Template-Type: ReDIF-Article 1.0 Author-Name: Alena Nováková Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Kateřina Šimáčková Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Jan Bárta Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Vladislav Čurn Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Title: Potato variety identification by molecular markers based on retrotransposon analyses Abstract: We analyzed a set of twenty most grown potato (Solanum tuberosum L.) varieties listed in the Czech Variety List using the PCR-IRAP (Inter-Retrotransposon Amplified Polymorphism) method in order to distinguish fast and unambiguously the varieties. In total, 62 polymorphic alleles were amplified using the three primers P-Tst-1, P-Tst-3 and P-Tst-6. The recorded pattern of markers was stable and reproducible. The analyses were repeated three times and identical results were always obtained. The level of polymorphism varied from 11% to 79% depending on the respective primer. All analysed varieties could be reliably distinguished after multivariate statistics have been applied to the data obtained by the PCO and UPGMA analyses. The best resolution of individual varieties was obtained if all three primers were evaluated as a complex. The use of retrotransposon-based markers appears to be suitable for the differentiation of large sets of potato samples and should be an eligible complement to other molecular markers used in potato variety identification such as Simple Sequence Repeats (SSR) and Amplified Fragment Length Polymorphisms (AFLP). Keywords: molecular markers, PCR- IRAP, Solanum tuberosum, variety identification Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-10 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/11/2008-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/11/2008-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200901-0001.txt Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:11-2008-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Klaudia Jomová Author-Workplace-Name: Constantine the Philosopher University, Nitra, Slovakia Author-Name: Michaela Benková Author-Workplace-Name: Research Institute of Plant Production, Piešťany, Slovakia Author-Name: Ján Kraic Author-Workplace-Name: Constantine the Philosopher University, Nitra, Slovakia Author-Workplace-Name: Research Institute of Plant Production, Piešťany, Slovakia Title: Enrichment of chickpea genetic resources collection monitored by microsatellites Abstract: A set of 49 chickpea accessions maintained in the collection of the Genebank of the Slovak Republic was analyzed using microsatellite markers. The level of genetic diversity and the effectivity of new germplasm acquisition for the collection enrichment were evaluated. Five primer pairs used generated 50 different amplified alleles. Four of them, TA2, TA5, TR1, and TR7, containing a long TAA-tandem repeat, were polymorphic with 11-13 alleles per locus. A single fragment was obtained from all the accessions with the primer pair CATPER flanking a small microsatellite repetition in the mRNA sequence of chickpea cationic peroxidase. The genetic diversity was expressed as a diversity index (DI) and polymorphic information content (PIC) with values in the range from 0.885 to 0.904 and from 0.972 to 0.991, respectively. Recently acquired new germplasm contributed 13 new alleles to the entire collection, i.e. almost 70% of microsatellite diversity originated from the recently acquired accessions. Remarkable is also, that one accession carries a unique allel, not present in the remaining collection. Microsatellite analysis revealed the importance of collecting missions for the enrichment of collections of genetic resources. Keywords: chickpea, Cicer arietinum L., microsatellite, germplasm, acquisition Journal: Czech Journal of Genetics and Plant Breeding Pages: 11-17 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/49/2008-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/49/2008-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200901-0002.txt Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:49-2008-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Godfrey Akpan IWO Author-Workplace-Name: Department of Crop Science, University of Calabar, Calabar, Nigeria Author-Name: P.E. OLORUNJU Author-Workplace-Name: Department of Crop Science, University of Calabar, Calabar, Nigeria Title: Yield stability and resistance to leaf spot diseases and rosette in groundnut Abstract: Twenty-three advanced groundnut lines were evaluated for yield and resistance to early leaf spot (Mycosphaerella arachidis Deighton), late leaf spot (Mycosphaerella berkeleyi W.A. Jenkins) and rosette virus in on-station trials in 2001 and 2002. All the early groundnut lines were relatively resistant to rosette virus, early leaf spot and late leaf spot except ICGV-SM-93523 and ICGV-SM-93525, which were susceptible to late leaf spot. The medium maturing