Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 41 Journal: Czech Journal of Genetics and Plant Breeding Pages: 96-104 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/6087-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6087-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:6087-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V Šíp Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: P Bartoš Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: J Chrpová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: A Hanzalová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: L Širlová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: J Šárová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: V Dumalasová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: L Čejka Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: A Hanišová Author-Workplace-Name: SELGEN, a. s., Breeding Station Stupice, Czech Republic Author-Name: L Bobková Author-Workplace-Name: SELGEN, a. s., Breeding Station Úhřetice, Czech Republic Author-Name: I Bížová Author-Workplace-Name: SELGEN, a. s., Breeding Station Úhřetice, Czech Republic Author-Name: P Horčička Author-Workplace-Name: SELGEN, a. s., Breeding Station Stupice, Czech Republic Title: Theoretical Bases and Sources for Breeding Wheat for Combined Disease Resistance Abstract: Achievements and prospects of wheat breeding for disease resistance in the world and in theCzech Republic are discussed. Attention was paid to possibilities of increasing resistance to rusts, powdery mildew, Fusarium head blight, leaf blotch, glume blotch, tan spot, common bunt and barley yellow dwarf virus on wheat. Methodical approaches adopted in national ring infection tests were outlined. New sources of resistance to the above-mentioned diseases were detected and described on the basis of three-year results of field infection tests. Keywords: wheat, Triticum aestivum L., sources of resistance, resistance genes, fungal diseases, virus diseases, Czech Republic Journal: Czech Journal of Genetics and Plant Breeding Pages: 127-143 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/3659-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3659-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200504-0002.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:3659-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: T J. Close Author-Workplace-Name: Department of Botany & Plant Sciences, University of California, Riverside, CA, USA Title: The Barley Microarray. A Community Vision and Application to Abiotic Stress Abstract: A microarray chip representing approximately 20 000 barley unigenes was produced as part of a USA project entitled "An integrated physical and expression map of barley for Triticeae improvement". The content of the chip was derived from more than 400 000 barley "Expressed Sequence Tag" (EST) sequences received from cooperators inUSA,Germany,Australia,Japan,Scotland, andFinland, plus about 1000 sequences retrieved from the GenBank nr database or GrainGenes. All EST sequences were trimmed to high quality regions, contaminants were identified and removed, and the remaining information was compiled using the CAP3 assembly program. A "stringent" assembly (paralogs separated) contained about 53 000 "unigenes" (the sum of contigs plus singletons), among which about 50% had reliable 3' ends and were therefore suitable for chip content. From these, probe sets were designed and the "Barley1" chip fabricated by Affymetrix. Complete details on the content of the barley microarray, and enhanced probe-set annotations, can be obtained using the software HarvEST:Barley, available from http://harvest.ucr.edu. The availability of an Affymetrix barley microarray has facilitated the study of gene expression on a large scale. Replicated experiments have revealed commonalities and differences between responses to abiotic stresses, and inherent differences between barley genotypes. The design and a brief summary of the results of drought stress experiments are stated. Keywords: genomics, ESTs, microarray, abiotic stress Journal: Czech Journal of Genetics and Plant Breeding Pages: 144-152 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/3660-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3660-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200504-0003.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:3660-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: R K Varshney Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: U Hähnel Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: T Thiel Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: N Stein Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: L Altschmied Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: P Langridge Author-Workplace-Name: Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, PMB1 Glen Osmond, Australia Author-Name: A Graner Author-Workplace-Name: Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Title: Genetic and Physical Mapping of Genic Microsatellites in Barley (Hordeum vulgare L.) Abstract: Due to the availability of sequence data from large-scale EST (expressed sequence tag) projects, it has become feasible to develop microsatellite or simple sequence repeat (SSR) markers from genes. A set of 111 090 barley ESTs (corresponding to 55.9 Mb of sequence) was employed for the identification of microsatellites with the help of a PERL5 script called MISA. As a result, a total of 9 564 microsatellites were identified in 8 766 ESTs (SSR-ESTs). Cluster analysis revealed the presence of 2 823 non-redundant SSR-ESTs in this set. From these 754 primer pairs were designed and analysed in a set of seven genotypes including the parents of three mapping populations. Finally, 185 microsatellite (EST-SSRs) loci were placed onto the barley genetic map. These markers show a uniform