Template-Type: ReDIF-Article 1.0 Author-Name: Ritu MAHAJAN Author-Workplace-Name: School of Biotechnology, Jammu University, Jammu, India Author-Name: Priyanka GUPTA Author-Workplace-Name: National Bureau of Animal Genetic Resources, Karnal, India Title: Proteomics: taking over where genomics leaves off Abstract: The proteomic studies are simultaneously developed in several directions and significantly influence our notions on the capabilities of biological sciences. The need for proteomics research is necessary as there are certain genes in a cell that encode proteins with specific functions. Using a variety of techniques, proteomics can be used to study how proteins interact within a system or how the protein expression changes in different parts of the body, in different stages of its life cycle and in different environmental conditions as every individual has one genome and many proteomes. Besides the qualitative and quantitative description of the expressed proteins, proteomics also deals with the analysis of mutual interactions of proteins. Thereby, candidate proteins can be identified which may be used as starting-points for diagnostic or even therapeutic approaches. Keywords: applications, methods, protein databases, proteome, proteomics Journal: Czech Journal of Genetics and Plant Breeding Pages: 47-53 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/34/2009-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/34/2009-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201002-0001.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:34-2009-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Rhouma SAYAR Author-Workplace-Name: Kef Research Station, National Agriculture Research Institute of Tunisia (INRAT), Le Kef, Tunisia Author-Name: Houcine BCHINI Author-Workplace-Name: Physiology Laboratory, National Agriculture Research Institute of Tunisia (INRAT), Ariana, Tunisia Author-Name: Mohamed MOSBAHI Author-Workplace-Name: Kef Research Station, National Agriculture Research Institute of Tunisia (INRAT), Le Kef, Tunisia Author-Name: Habib KHEMIRA Author-Workplace-Name: Faculty of Sciences of Gabès, University of Gabès, Zrig Gabès, Tunisia Title: Response of durum wheat (Triticum durum Desf.) growth to salt and drought stresses Abstract: Two durum wheat (Triticum durum Desf.) cultivars were tested for salt and drought tolerance at germination, seedling emergence and early seedling growth in NaCl and PEG-8000 solutions of different osmotic potentials (-0.2, -0.4, -0.6 and -0.8 MPa). Daily and final germination and emergence percentage, as well as germination and seedling emergence rate, seedling growth, fresh and dry weight were recorded under controlled conditions. Results showed that germination and emergence rates were delayed by both solutions in both cultivars, but Omrabia showed higher germination and emergence rates than BD290273 in NaCl while BD290273 was less affected by NaCl and PEG solutions at the emergence stage. Sodium chloride had a lesser effect on both cultivars in terms of germination rate, emergence rate, final germination and emergence percentage than did PEG-8000. This conclusively proves that the adverse effect of PEG-8000 on germination, emergence and early seedling growth was due to the osmotic effect rather than to the specific ion. Seedling growth was reduced by both stresses. However, NaCl usually caused less damage than PEG to durum wheat seedlings, suggesting that NaCl and PEG acted through different mechanisms. Keywords: durum wheat, emergence rate, germination rate, salt and drought stresses, seedling growth Journal: Czech Journal of Genetics and Plant Breeding Pages: 54-63 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/85/2009-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/85/2009-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201002-0002.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:85-2009-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ajit Ghosh Author-Workplace-Name: Plant Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh Author-Name: Sazia Sharmin Author-Workplace-Name: Plant Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh Author-Name: Sazzadul Islam Author-Workplace-Name: Plant Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh Author-Name: Minhaz Uddin Pahloan Author-Workplace-Name: Plant Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh Author-Name: Shahidul Islam Author-Workplace-Name: Bangladesh Jute Research Institute, Dhaka, Bangladesh Author-Name: Haseena Khan Author-Workplace-Name: Plant Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh Title: SSR markers linked to mite (Polyphagotarsonemus latus Banks) resistance in jute (Corchorus olitorius L.) Abstract: The mite is considered as one of the major limiting factors of jute production in Bangladesh. So it is essential to select genotypes with the desired resistance allele combination using DNA markers. Eighty-eight SSR primers were used to screen the mite resistant and sensitive parents. Among them only twenty-one primer pairs showed polymorphism. A genetic linkage map was constructed using these polymorphic primers in one hundred and fifty F2 populations, derived from a cross between the parents. The SSR markers J-170 and HK-89 have been mapped at 34.1 cM and 35.4 cM, respectively, on either side of the phenotypic marker. These two markers (J-170 and HK-89) along with HK-64 showed 100% selection efficiency in combination. Keywords: jute, linkage map, mite, SSR Journal: Czech Journal of Genetics and Plant Breeding Pages: 64-74 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/71/2009-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/71/2009-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201002-0003.