Template-Type: ReDIF-Article 1.0 Author-Name: Jiazhen WANG Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: LI Zaifeng Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Lingzhi SHI Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Lin ZHU Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Zhikuan REN Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: LI Xing Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Daqun LIU Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Syed Jawad Ahmad SHAH Author-Workplace-Name: NIFA Tarnab, Peshawar, Pakistan Title: QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8 Abstract: The wheat cultivar Weimai 8 is a good source of adult-plant resistance to leaf rust in China. In order to understand the genetic background of the resistance genes, 179 F2:3 families derived from the cross Weimai 8 and Zhengzhou 5389 (susceptible to leaf rust) were used to construct a linkage map. Results showed that there was a major QTL on chromosome 2AS, temporarily named QLr.hbau-2AS, responsible for the resistance. QLr.hbau-2AS from the resistant parent Weimai 8 was between the SSR markers Xcfd36 and Xbarc1138, with an interval length of 2.58 cM. In the 2010-2011, 2011-2012 and 2012-2013 crop seasons, it explained 25.79, 71.55 and 60.72% of the phenotypic variation, respectively. The location of QLr.hbau-2AS was close to the leaf rust resistance gene Lr37. However, they may not be the same since QLr.hbau-2AS has a different virulence test response from Lr37. This study identified two closely linked flanking molecular markers QLr.hbau-2AS and these molecular markers will help marker-assisted selection in breeding resistant cultivars in the future. Keywords: gene postulation, molecular marker, QTL loci, wheat leaf rust Journal: Czech Journal of Genetics and Plant Breeding Pages: 79-85 Volume: 51 Issue: 3 Year: 2015 DOI: 10.17221/51/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/51/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201503-0001.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:3:id:51-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Václav ŠÍP Author-Workplace-Name: Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jana CHRPOVÁ Author-Workplace-Name: Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jana PALICOVÁ Author-Workplace-Name: Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Response of selected winter wheat cultivars to inoculation with different Mycosphaerella graminicola isolates Abstract: Five winter wheat cultivars, differing in resistance to Septoria tritici blotch (STB), were spray inoculated under field conditions for two years and at two locations with nine Mycosphaerella graminicola isolates and a mixture of isolates that were obtained from different regions of the Czech Republic. Main aims of this study were (i) to compare isolate, host cultivar and environmental effects on five traits indicative of STB severity and (ii) to analyse pathogen aggressiveness and host-pathogen relations for improving evaluation of cultivar resistance. ANOVA showed in all traits, except the reduction in 1000 grain weight, significant isolate effects. However, the effects of isolate and genotype by isolate interactions were much lower (2.3-4%) than cultivar (19.1-53.7%) and environmental (11.9-58.6%) effects. Cultivar resistance to the disease limited much the loss in grain weight per spike to a halve, from 34.7% in the susceptible Bakfis to 17.3% in the resistant Arina. Visual scoring of symptoms in the middle and at the end of disease development (performed on the 1-9 scale), reflecting the disease progress and infected leaf area, showed the highest cultivar effect (54%) and could be recommended for evaluation of cultivar resistance in breeding practice. All examined traits were significantly interrelated, but significant differences between all the five cultivars were only detected after examination of the % coverage of flag leaves with lesions bearing pycnidia. Resistance in the cultivar Arina was detected by all isolates and the isolate mixture. In spite of significant differences in classification of resistances in the cultivars Bohemia and Mulan after inoculation with one isolate (1081), specific interactions between cultivars and isolates collected in this Central European region are rare. The study leads to a conclusion that investigation into stability of STB resistance across a wide range of environments is more valuable for breeding purposes than the study of cultivar response to different isolates. Combination of important isolate properties in a mixture of isolates is stressed as well. Keywords: breeding for resistance, methodology of tests, Septoria tritici blotch, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 86-95 Volume: 51 Issue: 3 Year: 2015 DOI: 10.17221/44/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/44/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201503-0002.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:3:id:44-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Meena KANTI Author-Workplace-Name: Central Research Institute for Jute and Allied Fibres, Barrackpore, India Author-Name: Kammili ANJANI Author-Workplace-Name: Directorate of Oilseeds Research, Hyderabad, India Author-Name: Betha USHA KIRAN Author-Workplace-Name: Directorate of Oilseeds Research, Hyderabad, India Author-Name: K. VIVEKANANDA Author-Workplace-Name: Directorate of Oilseeds Research, Hyderabad, India Title: Agro-morphological and molecular diversity in castor (Ricinus communis L.) germplasm collected from Andaman and Nicobar Islands, India Abstract: Castor (Ricinus communis L.) is an industrial oilseed crop grown worldwide. Its oil with more than 80% ricinoleic acid makes it a chief raw material for numerous industrial applications and biofuel production. Castor grows in a wild form across India including Andaman & Nicobar Islands, which are geographically quite isolated from mainland India. Thirty-three accessions growing in isolation in these