lines showed mostly higher levels of diseases, except MS16-791, which performed very well against all three diseases. The late groundnut lines were mostly susceptible to one or more of the diseases, except 49-85A and ICGV-SM-93532. Nine groundnut lines, combining high yield and resistance against all three diseases, were selected for on-farm trials at four locations in 2004 and 2005. In the on-farm trials all the nine selected lines were also resistant or highly resistant to rosette and both early and late leaf spot, while the local check, Makodi, was susceptible to all three diseases in both the on-station and on-farm trials. The analysis of yield data obtained at the eight environments, based on the linear statistical model yij = μ + ai+ ej + rij, estimated the genetic variance about three times higher than the residual variance. Still better results were obtained with the multiplicative model yij = μaibj+ ej + rij, where the genetic variance was more than four times higher than the residual variance and a still better differentiation of cultivars was thus possible. Significant differences in cultivar stability, expressed as the variation coefficient of the ai estimates (i.e. the variation of Standardised Relative Yields) from the multiplicative model across the eight environments, were observed. The check cultivar Makodi, though quite stable, was the lowest yielding of all tested ones. The groundnut line ICGV-1S-96805 combined very high yielding capacity and outstanding disease resistance with good, though not the highest, yield stability. Keywords: Arachis hypogea L., yielding capacity, multiplicative model, leaf spot, rosette virus, selection Journal: Czech Journal of Genetics and Plant Breeding Pages: 18-25 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/30/2008-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/30/2008-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200901-0003.txt Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:30-2008-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mohammad Anwar KHAN Author-Workplace-Name: Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, India Author-Name: Ram Gopal SAINI Author-Workplace-Name: Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, India Title: Non-hypersensitive leaf rust resistance of bread wheat cultivar PBW65 conditioned by genes different fromLr34 Abstract: : The bread wheat (Triticum aestivum L.) cultivar PBW65 has shown hight levels of resistance to the most frequent and highly virulent Indian race 77-5 of leaf rust (Puccinia triticina). The infection type and disease severity indicated a non-hypersensitive type of resistance against the race 77-5 in PBW65. The cultivar PBW65 was crossed with the leaf rust susceptible cultivar WL711 to determine the mode of inheritance of the resistance. The segregation for resistant and susceptible plants in the F2 and F3 generations revealed, that two genes, each showing additive effects, were likely to confer resistance to leaf rust in PBW65. Intercrossing of PBW65 with Cook (Lr34), RL6058 (Lr34) and HD2009, possessing a similar resistance level like PBW65, revealed that the genes for leaf rust resistance in PBW65 were non-allelic to Cook (Lr34), RL6058 (Lr34) as well as to the gene(s) in HD2009. It is concluded that the cultivar PBW65 is a novel source of non-hypersensitive leaf rust resistance. Keywords: Puccinia triticina, allelic test, durable resistance, Triticum aestivum L Journal: Czech Journal of Genetics and Plant Breeding Pages: 26-30 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/51/2008-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/51/2008-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-200901-0004.txt Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:51-2008-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Smýkal Title: IV International conference on legume genomics and genetics. Puerto Vallarta, Mexico, December 7-12, 2008 Journal: Czech Journal of Genetics and Plant Breeding Pages: 31-34 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/2661-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/2661-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:2661-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: V. Vrbovský Title: Poppy variety Orfeus Journal: Czech Journal of Genetics and Plant Breeding Pages: 35-36 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/3/2009-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3/2009-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:3-2009-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: L. Kameníková Author-Name: V. Vrbovský Title: White-seeded poppy varieties Orel and Racek Journal: Czech Journal of Genetics and Plant Breeding Pages: 37-38 Volume: 45 Issue: 1 Year: 2009 DOI: 10.17221/5/2009-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/5/2009-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:45:y:2009:i:1:id:5-2009-CJGPB