Template-Type: ReDIF-Article 1.0 Author-Name: A Graner Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: T Thiel Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: H Zhang Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: E Potokina Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: M Prasad Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: D Perovic Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: R Kota Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: R K Varshney Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: U Scholz Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: I Grosse Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: N Stein Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Title: Molecular Mapping in Barley: Shifting from the Structural to the Functional Level Abstract: As a resource for structural and functional barley genome analysis, more than 140 000 ESTs (expressed sequence tags) were generated from 22 cDNA libraries that yielded 25 224 tentative unigenes. About 50% of them belong to gene families. The size of the complete transcriptome is estimated to comprise between 35 000 and 75 000 genes. The barley EST collection is a rich source for the development of novel markers including SSRs (simple sequence repeats) and SNPs (single nucleotide polymorphisms). Several bioinformatic tools have been developed facilitating the computer-assisted analysis of EST databases for the presence of either SNPs or SSRs and the development of SNP-derived CAPS (cleaved amplified polymorphic sequences) markers. In an attempt to systematically map barley genes a high-density transcript map is under construction and presently comprises more than 1000 markers. This map is a gateway to comparative genomics with particular emphasis on the rice genome. 65% of the mapped ESTs showing a significant homology to rice ESTs were found to display a syntenic relationship between barley and rice. Thus, the barley EST resource facilitates the rapid and systematic transfer of genetic information from rice to barley and other Triticeae, which can readily be exploited for marker saturation of defined chromosome regions and their detailed comparison to rice. In the context of a functional genomics study, the complex trait "malting quality" is investigated using a barley cDNA array. By correlating the phenotypic malting trait data of selected barley lines with the corresponding expression profiles, a set of candidate genes was identified and further verified by genetic analysis. Keywords: ESTs, functional genomics, malting quality, DNA-marker, rice, synteny Journal: Czech Journal of Genetics and Plant Breeding Pages: 81-88 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/3665-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3665-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200503-0001.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:3665-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lars MUNCK Author-Workplace-Name: Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark Author-Name: Birthe MØLLER Author-Workplace-Name: Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark Title: Principal Component Analysis of Near Infrared Spectra as a Tool of Endosperm Mutant Characterisation and in Barley Breeding for Quality Abstract: Near infrared technology, now widespread in quality control, makes it possible to obtain a total multivariate physical chemical fingerprint of the barley endosperm with high precision. Whole spectroscopic fingerprints of the physics and chemistry of barley seeds can be interpreted by multivariate analysis (chemometrics), by Principal Component Analysis (PCA) for classification and Partial Least Squares Regression (PLSR) for correlation. PCA classification of Near Infrared Reflectance (NIR) spectra can differentiate between mutants and alleles in the lys3 and lys5 loci. PCA on NIR can also be used as a routine in barley breeding to select for a multi-gene quality complex in barley as a whole e.g. increasing starch and reducing fibre content. This is done directly from the PCA classification plot by "data breeding" selecting the recombinants which are approaching the position of the normal high starch controls on the plot. Based on classification of NIR spectra, two alleles in the lys5 locus were characterised as a new class of (1→3,1→4)-β-glucan compensating starch mutants indicating a metabolicconnection between starch and β-glucan. compensating starch mutants indicating a metabolic connection between starch and β-glucan. Keywords: multivariate data analysis, NIR spectroscopy, barley mutants, physical-chemical fingerprint Journal: Czech Journal of Genetics and Plant Breeding Pages: 89-95 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/3666-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3666-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200503-0002.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:3666-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: D Mikulíková Author-Workplace-Name: Institute of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: I Čičová Author-Workplace-Name: Institute of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: G Antalíková Author-Workplace-Name: Institute of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Author-Name: J Kraic Author-Workplace-Name: Institute of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic Title: Grains of Nontraditional Crops as Sources of Retrograded Resistant Starch Abstract: The content of retrograded resistant starch of the RS3 type was determined in six plant species and their accessions. Besides sorghum and foxtail millet, plant genotypes were cultivated in Piešťany locality in the year 2003. There were significant differences in RS3 and total starch content in analysed crops and their accessions. Chickpea and buckwheat had the highest average content of RS3, 5.2% and 3.8%, respectively. Millet and foxtail millet contained less RS3, 2.9% and 2.8%, respectively. Millet was rich in total starch. The lowest amounts of RS3 were detected in sorghum (1.6%) and amaranth (1.2%). The lowest variation in the RS3 content was detected within the set of millet accessions and the highest within amaranths. Keywords: resistant starch, retrograded starch, amaranth, buckwheat, chickpea, foxtail millet, millet, sorghum Journal: Czech Journal of Genetics and Plant Breeding Pages: 96-104 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/3667-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3667-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200503-0003.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:3667-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: R Koprna Author-Workplace-Name: OSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech Republic Author-Name: V Kučera Author-Workplace-Name: Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: O Kolovrat Author-Workplace-Name: OSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech Republic Author-Name: M Vyvadilová Author-Workplace-Name: Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: M Klíma Author-Workplace-Name: Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding Abstract: Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified. Keywords: oilseed rape (Brassica napus L. var. napus), hybrid breeding, self-incompatibility, seed quality, glucosinolates, erucic acid Journal: Czech Journal of Genetics and Plant Breeding Pages: 105-111 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/3668-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3668-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200503-0004.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:3668-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: L. Věchet Author-Name: M. Vojáčková Title: Use of Detached Seedling Leaf Test to Evaluate Wheat Resistance to Septoria Tritici Blotch Journal: Czech Journal of Genetics and Plant Breeding Pages: 112-116 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/3669-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3669-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:3669-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Horáčková Author-Name: J. Domkářová Title: The Czech Bank Of Potato Genetic Resources Journal: Czech Journal of Genetics and Plant Breeding Pages: 117-119 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/6078-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6078-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:6078-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Memorandum Of The Participants Of The Seminar "New Knowledge In Plant Biology And Its Use In Solving The Needs Of Plant Breeding", Organised In Response To The European Biotechnology Platform "Plants For The Future" Journal: Czech Journal of Genetics and Plant Breeding Pages: 120-121 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/6079-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6079-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:6079-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Svačina Title: Spring Barley Bojos Journal: Czech Journal of Genetics and Plant Breeding Pages: 122-123 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/6080-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6080-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:6080-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Svačina Title: Spring Barley Radegast Journal: Czech Journal of Genetics and Plant Breeding Pages: 124-125 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/6081-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6081-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:6081-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Houdek Title: Common Bentgrass Polana Journal: Czech Journal of Genetics and Plant Breeding Pages: 126 Volume: 41 Issue: 3 Year: 2005 DOI: 10.17221/6082-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6082-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:6082-CJGPB