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:71-2009-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Samira SALAYEVA Author-Workplace-Name: Genetic Resources Institute of ANAS, Baku, Azerbaijan Author-Name: Ellada AKHUNDOVA Author-Workplace-Name: Department of Genetics and Darwinism, Faculty of Biology, Baku State University, Azerbaijan Author-Name: Alamdar MAMMADOV Author-Workplace-Name: Institute of Botany of ANAS, Baku, Azerbaijan Title: Evaluation of DNA polymorphism among cultivated and wild grapevine accessions from Azerbaijan Abstract: To estimate genetic relationships among 31 cultivated and 34 wild grape accessions originating from regions near the Caspian Sea in the Azerbaijan Republic, RAPD analysis was performed with 27 decamer primers selected from a total of 55 primers. The most discriminating primers were OPC-16, OPF-18 and OPA-17, which showed the highest values of genetic diversity (0.927, 0.914 and 0.909, respectively). The lowest values of diversity pertained to the markers OPA-1 (0.615) and V-20 (0.624). The cluster analysis representing genetic similarity among all selected samples divided the genotypes into nine separate groups at similarity index 0.508. Within the studied Azerbaijan grape populations the highest genetic diversity belonged to the population of cultivated samples originating from the Absheron peninsula, with a diversity index 0.852 and the next ranks were assigned to the wild populations originating from Nabran and Guba regions, with a diversity index 0.824 and 0.793, respectively. The lowest diversity was observed within Davachi individuals, with a diversity index 0.765. The wild population from Azerbaijan was molecularly similar to the cultivated gene pool from this area. This result supported the hypothesis that the southwest of the Caspian Sea is a region where grape was brought into culture. During the analysis a special band was observed which could be used in identifying wild and cultivated grape accessions with high or low resveratrol content. The results of this work clearly indicated that the RAPD analysis can be used to estimate genotypic similarities, genetic diversity and for clustering cultivated and wild grape accessions. Keywords: Keywords: Azerbaijan wild and cultivated grape, genetic diversity, RAPD markers, resveratrol, similarity index Journal: Czech Journal of Genetics and Plant Breeding Pages: 75-84 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/12/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/12/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201002-0004.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:12-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Taťána SUMÍKOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Alena HANZALOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat Abstract: Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered in the Czech Republic. Gene Lr37 was detected in four tested cultivars (Bakfis, Biscay, Nicol, Mulan), gene Lr26 occurred only in one cultivar (Etela) and three cultivars (Clarus, Orlando and Rapsodia) were found to carry both these genes. Data obtained by PCR markers were compared with results of greenhouse and field tests. Seedling reactions of cultivars possessing Lr26 to seven different leaf rust isolates conformed to the results obtained by the marker analysis, however, there were found some discrepancies in the detections of Lr37, which could be detected in greenhouse seedling tests only with difficulties. Keywords: leaf rust, Lr26, Lr37, multiplex PCR, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 85-89 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/32/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/32/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201002-0005.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:32-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Bartoš Title: Rusts epidemics and their implications in wheat breeding and research in the Czech Republic Journal: Czech Journal of Genetics and Plant Breeding Pages: 90-96 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/52/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/52/2010-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:52-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Laml Author-Name: J. Pánek Title: Winter wheat Federer Journal: Czech Journal of Genetics and Plant Breeding Pages: 97-98 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/54/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/54/2010-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:54-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Horčička Author-Name: O. Veškrna Author-Name: T. Sedláček Author-Name: J. Chrpová Title: Winter wheat Secese Journal: Czech Journal of Genetics and Plant Breeding Pages: 99-101 Volume: 46 Issue: 2 Year: 2010 DOI: 10.17221/55/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/55/2010-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:2:id:55-2010-CJGPB