Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 48 Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/6404-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6404-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:6404-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Kunjupillai VIJAYAN Author-Workplace-Name: Central Silk Board, BTM Layout, Madiwala, Bangalore, India Author-Name: Prem Prakash SRIVASTAVA Author-Workplace-Name: Central Silk Board, BTM Layout, Madiwala, Bangalore, India Author-Name: P. Jayarama RAJU Author-Workplace-Name: Central Silk Board, BTM Layout, Madiwala, Bangalore, India Author-Name: Beera SARATCHANDRA Author-Workplace-Name: Central Silk Board, BTM Layout, Madiwala, Bangalore, India Title: Breeding for higher productivity in mulberry Abstract: Mulberry (Morus L.) is an economically important tree being cultivated for its leaves to rear the silkworm Bombyx mori. Rearing of silkworm is an art and science popularly known as sericulture; an agrobased cottage industry provides employment to millions in China, India, Korea, Vietnam, etc. Mulberry is a perennial tree that maintains high heterozygosity due to the outbreeding reproductive system. It is recalcitrant to most of the conventional breeding methods, yet considerable improvement has been made in leaf yield and leaf quality. Conventional breeding in mulberry is a tedious, labour intensive and time taking process, which needs to be complemented with modern biotechnological methods to speed up the process. This article enumerates the problems, challenges, constraints and achievements in mulberry breeding along with recent advances in biotechnology and molecular biology to enable mulberry breeders to tackle specific problems more systematically and effectively. Keywords: association mapping, QTL mapping, Morus L., sericulture Journal: Czech Journal of Genetics and Plant Breeding Pages: 147-156 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/162/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/162/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201204-0002.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:162-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Daniel Mihálik Author-Workplace-Name: Plant Production Research Center Piešťany, Piešťany, Slovak Republic Author-Name: Edita Gregová Author-Workplace-Name: Plant Production Research Center Piešťany, Piešťany, Slovak Republic Author-Name: Petr Galuszka Author-Workplace-Name: Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Ludmila Ohnoutková Author-Workplace-Name: Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Tatiana Klempová Author-Workplace-Name: Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak Technical University, Bratislava, Slovak Republic Author-Name: Katarína Ondreičková Author-Workplace-Name: Plant Production Research Center Piešťany, Piešťany, Slovak Republic Author-Name: Marcela Gubišová Author-Workplace-Name: Plant Production Research Center Piešťany, Piešťany, Slovak Republic Author-Workplace-Name: Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: Jozef Gubiš Author-Workplace-Name: Plant Production Research Center Piešťany, Piešťany, Slovak Republic Author-Name: Ján Kraic Author-Workplace-Name: Plant Production Research Center Piešťany, Piešťany, Slovak Republic Title: Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.) Abstract: A novel high-molecular-weight glutenin subunit encoded by the Glu-1D locus was identified in hexaploid wheat (Triticum aestivum L.) cultivar Noe and was designated as 1Dy12.3. This subunit differed in SDS-PAGE mobility from the well-known 1Dy10 and 1Dy12 subunits that are also encoded by this locus. An analysis of the gene sequences confirmed the uniqueness of 1Dy12.3 and revealed that it is most closely related to the 1Dy12 subunit. The size of the deduced protein was calculated to be 67 884 Da, which is different from the 1Dy10 and 1Dy12 subunits (67 475 Da and 68 713 Da, respectively). The 1Dy12.3 protein consists of 652 residues, with a highly conserved signal sequence and N- and C-terminal domains, although the central repetitive domain comprising motifs of hexapeptide (PGQGQQ) and nonapeptide (GYYPTSLQQ) repeats was less conserved. The 1Dy12.3 subunit demonstrates fewer QHPEQG hexapeptide motifs and exhibits an increased number of methionine residues in comparison to the other characterised high-molecular-weight glutenin subunits. The 1Dy12.3 subunit was cloned and expressed in Escherichia coli and was detected with a prolamin-specific antibody. The size of the detected immunocomplex corresponded to the native 1Dy12.3 protein isolated from grains. The existence and characterisation of this novel high-molecular-weight glutenin subunit increases the diversity of the glutenins encoded by the Glu-1D locus. Keywords: 1Dy12.3, Glu-1Dy locus, high-molecular-weight glutenin subunit, Triticum aestivum L Journal: Czech Journal of Genetics and Plant Breeding Pages: 157-168 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/111/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/111/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201204-0003.