Template-Type: ReDIF-Article 1.0 Author-Name: Lingzhi SHI Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: LI Zaifeng Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Xiaodong WANG Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Zhanhai KANG Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Lin ZHU Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Zhikuan REN Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: LI Xing Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Daqun LIU Author-Workplace-Name: College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China Title: Genetic analysis and molecular mapping of a leaf rust resistance gene in the wheat line 19HRWSN-129 Abstract: The wheat (Triticum aestivum L.) line 19HRWSN-129 from CIMMYT showed resistance to the major Puccinia triticina (Pt) races collected in China. A 144 F2:3 population was generated by crossing the resistant line 19HRWSN-129 with the susceptible line Zhengzhou5389. All the F2:3 plants were phenotyped by inoculating with the Pt race PHGN in the greenhouse. Segregation analysis indicated the presence of a single dominant gene conferring resistance to leaf rust in the wheat line 19HRWSN-129, temporarily designated as Lrshi. Using molecular markers, we mapped this resistance locus on chromosome 7BL. It was closely linked to the SSR markers Xgwm344 and Xcfa2040 with genetic distances of 2.8 cM and 1.4 cM, respectively. Compared with other chromosome 7BL-located leaf rust resistance genes (Lr14a, Lr14b, Lr68), we demonstrate, that Lrshi is a novel wheat leaf rust resistance gene. Keywords: gene mapping, molecular marker, SSR, wheat leaf rust Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-5 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/167/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/167/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201601-0001.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:167-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Maneesh MISHRA Author-Workplace-Name: Central Institute for Subtropical Horticulture, Indian Council of Agricultural Research (ICAR), Lucknow, India Author-Name: Syed Uzma JALIL Author-Workplace-Name: Central Institute for Subtropical Horticulture, Indian Council of Agricultural Research (ICAR), Lucknow, India Author-Name: Rupesh Kumar MISHRA Author-Workplace-Name: Central Institute for Subtropical Horticulture, Indian Council of Agricultural Research (ICAR), Lucknow, India Author-Name: Swati KUMARI Author-Workplace-Name: Central Institute for Subtropical Horticulture, Indian Council of Agricultural Research (ICAR), Lucknow, India Author-Name: Brajesh Kumar PANDEY Author-Workplace-Name: Horticulture Science Division, Indian Council of Agricultural Research (ICAR), New Delhi, India Title: In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii Abstract: Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed by cultural as well as PCR analysis using species-specific primers. The roots of transgenic plants were wounded to facilitate the entry of the pathogen. The histopathological analysis revealed the presence of mycelium in vascular bundles. However, none of the plants showed symptoms of wilt disease during the investigation. In vitro pathogen inhibition assay and subsequently spore germination assay revealed that the crude leaf extract of transformed plants inhibited the germination of fungal conidia. The leaf tissue studied for expression of endochitinase revealed that two transgenic plants showed very high activity of N-acetyl-d-glucosamine (0.741 and 0.738 µM/min/µg of protein, respectively) which clearly indicated that transgenic plants could not develop any symptoms of wilt disease due to overexpression of endochitinase. Keywords: chitinase, genetic transformation, pathogen inhibition assay, quantification of chitinase, wilt disease Journal: Czech Journal of Genetics and Plant Breeding Pages: 6-13 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/74/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/74/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201601-0002.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:74-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Fei-Yu TANG Author-Workplace-Name: Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, College of Agronomy, Ministry of Education, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: MO Wang-Cheng Author-Workplace-Name: Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, College of Agronomy, Ministry of Education, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Wen-Jun XIAO Author-Workplace-Name: Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, College of Agronomy, Ministry of Education, Jiangxi Agricultural University, Nanchang, P.R. China Title: Genetic effects of high fibre strength breeding lines in crosses with transgenic Bt cotton cultivars (Gossypium hirsutum L.) Abstract: Evaluation of genetic effects in cotton (Gossypium hirsutum L.) high fibre strength lines is necessary for the genetic improvement of fibre properties. Six breeding lines with high fibre strength and five transgenic Bt cultivars were diallely crossed. Fibre properties and agronomic traits of 11 parents and resultant 30 F1 hybrids were analyzed by an additive-dominant model with genotype by environment interaction effects. Lint percentage, seed index, fibre length, strength and micronaire were primarily controlled by additive effects. Lint yield was mainly governed by dominance