Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 42 Journal: Czech Journal of Genetics and Plant Breeding Pages: 95-102 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/6063-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6063-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:6063-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zdeněk Stehno Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Iva Faberová Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Ladislav Dotlačil Author-Workplace-Name: Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Sergey Martynov Author-Workplace-Name: N.I. Vavilov Research Institute of Plant Industry, Sankt-Petersburg, Russia Author-Name: Tatjana Dobrotvorskaya Author-Workplace-Name: N.I. Vavilov Research Institute of Plant Industry, Sankt-Petersburg, Russia Title: Genealogical Analysis in the Czech Spring Wheat Collection and its Use for the Creation of Core Collection Abstract: A spring wheat collection of the Czech gene bank included altogether 3270 original accessions in 2005; among them 2123 accessions with pedigree were analyzed using the construction of genetic profiles and subsequent calculation of Renkonen similarity indexes. Genetic diversity was estimated by Shannon diversity index. Subsequently we calculated cluster analysis using coefficients of parentage and in addition, we selected representatives of particular clusters for a core collection. Accessions of the whole analysed set of spring wheat (2123) were progenies of 929 original ancestors and diversity index within the set was H = 4.17. In the selected core set we can trace 718 ancestors and the diversity index is H' = 4.82. The selected core set is composed of 645 accessions (i.e. 30.4% of the whole set) and it represents 77.3% of genetic diversity in the whole set. The diversity between clusters did not decrease in the core set, which shows the representativeness of selected core accessions. Genealogical analysis provided useful information for the choice of cultivars to a core collection. However, pedigree analysis has to be complemented by other generally applicable approaches such as the use of molecular markers, morphological and agronomical characters. The analysis can also provide valuable information for breeders and researchers. Keywords: Triticum aestivum L., pedigree analysis, genetic diversity, core collection Journal: Czech Journal of Genetics and Plant Breeding Pages: 117-125 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/3641-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3641-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200604-0002.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:3641-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Alena Hanzalová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Pavel Bartoš Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Physiologic Specialization of Wheat Leaf Rust (Puccinia triticina Eriks.) in theCzech Republic in 2001-2004 Abstract: In 2001-2004 virulence of the wheat leaf rust population in the Czech Republic was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust (180 in total) were obtained from different parts of the Czech Republic. Resistance genes Lr9 and Lr19 were effective to all tested isolates like in the previous years. Unlike the previous years virulence on Lr24 and Lr28 was observed. Relatively effective were also Lr1, Lr2a, Lr24, Lr28 and Lr2b. Other Lr genes were defeated by the majority of the tested samples. Our results transformed to the numbers of physiologic races indicate that race 61SaBa prevailed like in the previous years, followed by races 61, 2, 12SaBa, 2SaBa, 14, 77SaBa, 12, 57, 6, 53, 53SaBa, 77, and 14SaBa. Twenty-one winter wheat cultivars registered in 2001-2004 were tested with 8 leaf rust isolates. Out of them 15 showed resistance at least to one rust isolate. High resistance recorded in field trials for cvs Batis and Ilias, which were susceptible to all tested rust isolates at the seedling stage, demonstrates the importance of field (partial) resistance. Keywords: Lr genes, physiologic races, resistance, winter wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 126-131 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/3642-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3642-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200604-0003.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:3642-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Radoslav Koprna Author-Workplace-Name: OSEVA PRO Ltd., dep. Research Institute of Oilseed Crops at Opava, Opava, Czech Republic Author-Name: Pavel Nerušil Author-Workplace-Name: Research Station of Grass Ecosystems at Jevíčko, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Oldřich Kolovrat Author-Workplace-Name: OSEVA PRO Ltd., dep. Research Institute of Oilseed Crops at Opava, Opava, Czech Republic Author-Name: Vratislav Kučera Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Alois Kohoutek Author-Workplace-Name: Research Station of Grass Ecosystems at Jevíčko, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Estimation of Fatty Acid Content in Intact Seeds of Oilseed Rape (Brassica napus L.) Lines Using Near-Infrared Spectroscopy Abstract: Based on NIRS (near infrared reflectance spectroscopy) measurements