Template-Type: ReDIF-Article 1.0 Author-Name: A E. Martínez Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Workplace-Name: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Author-Name: A Landau Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Workplace-Name: Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina Author-Name: P T. García Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Name: G Polenta Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Name: M C. Arias Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Name: R Murray Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Name: N Pensel Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Author-Name: A R. Prina Author-Workplace-Name: Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina Title: Two Mutants Affecting Adaptative Responses to Abiotic Stresses in Barley Seedlings Abstract: Two novel mutants which affect the adaptative responses of barley seedlings to different abiotic stresses are described. They allow us to explore some aspects of adaptative phenomena that are little known in higher plants. One of these mutants corresponds to a nuclear gene which under certain circumstances in the wild type barley induces additional ethylene production in the seedling roots. This mechanism seems to be involved in inducing a negative hydrotropic growth of the roots, a phenomenon that we interpret as a response avoiding waterlogging. The other mutant corresponds to a plastid encoded gene which is involved in photosystem I and II stability and, probably, indirectly affects the acclimation of the seedlings to higher temperatures, a fact which seems to occur through the control of unsaturation/saturation levels of the thylakoid membrane fatty acids. Keywords: abiotic stress, barley, root behaviour, temperature sensitivity Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-10 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/3675-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3675-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200501-0001.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:3675-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: L Bettaieb-Ben Kaab Author-Workplace-Name: Department of Biology, Faculty of Science of Tunis, Campus University, El Manar, Tunisia Author-Name: M El Felah Author-Workplace-Name: Department of Genetics, INRAT, Tunisia, Tunisia Author-Name: M Lauriere Author-Workplace-Name: Centre of Biotechnology Agro-Industry, INRA, Thiverval-Grignon, France$2 Title: Relationships Between Some Hordein Components and Quality Properties in Two Tunisian Barley Varieties as Influenced by Nitrogen Fertilisation Abstract: Two Tunisian barley varieties, Martin and Rihane, differing by their degrees of adaptation to the environmental conditions and grain yield potential, were grown under different levels of nitrogen (N) fertilisation. The effect of nitrogen supply on the hordein components (expressed in mg per albumen) was determined by Nu-PAGE electrophoresis system using the MOBS running buffer and densitometric analysis. Increasing nitrogen fertilisation increased the protein content by increasing the amount of C-hordeins. The sub-fraction BIII of high molecular weight B-hordeins appeared, in the present study, as the best criterion for distinguishing between the two barley varieties genetic adaptation, and its quantification could be recommended for the understanding of the quality properties of the barley response to different environmental conditions particularly nitrogen fertilisation which appears to be the main origin of differences in the protein aggregation mode of different B-hordein sulphur-rich sub-fractions. Keywords: barley, hordein components, Nu-PAGE, adaptation, N fertilisation Journal: Czech Journal of Genetics and Plant Breeding Pages: 11-16 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/3676-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3676-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200501-0002.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:3676-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: K Neplechová Author-Workplace-Name: Laboratory of Functional Genomics and Proteomics, Masaryk University in Brno, Brno, Czech Republic Author-Name: E Sýkorová Author-Workplace-Name: Laboratory of Functional Genomics and Proteomics, Masaryk University in Brno, Brno, Czech Republic Author-Workplace-Name: Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic Author-Name: M Novotná Author-Workplace-Name: Laboratory of Functional Genomics and Proteomics, Masaryk University in Brno, Brno, Czech Republic Author-Workplace-Name: Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic Author-Name: J Ptáček Author-Workplace-Name: Potato Research Institute, Havlíčkův Brod, Czech Republic Author-Name: J Fajkus Author-Workplace-Name: Laboratory of Functional Genomics and Proteomics, Masaryk University in Brno, Brno, Czech Republic Author-Workplace-Name: Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic Title: Structural Variability of Subtelomeres Applied for Genotyping of Selected Potato Varieties Abstract: Subtelomeric regions reveal a relatively high degree of polymorphism due to the increased frequency of recombination events in these chromosome loci. In a search for molecular markers applicable to genotyping of potato varieties, we focused on two possible sources of polymorphism occurring in this