Template-Type: ReDIF-Article 1.0 Author-Name: Alena Hanzalová Author-Workplace-Name: Department of Genetics and Plant Breeding Methods, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Taťána Sumíková Author-Workplace-Name: Department of Genetics and Plant Breeding Methods, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jozef Huszár Author-Workplace-Name: Department of Biology, J. Selye University, Komárno, Slovak Republic Author-Name: Pavel Bartoš Author-Workplace-Name: Department of Genetics and Plant Breeding Methods, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Slovak Republic in 2009-2011 Abstract: In 2009-2011 virulence of the wheat leaf rust population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Slovak Republic. A total of 122 wheat leaf rust isolates were analysed. Resistance gene Lr19 was effective to all tested isolates. Virulence to Lr9 was found, however only in one isolate. Gene Lr24 conditioned resistance to almost all rust collections. A lower frequency of virulence to Lr2a and Lr28 was also observed. Nineteen winter wheat cultivars grown in Slovakia were tested with 8 leaf rust isolates. Winter wheat cultivar Bona Dea was resistant to all isolates applied in the greenhouse test. Presence of Lr genes was estimated according to the reactions of the tested cultivars. Presence of Lr10, Lr26, Lr34 and Lr37 was studied by molecular markers. Keywords: leaf rust pathotypes, Lr genes, resistance, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 101-107 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/28/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/28/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201203-0001.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:28-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Veronika DUMALASOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Leona SVOBODOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Alena HANZALOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Differentially expressed gene transcripts in wheat and barley leaves upon leaf spot infection Abstract: A cDNA library was created on the basis of transcripts that were generated during the process of infection of wheat and barley with Pyrenophora tritici-repentis or P. teres. Due to the time course of infection assays, the leaves were collected at various intervals after inoculation until 100 h after inoculation. We compared the temporal development of the two pathogens, as well as the effect of varieties of the same crop species, and differences among isolates of a single pathogen. The appressoria and vesicular structures developed more rapidly in barley varieties inoculated by P. teres than in wheat varieties inoculated by P. tritici-repentis. The lower level of susceptibility of the wheat variety Globus and the barley variety Heris to P. tritici-repentis and P. teres, respectively, was apparent from 12 h after infection. Information on changes in expression profiles during pathogenesis and on the clones that could be functionally assigned to the known gene sequences is presented. Out of the 360 transcript derived fragments (TDFs) produced in the barley-P. teres pathosystem in our trials, 62% were specific to the plant-pathogen interaction. In the wheat-P. tritici-repentis pathosystem 291 TDFs occurred, 63% were specific to the plant-pathogen interaction. Keywords: cDNA-AFLP, infection development, net blotch, Pyrenophora tritici-repentis, Pyrenophora teres, tan spot Journal: Czech Journal of Genetics and Plant Breeding Pages: 108-119 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/187/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/187/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201203-0002.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:187-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: A. Mohana PRIYA Author-Workplace-Name: Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India Author-Name: S. Karutha PANDIAN Author-Workplace-Name: Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India Author-Name: Ramesh MANIKANDAN Author-Workplace-Name: Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India Title: The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium-mediated transformation of indica rice (Oryza sativa L.) Abstract: We report here the suitable explant with high efficiency of transformation and the positive effects of timentin over other antibiotics like carbenicillin and cefotaxime on the elimination of Agrobacterium tumefaciens during the genetic transformation of popular indica rice (Oryza sativa L.). The tissues assayed were embryogenic calli, embryos with endosperm contamination, intact seeds, leaf blades, leaf bases and coleoptiles. The frequency of transient β-glucuronidase (GUS) expression as revealed by histochemical assay was 90% for embryogenic calli, which was the highest among the explants used. On the basis of disc-diffusion assay, the maximum zone of inhibition (29 mm) at 250 mg/l was obtained for timentin. In tissue culture conditions the frequency of Agrobacterium recurrence after 20 days of infection was minimum (2.3%) at 200 mg/l of timentin. At 250 mg/l of timentin there was no Agrobacterium growth, besides, there were no negative effects on the callus growth unlike other antibiotics, hence it was selected as the optimum concentration for high frequency callus proliferation and regeneration.The effect of the parameters evaluated was determined by the callus proliferation during selection, reduction in browning, transient GUS expression and stable transformation efficiency (23.3%). The resultant plants were stable transformants as confirmed by a molecular analysis of the gus and hpt genes. The developed transformation protocol will be very helpful for the information on indica rice cultivars in general and on IR 64 in particular. Keywords: Agrobacterium-mediated transformation, Agrobacterium elimination, gus reporter gene, hygromycin phosphotransferase selectable marker gene (hpt), rice Journal: Czech Journal of Genetics and Plant Breeding Pages: 120-130 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/77/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/77/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201203-0003.