distribution on all the linkage groups ranging from 21 markers (on 7H) to 35 markers (3H). The polymorphism information content (PIC) for the developed markers ranged from 0.24 to 0.78 with an average of 0.48. For the assignment of these markers to BAC clones, a PCR-based strategy was established to screen the "Morex"-BAC library. By using this strategy BAC addresses were obtained for a total of 127 mapped EST-SSRs, which may provide at least two markers located on a single BAC. This observation is indicative of an uneven distribution of genes and may lead to the identification of gene-rich regions in the barley genome. Keywords: EST-SSRs, genic microsatellites, genetic mapping, physical mapping Journal: Czech Journal of Genetics and Plant Breeding Pages: 153-159 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/3661-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3661-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200504-0004.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:3661-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: A Dreiseitl Author-Workplace-Name: Agrotest, Agricultural Testing, Advisory Services and Research, Ltd., Kroměříž, Czech Republic Title: Powdery Mildew Resistance of Czech and Slovak Spring Barley Breeding Lines in Variety Trials Abstract: In 2001-2005, resistance to powdery mildew was studied in 227 Czech and Slovak breeding lines of spring barley included in the breeding station trials or official trials. Seventeen known resistances were identified (Al, Ar, At, HH, Kr, La, Ly, Mlo, N81, Ri, Ru, Sp, St, Tu, We, Mla21, and Mlp1). Unknown resistances were found in 11 breeding lines, in five of which resistance was effective against all used pathotypes of the pathogen. Besides the identified resistances, unknown resistances were detected in another three breeding lines. Sixty-five breeding lines (= 29%) exhibited heterogeneity in the examined trait, i.e. they are composed of components with different resistances to powdery mildew. Comparison of current results with the previous ones shows a considerable increase in the proportion of breeding lines carrying the resistance Mlo (72%), on the account of the resistances located at the Mla locus, particularly Ru. The examined set is characterised by a high proportion of breeding lines resistant to all used Czech pathotypes of the given pathogen (78%), however of low diversity in the resistance. Keywords: Blumeria graminis f.sp. hordei, Erysiphe graminis f.sp. hordei, powdery mildew, Hordeum vulgare L., spring barley, breeding lines, resistance Journal: Czech Journal of Genetics and Plant Breeding Pages: 160-166 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/3662-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3662-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200504-0005.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:3662-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J Salava Author-Workplace-Name: Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: J Polák Author-Workplace-Name: Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: B Krška Author-Workplace-Name: Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice, Czech Republic Title: Oligogenic Inheritance of Resistance to Plum Pox Virus in Apricots Abstract: In order to determine the inheritance of resistance to PPV in apricot three crosses between resistant and susceptible cultivars and selections were performed. The B1 seedlings were inoculated with the PPV-M strain by an infected bud. PPV infection was evaluated over 5 consecutive growth periods through visual symptoms, ELISA and in some cases reverse transcriptase PCR assays. Chi-square analysis of each B1 progeny was performed to determine if the segregation ratio differed from the expected ratio. PPV resistance segregated in three apricot B1progenies in a 1:7 (resistant:susceptible) ratio, indicating that resistance was controlled by three independent dominant complementary genes. All three dominant genes are needed for the resistance to be expressed, and the lack of any one of the dominant alleles will result in susceptibility. This knowledge will help us in effective planning of apricot breeding programs with this subjective. Keywords: Prunus armeniaca L., heritability, sharka disease Journal: Czech Journal of Genetics and Plant Breeding Pages: 167-170 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/3663-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3663-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200504-0006.txt Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:3663-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Voral Title: Potato Marcela Journal: Czech Journal of Genetics and Plant Breeding Pages: 171-172 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/6083-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6083-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:6083-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Voral Title: Potato Nikoleta Journal: Czech Journal of Genetics and Plant Breeding Pages: 173-174 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/6084-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6084-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:6084-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Voral Title: Potato Sázava Journal: Czech Journal of Genetics and Plant Breeding Pages: 175-176 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/6085-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6085-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:6085-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Černoch Title: Common Bentgrass Kuzma Journal: Czech Journal of Genetics and Plant Breeding Pages: 177 Volume: 41 Issue: 4 Year: 2005 DOI: 10.17221/6086-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6086-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:4:id:6086-CJGPB