Template-Type: ReDIF-Article 1.0 Author-Name: Lutfor Rahman Author-Workplace-Name: Department of Plant Science and Research, Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea Author-Name: Sakina Khanam Author-Workplace-Name: Department of Plant Science and Research, Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea Author-Workplace-Name: Division of Crop Physiology, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh Author-Name: Jae-Hwan Roh Author-Workplace-Name: Rice Research Division, National Institute of Crop Science, Rural Development Administration, Suwon, Korea Author-Name: Hee-Jong Koh Author-Workplace-Name: Department of Plant Science and Research, Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea Title: Mapping of QTLs involved in resistence to rice blast (Magnaporthe grisea) using Oryza minuta introgression lines Abstract: Introgression lines derived from Oryza minuta and O. sativa subsp. japonica var. Junambyeo were crossed for a mapping of the population composed of 112 recombinant lines to identify putative QTLs against rice blast disease using the percentage of diseased leaf area. By using 148 Sequence Tagged Site (STS) and Single Sequence Repeat (SSR) markers, five QTLs on chromosomes 6, 7, 9 and 11 and seven epistatic QTLs were identified against two blast isolates (KI307 and KI209). Of them two QTLs (qKI307-2 and qKI209-3) shared a similar position on chromosome 11. O. minuta introgression contributed the resistance allele for all of these QTLs. Combined phenotypic variations by QTL and (E-QTL) accounted for 56.9% against KI307, and 53.4% against KI209. Each QTL could account for the resistance variation between 11 and 24.6%. The resistance from wild introgressions was attributable to a combination of QTLs and epistatic effects between different loci, capable of inducing hypersensitive reactions. Our findings are in support of the strategy of pyramiding major QTLs to develop improved rice varieties with durable broad spectrum resistance against the blast fungus. Keywords: blast, introgression from Oryza minuta, quantitative trait locus (QTL), rice Journal: Czech Journal of Genetics and Plant Breeding Pages: 85-94 Volume: 47 Issue: 3 Year: 2011 DOI: 10.17221/19/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/19/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201103-0001.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:3:id:19-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Antonín DREISEITL Author-Workplace-Name: Agrotest Fyto Ltd., Kroměříž, Czech Republic Title: Dissimilarity of barley powdery mildew resistances Lomerit and Heils Hanna Abstract: The resistance Heils Hanna (HH) was postulated in several tens of 471 previously tested winter barley cultivars. In this paper, new tests on 29 of these cultivars are reported. Thirty-two reference isolates of Blumeria graminis f.sp. hordei held in the pathogen genebank at the Agricultural Research Institute in Kromeriz, Ltd. including a Japanese isolate and five Israeli isolates were used for response tests. However, the resistance HH conferred by the gene Mla8 and herein characterised by reaction type 0 to an old Japanese isolate known as Race I was now postulated only in four cultivars. In the other 25 cultivars another resistance, characterised by reaction type 0 to Race I and also to two Israeli isolates, was detected. In addition to the two mentioned resistances, eight known (Bw, Dr2, Ha, IM9, Ln, Lv, Ra and Sp) resistances were found in the set examined. Lomerit was the only registered cultivar tested here in which the newly detected resistance was present alone, therefore, it is recommended that this resistance be designated Lo. Keywords: Blumeria graminis f.sp. hordei, Hordeum vulgare, pathogen isolates, reaction types, resistance gene postulation, pedigree analysis Journal: Czech Journal of Genetics and Plant Breeding Pages: 95-100 Volume: 47 Issue: 3 Year: 2011 DOI: 10.17221/45/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/45/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201103-0002.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:3:id:45-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Guolin CHEN Author-Workplace-Name: School of Agriculture and Food Science, Zhejiang A&F University, Linan, China Author-Workplace-Name: Department of Agronomy, Zhejiang University, Hangzhou, China Author-Name: WU Jianguo Author-Workplace-Name: Department of Agronomy, Zhejiang University, Hangzhou, China Author-Name: Chunhai SHI Author-Workplace-Name: Department of Agronomy, Zhejiang University, Hangzhou, China Title: Dynamic genetic effects on threonine content in rapeseed (Brassica napus L.) meal at different developmental stages Abstract: Dynamic genetic effects on threonine content (TC) of rapeseed (Brassica napus L.) meal were analysed at 5 developmental times/stages using the genetic models for diploid plant seeds. Results indicated that the expression of diploid embryo, cytoplasmic and diploid maternal plant genes were all important for the performance of TC at various developmental times/stages of rapeseed, especially at the early and middle developmental stages. Among different genetic systems, TC was mainly controlled by the cumulative or net maternal main effects and the genotype × environment (GE) interaction effects, followed by the embryo main effects and GE interaction effects. The expression of genes was more easily influenced by the environmental factors at the first three developmental stages. The total narrow-sense heritabilities for TC on 15, 22, 29, 36 and 43 days post anthesis were 46.50, 62.60, 57.10, 84.70 and 59.50%, respectively, of which the interaction heritabilities were more important at the first three developmental stages of rapeseed. The improvement in TC of rapeseed meal could be expected by selection based on the higher narrow-sense heritabilities near maturity. The predicted genetic effects of parents showed that TC of progeny could be improved by using the parent Gaoyou 605. Keywords: developmental genetics, genetic variances and conditional genetic variances, heritability, rapeseed meal, threonine content Journal: Czech Journal of Genetics and Plant Breeding Pages: 101-113 Volume: 47 Issue: 3 Year: 2011 DOI: 10.17221/17/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/17/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201103-0003.