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:77-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mohammad Reza NAGHAVI Author-Workplace-Name: Agronomy and Plant Breeding Department, Agricultural College, University of Tehran, Karaj, Iran Author-Name: Sajad Rashidi MONFARED Author-Workplace-Name: Agronomy and Plant Breeding Department, Agricultural College, University of Tehran, Karaj, Iran Author-Name: Gomez HUMBERTO Author-Workplace-Name: Generation Challenge Program, CIMMYT, Mexico City, Mexico Title: Genetic diversity in Iranian chickpea (Cicer arietinum L.) landraces as revealed by microsatellite markers Abstract: To estimate the genetic diversity of chickpea germplasm from Iran, a total of 307 landraces from 4 regions including: northern areas (29 from Ardebil, 3 from Qazvin and 5 from Mazanderan provinces), temperate (16 from Kermanshah, 2 from Semnan, 54 from Khorasan and 20 from Kerman provinces), semi-arid (28 from Ghom and 56 from Isfahan provinces) and cold areas (15 from West Azarbayjan, 52 from Tehran and 27 from East Azarbayjan provinces) were analysed using 16 microsatellite loci. The number of alleles per microsatellite locus ranged from 8 to 29, with an average of 19.31 per locus. A high level of genetic diversity in the northern area (He = 0.76), even with a limited number of available landraces (37) compared with the other three regions (84-94), might confirm the northern Persia as part of the chickpea centre of origin. The neighbour-joining tree showed a low relationship between molecular divergence and the geographical grouping of chickpea. Moreover, cluster analyses based on molecular data showed that the northern area was separated clearly from the other three regions, indicating a physical barrier or geographical and environmental differences among these regions. A wide genetic diversity of Iranian chickpea landraces is a critical component for future selection and use of this germplasm for future breeding of chickpea. Keywords: Cicer arietinum, chickpea, genetic structure, germplasm, microsatellite Journal: Czech Journal of Genetics and Plant Breeding Pages: 131-138 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/185/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/185/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201203-0004.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:185-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lenka HAVLÍČKOVÁ Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Vladislav ČURN Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Eva JOZOVÁ Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Vratislav KUČERA Author-Workplace-Name: Crop Research Institute, Praha-Ruzyně, Czech Republic Author-Name: Miroslava VYVADILOVÁ Author-Workplace-Name: Crop Research Institute, Praha-Ruzyně, Czech Republic Author-Name: Miroslav KLÍMA Author-Workplace-Name: Crop Research Institute, Praha-Ruzyně, Czech Republic Title: Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.) Abstract: Until now in Europe has not been cultivated any hybrid cultivar of oilseed rape based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system in China. The aim of Czech breeders now is to produce new, improved cultivars of rapeseed based on this CMS system. Sterile Shaan 2A CMS line (S; rf/rf), its corresponding maintainers (N; rf/rf) and fertility restorers (S; Rf/Rf) were analyzed on molecular level for the presence of functional CMS cytoplasm. Two new primer pairs covering CMS-associated gene (so called orf224-1) present in Shaan 2A CMS line were developed and selection capability of the developed primers was successfully evaluated. These primers can be used for early selection of plants with functional Shaan 2A CMS system in breeding programmes. Keywords: cytoplasmic male sterility (CMS), marker-assisted selection (MAS), orf224-1, rapeseed (Brassica napus L.), Rf-restorer gene, Shaan 2A CMS line Journal: Czech Journal of Genetics and Plant Breeding Pages: 139-142 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/144/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/144/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201203-0005.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:144-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: A. Hanišová Author-Name: J. Bradová Author-Name: E. Schwarzbach Title: Prof. Ing. Jiří Černý, Ph.D. Journal: Czech Journal of Genetics and Plant Breeding Pages: 143 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/172/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/172/2012-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:172-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: P. Horčička Author-Name: O. Veškrna Author-Name: T. Sedláček Author-Name: A. Hanzalová Title: Spring wheat Dafne Journal: Czech Journal of Genetics and Plant Breeding Pages: 144-145 Volume: 48 Issue: 3 Year: 2012 DOI: 10.17221/175/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/175/2012-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:3:id:175-2012-CJGPB