Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of volume 40 Journal: Czech Journal of Genetics and Plant Breeding Pages: 91-101 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/6095-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6095-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:6095-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Gubiš Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: M. Hudcovicová Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: L. Klčová Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: V. Červená Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: K. Bojnanská Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: J. Kraic Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Title: Detection of Leaf Blotches - Causal Agents in Barley Leaves and Grains Abstract: The objective of this paper was to adapt PCR-based detection method for R. secalis and P. teres DNA isolated from pathogens and also from artificially infected juvenile leaves and seeds using pathogen-specific primers. It has been proven that primers specific to P. teres and R. secalis can reliably diagnose pathogen DNA as well as its presence in the mixture with barley DNA. Two primers set for detection of R. secalis were compared. The intensity of the corresponding DNA band after amplification with primer pair RS1-RS3 was higher than that amplified with RS8-RS9. The primer set RS1-RS3 was also used to detect R. secalis in barley seeds. DNA from infected seeds was isolated by two ways - according to the method of Dellaporta et al. (1983) or by the Adgen DNA Extraction System. The DNA extracted using the Adgen kit showed higher quality, however the amplification of the pathogen DNA was accomplished in both cases. Keywords: Pyrenophora teres, Rhynchosporium secalis, DNA, PCR, pathogen diagnostics Journal: Czech Journal of Genetics and Plant Breeding Pages: 111-117 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/3708-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3708-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200404-0002.txt Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3708-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: M. Žáková Author-Workplace-Name: Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: M. Benková Author-Workplace-Name: Research Institute of Plant Production, Piešťany, Slovak Republic Title: Genetic Diversity of Genetic Resources of Winter Barley Maintained in the Genebank in Slovakia Abstract: A set of 140 winter barley genetic resources of foreign and domestic origins was tested on experimental basis of RIPP in 1997-1999 to characterise the variability of the accessions based on agronomic data using multivariate methods. In the set tested, variability was studied of selected traits and characteristics such as: plant height (PH), weight of 1000 grains (W), grain number per a spike (SNG), grain uniformity - ratio of front seeds over 2.5 m sieve (GU), vegetation period - sowing/full maturity (VM) and seed yield (Y). Agronomic characters show great variability between cultivars. The study of matrix interrelationships between different variables showed that the yield is greatly correlated with traits: vegetation period - sowing/full maturity, grain uniformity and grain number per a spike. High positive correlation was obtained between the grain uniformity and the weight of 1000 grains. Negative correlation was found between the grain number per a spike and weight of 1000 grains in six-row barley. Correlations between agronomic traits differed between two- and six-row barley sets. The study revealed the existence of genetic differences among accessions as well as differences between two- and six-row winter barley and between the genotypes of domestic and foreign country origin, respectively. Results of this study provided information about diversity which should be of particular interest for the further collecting of genetic resources. Keywords: barley, Hordeum vulgare L., diversity, morphology, variation, agronomic characters, cluster analyses, principal component, cultivars Journal: Czech Journal of Genetics and Plant Breeding Pages: 118-126 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/3709-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3709-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200404-0003.txt Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3709-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Kučera Author-Workplace-Name: Department of Applied Genetics, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic, Author-Name: E. Schwarzbach Author-Workplace-Name: Department of Applied Genetics, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic, Author-Name: M. Klíma Author-Workplace-Name: Department of Applied Genetics, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic, Author-Name: M. Vyvadilová Author-Workplace-Name: Department of Applied Genetics, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic, Title: Agronomic Performance of Doubled Haploid Lines and Pedigree-Derived Lines of Winter Oilseed Rape Abstract: In four not overlapping sets of cultivars, including doubled haploid (DH) lines and pedigree derived (PD) lines of winter oilseed rape, both types of lines were compared in basic agronomic traits. The tested lines originated from breeding programmes of different breeders. Each set of cultivars was tested in a different year from 2001 to 2004 in multi-location trials with four replicates. There was no consistency in yield superiority of DH or PD lines and in all four sets the differences between the means of DH and PD groups were insignificant. On average, the differences between the groups of DH and PD lines were negligible in all evaluated agronomic traits. The statistical analyses of yield performance however revealed a very low predictive value of the multi-location trials. It can be concluded that with the given selection techniques and the actual system of cultivar trials nearly random samples of possible DH and PD lines were obtained in which no superiority