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:3:id:17-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Samad Habibzadeh ARDEBILI Author-Workplace-Name: Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Author-Workplace-Name: Department of Agronomy and Plant Breeding, Abooreihan Campus, University of Tehran, Pakdasht-Tehran, Iran Author-Name: Mehran E. SHARIATPANAHI Author-Workplace-Name: Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Author-Name: Reza AMIRI Author-Workplace-Name: Department of Agronomy and Plant Breeding, Abooreihan Campus, University of Tehran, Pakdasht-Tehran, Iran Author-Name: Mona EMAMIFAR Author-Workplace-Name: Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Author-Workplace-Name: Department of Plant Breeding, Sari University of Agricultural and Natural Resources, Sari, Iran Author-Name: Mahnaz OROOJLOO Author-Workplace-Name: Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Author-Name: Ghorbanali NEMATZADEH Author-Workplace-Name: Department of Plant Breeding, Sari University of Agricultural and Natural Resources, Sari, Iran Author-Name: Seyed Ahmad Sadat NOORI Author-Workplace-Name: Department of Agronomy and Plant Breeding, Abooreihan Campus, University of Tehran, Pakdasht-Tehran, Iran Author-Name: Erwin HEBERLE-BORS Author-Workplace-Name: Max F. Perutz Laboratories, Vienna University, Vienna, Austria Title: Effect of 2,4-D as a novel inducer of embryogenesis in microspores of Brassica napus L. Abstract: The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) applied at high concentrations for a short time was investigated as a novel stress for induction of microspore embryogenesis for the first time. Brassica napus L. cvs. Topas and Hyola 420 were used as model plants for testing this hypothesis. Microspores were subjected to 2,4-D at 4 concentrations (15, 25, 35 and 45 mg/l) for 15-45 min while the classical heat shock was used as the control treatment. Among 2,4-D treatments in Topas, the highest yield of torpedo-stage embryos was achieved at 15 mg/l 2,4-D for 30 min while more normal plantlets were produced when 2,4-D (25 mg/l for 30 min) was applied to the microspores. In Hyola 420 the results showed a lower number of embryos and normal plantlets at all concentrations of 2,4-D. Although Hyola 420 was almost equally embryogenic as Topas after heat shock treatment, large differences between genotypes (concerning embryogenic response) occurred after 2,4-D treatment. However, the mean number of embryos and regenerants was higher in heat shock as compared to 2,4-D induced stress (one magnitude of order). According to the results obtained, 2,4-D can be introduced as a new stress for induction of embryogenesis in microspores similarly like in zygotic and somatic cells. This novel stress is very important for plant species whose microspores are extremely sensitive to classical stresses. Keywords: 2, 4-D, Brassica napus, doubled haploids, microspore embryogenesis, rapeseed Journal: Czech Journal of Genetics and Plant Breeding Pages: 114-122 Volume: 47 Issue: 3 Year: 2011 DOI: 10.17221/4/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/4/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201103-0004.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:3:id:4-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tibor SEDLÁČEK Author-Workplace-Name: SELTON Research Centre, Sibřina, Czech Republic Author-Name: Pavel HORČIČKA Author-Workplace-Name: SELTON Research Centre, Sibřina, Czech Republic Title: Development of a small-scale variant of the Rapid Mix Test experimental bread baking Abstract: To determine wheat quality, an experimental 80 g variant (mini-RMT) of the standard 1050 g Rapid Mix Test (RMT) was developed. Ten samples of wheat flour with known RMT values were analysed using the method. Results showed a strong correlation between RMT and mini-RMT values (r = 0.96). Mini-RMT bread volumes were smaller on average by 47.4 ml. A regression equation was constructed allowing the mini-RMT to be used in place of the RMT in wheat breeder laboratories. Anyway, these results are of limited plausibility due to the limited sample numbers and have to be verified on a larger sample set. Keywords: experimental bread baking, quality, rapid mix test, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 123-127 Volume: 47 Issue: 3 Year: 2011 DOI: 10.17221/47/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/47/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201103-0005.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:3:id:47-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: E. Tejklová Author-Name: M. Bjelková Author-Name: M. Pavelek Title: Medium-linolenic linseed (Linum usitatissimum L.) Raciol Journal: Czech Journal of Genetics and Plant Breeding Pages: 128-130 Volume: 47 Issue: 3 Year: 2011 DOI: 10.17221/96/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/96/2011-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:3:id:96-2011-CJGPB