partially reflects origin and pedigree of analysed soybean accessions. Keywords: soybean, Glycine max (L.) Merr., microsatellite, genetic diversity Journal: Czech Journal of Genetics and Plant Breeding Pages: 120-126 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3729-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3729-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200304-0004.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3729-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ž. Gregáňová Author-Workplace-Name: Department of Biochemistry and Biotechnology, Slovak Agricultural University in Nitra, Nitra, Slovak Republic; 2Department of Applied Biochemistry and Molecular Biology, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: J. Kraic Author-Workplace-Name: Department of Biochemistry and Biotechnology, Slovak Agricultural University in Nitra, Nitra, Slovak Republic; 2Department of Applied Biochemistry and Molecular Biology, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: Z. Gálová Author-Workplace-Name: Department of Biochemistry and Biotechnology, Slovak Agricultural University in Nitra, Nitra, Slovak Republic; 2Department of Applied Biochemistry and Molecular Biology, Research Institute of Plant Production, Piešťany, Slovak Republic Title: Diagnostic of Wheat Leaf Rust Resistance Genes by DNA Markers and their Application in MAS - Short Communication Abstract: Leaf rust caused by Puccinia triticina belongs to the most important wheat diseases in Europe, including Slovakia. Winter wheat varieties registered in Slovakia have been tested with already developed STS (Lr9, Lr24), SCAR (Lr35) and SSR (Lr13) markers which are linked to the mentioned leaf rust resistance genes. Each of the four DNA markers linked to the individual resistance gene can be detected safely, easily and relatively fast by using the PCR reaction. This is very important for marker assisted selection (MAS) for the incorporation of the Lr9, Lr24 a Lr35 genes into chosen wheat genotypes with good bread-making quality or for the characterisation of wheat genetic resources (Lr13 gene). Keywords: Lr genes, PCR, wheat, Puccinia triticina, MAS Journal: Czech Journal of Genetics and Plant Breeding Pages: 127-129 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3730-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3730-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200304-0005.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3730-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Horáček Author-Workplace-Name: AGRITEC, Ltd., Šumperk, Czech Republic Author-Name: M. Ačanová Author-Workplace-Name: AGRITEC, Ltd., Šumperk, Czech Republic Title: Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication Abstract: In breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one dominant restorer gene allele (Rfrf or RfRf). To grow plants till the flowering stage takes much time. The method was therefore modified using isozyme markers of glucose-6-phosphate isomerase (PGI) to distinguish male sterile (MS) from male-fertile lines in early stages. Since the restorer gene is tightly linked to the markers and the PGI isozymes can be distinguished by electrophoresis, the markers can be used to identify MS rape plants in early stages. Also, homozygous and heterozygous fertility-restored plants can be separated this way. In our work we tried to optimise the distinction of pollen-fertile and pollen-sterile rape plants with PGI isozyme markers, using vertical polyacrylamide gel electrophoresis (native-PAGE). The method will be used for the breeding of rapeseed with the OGU-INRA system. Keywords: Brassica napus, winter rape, Ogura cytoplasmic male-sterility restorer gene, isozyme, PGI (glucose-6-phosphate isomerase) Journal: Czech Journal of Genetics and Plant Breeding Pages: 130-133 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3731-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3731-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200304-0006.txt Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:3731-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: K. Vaculová Title: Rex B. - Breeding for quantivative traits in plants - Book Review Journal: Czech Journal of Genetics and Plant Breeding Pages: 134-135 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/6103-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6103-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:6103-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 39 Journal: Czech Journal of Genetics and Plant Breeding Pages: X1 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/6104-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6104-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:39:y:2003:i:4:id:6104-CJGPB