Template-Type: ReDIF-Article 1.0 Author-Name: Jana Palicová-Šárová Author-Workplace-Name: Division of Plant Medicine and Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Alena Hanzalová Author-Workplace-Name: Division of Plant Medicine and Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Reaction of 50 Winter Wheat Cultivars Grown in the Czech Republic to Pyrenophora tritici-repentis Races 1, 3, and 6 Abstract: The reaction of 50 winter wheat cultivars/lines to artificial infection with Pyrenophora tritici-repentis (PTR) races 1, 3, and 6 was studied under greenhouse conditions. The set of tested cultivars/lines included predominantly cultivars registered in the Czech Republic and some new breeding lines. A high level of resistance to P. tritici-repentis was detected in the cultivars Clarus, Rheia, Cubus, SHMK WW 14-92, Šárka, Vlasta and Dromos (SWS 799.14953), susceptible reactions were observed in the cultivars Caphorn, Corsaire, Karolinum, Heroldo (PBIS 00/91), Hedvika, Biscay, Svitava, Barroko (PBIS 00/140) to all three races tested. The majority of the tested cultivars possess a moderate level of resistance to PTRraces 1, 3, and 6. Significant differences were proved not only in the reaction of the tested cultivars but also in the aggressiveness of the three used isolates. Keywords: Pyrenophora tritici-repentis, tan spot, winter wheat, resistance Journal: Czech Journal of Genetics and Plant Breeding Pages: 31-37 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/3651-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3651-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200602-0001.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:3651-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Antonín Dreiseitl Author-Workplace-Name: Agrotest fyto, Ltd., Kroměříž, Czech Republic Title: Powdery Mildew Resistance of Winter Barley Varieties in Czech Official Trials Abstract: In 1996-2005, resistance to powdery mildew was studied in 167 winter barley varieties (of them 83 two-rowed and 84 six-rowed, 45 Czech and 122 foreign ones) included in the Czech Official Trials in that period. Seventeen known resistances to powdery mildew were identified (Ar, Bw, Di, Dr, Dt, Ha, HH, Ch, IM9, La, Lu, Ly, Ra, Ru, Sp, St and We). Unknown resistances were found in 25 varieties, in six of which (= 3.6% of all the examined set) they were effective to all used pathotypes of the pathogen. Six varieties exhibited heterogeneity in the examined trait, i.e. they are composed of lines with different resistances to powdery mildew. The most frequent resistances in the set were Ra, which was detected in 89 varieties (= 53%), and Bw was found in 30 varieties (18%). The resistances Sp, Ly, Ha, HH and Ch were also frequent. However, the frequency of the last three resistances cannot be quantified since we were not able to identify these resistances during the whole examined period. Keywords: Blumeria graminis f.sp. hordei, Erysiphe graminis f.sp. hordei, Hordeum vulgare L Journal: Czech Journal of Genetics and Plant Breeding Pages: 38-43 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/3652-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3652-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200602-0002.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:3652-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Pavlína Hrstková Author-Workplace-Name: Department of Crop Science and Plant Breeding, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: Oldřich Chloupek Author-Workplace-Name: Department of Crop Science and Plant Breeding, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: Jitka Bébarová Author-Workplace-Name: Department of Crop Science and Plant Breeding, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Title: Estimation of Barley Seed Vigour with Respect to Variety and Provenance Effects Abstract: The study was aimed at determining potential differences in seed quality of spring and winter barley in relation to varieties (four winter and two spring barley varieties) and growing conditions (six locations, two years). Three tests were performed on each seed sample, i.e. (1) the standard germination test; (2) the low temperature and drought stress germination test and (3) the germination test in a salt solution. The effects of the factors of variety, provenance and year on seed vigour were estimated by the analysis of variance and Tukey's post hoc test. The germination of winter varieties was influenced by provenance, both measures of vigour (2 and 3) by year, and significant differences between varieties were found in all three traits. Spring varieties differed only in germination and vigour (3) in one year when the influence of provenance prevailed. The results show different effects of variety and provenance on seed vigour. This could be promising for the breeding of high vigour barley varieties. Some preliminary results were obtained by using controlled deterioration (CD) as a vigour testing method for barley. The suitable conditions seem to be the combination of 45°C and 24% moisture content for the period of 24 hours. Keywords: barley, germination, seed vigour, variety, provenance, controlled deterioration Journal: Czech Journal of Genetics and Plant Breeding Pages: 44-49 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/3653-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3653-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200602-0003.