Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 47 Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/4357-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/4357-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:4357-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Václav ŠÍP Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jana CHRPOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Lenka ŠTOČKOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Evaluation of resistance to Fusarium head blight in wheat using different sources of inoculum Abstract: The response of four winter wheat cultivars, differing in resistance to Fusarium head blight (FHB), to spray inoculation with four selected Fusarium graminearum isolates, mixture of these isolates and frequently used F. culmorum isolate B was studied in five field and glasshouse experiments during 2008-2010. Analyses of variance showed highly significant main and interaction effects of cultivar, inoculum source and environment (year-trial) on all five examined traits indicative of disease severity, yield loss and accumulation of mycotoxins. The relations between traits were not evidently influenced by the used isolate. Resistance of host genotypes and environmental conditions accounted for a greater proportion of the total variation (8-36%) than the inoculation source (isolate) that substantially influenced the accumulation of the mycotoxin deoxynivalenol (12%), but expressed relatively low effects on symptom scores, percentage of fusarium damaged kernels and reductions of yield components (2-4%). Two-way and three-way interactions accounted for 25-40% of variation in the examined traits, which indicates great importance of multi-environment tests, using different Fusarium isolates for inoculation. Separate inoculation with F. graminearum isolates, differing in aggressiveness, did not appear to be more advantageous than their use in mixture that showed medium or below-average aggressiveness in all traits. The application of an isolate mixture could be recommended as a "less costly" alternative to inoculation with single isolates in trials repeated in different years and/or locations. It was indicated by these experiments that especially the detection of resistance/moderate resistance to FHB could be facilitated by the use of a carefully selected mixture of isolates. However, the application of aggressive isolates (isolate B of F. culmorum in these experiments) appeared to be beneficial to eliminate FHB susceptible materials in the breeding process. Keywords: artificial inoculation, breeding for resistance, DON content, FHB traits, Fusarium isolates, isolate mixture, winter wheat (Triticum aestivum L.) Journal: Czech Journal of Genetics and Plant Breeding Pages: 131-139 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/112/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/112/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201104-0002.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:112-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Niloofar Rostamkhani Author-Workplace-Name: Department of Agricultural Biotechnology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Author-Name: Ali Haghnazari Author-Workplace-Name: Department of Agricultural Biotechnology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Author-Name: Masoud Tohidfar Author-Workplace-Name: Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran Author-Name: Aboubakr Moradi Author-Workplace-Name: Department of Agricultural Biotechnology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Title: Rapid identification of transgenic cotton (Gossypium hirsutum L.) plants by loop-mediated isothermal amplification Abstract: In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was extracted from fresh leaf tissues of T2 transgenic cotton containing chitinase (chi) and cry1A(b) genes. Detection of genes of interest was performed by polymerase chain reaction (PCR), LAMP and real-time PCR methods. In LAMP assay the amplification was performed after 30 min at 65°C when loop primers were involved in the reaction. The involvement of loop primers decreased the time needed for amplification. By testing serial tenfold dilutions (10-1 to 10-8) of the genes of interest, the detection sensitivity of LAMP was found to be 100-fold higher than that of PCR. The rapid DNA extraction method and LAMP assay can be performed within 30 min and the derived LAMP products can be directly observed as visually detectable based on turbidity in the reaction tube. The accuracy of LAMP method in the screening of transgenes was confirmed by PCR and real-time PCR. The developed method was efficient, rapid and sensitive in the screening of cotton transgenic plants. This method can be applied to any other crops. Keywords: Bt, chitinase, CryIA(b), detection, loop-mediated isothermal amplification, transgenic cotton Journal: Czech Journal of Genetics and Plant Breeding Pages: 140-148 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/7/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/7/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201104-0003.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:7-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zhen HUANG Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Author-Name: Yu ZHANG Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Author-Name: Hui Qiang LI Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Author-Name: Li YANG Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Author-Name: Yuan Yuan BAN Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Author-Name: Ai Xia XU Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Author-Name: En Shi XIAO Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China Title: AFLP and SSR markers linked to the yellow seed colour gene in Brassica juncea L. Abstract: Yellow mustard, cultivated in northern Shaanxi of China, is a valuable germplasm of Brassica juncea with low erucic acid content. Its yellow seed colour is controlled by a recessive allele of a single gene, whose dominant allele conditions brown seed colour. To map the yellow seed colour allele, amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) technologies were used to identify markers linked to the recessive allele. The analysis was done on 386 F2 plants, segregating for seed colour, from the cross Wuqi yellow mustard × Wugong mustard. The plants were selfed to determine their seed colour genotype. Twenty AFLP markers and eight SSR markers were identified from 256 AFLP primer combinations and 624 pairs of SSR primers, respectively. Blast analysis indicated that the sequences of four closely linked AFLP and SSR markers showed good collinearity with those of Arabidopsis chromosome 3, and the homologue of the yellow seed colour allele was located between At3g14190 and At3g32130. Sequence information of the region between the two genes of Arabidopsis could be used to develop more closely linked markers to narrow down the homologue of the yellow seed colour allele. These results would accelerate the procedure of yellow seed colour gene cloning and marker-assisted selection for yellow mustard. Keywords: Brassica juncea L., comparative analysis, fine mapping, yellow seed colour allele Journal: Czech Journal of Genetics and Plant Breeding Pages: 149-155 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/109/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/109/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201104-0004.