of DH or PD lines in the evaluated agronomic traits could be detected. Keywords: winter oilseed rape, doubled haploid, seed yield, disease resistance Journal: Czech Journal of Genetics and Plant Breeding Pages: 127-133 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/3710-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3710-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200404-0004.txt Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3710-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: K. Chabane Author-Workplace-Name: J. C. K CHABANE and J VALKOUN Author-Name: J. Valkoun Author-Workplace-Name: J. C. K CHABANE and J VALKOUN Title: Characterisation of Genetic Diversity in ICARDA Core Collection of Cultivated Barley (Hordeum vulgare L.) Abstract: Out of the total accessions of cultivated barley, held at ICARDA, a subset core collection consisting of 153 accessions originating from different countries was established. Genetic diversity of the core collection was studied using AFLP markers. The accessions were grouped into different geographic sub-regions and the total genetic variation was estimated using Popgene software. Genetic distance matrix was computed and hierarchical unrooted tree was performed using Phylip software package. Our results demonstrate that the AFLP markers were highly informative and were useful in generating a meaningful classification of the cultivated barley that we determined as a subset of core collection. Keywords: barley, Hordeum vulgare L., genetic diversity, AFLP markers Journal: Czech Journal of Genetics and Plant Breeding Pages: 134-136 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/3711-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3711-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3711-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: K. Kosař Author-Workplace-Name: of the Czech-type Beer Author-Name: V. Psota Author-Workplace-Name: of the Czech-type Beer Author-Name: A. Mikyška Author-Workplace-Name: of the Czech-type Beer Title: Barley Varieties Suitable for Production of the Czech-type Beer Abstract: In the course of the 20th century, the production of beer was substantially changed. Economic pressures led to the concentration, modernisation and automation of the beer and malt production. These changes were reflected in a new view of malting barley varieties' quality. In two last decades, the varieties possessing a strong enzymatic activity, high extract contents and high final attenuation have been promoted. Due to historical reasons, the traditional production of pale lager has been preserved in the Czech brewing. For the production of the Czech-type beer, those varieties of malting spring barley are suitable that enable the production of beer with a higher level of residual extract, strong and full palate, excellent foaming power, and relatively lower alcohol content. Keywords: barley, malt, Czech-type beer Journal: Czech Journal of Genetics and Plant Breeding Pages: 137-139 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/3712-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3712-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200404-0006.txt Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3712-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: S.E. Germán Author-Workplace-Name: S E. GERMÁN Title: Breeding Malting Barley under Stress Conditions in South America Abstract: The annual average area sown with barley (Hordeum vulgare) in South America during 1999-2003 was 795 000 ha. In Argentina, Brazil, Chile and Uruguay, two-rowed spring cultivars are used mostly for malt production. Research has been developed in private malting companies and official institutions supported by the industry. In Argentina, tolerance to drought and heat stress during grain filling are important in drier areas. Yield and malt extract had been improved in cultivars released from 1940 to 1998. In Brazil, progress in grain yield, grain size, malting quality, early maturity, and resistance to net blotch, powdery mildew, and leaf rust has been achieved by EMBRAPA and malting companies. Higher tolerance to soil acidity and resistance to spot blotch are required. Since 1976, malting barley breeding in INIA-Chile has improved grain yield, grain size, beer production efficiency, and resistance to scald, net blotch, stripe rust, and leaf rust. Uruguay produces high quality malt exported mainly to Brazil. Malting companies have released locally bred and introduced cultivars since the early 1970's. Initiated in 1988, INIA-Uruguay breeding program has improved yield, malting quality, and lodging and disease resistance. Fusarium head blight is a new challenge for research in Brazil and Uruguay. Information regarding malting barley production, the most important stresses in different areas of production, and breeding progress under South American conditions is provided. Keywords: Hordeum vulgare L., plant breeding, stress conditions, Argentina, Brazil, Chile, Uruguay Journal: Czech Journal of Genetics and Plant Breeding Pages: 140-147 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/3713-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3713-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200404-0007.txt Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3713-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Špunar Title: 9th International Barley Genetics Symposium 20-26 June, 2004, Brno, Czech Republic Journal: Czech Journal of Genetics and Plant Breeding Pages: 148 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/6093-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6093-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:6093-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Hartmann Title: Ing. Josef Pešek, DrSc., seventieth birthday anniversary Journal: Czech Journal of Genetics and Plant Breeding Pages: 149 Volume: 40 Issue: 4 Year: 2004 DOI: 10.17221/6094-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6094-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:6094-CJGPB