Template-Type: ReDIF-Article 1.0 Author-Name: QI Aiyong Author-Workplace-Name: College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: LI Xing Author-Workplace-Name: College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Lingzhi SHI Author-Workplace-Name: College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Daqun LIU Author-Workplace-Name: College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: LI Zaifeng Author-Workplace-Name: College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Title: Identification of a leaf rust resistance gene in the Chinese wheat line LB0288 Abstract: Leaf rust (LR), caused by Puccinia triticina (Pt), is worldwide one of the most spread diseases in common wheat (Triticum aestivum L.). With the objective of identifying and mapping new genes for resistance to leaf rust, F1 and F2 plants from a cross between the resistant cultivar LB0288 and the susceptible cultivar Thatcher were inoculated with the Chinese P. triticina pathotype THTT in the greenhouse. A set of 1273 simple sequence repeat (SSR) primer pairs were used to test the parents and the resistant and susceptible bulks. The results indicated, that LB0288 carried a single dominant resistance gene LrLB88, closely linked to the CAPS marker of Lr1 (WR003) and the SSR marker Xbarc144, with genetic distances of 0 cM and 5.3 cM, respectively. Based on the chromosomal location and seedling reactions, it can be concluded that LrLB88 might be a new leaf rust resistance gene. These markers can be useful for marker assisted selection in breeding of leaf rust resistant wheat cultivars. Keywords: leaf rust resistance gene, molecular mapping, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 43-49 Volume: 51 Issue: 2 Year: 2015 DOI: 10.17221/2/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/2/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201502-0001.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:2:id:2-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Imane MEDOUKALI Author-Workplace-Name: Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine, Constantine, Algérie Author-Name: Inès BELLIL Author-Workplace-Name: Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine, Constantine, Algérie Author-Name: Douadi KHELIFI Author-Workplace-Name: Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine, Constantine, Algérie Title: Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers Abstract: Genetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection site and morphological features was detected. The two isozyme systems analysed, esterase (EST) and glutamate oxaloacetate transaminase (GOT), proved polymorphic. Phenotype diversity of isozyme markers ranged from 0.07 to 0.61 with an average of 0.31 based on the polymorphic information content. The pairwise Jaccard's similarity coefficient ranged between 0.10 and 0.60, indicating that the collection represents genetically diverse species. A considerable number of species-specific zymograms were detected which can be used for the species identification. The clustering pattern of isozyme markers was incongruent with the groupings based on quantitative traits. The rich isozyme variability present among the Algerian clover species indicates that they can provide good gene resources for breeding. Keywords: clover, isozymes, morphology, PAGE, polymorphism Journal: Czech Journal of Genetics and Plant Breeding Pages: 50-61 Volume: 51 Issue: 2 Year: 2015 DOI: 10.17221/10/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/10/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201502-0002.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:2:id:10-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Katarína MITROVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Pavel SVOBODA Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jaroslava OVESNÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic Abstract: The onion (Allium cepa L.) constitutes an important part of the human diet and is of economic importance on the vegetable market. Methods that allow unambiguous differentiation of cultivars and breeding lines are required for market control and the protection of plant breeders' rights. DNA analyses, such as single sequence repeat polymorphism (SSRs), represent such a tool. Based on the evaluation of polymorphism in 21 SSR loci, we established a panel of 15 easy to score SSR markers that differentiated 16 commercial onion cultivars in the Czech Republic. The polymorphism ranged from 2-3 alleles per locus, indicating a low level of diversity among the cultivars. The panel can also be used to evaluate genetic resources. Keywords: Allium cepa L., diversity, genotyping, SSR markers, variety testing Journal: Czech Journal of Genetics and Plant Breeding Pages: 62-67 Volume: 51 Issue: 2 Year: 2015 DOI: 10.17221/16/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/16/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201502-0003.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:2:id:16-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Elmahdy Ibrahim METWALLY Author-Workplace-Name: Horticulture Department, Faculty of Agriculture, University of Kafrelsheikh, Kafr El-Sheikh, Egypt Author-Name: Mohamed Tawfik RAKHA Author-Workplace-Name: Horticulture Department, Faculty of Agriculture, University of Kafrelsheikh, Kafr El-Sheikh, Egypt Author-Workplace-Name: AVRDC - The World Vegetable Center, Shanhua, Tainan, Taiwan Title: Evaluation of selected Cucumis sativus accessions for resistance to Pseudoperonospora cubensis in Egypt Abstract: Downy mildew [Pseudoperonospora cubensis (Berk. & Curt.) Rostov] is a major destructive disease of cucumber (Cucumis sativus L.) worldwide. Resistant cultivars were recently introduced into Egypt, but yield losses were high if no fungicides were used. The objective of this study was to identify sources of resistance to downy mildew among Plant Introduction (PI) cucumber accessions from the U.S. National Plant Germplasm System. We evaluated 133 such accessions for downy mildew resistance under natural field epidemics during the summer 2013 at El-Beheira Governorate, Egypt. Mean ratings for downy mildew leaf damage ranged from 1 to 9 on a 0 to 9 scale. We classified 18 PI accessions (13.5%) as highly resistant (rating of 1.0-2.0), 46 (34.5%) as moderately resistant (rating 2.1-4.0), 40 (30%) as intermediate (rating 4.1-6.0), 12 (9%) as moderately susceptible (rating 6.1-7), and 17 (13%) as highly susceptible (rating > 7.1). The most resistant PI accessions were PI 432870, PI 432873, PI 432878, PI 432884, and PI 432886 with a rating of 1, which originated from China. The most susceptible PI accessions were Ames7736, PI 211979, PI 288991, PI 288992 and PI 289698 with a rating of 9. The five most resistant and five most susceptible accessions were further evaluated in replicated experiments during the summer 2014. Results from the repeated test confirmed the results from the first screening. No PI accession was found immune to downy mildew. However, high levels of resistance were observed in several PI accessions that could be useful for the breeding for resistance to P. cubensis in cucumber. Keywords: cucumber, cucurbit downy mildew, field resistance, germplasm Journal: Czech Journal of Genetics and Plant Breeding Pages: 68-74 Volume: 51 Issue: 2 Year: 2015 DOI: 10.17221/12/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/12/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201502-0004.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:2:id:12-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Pavel MAŘÍK Author-Workplace-Name: Plant Breeding Station, Lužany, SELGEN, a.s., Czech Republic Author-Name: Jana CHRPOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Ilja Tom PRÁŠIL Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Tibor SEDLÁČEK Author-Workplace-Name: Research Centre SELTON, Ltd., Stupice, Czech Republic Title: Six-row winter barley Lancelot Abstract: The Lancelot variety is a late to semi-late six-row feeding winter barley. It was developed at the Breeding Station, Lužany, SELGEN a.s. and registered in the Czech Republic in 2013. Lancelot has very good resistance to winter stresses in combination with resistance to BaMMV/BaYMV (Barley mild mosaic virus/Barley yellow mosaic virus) based on the gene rym4. Keywords: Barley mild mosaic virus, Barley yellow mosaic virus, cultivar description, Hordeum vulgare L., winter hardiness Journal: Czech Journal of Genetics and Plant Breeding Pages: 75-77 Volume: 51 Issue: 2 Year: 2015 DOI: 10.17221/61/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/61/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201502-0005.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:2:id:61-2015-CJGPB