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:109-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Josef Patzak Author-Workplace-Name: Hop Research Institute, Žatec, Czech Republic Author-Name: František Paprštein Author-Workplace-Name: Research and Breeding Institute of Pomology in Holovousy, Holovousy, Hořice v Podkrkonoší, Czech Republic Author-Name: Alena Henychová Author-Workplace-Name: Hop Research Institute, Žatec, Czech Republic Title: Identification of apple scab and powdery mildew resistance genes in Czech apple (Malus × domestica) genetic resources by PCR molecular markers Abstract: The presence of genes for resistance to scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) was studied using molecular markers in a sample of 279 apple cultivars from the Czech collection of apple genetic resources. The sample comprised 37 cultivars supposed to have the Vf gene for scab resistance, 97 reference world cultivars and 145 old and local cultivars. Six PCR molecular markers for the scab resistance genes Vf, Vm, Vbj, Vr and Vh and three PCR molecular markers for the powdery mildew resistance genes Pl-w, Pl-1 and Pl-d were used. The marker for the major scab resistance gene Vf was detected in all cultivars supposed to have Vf, except in Romus 1, and in the three small-fruited cultivars Malus Evereste, Golden Gem and Hilleri. The markers of the Vr and Vh scab resistance genes were detected in 22 cultivars in combination with the marker for Vf, in 56 reference world cultivars and in 82 old and local apple cultivars. PCR molecular markers for one or two of the powdery mildew resistance genes were detected in the small-fruited cultivars Malus Evereste, Golden Gem, prof. Sprengeri and Hilleri; and in the larger fruited cultivars Hagloe Crab, Borovinka and Tita Zetei. We did not find markers for the scab resistance genes Vm and Vbj in any of the studied cultivars. They are absent also in the remaining part of the Czech collection of apple genetic resources. PCR molecular markers are useful tools for the identification of resistance genes within apple germplasm collections and can be used to increase the number of sources for disease resistance in breeding programmes. Keywords: apple, Czech genetic resources, Malus × domestica, Podosphaera leucotricha, powdery mildew, resistance PCR molecular markers, scab, Venturia inaequalis Journal: Czech Journal of Genetics and Plant Breeding Pages: 156-165 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/140/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/140/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201104-0005.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:140-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ivana POLIŠENSKÁ Author-Workplace-Name: Agrotest Fyto, Ltd., Kroměříž, Czech Republic Author-Name: Lenka NEDOMOVÁ Author-Workplace-Name: Agricultural Research Institute Kromeriz, Ltd., Kroměříž, Czech Republic Author-Name: Tomáš VYHNÁNEK Author-Workplace-Name: Mendel University in Brno, Brno, Czech Republic Title: Characterisation of oat genetic resources using electrophoresis of avenins Abstract: The prolamin (avenin) patterns of oat (Avena sativa L.) cultivars released in the Czech Republic and in the former Czechoslovak Republic were analysed by acid polyacrylamide gel electrophoresis (A-PAGE). Forty-nine oat (Avena sativa L.) accessions of domestic origin, maintained in the Czech collection of oat genetic resources, were analysed. The evaluated set contained 18 modern and 31 old cultivars. Thirty accessions showed a homogeneous prolamin pattern. The other accessions were heterogeneous with two or three different patterns, present at different percentages. Heterogeneity was present in 48% of the old cultivars, but only in 22% of the modern cultivars. Identity indexes within the heterogeneous accessions were calculated. The index values ranged from 0.09 to 0.75. Only in two heterogeneous cultivars (Dětenický Bílý, Valečovský Bílý) the identity index between their components was higher than 0.6, indicating, that their components were most likely sister lines. All analysed cultivars could be unambiguously distinguished by their prolamin pattern. The obtained prolamin patterns will be used to complete descriptor data of the genetic resources and might be useful also in oat breeding and research. Keywords: avenins, genotype distinguishing, line, oats, PAGE Journal: Czech Journal of Genetics and Plant Breeding Pages: 166-171 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/42/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/42/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201104-0006.txt Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:42-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: I.T. Prášil Author-Name: J. Chrpová Author-Name: Z. Stehno Title: Sixty years of the Crop Research Institute and its impact on plant breeding Journal: Czech Journal of Genetics and Plant Breeding Pages: 172-180 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/174/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/174/2011-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:174-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Z. Stehno Author-Name: L. Dotlačil Author-Name: I. Faberová Title: Ing. Ivo Bareš, DrSc. Journal: Czech Journal of Genetics and Plant Breeding Pages: 181 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/175/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/175/2011-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:175-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Horčička Author-Name: O. Veškrna Author-Name: T. Sedláček Author-Name: A. Hanzalová Author-Name: V. Šíp Title: Spring wheat Seance Journal: Czech Journal of Genetics and Plant Breeding Pages: 182-184 Volume: 47 Issue: 4 Year: 2011 DOI: 10.17221/163/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/163/2011-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:47:y:2011:i:4:id:163-2011-CJGPB