Template-Type: ReDIF-Article 1.0 Author-Name: Xiaojie YANG Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Workplace-Name: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China Author-Name: LI Fuguang Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Xueyan ZHANG Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Kun LIU Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Qianhua WANG Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Chaojun ZHANG Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Chuanliang LIU Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Wei ZHU Author-Workplace-Name: Agronomy College of Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Guofang SHAN Author-Workplace-Name: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China Author-Name: Chee-Kok CHIN Author-Workplace-Name: Department of Plant Science, Rutgers University, New Jersey, USA Author-Name: Weiping FANG Author-Workplace-Name: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China Title: Integration and characterization of T-DNA insertion in upland cotton Abstract: Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed co-transformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites. Keywords: cotton (Gossypium hirsutum L.), deletion of border, genetic transformation, transgene copy, vector integration Journal: Czech Journal of Genetics and Plant Breeding Pages: 51-57 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/217/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/217/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0001.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:217-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Shui-Ping LIU Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Yong-Bo HONG Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: WU Zhen Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Yong-Shuo MA Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Deng-Wei JUE Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Chao XIE Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Yan-Ping ZHU Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Min CHEN Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Author-Name: Qing YANG Author-Workplace-Name: College of Life Sciences, Nanjing Agricultural University, Nanjing, P.R. China Title: Protein extract of tobacco expressing StoVe1 gene inhibits Verticillium dahliae proliferation Abstract: Verticillium dahliae is a principal pathogen causing verticillium wilt in Solanaceae crops. StoVe1 is a gene resisting to verticillium wilt isolated from Solanum torvum. In order to generate resistant tobacco plants, StoVe1 was inserted in the orientation behind CaMV 35S promoter of vector pGS and this construct was introduced into tobacco by Agrobacterium-mediated transformation. A total of 12 kanamycin-resistant plants were generated and 7 independent transgenic lines were identified by PCR analysis. Quantitative RT-PCR analysis showed that the levels of StoVe1 transcript in transgenic lines were up to 2-6 fold higher than in the control. Anti-fungal assay indicated that the protein extract of transgenic tobacco lines showed strong inhibition activity to V. dahliae, 2 fold or higher compared to control plants. This result reveals that StoVe1, as a V. dahliae resistance gene, has an application potential in plant breeding for verticillium wilt resistance. Keywords: disease resistance, regeneration, StoVe1, transformation, tobacco Journal: Czech Journal of Genetics and Plant Breeding Pages: 58-64 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/165/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/165/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0002.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:165-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslav SALAVA Author-Workplace-Name: Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jaroslav POLÁK Author-Workplace-Name: Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Ivan OUKROPEC Author-Workplace-Name: Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Title: Evaluation of the Prunus interspecific progenies for resistance to Plum pox virus Abstract: Sharka disease caused by the infection with the Plum pox virus (PPV) in stone fruit trees is worldwide the most devastating for stone fruit production. Until now, good sources of resistance to PPV within the peach group have not been available. There are no commercial cultivars of peach that are resistant to PPV. Other Prunus species are known to show varying levels of resistance. Interspecific hybrids GF 677 (Prunus amygdalus × P. persica) and Cadaman (P. davidiana × P. persica) were revealed to be resistant to PPV. The resistance to a Dideron isolate of the descendants of Cresthaven × GF 677 and Cresthaven × Cadaman and their progenitors was evaluated after inoculation by chip-budding in a sealed screenhouse. Results demonstrate a certain level of resistance in both progenies of interspecific hybrids and indicate a potential for PPV resistance transfer to commercial peach cultivars but it will be necessary to perform backcrosses with peach cultivars of agricultural interest in order to return pomological and agronomic traits. For the definitive confirmation of resistance/susceptibility it will be necessary to wait until the adult stage of hybrids. Keywords: Prunus amygdalus, Prunus davidiana, Prunus persica, PPV, sharka disease, transmissibility Journal: Czech Journal of Genetics and Plant Breeding Pages: 65-69 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/252/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/252/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0003.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:252-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Miluše SVOBODOVÁ Author-Workplace-Name: Department of Forage Crops and Grassland Management, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jaroslav MARTINEK Author-Workplace-Name: Department of Forage Crops and Grassland Management, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Tereza KRÁLÍČKOVÁ Author-Workplace-Name: Department of Forage Crops and Grassland Management, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Ivo NAŠINEC Author-Workplace-Name: Plant Breeding Station Větrov, Oseva Uni, Choceň, Czech Republic Author-Name: Jaromír ŠANTRŮČEK Author-Workplace-Name: Department of Forage Crops and Grassland Management, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Competition ability of selected amenity varieties of Festuca rubra in mixture with Deschampsia caespitosa Abstract: The aim of the research was to evaluate the competition capacity and find suitable proportion of different types of Festuca rubra and Deschampsia caespitosa in grass mixtures for decorative lawns. A field experiment with Deschampsia caespitosa cv. Kometa in monoculture or in mixtures with Festuca rubra L. varieties Viktorka (ssp. trichophylla), Barborka (ssp. commutata) and Petruna (ssp. rubra) was established in Větrov in 2007. The sowing rate was 40 000 viable seeds per m2 (0, 25, 50 or 75% of F. rubra). The sward was mown at 3 