Template-Type: ReDIF-Article 1.0 Author-Name: Antonín DREISEITL Author-Workplace-Name: Agrotest Fyto Ltd., Kroměříž, Czech Republic Title: Rare virulences of barley powdery mildew found in aerial populations in the Czech Republic from 2009 to 2014 Abstract: In breeding plants for resistance to disease pathogens major genes are often used. They mostly confer a resistance, which can be overcome by a corresponding pathogen virulence specific to the resistance conferred by that gene. Therefore, monitoring of new virulences in the pathogen population is an integral part of both the study of resistance and practical breeding. The aim of this paper is to present the detection of rare virulences that have been found in aerial populations of Blumeria graminis f.sp. hordei collected in the Czech Republic during the last six years. In total, 822 random isolates originating from cultivated spring and winter barley fields were evaluated on 37 differential varieties and 12 isolates carrying rare virulences were found. At least four virulences to resistances in Laverda, Psaknon, Venezia and SJ048311 were novel. In contrast, four rare isolates avirulent to the resistance Ha were also found. No isolate avirulent to resistances Ra, HH (a8) and Lo was detected. Monitoring of new virulences allows the selection of resistant donors for breeding new varieties and the identification of new resistances. Keywords: Blumeria graminis f.sp. hordei, Hordeum vulgare, specific resistances Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-8 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/254/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/254/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201501-0001.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:254-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Asma MEDOURI Author-Workplace-Name: Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine 1, Constantine, Algérie Author-Name: Inès BELLIL Author-Workplace-Name: Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine 1, Constantine, Algérie Author-Name: Douadi KHELIFI Author-Workplace-Name: Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine 1, Constantine, Algérie Title: The genetic diversity of gliadins in Aegilops geniculata from Algeria Abstract: The gliadins of the wild wheat Aegilops geniculata represent a valuable gene pool in breeding for bread making quality. The genetic diversity of gliadins in A. geniculata was studied among 36 of its accessions, collected in the north of Algeria, using acid polyacrylamide-gel electrophoresis (Acid-PAGE). In total, sixty-one polymorphic bands and 35 gliadin patterns were identified. Twenty-eight different bands and 34 patterns were found in the ω-gliadin region, 13 polymorphic bands and 33 patterns for γ-gliadins, 12 bands and 34 different patterns for β-gliadins and eight bands in combination resulted in 25 different patterns in the α-gliadin zone. Thirty-five patterns were found for each of the Gli-1 (γ/ω region) and Gli-2 (α/β region) loci. The genetic diversity index (H) was higher for ω-gliadins (0.968), followed by γ- and β-gliadins (0.964 and 0.961, respectively), and the lowest value was detected in α-gliadin patterns (0.944). Cluster analysis based on Ward's method divided the analysed collection into five separated groups in which genetic diversity did not follow the geographical distribution. The polymorphism observed in the electrophoretic patterns highlights close correlations between bioclimatic features and some ω-gliadin proteins. Keywords: Acid-PAGE, gliadin patterns, polymorphism, wild wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 9-15 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/158/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/158/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201501-0002.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:158-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Yu-Feng CHENG Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Life Sciences and Author-Workplace-Name: Baotou Light Industry Vocational Technical College, Baotou, Inner Mongolia, P.R. China Author-Name: Jian-Min CUI Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: LI Zhan-Jie Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Life Sciences and Author-Name: HU Zhao-Xin Author-Workplace-Name: College of Science and Engineering, City University of Hong Kong, Kowloon, Hong Kong, P.R. China Author-Name: Ze-Nong XING Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Jiao WANG Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Hui-Xian ZHAO Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Life Sciences and Author-Name: HU Sheng-Wu Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas, Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Title: The sulphonylurea herbicide monosulphuron ester sodium as a special male gametocide in Brassica napus L. Abstract: The sulphonylurea herbicide monosulphuron ester sodium (MES) was first used to induce male sterility in rapeseed (Brassica napus L.). Nearly 100% male sterility was achieved after a single treatment with 10 ml of 0.05-0.10 μg/ml MES solution per plant to the cultivars Zhongshuang No.9 and Zhongshuang No.11 at the uninucleate stage of the longest buds. No adverse effects on main agronomic traits and female fertility were observed. In the subsequent experiment, 48 out of 49 different accessions showed male sterility after spraying with 10 ml of 0.10 μg/ml MES solution per plant. Seven out of these 48 accessions were male sterile for the entire flowering period. Seed number per plant of these MES-treated plants was not significantly reduced compared to the controls. The hybridity was 95.5% in the case of seeds harvested from the ZS09 inbred line if sprayed twice with 10 ml of 0.10 µg/ml MES solution per plant and pollinated by the SH11 male line. These results suggest that MES is an efficient chemical hybridising agent for B. napus. Keywords: chemical hybridising agent, male sterility, MES, rapeseed Journal: Czech Journal of Genetics and Plant Breeding Pages: 16-22 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/233/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/233/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201501-0003.