Islands were used in the present study. Genetic diversity among these accessions was assessed using 18 agro-morphological traits and 29 EST-SSR markers. High agro-morphological and molecular variability was observed among these accessions. Both agro-morphological traits and EST-SSR markers effectively discriminated the accessions. However, EST-SSRs separated the accessions into more groups than did agro-morphological data, implying high efficiency and resolution of EST-SSR markers in genetic analysis of castor germplasm from Andaman & Nicobar. The diverse accessions identified in the present investigation would serve as genetically diverse sources in castor breeding programmes. Keywords: agro-morphological traits, EST-SSRs, genetic diversity, polymorphism Journal: Czech Journal of Genetics and Plant Breeding Pages: 96-109 Volume: 51 Issue: 3 Year: 2015 DOI: 10.17221/205/2014-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/205/2014-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201503-0003.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:3:id:205-2014-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Chao-Wen SHE Author-Workplace-Name: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, P.R. China Author-Workplace-Name: Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua University, Huaihua, Hunan, P.R. China Author-Workplace-Name: Department of Life Sciences, Huaihua University, Huaihua, Hunan, P.R. China Author-Name: Xiang-Hui JIANG Author-Workplace-Name: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, P.R. China Author-Workplace-Name: Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua University, Huaihua, Hunan, P.R. China Author-Workplace-Name: Department of Life Sciences, Huaihua University, Huaihua, Hunan, P.R. China Title: Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes Abstract: The mitotic chromosomes of Lablab purpureus (L.) Sweet were characterised using sequential combined propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) (CPD) staining and fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. The detailed karyotype of this species was established using prometaphase chromosomes. After CPD staining, CPD and DAPI+ bands were shown simultaneously. CPD bands occurred in the proximal regions of the long arms of all chromosome pairs and at all 45S rDNA sites, while the DAPI+ bands appeared in all centromeres. FISH with rDNA probes revealed one 5S locus and eight 45S loci. The single 5S locus was interstitially located on the long arms of the shortest chromosome pair. Among the 45S loci, two large loci were located in the secondary constrictions of the short arms of two chromosome pairs; six small or minimal loci were proximally located on the short or long arms of six chromosome pairs. Each prometaphase chromosome pair could be identified using the CPD and DAPI+ bands, the rDNA-FISH signals in combination with the chromosome measurements and condensation patterns. The karyotype was formulated as 2n = 2x = 22 = 14m (2SAT) + 6sm + 2st (2SAT), and the asymmetry indices, CI, A1, A2, As K%, AI and the Stebbins category were 38.23 ± 7.06, 0.36, 0.31, 61.99, 5.68 and 2B, respectively. Keywords: combined PI and DAPI staining, FISH, hyacinth bean, karyotyping, ribosomal gene Journal: Czech Journal of Genetics and Plant Breeding Pages: 110-116 Volume: 51 Issue: 3 Year: 2015 DOI: 10.17221/32/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/32/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201503-0004.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:3:id:32-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jose Alberto OLIVEIRA PRENDES Author-Workplace-Name: Plant Production Area, Department of Organisms and Systems Biology, Mieres Polytechnic School, University of Oviedo, Mieres, Asturias, Spain Author-Name: Pedro PALENCIA Author-Workplace-Name: Plant Production Area, Department of Organisms and Systems Biology, Mieres Polytechnic School, University of Oviedo, Mieres, Asturias, Spain Title: Morphological characterization and turf performance of Paula hard fescue and Casero colonial bentgrass selections under low maintenance conditions Abstract: Planting grasses that require low maintenance is a good option for reducing the management input required. The objective of this study was to obtain information about turfgrass characteristics based on spaced-plant characterization and turf performance in turf plots of two selections (Paula hard fescue and Casero colonial bentgrass) recently released for public distribution by the Plant Production Area of the University of Oviedo. The plants were grown under conditions of no irrigation, no fertilizer, no pesticide application and minimal mowing over a 3-year period (2012-2014). The following morphological measurements were done in a spaced-plant nursery: heading date, plant height, inflorescence length and flag leaf length and width. The turf plots were evaluated for several traits including overall turfgrass quality (turf score), leaf texture and genetic colour. In both the spaced-plant nursery and turf trials, hard fescue genotypes displayed better morphological characteristics (narrow leaves, reduced plant height) and performed better (overall high turfgrass quality, fine leaf texture, dark green colour) than bentgrass genotypes under conditions of low maintenance. For areas in which climate, soil characteristics and management conditions are similar to those encountered in this trial, the selection of Paula hard fescue is recommended for use in low-maintenance turf settings because of the good turfgrass performance and morphological characteristics of this selection. Keywords: Agrostis, Festuca, spaced-plant nursery, turf plots Journal: Czech Journal of Genetics and Plant Breeding Pages: 117-122 Volume: 51 Issue: 3 Year: 2015 DOI: 10.17221/78/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/78/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201503-0005.txt Handle: RePEc:caa:jnlcjg:v:51:y:2015:i:3:id:78-2015-CJGPB