carried out in a collection of 262 samples of winter oilseed rape with a different content of fatty acids (FA) in oil, calibration equations for the laboratory instrument Foss-NIRSystem 6500 were developed. Calibration was focused on the possibility of screening seed samples of different composition of oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3) using NIRS analysis. The reference method was gas chromatography (GC). The content of FA in segregating F2 generations after crossing between lines with different contents of FA and lines with standard content of FA in oil ranged from 32.3 to 82.0% for C18:1, 10.2-26.8% for C18:2 and 3.3-11.8% for C18:3. The verification of a validation equation in 50 randomly selected samples of F2 generation in the year 2006 proved high correlation coefficients (r) between NIRS analysis and GC values, r = 0.86 for C18:1, r = 0.82 for C18:2 and r = 0.85 for C18:3. Non-destructive NIRS analysis enables rapid and reliable selection of materials with different composition of FA in the seed of oilseed rape (lines with desirable high content of C18:1 and low content of C18:3). Keywords: winter oilseed rape, fatty acids, oleic acid, linoleic acid, linolenic acid, NIRS calibration Journal: Czech Journal of Genetics and Plant Breeding Pages: 132-136 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/3643-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3643-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200604-0004.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:3643-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jana Chrpová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Václav Šíp Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Eva Matějová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Světlana Sýkorová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Progression of Deoxynivalenol Concentrations in Spikes and Kernels of Winter Wheat Cultivars after Inoculation with Fusarium culmorum Abstract: Progression of deoxynivalenol (DON) concentrations in spikes and kernels was studied in relation to Fusarium head blight (FHB) symptoms in five winter wheat cultivars, differing in resistance to FHB, after single floret inoculation with an aggressive isolate of Fusarium culmorum. After inoculation in field conditions the spikes were detached from the plant and kept in the greenhouse under controlled conditions. High concentrations of DON were detected in susceptible cultivars at an early stage of pathogenesis (7 days after inoculation). Over the whole examined 21-day period and also at maturity spikes contained more DON than kernels. While differences between cultivars in the accumulation of DON were highly expressed already 7 days after inoculation, differences in symptomatic reactions were not clear until day 21. Owing to the reported crucial role of DON at early stages of pathogenesis, the importance of appropriate timing of fungicide application is highly stressed. Keywords: Fusarium head blight, symptoms, accumulation of deoxynivalenol, resistance, winter wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 137-141 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/3644-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3644-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200604-0005.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:3644-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Světlana Sýkorová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Eva Matějová Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Characterisation of Potato (Solanum tuberosum L.) Varieties by Electrophoresis of Tuber Proteins Abstract: The modified PAGE method (TRIS - Glycine buffer pH 8.9) was used for the characterisation of selected 25 registered potato varieties. This method enabled to identify each variety from the examined variety set. The calculation of identity indexes (proportion of common bands) helped to evaluate the similarity of varieties from these aspects. The examination of electrophoretic profiles of soluble tuber proteins, which are highly polymorphic and stable, can be considered as valuable for variety characterization and identification. Keywords: potato, variety, characterisation, tuber proteins, electrophoresis Journal: Czech Journal of Genetics and Plant Breeding Pages: 142-146 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/3645-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3645-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200604-0006.txt Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:3645-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Šíp Author-Name: J. Chrpová Author-Name: L. Bobková Title: Winter Wheat Raduza Journal: Czech Journal of Genetics and Plant Breeding Pages: 147-148 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/6061-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6061-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:6061-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Čapek Title: White Mustard Polarka Journal: Czech Journal of Genetics and Plant Breeding Pages: 149-150 Volume: 42 Issue: 4 Year: 2006 DOI: 10.17221/6062-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6062-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:6062-CJGPB