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:233-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jan BLAŽEK Author-Workplace-Name: Research and Breeding Institute of Pomology in Holovousy, Ltd., Holovousy, Czech Republic Author-Name: František PAPRŠTEIN Author-Workplace-Name: Research and Breeding Institute of Pomology in Holovousy, Ltd., Holovousy, Czech Republic Author-Name: Jana KŘELINOVÁ Author-Workplace-Name: Research and Breeding Institute of Pomology in Holovousy, Ltd., Holovousy, Czech Republic Title: Heritability of flowering time within apple progeny Abstract: The flowering onset of 19 apple progeny was evaluated in 2005-2014 on a total number of 715 seedlings in comparison with their parents and grandparents. A very low flower set delays generally the onset of flowering by one day in comparison with a higher flower set. The maximum span between the first flowering onset and the last one within all the progeny of the study was 11 days. With one exception, the incidence of the first flowering dates of all the progeny fluctuated only within four days - April 28 and May 1. Among them, the great majority of the seedlings had the first flowering date span of not more than ± one day. The progeny of Pink Lady × Discovery was the most exceptional having the mean day on the April 17 and the span about two days longer. The means of the first flowering dates within seedling populations and corresponding dates of both their parents and grandparents were very highly correlated (r = 0.926 for parents and r = 0.877 for grandparents). The incidence of flowering onset dates in almost all progeny followed a typical Gaussian distribution, in which the majority of seedlings start flowering within 3 or 4 days. The maximum difference between both extremes (found only in one progeny) was 11 days. The share of seedlings in both extremes was, however, very small - less than 2%. Keywords: apple breeding, flower set, Malus × domestica, progeny segregation, onset of flowering time Journal: Czech Journal of Genetics and Plant Breeding Pages: 23-28 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/221/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/221/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201501-0004.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:221-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Wenqi ZHOU Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: WU Zhongliang Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: Yingcong ZHANG Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: WU Dali Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: Duo LIU Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: Yuchuan WANG Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: Qingxiang GAO Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Name: Bingrong DANG Author-Workplace-Name: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, P.R. China Author-Name: LI Wenjian Author-Workplace-Name: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, P.R. China Author-Name: Suiwen HOU Author-Workplace-Name: Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China Author-Workplace-Name: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, P.R. China Title: Stable inheritance of excellent agricultural traits induced by 12C6+ heavy-ions in lentil (Lens culinaris Medik.) Abstract: The lentil (Lens culinaris Medik.) belongs to the most important leguminous plants in Asia. It is a very popular and highly nutritious food. However, small pod size and low yield limit its agricultural use. Through irradiation mutagenesis of dry lentil seeds by 12C6+ ion beam, we found some lines with excellent agricultural traits in M2 progeny at doses of 90 Gy (grey), such as height increase and improvement of the number of branches and three-seed pods as well as seeds with increased grain yield. Six generations of irradiated lentils were screened for these traits. In the M8 generation, the grain yield of three high-yielding lentil lines (lines hyl-1, hyl-2, and hyl-3) reached 212.4%, 195.3% and 190.8%, respectively, of the non-irradiated controls. The results indicated that crop improvement was stable inherited across the generations. Statistical analysis revealed that the increase in grain yield was due to increased total pod number and seeds per pod. Fortunately, adverse effects of irradiation (e.g. reduced germination rates and poor pollen vitality) disappeared over the eight generations. In conclusion, we present a practical method for the improvement of lentils through radiation breeding, leading to high yielding cultivars, which could support the use of this crop in agriculture. Keywords: 12C6+ irradiation, crop enhancement, high-yielding lentils, three-seed pods Journal: Czech Journal of Genetics and Plant Breeding Pages: 29-35 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/214/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/214/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201501-0005.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:214-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jelena M. ALEKSIĆ Author-Workplace-Name: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia Author-Name: Bojana BANOVIĆ Author-Workplace-Name: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia Author-Name: Jovanka MILJUŠ-ĐUKIĆ Author-Workplace-Name: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia Author-Name: Živko JOVANOVIĆ Author-Workplace-Name: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia Author-Name: Aleksandar MIKIĆ Author-Workplace-Name: Institute of Field and Vegetable Crops (IFVCNS), Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Branko ĆUPINA Author-Workplace-Name: Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Bojan ZLATKOVIĆ Author-Workplace-Name: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Niš, Serbia Author-Name: Snežana ANĐELKOVIĆ Author-Workplace-Name: Institute for Forage Crops (IFC), Kruševac, Serbia Author-Name: Ilaria SPANU Author-Workplace-Name: Institute of Biosciences and Bioresources (IBBR) UOS, Sesto Fiorentino, Florence, Italy Author-Name: Mihailo JELIĆ Author-Workplace-Name: Department of Genetics and Evolution, Faculty of Biology, University of Belgrade, Belgrade, Serbia Author-Name: Vesna MAKSIMOVIĆ Author-Workplace-Name: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia Title: A rapid and cost-effective procedure for delineation and utilization of genomic microsatellites for paralleled genotyping in Vicia faba Abstract: Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement. Keywords: faba bean, genomic SSRs, multiplexing, Type-it kit Journal: Czech Journal of Genetics and Plant Breeding Pages: 36-39 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/153/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/153/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201501-0006.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:153-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: R. Šafaříková Title: List of field crop varieties registered in the Czech Republic in 2014 Journal: Czech Journal of Genetics and Plant Breeding Pages: 40-41 Volume: 51 Issue: 1 Year: 2015 DOI: 10.17221/265/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/265/2014-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:265-2014-CJGPB