Template-Type: ReDIF-Article 1.0 Author-Name: Nelli A. Hovhannisyan Author-Workplace-Name: Department of Ecology and Nature Protection, Faculty of Biology, Yerevan State University, Yerevan, Armenia Author-Name: Mohammad Ehsan Dulloo Author-Workplace-Name: Bioversity International, Rome, Italy Author-Name: Aleksandr H. Yesayan Author-Workplace-Name: Department of Ecology and Nature Protection, Faculty of Biology, Yerevan State University, Yerevan, Armenia Author-Name: Helmut Knüpffer Author-Workplace-Name: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany Author-Name: Ahmed Amri Author-Workplace-Name: International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria Title: Tracking of powdery mildew and leaf rust resistance genes in Triticum boeoticum and T. urartu, wild relatives of common wheat Abstract: Wild Triticum and Aegilops species are increasingly used in wheat breeding programmes around the world as donors of genes conferring resistance to biotic and abiotic stresses, as well as of genes that contribute to the improvement of grain quality. In the present study, thirty-nine accessions of diploid species with the A genome (Triticum boeoticum and T. urartu) were evaluated for the presence of the genes conferring resistance to powdery mildew (Blumeria graminis) and leaf rust (Puccinia recondita) using both inoculation tests and sequence tagged sites (STS) marker analyses in order to find correspondence between STS markers and resistance as a trait. The most resistant entries were T. boeoticum accessions. All the marked Lr and Pm resistance genes (Pm1, Pm2, Pm3, Lr10, Lr47, Lr25 and Lr28) were identified in the check T. aestivum cultivar Bezostaya 1. The resistance to powdery mildew in the material studied was conferred by the combination of the Pm1 gene with either Pm2 or Pm3. The Pm1 and Pm3 markers appeared to be suitable for tracking these powdery mildew resistance genes, while the Pm2 gene marker cannot be considered as usable in various genetically different wheat accessions. The presence of the genes Lr25, Lr28 and Lr47 seems to be particularly useful for obtaining leaf rust resistance in T. boeoticum and T. urartu species. Keywords: Blumeria graminis, inoculation test, Puccinia recondita, resistance genes, STS marker, Triticum boeo-ticum, Triticum urartu Journal: Czech Journal of Genetics and Plant Breeding Pages: 45-57 Volume: 47 Issue: 2 Year: 2011 DOI: 10.17221/127/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/127/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201102-0001.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:127-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jana Chrpová Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Václav Šíp Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Lenka Štočková Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Lenka STEMBERKOVÁ Author-Workplace-Name: Research Centre SELTON, Ltd., Breeding Station Stupice, Czech Republic Author-Name: Ludvík TVARŮŽEK Author-Workplace-Name: Agrotest Fyto, Ltd., Kroměříž, Czech Republic Title: Resistance to Fusarium head blight in spring barley Abstract: Fusarium head blight (FHB) is a fungal disease causing substantial yield and quality losses in barley. Genetic variation in deoxynivalenol (DON) content and and important yield traits in response to FHB were studied in 44 spring barley cultivars for two years following artificial inoculation with Fusarium culmorum under field conditions. The analysis of variance revealed that the largest effect on DON content and simultaneously on the reduction of thousand grain weight and grain weight per spike were due to the environmental conditions of the year, while the visual disease symptoms depended on the cultivars to a larger extent. All these traits were significantly interrelated. The most resistant cultivars Murasski mochi, Nordic, Krasnodarskij 35, Krasnodarskij 95, Nordus, and Usurijskij 8, together with the resistant check Chevron, showed the lowest DON content, the lowest expression of disease symptoms and the lowest reduction of TGW and GWS. However, most spring barley cultivars registered in the Czech Republic in recent years expressed susceptibility or medium resistance and were considerably affected by the disease. This increases the importance of breeding barley for resistance to FHB. Keywords: cultivar resistance, Fusarium culmorum, head blight, Hordeum vulgare, mycotoxin DON Journal: Czech Journal of Genetics and Plant Breeding Pages: 58-63 Volume: 47 Issue: 2 Year: 2011 DOI: 10.17221/129/2010-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/129/2010-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201102-0002.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:129-2010-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Antonín DREISEITL Author-Workplace-Name: Agrotest Fyto Ltd., Kroměříž, Czech Republic Title: Presence of the newly designated powdery mildew resistance Landi in some winter barley cultivars Abstract: In the last two decades, resistance to the powdery mildew pathogen has been investigated in a large number of barleys in the Czech Republic. Several tens of winter barley cultivars were identified with a resistance based on an unknown gene or unknown combinations of resistance genes. In this paper tests on 20 of these cultivars are presented. Thirty-two reference isolates of Blumeria graminis f.sp. hordei were used. All the 20 cultivars shared a particular, previously unknown resistance. Landi was the first cultivar registered with this resistance and it is recommended that this resistance be designated Ln. Isolates virulent to Ln were already found randomly in old European, and also in non-European pathogen populations, where cultivars possessing the resistance Ln were never grown. On the other hand, the resistance Ln has been highly effective even 13 years after Landi registration. Keywords: Blumeria graminis f.sp. hordei, Hordeum vulgare, pathogen isolates, reaction types Journal: Czech Journal of Genetics and Plant Breeding Pages: 64-68 Volume: 47 Issue: 2 Year: 2011 DOI: 10.17221/14/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/14/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201102-0003.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:14-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tian Li Author-Workplace-Name: Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China Author-Name: Jing Kuan Sun Author-Workplace-Name: Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China Author-Name: Zhao Hua Lu Author-Workplace-Name: Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China Author-Name: Qing Liu Author-Workplace-Name: Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China Title: Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens Abstract: The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern analysis of the plants were positive. It was for the first time shown that the HBsAg (hepatitis B surface antigen) gene was introduced into tomato plants. The expression of transgene is under investigation. Keywords: Agrobacterium, HBsAg gene, tomato, transformation Journal: Czech Journal of Genetics and Plant Breeding Pages: 69-77 Volume: 47 Issue: 2 Year: 2011 DOI: 10.17221/5/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/5/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201102-0004.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:5-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Horčička Author-Name: O. Veškrna Author-Name: T. Sedláček Author-Name: J. Chrpová Title: Winter wheat Matylda Journal: Czech Journal of Genetics and Plant Breeding Pages: 78-80 Volume: 47 Issue: 2 Year: 2011 DOI: 10.17221/46/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/46/2011-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:46-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Mařík Title: Winter barley Lester Journal: Czech Journal of Genetics and Plant Breeding Pages: 81-83 Volume: 47 Issue: 2 Year: 2011 DOI: 10.17221/52/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/52/2011-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:2:id:52-2011-CJGPB