Template-Type: ReDIF-Article 1.0 Author-Name: Fethi Bnejdi Author-Workplace-Name: Laboratoire de Génétique et Biométrie, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Tunis, Tunisia Author-Name: Mourad Saadoun Author-Workplace-Name: Institut National de la Recherche Agronomique de Tunisie (INRAT), Tunisia Author-Name: Mohamed El Gazzah Author-Workplace-Name: Laboratoire de Génétique et Biométrie, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, Tunis, Tunisia Title: Cytoplasmic effects on grain resistance to yellowberry in durum wheat Abstract: Parental, F1, reciprocal F1 (RF1), F2, reciprocal F2 (RF2), BC1P1 and BC1P2 generations of four crosses involving four cultivars of durum wheat (Triticum durum Desf.) were evaluated for grain resistance to yellowberry. Significant differences were reported for F1, F2 and their reciprocals in all crosses. A generation means analysis indicated the inadequacy of additive-dominance model and additive-dominance model considering maternal effects. However, the variation in generation means in the four crosses could be explained by a digenic epistatic model with cytoplasmic effects. Cytoplasmic effects were significant and consistent in all the crosses. Dominance effects and additive × dominance epistasis were more important than additive effects and other epistatic components. The choice of a female parent possessing grain resistance to yellowberry appeared to be decisive in durum wheat breeding for resistance to this serious seed disorder. Keywords: cytoplasmic effects, resistance to yellowberry, Triticum durum Journal: Czech Journal of Genetics and Plant Breeding Pages: 145-148 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/18/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/18/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201004-0001.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:18-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ludmila Holková Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Name: Pavlína Mikulková Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Name: Pavlína Hrstková Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Name: Ilja Tom Prášil Author-Workplace-Name: Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Marta Bradáčová Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Name: Pavla Prášilová Author-Workplace-Name: Department of Genetics and Plant Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Oldřich Chloupek Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Title: Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley Abstract: The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from -14.2°C to -15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT50 from -10.0°C to -14.3°C). The genetic linkage between Dhn4 and Dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F2 plants from a cross between two genotypes carrying different allelic combination of Dhn4 and Dhn7 genes (two-rowed spring variety Akcent × six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant. Keywords: abiotic stress, dehydrins, gene polymorphism, Hordeum vulgare Journal: Czech Journal of Genetics and Plant Breeding Pages: 149-158 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/90/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/90/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201004-0002.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:90-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tibor Sedláček Author-Workplace-Name: Plant Breeding Station Stupice, Research Centre SELTON, Sibřina, Czech Republic Author-Name: Pavel Mařík Author-Workplace-Name: Plant Breeding Station Stupice, Research Centre SELTON, Sibřina, Czech Republic Author-Name: Jana Chrpová Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Development of CAPS marker for identifi cation of rym4 and rym5 alleles conferring resistance to the Barley Yellow Mosaic Virus complex in barley Abstract: In barley, soil-borne viruses of the barley mosaic virus complex (BaYMV, BaMMV, BaYMV-2) are of high importance due to their increased spread, yield losses, and the fact that protection with chemicals is not possible. Concerning resistance breeding, simple, cheap, and robust selection methods are required. Markerassisted selection (MAS) has a great potential to meet this demand. A CAPS marker designed directly for SNPs causing mutations responsible for resistance of rym4/rym5 alleles of the Hv-eIF4E gene was developed in this study. Results showed perfect correspondence between the CAPS marker and the allele identified indirectly on the basis of field tests. Keywords: barley, marker-assisted breeding, mosaic virus complex, resistance, rym allele identification Journal: Czech Journal of Genetics and Plant Breeding Pages: 159-163 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/7/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/7/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201004-0003.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:7-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Sudhir Shukla Author-Workplace-Name: Genetics and Plant Breeding, National Botanical Research Institute, Rana Pratap Marg, Lucknow, India Author-Name: Hemant Kumar Yadav Author-Workplace-Name: Genetics and Plant Breeding, National Botanical Research Institute, Rana Pratap Marg, Lucknow, India Author-Name: Anu Rastogi Author-Workplace-Name: Genetics and Plant Breeding, National Botanical Research Institute, Rana Pratap Marg, Lucknow, India Author-Name: Brij Kishore Mishra Author-Workplace-Name: Genetics and Plant Breeding, National Botanical Research Institute, Rana Pratap Marg, Lucknow, India Author-Name: Sant Prasad Singh Author-Workplace-Name: Genetics and Plant Breeding, National Botanical Research Institute, Rana Pratap Marg, Lucknow, India Title: Alkaloid diversity in relation to breeding for specifi c alkaloids in opium poppy (Papaver