region: (i) arrangement of blocks of subtelomeric chromatin; (ii) structure of telomere-subtelomere boundary. Analysis of the internal arrangement of subtelomeric sequences showed several types of cultivar-specific spectra of PCR products arising from the variant orientation of sequence units of the ST3-subtelomeric sequence, or from different lengths of regions linking the individual sequence units. Further, the telomere-subtelomere boundary sequences were amplified using telomeric and ST3-specific primers and the obtained products were cloned. Sequence analysis of the clones resulted in characterisation of a novel telomere-associated sequence (FIN2). Primers derived from this sequence were then used alone or in combination with telomeric or ST3-specific primers to generate cultivar-specific spectra of PCR products. The described combinations of sequence-specific primers may be used for the fast, cheap and reproducible PCR-genotyping of selected potato varieties. Keywords: genotyping, potato, PCR, subtelomere, telomere-associated DNA sequences Journal: Czech Journal of Genetics and Plant Breeding Pages: 17-22 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/3677-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3677-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200501-0003.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:3677-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: M T Azhar Author-Workplace-Name: Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture, Faisalabad (Pakistan) Author-Name: A A Khan Author-Workplace-Name: Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture, Faisalabad (Pakistan) Author-Name: I A Khan Author-Workplace-Name: Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture, Faisalabad (Pakistan) Title: Combining Ability Analysis of Heat Tolerance in Gossypium hirsutum L. Abstract: In order to study the genetic control of heat tolerance, four varieties/lines of Gossypium hirsutum L., namely MNH-554, Cocker-304, Delcerro and Albacala (71) 1190, were crossed in all possible combinations. Heat tolerance of 12 hybrids and their parents was measured at reproductive phase using an electrolyte leakage technique. The genetic analysis of the data showed significant effects of specific combining ability and reciprocals, but the effect of general combining ability appeared to be non-significant. Comparison of the parents for heat tolerance revealed that Delcerro and MNH-554 were the best general combiners for the character. The crosses Cocker-304 × Delcerro and Albacala (71) 1190 × MNH-554 were determined as the best combinations for heat tolerance and they involved one good general combiner as a parent. Significant effects due to specific combining ability indicated the importance of non-additive gene effects controlling heat tolerance. This information suggests that development of heat tolerance in the present plant material may be difficult; therefore it is necessary to design a specific breeding programme for efficient transfer of the trait. Keywords: combining ability, genetic basis, Gossypium hirsutum, heat tolerance, membrane thermal stability Journal: Czech Journal of Genetics and Plant Breeding Pages: 23-28 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/3678-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3678-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200501-0004.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:3678-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Ing. Ivo Bareš, DrSc., is eighty Journal: Czech Journal of Genetics and Plant Breeding Pages: 29-30 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/6070-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6070-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:6070-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Z. Ohnoutka Title: Winter Wheat Evelina Journal: Czech Journal of Genetics and Plant Breeding Pages: 31-32 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/6071-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6071-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:6071-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Z. Ohnoutka Title: Winter Wheat Ines Journal: Czech Journal of Genetics and Plant Breeding Pages: 33-34 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/6072-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6072-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:6072-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Houdek Title: × Festulolium Perseus Journal: Czech Journal of Genetics and Plant Breeding Pages: 35-36 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/6073-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6073-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:6073-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Houdek Title: Red Fescue Fantasie Journal: Czech Journal of Genetics and Plant Breeding Pages: 37 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/6074-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6074-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:6074-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Houdek Title: Red Fescue Fidelio Journal: Czech Journal of Genetics and Plant Breeding Pages: 38 Volume: 41 Issue: 1 Year: 2005 DOI: 10.17221/6075-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6075-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:1:id:6075-CJGPB