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:111-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Annamária SCHNEIDER Author-Workplace-Name: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Author-Name: Márta MOLNÁR-LÁNG Author-Workplace-Name: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Title: Detection of various U and M chromosomes in wheat-Aegilops biuncialis hybrids and derivatives using fluorescence in situ hybridisation and molecular markers Abstract: The aim of the study was to select wheat-Aegilops biuncialis addition lines carrying Aegilops biuncialis chromosomes differing from those which were introgressed into the wheat-Ae. biuncialis addition lines produced earlier in Martonvásár, Hungary. In the course of the experiments new wheat-Ae. biuncialis addition lines carrying chromosomes 2Ub, 6Mb, 6Ub; 5Ub, 3Ub, 7Ub; 5Mb, 6Mb and 7Mb were selected. The 2Ub disomic addition line is relatively stable, as 91% of the progenies contain this chromosome pair. The 6Mb disomic addition line proved to be dwarf and sterile, but it still exists as a monosomic addition line. Progenies analysed from the 6Ub monosomic addition line did not carry the 6Ub chromosome. One plant containing the 5Ub, 3Ub and 7Ub chromosomes and one plant carrying 5Mb, 6Mb and 7Mb chromosomes showed very low fertility. Each of the plants produced a single seed, but seeds of the parent plants are still available. Line No. 49/00 carried a submetacentric Ae. biuncialis chromosome pair and the chromosome number 44 has been constant for several generations. After FISH no hybridisation site was observed on the Ae. biuncialis chromosome pair using the pSc119.2 and Afa family repetitive DNA probes, so it was not possible to identify the Ae. biuncialis chromosome pair. However, the use of wheat SSR markers and the (GAA)n microsatellite DNA probe allowed it to be characterised more accurately. These new lines facilitate gene transfer from Ae. biuncialis into cultivated wheat and the selection of U and M genome-specific wheat SSR markers. Keywords: addition lines, FISH polymorphism, goatgrass, wheat SSR markers Journal: Czech Journal of Genetics and Plant Breeding Pages: 169-177 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/45/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/45/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201204-0004.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:45-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Muhammad Abdul Rab Faisal SULTAN Author-Workplace-Name: State Key Laboratory of Crop Stress Biology for Arid Areas, Author-Workplace-Name: College of Life Sciences, Northwest A & F University, Shaanxi, Yangling, China Author-Name: Liu HUI Author-Workplace-Name: State Key Laboratory of Crop Stress Biology for Arid Areas, Author-Workplace-Name: College of Life Sciences, Northwest A & F University, Shaanxi, Yangling, China Author-Name: Lv Jin YANG Author-Workplace-Name: State Key Laboratory of Crop Stress Biology for Arid Areas, Author-Workplace-Name: College of Life Sciences, Northwest A & F University, Shaanxi, Yangling, China Author-Name: Zhao Hui XIAN Author-Workplace-Name: State Key Laboratory of Crop Stress Biology for Arid Areas, Author-Workplace-Name: College of Life Sciences, Northwest A & F University, Shaanxi, Yangling, China Title: Assessment of drought tolerance of some Triticum L. species through physiological indices Abstract: Wheat is one of the most important crops in the world. Its yield is greatly influenced by global climate change and scarcity of water in the arid and semi-arid areas of the world. So, exploration of gene resources is of importance to wheat breeding in order to improve the crop ability of coping with abiotic stress environment. Wild relatives of wheat are rich repositories of beneficial genes that confer tolerance or resistance not only to drought but also to other environmental stresses. In the present study, the changes in leaf relative water content (RWC), free proline content, and malondialdehyde (MDA) accumulation of five wild wheat species including T. boeticum (YS-1L), T. dicoccum var. dicoccoides (YS-2L), T. araraticum (ALLT), and two cultivated varieties of T. turgidum ssp. durum (MXLK and 87341), with two well-known common wheat cultivars (SH6 and ZY1) possessing strong drought resistance and sensitiveness, respectively, as references were investigated during 3-day water stress and 2-day recovery, in order to assess the drought tolerance of these wild wheat species. The laboratory experiment was