Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: LIST OF REVIEWERS - 2013 Journal: Czech Journal of Genetics and Plant Breeding Pages: I Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/7239-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/7239-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:7239-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Kafeel AHMAD Author-Workplace-Name: Department of Biology, University of Leicester, Leicester, United Kingdom Title: Molecular farming: strategies, expression systems and bio-safety considerations Abstract: Molecular farming is an experimental application of biotechnology that involves the genetic modification of crops for the production of proteins and chemicals for medicinal and commercial purposes. The vast majority in the developing world cannot afford the high cost of therapeutics produced by existing methods. We need to produce not only new therapeutics but also cheaper versions of the existing ones. Molecular farming could offer a viable option for this growing need for biopharmaceuticals. Plant made therapeutics are cheaper, safer, can be abundantly produced and easily stored. Here, strategies and approaches utilized in plant molecular farming are discussed. Furthermore, the bio-safety considerations related to this emerging field are also discussed. Keywords: biopharmaceuticals, recombinant proteins, rhizosecretion, therapeutics, transformation Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-10 Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/187/2013-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/187/2013-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201401-0002.txt Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:187-2013-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Semra HASANCEBI Author-Workplace-Name: Genetic Engineering and Biotechnology Institute (GEBI), Marmara Research Center (MRC), Scientific and Technological Research Council of Turkey (TUBITAK), Kocaeli, Turkey Author-Name: Zafer MERT Author-Workplace-Name: Field Crop Research Institute, Ankara, Turkey Author-Name: Fahriye ERTUGRUL Author-Workplace-Name: Agriculture, Forestry and Veterinary Research Group, Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, Turkey Author-Name: Kadir AKAN Author-Workplace-Name: Field Crop Research Institute, Ankara, Turkey Author-Name: Yildiz AYDIN Author-Workplace-Name: Department of Biology, Faculty of Arts and Science, Marmara University, Istanbul, Turkey Author-Name: Funda SENTURK AKFIRAT Author-Workplace-Name: Department of Molecular Biology and Genetics, Faculty of Science, Gebze Institute of Technology, Kocaeli, Turkey Author-Name: Ahu ALTINKUT UNCUOGLU Author-Workplace-Name: Department of Bioengineering, Faculty of Engineering, Marmara University, Istanbul, Turkey Title: An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat Abstract: EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) × ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 × Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene. Keywords: Bulk Segregant Analysis (BSA), Marker-Assisted Selection (MAS), Puccinia striiformis f.sp. tritici, Triticum aestivum L., Yr1 Journal: Czech Journal of Genetics and Plant Breeding Pages: 11-18 Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/109/2013-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/109/2013-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201401-0003.txt Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:109-2013-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lushui Weng Author-Workplace-Name: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, P.R. China Author-Name: Lihua Deng Author-Workplace-Name: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, P.R. China Author-Name: Fengxiang Lai Author-Workplace-Name: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, P.R. China Author-Name: Guoying Xiao Author-Workplace-Name: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, P.R. China Title: Optimization of the Cry2Aa gene and development of insect-resistant and herbicide-tolerant photoperiod-sensitive genic male sterile rice Abstract: In this study, an optimized Cry2Aa gene was obtained after codon optimization based on the preferred codons in rice. The novel fusion gene Cry2Aa# was designed by adding the sequence coding the signal peptide of PR1a at the 5' end and the endoplasmic reticulum retention signal peptide KDEL at the 3' end of the optimized Cry2Aa gene, respectively. The Cry2Aa# and Bar genes were transformed into 4008S, a photoperiod-sensitive genic male sterile (PGMS) line in rice, using the Agrobacterium transfer method. A total of 65 regenerated plantlets confirmed by PCR were produced, in which eight transgenic lines had single-copy insertions as confirmed by Southern blot analysis. The high variability of Cry2Aa# gene expression was observed among independent transgenic lines with single-copy insertion, and the spatiotemporal difference of Cry2Aa protein expression was discovered in each transgenic line. The results showed that the transgenic lines were highly resistant to glufosinate, rice leaf roller and striped stem borer, which not only confirmed the effective optimization of the Cry2Aa gene but also produced a useful germplasm for breeding insect-resistant and herbicide-tolerant hybrid rice varieties. Keywords: Bar gene, Cry2Aa# gene, herbicide tolerance, insect resistance, PGMS line, rice Journal: Czech Journal of Genetics and Plant Breeding Pages: 19-25 Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/170/2012-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/170/2012-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201401-0004.txt Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:170-2012-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Nguyen