Template-Type: ReDIF-Article 1.0 Author-Name: Pavel Vítámvás Author-Workplace-Name: Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Klára Kosová Author-Workplace-Name: Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Ilja T. Prášil Author-Workplace-Name: Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Proteome analysis in plant stress research: a review Abstract: Proteomic techniques that allow the identification and quantification of stress-related proteins, mapping of dynamics of their expression and posttranslational modifications represent an important approach in the research of plant stresses. In this review, we show an outline of proteomics methods and their applications in the research of plant resistance to various types of stresses. Keywords: plant proteomics, mass spectrometry, two-dimensional gel electrophoresis Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-6 Volume: 43 Issue: 1 Year: 2007 DOI: 10.17221/1903-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/1903-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200701-0001.txt Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:1:id:1903-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Pavol Múdry Author-Workplace-Name: Trnava University, Trnava, Slovak Republic Author-Name: Ján Kraic Author-Workplace-Name: SCPV-Research Institute of Plant Production, Piešťany, Slovak Republic Author-Workplace-Name: University of SS. Cyril and Methodius, Trnava, Slovak Republic Title: Inter- and intra-population variation of local maize (Zea mays L.) populations from Slovakia and Czech Republic Abstract: Evaluation of genetic variation was performed within 62 local maize populations originating from Slovakia and Czech Republic. In total 48 alleles at 22 analyzed isoenzyme loci with an average of 2.2 alleles per locus were revealed. The percentage of polymorphic loci ranged from 14% to 59% and the frequencies of detected alleles varied from null to four per locus. No polymorphism was detected at the loci Dia2, Got3, Mdh4, Mmm, and Pgm1. The highest number of alleles (four) was detected at loci Acp1, Cat3, Pgm2. No new alleles were identified, nevertheless the frequency of seven alleles was only about 1%. The expected heterozygosity ranged from null to 0.492 with an average of 0.197. The revealed isoenzyme polymorphism confirmed that all analyzed populations were heterogeneous and as many as 17 of them were completely heterogeneous. None of the analyzed populations was identical in the frequency of alleles at all 22 analyzed loci. Keywords: maize, local populations, isoenzyme, genetic diversity Journal: Czech Journal of Genetics and Plant Breeding Pages: 7-15 Volume: 43 Issue: 1 Year: 2007 DOI: 10.17221/1904-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/1904-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200701-0002.txt Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:1:id:1904-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Václav Šíp Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jana Chrpová Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Leona Leišová Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Světlana Sýkorová Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Ladislav Kučera Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jaroslava Ovesná Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Effects of genotype, environment and fungicide treatment on development of Fusarium head blight and accumulation of DON in winter wheat grain Abstract: Reactions to artificial infection with Fusarium culmorum and (metconazole- or tebuconazole-based) fungicides were studied in nine winter wheat cultivars that were evaluated in field experiments at the location Prague-Ruzyne for four years (2001-2004) for deoxynivalenol (DON) content in grain, pathogen DNA content (Ct) by real-time quantitative PCR, percentage of Fusarium damaged grains (FDG), symptom scores and reductions in grain yield components. All examined traits were highly affected by conditions of experimental years and interactions with cultivars and treatments. Moderately resistant cultivars Arina and Petrus were included in the first homogeneous group in all traits, including the pathogen DNA content. To predict cultivar resistance to Fusarium head blight and accumulation of DON, the examination of the percentage of FDG in different environments appeared to be useful from practical aspects. The pathogen DNA content was significantly related to the content of DON under different conditions, however, the correlation coefficients ranged between 0.42 and 0.92. Different levels of DON could be detected at similar pathogen contents. The higher colonization of grain by the fungus was mostly connected with a strongly reduced amount of DON per pathogen unit (DON/Ct ratio). The fungicide treatment had a significant effect on a reduction in all traits except DON/Ct, but the effects on different traits were not often proportional and they were highly variable in the particular years (range 10-69%) and cultivars (range < 0-60%). While the application of fungicide caused a reduction in DON content in all cultivars, an increase in pathogen content after the application of fungicides was not exceptional. The low fungicide effect on a reduction in pathogen content was connected with higher temperatures (temperature extremes) in a 30-day period of disease development. The efficacy of fungicide treatment for DON was low at high pathogen content and late heading. The use of the collected data to improve control measures is discussed. Keywords: i>, Fusarium culmorum, deoxynivalenol content, pathogen DNA content, real-time PCR assay, disease severity traits, disease control, cultivar resistance Journal: Czech Journal of Genetics and Plant Breeding Pages: 16-31 Volume: 43 Issue: 1 Year: 2007 DOI: 10.17221/1905-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/1905-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200701-0003.txt Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:1:id:1905-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: R. Šafaříková Title: List of field crop varieties registered in the Czech Republic in 2006 Journal: Czech Journal of Genetics and Plant Breeding Pages: 32 Volume: 43 Issue: 1 Year: 2007 DOI: 10.17221/1906-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/1906-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:1:id:1906-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Macháčková Title: Winter oilseed rape Aplaus Journal: Czech Journal of Genetics and Plant Breeding Pages: 33-34 Volume: 43 Issue: 1 Year: 2007 DOI: 10.17221/1902-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/1902-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:43:y:2007:i:1:id:1902-CJGPB