conducted under two water regimes (stress and non-stress treatments). Stress was induced to hydroponically grown two weeks old wheat seedlings with 20% PEG 6000. Stress treatment caused a much smaller decrease in the leaf RWC and rise in MDA content in YS-1L compared to the other wheat species. From the data it was obvious that YS-1L was the most drought tolerant among studied species having significantly higher proline and RWC while lower MDA content under water stress conditions. The order of water stress tolerance of these species according to the three parameters is: YS-1L > YS-2L > SH6 > 87341 > ZY1 > MXLK > ALLT. We speculate that the observed drought stress tolerance at a cellular level was associated with the ability to accumulate proline and high water level conservation. Keywords: drought tolerance, MDA, proline, relative water content, Triticum L. species, water stress Journal: Czech Journal of Genetics and Plant Breeding Pages: 178-184 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/21/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/21/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201204-0005.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:21-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Antonín DREISEITL Author-Workplace-Name: Agrotest Fyto Ltd., Kroměříž, Czech Republic Title: Identity of barley powdery mildew resistances Bw and Ru2 Abstract: A large number of resistances to powdery mildew (Blumeria graminis f.sp. hordei) and their combinations are known in barley (Hordeum vulgare L.). A similarity of resistance spectra between cultivars carrying the resistance Bw, designated for the winter barley cultivar Borwina, and the near-isogenic spring barley line P15, which carries the resistance Ru2, derived from the landrace Rupee, was found. The objective of this study was to test the difference between resistances Bw and Ru2. Six cultivars were tested, four with Bw and two with Ru2. Testing with 40 isolates showed identical reaction spectra between both groups. Testing of the cultivar Kompolti 4 (Bw) and line P15 (Ru2) with 300 isolates confirmed this result. Thus, the resistances currently designated Bw and Ru2 can be regarded as identical, and are determined by the gene Ml(Ru2). Both Bw and Ru2 should be designated by the earlier code, Ru2. Keywords: Blumeria graminis f.sp. hordei, Hordeum vulgare, pathogen isolates, resistance gene postulation Journal: Czech Journal of Genetics and Plant Breeding Pages: 185-188 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/60/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/60/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201204-0006.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:60-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jana CHRPOVÁ Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Václav ŠÍP Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Pavel BARTOŠ Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Alena HANZALOVÁ Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jana PALICOVÁ Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Lenka ŠTOČKOVÁ Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Lubomír ČEJKA Author-Workplace-Name: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Irena BÍŽOVÁ Author-Workplace-Name: SELTON, Ltd., Úhřetice Breeding Station, Czech Republic Author-Name: Petr LAML Author-Workplace-Name: RAGT Czech, Ltd., Branišovice Breeding Station, Czech Republic Author-Name: Tomáš NOVÁČEK Author-Workplace-Name: Limagrain Central Europe Cereals, Ltd., Hrubčice, Czech Republic Author-Name: Pavel HORČIČKA Author-Workplace-Name: SELGEN a.s., Stupice Breeding Station, Czech Republic Title: Results of the Czech National Ring Tests of disease resistance in wheat Abstract: In this contribution actual results of the Czech national ring tests of disease resistance in wheat are presented that are performed at 3-5 locations each year. Special attention was paid to possibilities of increasing resistance to rusts, powdery mildew, Fusarium head blight and brown leaf spot diseases. New sources of resistance to the above-mentioned diseases were detected and described. Achievements and prospects of wheat breeding for resistance to these diseases, as well as to other important diseases and pests (common and dwarf bunt, eyespot and stem base diseases, barley yellow dwarf virus, orange wheat blossom midge) are discussed. Keywords: Czech Republic, diseases and pests, resistance genes, resistance sources, Triticum aestivum L Journal: Czech Journal of Genetics and Plant Breeding Pages: 189-199 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/173/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/173/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201204-0007.txt Handle: RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:173-2012-CJGPB