Hoang LOC Author-Workplace-Name: Institute of Resources, Environment and Biotechnology, Hue University, Hue, Vietnam Author-Name: Dang Thanh LONG Author-Workplace-Name: Institute of Resources, Environment and Biotechnology, Hue University, Hue, Vietnam Author-Name: Tae-Geum KIM Author-Workplace-Name: Department of Molecular Biology, Chonbuk National University, Jeonju, Republic of Korea Author-Name: Moon-Sik YANG Author-Workplace-Name: Department of Molecular Biology, Chonbuk National University, Jeonju, Republic of Korea Author-Workplace-Name: Jeonju Center, Korea Basic Science Institute, Jeonju, Republic of Korea Title: Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L.) fruit Abstract: We report a feasibility study for expressing the LTB protein (Escherichia coli heat-labile enterotoxin B subunit) via Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.). We produced five regenerated plants obtained on the selection medium supplemented with an antibiotic. Stable integrations of the LTB gene into the genome of these plants were confirmed by Southern blot hybridization. Western blot analysis showed that only two of the five T0 transgenic tomato plants expressed the pentameric LTB protein in the fruits. An enzyme-linked immunosorbent assay indicated that these two plants synthesized the LTB protein bound specifically to GM1 ganglioside, suggesting that the LTB subunits formed active pentamers. The LTB protein produced in tomatoes can be a potential candidate for inexpensive, safe, and effective plant-based vaccines. Keywords: B subunit of E. coli heat-labile enterotoxin (LTB), LTB gene, Solanum lycopersicum, plant-based vaccine, tomato fruit, transgenesis Journal: Czech Journal of Genetics and Plant Breeding Pages: 26-31 Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/77/2013-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/77/2013-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201401-0005.txt Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:77-2013-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Qing-He CAO Author-Workplace-Name: Sweetpotato Research Institute, China Academy of Agricultural Science, Xuzhou, Jiangsu, P.R. China Author-Workplace-Name: Jiangsu Xuhuai Regional Xuzhou Institute of Agricultural Sciences, Xuzhou, Jiangsu, P.R. China Author-Name: Jun TANG Author-Workplace-Name: Sweetpotato Research Institute, China Academy of Agricultural Science, Xuzhou, Jiangsu, P.R. China Author-Workplace-Name: Jiangsu Xuhuai Regional Xuzhou Institute of Agricultural Sciences, Xuzhou, Jiangsu, P.R. China Author-Name: LI Ang Author-Workplace-Name: Sweetpotato Research Institute, China Academy of Agricultural Science, Xuzhou, Jiangsu, P.R. China Author-Workplace-Name: Jiangsu Xuhuai Regional Xuzhou Institute of Agricultural Sciences, Xuzhou, Jiangsu, P.R. China Author-Name: Wolfgang GRUNEBERG Author-Workplace-Name: International Potato Center (CIP), Lima, Peru Author-Name: Kelvin HUAMANI Author-Workplace-Name: International Potato Center (CIP), Lima, Peru Author-Name: MA Daifu Author-Workplace-Name: Sweetpotato Research Institute, China Academy of Agricultural Science, Xuzhou, Jiangsu, P.R. China Author-Workplace-Name: Jiangsu Xuhuai Regional Xuzhou Institute of Agricultural Sciences, Xuzhou, Jiangsu, P.R. China Title: Ploidy level and molecular phylogenic relationship among novel Ipomoea interspecific hybrids Abstract: Interspecific hybridization can be used to broaden the genetic base, generate novel species, postulate genetic relationships, and to introgress elite alien genes. However, interspecific hybridizations using wild parents outside the Ipomoea section Batatas are very difficult and have not been much studied. We used an improved hybridization technology to generate three novel interspecific hybrids by crossing Ipomoea batatas (L.) Lam. × I. hederacea Jacq., I. batatas (L.) Lam. × I. muricata (L.) Jacq., and I. batatas (L.) Lam. × I. lonchophylla J.M. Black. The ploidy level of the interspecific hybrids was determined by flow cytometry. The cross, I. batatas × I. hederacea, yielded the first artificial pentaploid Ipomoea hybrid ever. The other two hybrids, I. batatas × I. hederacea and I. batatas × I. muricata were tetraploid. The first two hybrids showed normal storage roots, a significant improvement in the storage roots of currently existing interspecific Ipomoea hybrids. AFLP (Amplified Fragment Length Polymorphism) molecular markers were used to explore the genetic relationship of these three novel interspecific hybrids with three other natural diploid, tetraploid, and hexaploid species of the Ipomoea section Batatas. Cluster analysis of AFLP bands showed that these three new interspecific hybrids were closely related to cultivated sweet potato (I. batatas/L./Lam.), which indicated that these novel hybrids can be used as an interspecific bridge to transfer alien genes from wild to cultivated species. Keywords: Amplified Fragment Length Polymorphism (AFLP), breeding, phylogeny, flow cytometry, sweet potato Journal: Czech Journal of Genetics and Plant Breeding Pages: 32-38 Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/172/2013-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/172/2013-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201401-0006.txt Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:172-2013-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: R. Šafaříková Title: List of field crop varieties registered in the Czech Republic in 2013 Journal: Czech Journal of Genetics and Plant Breeding Pages: 39-40 Volume: 50 Issue: 1 Year: 2014 DOI: 10.17221/22/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/22/2014-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:1:id:22-2014-CJGPB