somniferum L.) Abstract: Papaver somniferum is a chief source of diverse physiologically active alkaloids, required by the pharmaceutical industry. The present study describes the diversity of the alkaloid spectrum of 122 opium poppy accessions of Indian origin by means of a cluster analysis based on Mahalanobis generalised distances. The accessions could be grouped into 11 clusters according to their relationship between the contents of morphine, codeine, thebaine, narcotine and papaverine in raw opium. The diversity of the alkaloid spectrum of 11 clusters reflected the very low correlations between the contents of the individual alkaloids across the 122 entries, found earlier. The clusters represented almost all possible combinations of the high content of an alkaloid with high or low content of another alkaloid. Although on average the morphine content exceeds the sum of the other four alkaloids, in one cluster the narcotine content (15.3%) was even higher than that of morphine (14.6%) and the content of the remaining alkaloids was also extremely high. The variation range among the clusters was for papaverine between 0.14% to 5.3%, while for morphine between 12.4% to 18.0%. The results indicate a large space for the breeding of opium poppy for individual alkaloids or particular combinations of alkaloids, as required by pharmaceutical industries. Keywords: alkaloids, clustering, multivariate, Papaver somniferum Journal: Czech Journal of Genetics and Plant Breeding Pages: 164-169 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/96/2009-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/96/2009-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201004-0004.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:96-2009-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Aydin Uzun Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri, Turkey Author-Name: Osman Gulsen Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri, Turkey Author-Name: Turgut Yesiloglu Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, Turkey Author-Name: Yildiz Aka-Kacar Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, Turkey Author-Name: Onder Tuzcu Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, Turkey Title: Distinguishing grapefruit and pummelo accessions using ISSR markers Abstract: Grapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus paradisi Macf.), five pummelo (Citrus maxima (Burm.) Merr.) and one Citrus hassaku Hort. Ex Tanaka accessions. Twelve ISSR primers produced a total of 100 fragments and 62 of them were polymorphic. The number of average polymorphic fragments per primer was 5.2. The mean polymorphism information content (PIC) was 0.37. The unweighted pair group method arithmetic average (UPGMA) analysis demonstrated that the accessions had a similarity range from 0.79 to 1.00. The accessions were separated into two main clusters; group A with five pummelos and group B with grapefruits. In the pummelo cluster, all pummelos were distinguished whereas in the grapefruit cluster some accessions were not clearly separated. There was a low level of variation in the grapefruits due to their mutation origin. Keywords: accession, Citrus maxima, Citrus paradisi, germplasm characterization, molecular markers Journal: Czech Journal of Genetics and Plant Breeding Pages: 170-177 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/89/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/89/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201004-0005.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:89-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Sebastien Gadiou Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague, Czech Republic Author-Name: Jiban Kumar Kundu Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague, Czech Republic Title: Complete genome sequence of a Brome Mosaic Virus isolate from the Czech Republic Abstract: An isolate of Brome mosaic virus (BMV) was originally isolated from Agropyron repens and maintained in Hordeum vulgare. The full-length genome of this isolate (BMV-CZ) was sequenced. Phylogenetic analysis revealed that BMV-CZ shared a minimum of 95.6% sequence identity, localized in the 5'-UTR of RNA-1 with the other BMV isolates from the database, and a maximum of divergence of 30.8% with Broad bean mottle virus localized in the 5'-UTR of RNA-3. This is the first sequence report of full-length BMV from the Czech Republic. Keywords: BMV, sequence analysis, variability Journal: Czech Journal of Genetics and Plant Breeding Pages: 178-182 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/60/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/60/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201004-0006.txt Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:60-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Horčička Author-Name: O. Veškrna Author-Name: T. Sedláček Author-Name: J. Chrpová Title: Winter wheat Elly Journal: Czech Journal of Genetics and Plant Breeding Pages: 183-185 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/125/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/125/2010-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:125-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I. Hasalová Author-Name: R. Trojan Author-Name: R. Dostálová Title: Green pea variety Morris Journal: Czech Journal of Genetics and Plant Breeding Pages: 186-187 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/124/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/124/2010-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:124-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 46 Journal: Czech Journal of Genetics and Plant Breeding Pages: X1 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/6814-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6814-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:46:y:2010:i:4:id:6814-CJGPB