cm. The number and weight of tillers of the turf components were evaluated during three years. The highest number of tillers (on average 63.3 thousand/m2) and weight of the dry aboveground phytomass (on average 196 g/m2) were found with F. rubra ssp. trichophylla. Its particular tillers had the lowest dry weight (0.32 g/100 tillers). The lowest number of tillers (32.9 thousand/m2) and low weight of dry aboveground phytomass (134 g/m2) showed mixtures with F. rubra ssp. rubra, whose tiller weight was relatively high (0.42 g/100 tillers). The size of F. rubra tillers was not influenced either by the mixture composition or by vegetation years. The number of F. rubra tillers was not significantly different in the third and first vegetation year. The number of D. caespitosa tillers in monoculture decreased during the years from 45 to 30 thousand/m2, but the weight of dry aboveground phytomass increased significantly from 233 g/m2 to 318 g/m2. Poor competition of D. caespitosa was found in the mixture with F. rubra ssp. commutata (on average 4% of total number of tillers and 7% of the total weight of the dry aboveground phytomass). F. rubra ssp. trichophylla was less competitive and the least competition with D. caespitosa was found in F. rubra ssp. rubra, After three years the proportion of D. caespitosa in mixtures with F. rubra ssp. rubra significantly increased to 36% of the total number of tillers and to 55% of the total weight of phytomass. Mixtures of D. caespitosa and F. rubra ssp. rubra were found to be promising for low input lawns. Keywords: competition, cultivars, lawn density, red fescue, tufted hairgrass, turfgrass Journal: Czech Journal of Genetics and Plant Breeding Pages: 70-76 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/86/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/86/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0004.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:86-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mónika CSÉPLŐ Author-Workplace-Name: Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Author-Name: Mária CSŐSZ Author-Workplace-Name: Cereal Research Non-Profit, Ltd., Szeged, Hungary Author-Name: Mariann GÁL Author-Workplace-Name: Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Author-Name: Ottó VEISZ Author-Workplace-Name: Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Author-Name: Gyula VIDA Author-Workplace-Name: Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Title: Seedling resistance to Stagonospora nodorum blotch in wheat genotypes Abstract: In two independent experiments set up in the greenhouse the seedling resistance to Stagonospora nodorum blotch was investigated in 92 varieties, breeding lines and genotypes with a known genetic background. The greatest area under the disease progress curve calculated from lesion type was 37.06, while in the case of the most resistant genotype this value was 0.38. Many of the lines and varieties bred in Martonvásár proved to have excellent resistance in terms of both percentage of infected leaf area and lesion type. Observations indicate that, depending on the aim of the experiment, the efficient selection of breeding lines is possible in the seedling stage either on the basis of the area under the disease progress curve calculated for lesion types, or on the basis of lesion types scored 7, 11 or 14 days after inoculation. Keywords: area under the disease progress curve, disease resistance, Phaeosphaeria nodorum, Triticum aestivum Journal: Czech Journal of Genetics and Plant Breeding Pages: 77-85 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/69/2011-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/69/2011-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0005.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:69-2011-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Girjesh KUMAR Author-Workplace-Name: Plants Genetics Laboratory, Department of Botany, University of Allahabad, Allahabad, India Author-Name: Sana NASEEM Author-Workplace-Name: Plants Genetics Laboratory, Department of Botany, University of Allahabad, Allahabad, India Title: EMS induced intercellular chromatin transmigration in Papaver somniferum L. Abstract: The phenomenon of chromatin migration was observed during microsporogenesis in an ethyl methane sulphonate (EMS) treated population of poppy, which is an important medicinal plant. Cytomixis occurred through a cytoplasmic channel or by direct fusion of pollen mother cells (PMCs); the former was more recurring than the latter. The process was associated with irregular meiosis. PMCs with differing chromosome numbers from the normal diploid number (2n = 22) through cytomixis may lead to the production of aneuploid and polyploid gametes. An increase in the concentration of EMS had a positive effect on the percentage of PMCs showing cytomixis. In addition to cytomixis, other chromosomal abnormalities were also found. Cytomixis along with the related chromosomal abnormalities largely affected the post-meiotic products resulting in some pollen sterility. Keywords: cytomixis, ethyl methane sulphonate, microsporogenesis, pollen sterility, poppy Journal: Czech Journal of Genetics and Plant Breeding Pages: 86-89 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/85/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/85/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0006.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:85-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Petr MARTINEK Author-Workplace-Name: Agrotest Fyto, Ltd. Kroměříž, Czech Republic Author-Name: Miroslav ŠKORPÍK Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jana CHRPOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Pavel FUČÍK Author-Workplace-Name: Branišovice, Czech Republic Author-Name: Josef SCHWEIGER Author-Workplace-Name: Saatbau Linz, OÖ. Landes-Saatbaugenossenschaft, GmbH., Austria Title: Development of the new winter wheat variety Skorpion with blue grain Abstract: Breeding wheat with blue grain was conducted at the Crop Research Institute in Prague. Initial donor material came from the legacy of Erich von Tschermak-Seysenegg. Long-term crosses with a series of winter wheat varieties were made with the aim of transferring blue grain colour into cultivated varieties. The prospective material was later handed over to Agrotest Fyto, Ltd., Kroměříž, where line no. 6 was selected from the population RU 440. At the end of 2011, the new winter wheat variety Skorpion with blue grain was registered in Austria. It is intended for special use in the food industry. The anthocyanins which it contains are considered to offer health benefits due to their antioxidant effects. Keywords: agronomic traits, anthocyanins, blue aleurone, grain quality, new variety, Triticum aestivum L Journal: Czech Journal of Genetics and Plant Breeding Pages: 90-94 Volume: 49 Issue: 2 Year: 2013 DOI: 10.17221/7/2013-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/7/2013-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201302-0007.txt Handle: RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:7-2013-CJGPB