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:3653-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Václav Dvořáček Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jana Bradová Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Zdeněk Stehno Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Effect of 1B/1R Translocation on Selected Grain Quality Parameters in a Set of Doubled Haploid Wheat Lines Abstract: A set of quality parameters (crude protein content, Zeleny sedimentation test, wet gluten content, gluten index, albumins + globulins content, gliadin content, sum of glutenins, proportion of albumins + globulins in crude protein and relative viscosity) was tested in 17 doubled haploid (DH) wheat lines differing in the presence or absence of 1B/1R translocation. The presence of 1B/1R translocation (allele Gli 1B3) affected the significantly higher content of albumins and globulins and significantly lower value of gluten index. Nevertheless, a certain translocation influence on the other parameters (Zeleny sedimentation test; relative viscosity; proportion of albumins + globulins in crude protein) was also registered. The deteriorative effect of the 1B/1R translocation on indirect technological grain parameters was confirmed more markedly in gluten index than in Zeleny sedimentation test. A high number of significant differences between lines was found in Zeleny sedimentation test, content of glutenin, content of albumins + globulins and their proportion in crude protein. In spite of lower variability between lines relative viscosity showed a high dependence on genotype and was indifferent to the other quality parameters. Keywords: wheat, grain quality, Gli-/Glu-alleles, 1B/1R translocation Journal: Czech Journal of Genetics and Plant Breeding Pages: 50-57 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/3654-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3654-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200602-0004.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:3654-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Iva Smýkalová Author-Workplace-Name: Plant Biotechnology Department, AGRITEC Ltd., Šumperk, Czech Republic Author-Name: Martina Větrovcová Author-Workplace-Name: Plant Biotechnology Department, AGRITEC Ltd., Šumperk, Czech Republic Author-Name: Miroslav Klíma Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Ivana Macháčková Author-Workplace-Name: Plant Breeding Station Chlumec nad Cidlinou, SELGEN a. s., Chlumec nad Cidlinou, Czech Republic$2 Author-Name: Miroslav Griga Author-Workplace-Name: Plant Biotechnology Department, AGRITEC Ltd., Šumperk, Czech Republic Title: Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project "Czech Winter Rape" Abstract: A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project "Czech Winter Rape". All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled haploid), polyploid (triploid, tetraploid) and mixoploid (containing both euploid and aneuploid nuclei) plants. The fully aneuploid plants were recorded only sporadically. Some modifications of culture protocol (removal of embryo cotyledons before transfer to a germination medium; production of more plantlets from a single embryo by rooting multiple shoots; repeated colchicine treatment of unsuccessfully doubled plants in a later phase of growth) could enhance the final yield of DH fertile regenerants in our experiments. In total, 590 microspore-derived regenerants were produced in a series of experiments, 236 (40%) of them represented fully fertile DH plants. The R2 seeds (progeny of DH R1-generation) of the most promising hybrid-lines were included in the tests of agronomic performance in field conditions. Keywords: microspore culture, doubled haploids, winter rape, Brassica napus L., hybrid breeding Journal: Czech Journal of Genetics and Plant Breeding Pages: 58-71 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/3655-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3655-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200602-0005.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:3655-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Hartmann Title: RNDr. Erik Schwarzbach, Dr. agr. habil., Seventieth Birthday Anniversary Journal: Czech Journal of Genetics and Plant Breeding Pages: 72 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/6057-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6057-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:6057-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Horčička Author-Name: A. Hanišová Title: Winter Wheat Simila Journal: Czech Journal of Genetics and Plant Breeding Pages: 73-74 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/6058-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6058-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:6058-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Kreuzman Author-Name: M. Liška Title: Pea Variety Terno Journal: Czech Journal of Genetics and Plant Breeding Pages: 75-76 Volume: 42 Issue: 2 Year: 2006 DOI: 10.17221/6059-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6059-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:2:id:6059-CJGPB