effects. Boll size was equally influenced by additive and dominance effects. A9-1 was a desirable general combiner for lint percentage, micronaire and fibre length. Yumian1 and Jinxing2 were good general combiners for fibre strength. Gk22 was a desirable general combiner for lint yield and boll size but poor for fibre length and strength. Some F1 hybrids were identified with favourable heterozygous dominant effects for lint yield and various fibre properties. This study revealed that current transgenic Bt cotton cultivars can be improved in fibre quality and lint yield by using some of the lines with high fibre strength in crosses with them. Keywords: additive dominant model, fibre quality, heterosis, lint yield Journal: Czech Journal of Genetics and Plant Breeding Pages: 14-21 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/116/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/116/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201601-0003.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:116-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Marek SEIDENGLANZ Author-Workplace-Name: Department of Plant Protection, AGRITEC Research, Breeding and Services, Ltd., Šumperk, Czech Republic Author-Name: Igor HUŇADY Author-Workplace-Name: Department of Grain Legumes and Technical Crops, Agritec Plant Research Ltd., Šumperk, Czech Republic Title: Effects of faba bean (Vicia faba) varieties on the development of Bruchus rufimanus Abstract: The development of Bruchus rufimanus was assessed in randomised field trials from 2005 to 2007 with eight faba bean genotypes (SU 5/13, SU-BT, Mistral, Merkur, Stabil Merlin, Melodie, Divine). Significant differences, reproducible between the years, were found between the faba bean lines. The varieties most attractive to B. rufimanus ovipositing females were SU-BT and Divine. Merkur showed a certain level of non-preference. The highest mortality rates of eggs and first instar larvae in pod valve tissues were recorded in Merkur (34-45%) and Divine (51-55%). The highest mortality rates of larvae and pupae during their development in seeds (effect of seed coat and cotyledon tissues) were recorded in Merkur (87-90%) and Melodie (87-99%). Parasitation by Triaspis thoracicus contributed to this mortality. The highest levels of B. rufimanus parasitation were found in SU-BT (32-35%), the lowest in Melodie (3-9%). The total reduction in Bruchus rufimanus individuals during their development was relatively high in all varieties, ranging from 72% to 99%. Keywords: antibiosis, Bruchus rufimanus mortality in seeds, nonpreference, plant resistance to bruchids Journal: Czech Journal of Genetics and Plant Breeding Pages: 22-29 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/122/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/122/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201601-0004.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:122-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tibor SEDLÁČEK Author-Workplace-Name: Research Centre SELTON, Ltd., Stupice, Czech Republic Author-Name: Pavel MAŘÍK Author-Workplace-Name: Research Centre SELTON, Ltd., Stupice, Czech Republic Author-Name: Jana CHRPOVÁ Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Identification of genes conferring resistance to viral diseases of barley using multiplex PCR Abstract: This paper describes a rapid and cost-effective assay for identifying economically important barley resistance genes based on multiplex PCR. A newly created reaction mixture is used for detecting the resistance genes rym4, rym5, rym11-b and Ryd2 conferring resistance to barley yellow mosaic viruses and Barley yellow dwarf virus (BYDV). It is possible to identify all these genes using only a single PCR reaction without the necessity for subsequent cutting with restriction endonucleases. This is important for developing resistant barley genotypes inasmuch as it can increase work productivity and reduce costs. Keywords: BaYMV, BaMMV, BaYMV-2, BYDV, Hordeum, resistance genes Journal: Czech Journal of Genetics and Plant Breeding Pages: 30-33 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/171/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/171/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201601-0005.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:171-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Qin ZHANG Author-Workplace-Name: College of Landscape Architecture, Beijing Forestry University, Beijing, P.R. China Author-Workplace-Name: College of Landscape and Travel and Author-Name: Fan ZHANG Author-Workplace-Name: College of Landscape and Travel and Author-Name: LI Baohui Author-Workplace-Name: College of Forestry, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Luosha ZHANG Author-Workplace-Name: College of Forestry, Agricultural University of Hebei, Baoding, P.R. China Author-Name: Huiqin SHI Author-Workplace-Name: College of Landscape and Travel and Title: Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine Abstract: Trollius chinensis Bunge is one of the important ornamental and medicinal plants cultivated in China. In this study, the tetraploid plants of Trollius chinensis were successfully induced by colchicine and their morphological, cytological and fertility characteristics were investigated. The results indicated that the tetraploid species of Trollius chinensis (2n = 4x = 32) was efficiently induced from the diploid species (2n = 2x = 16) by colchicine under 0.05% to 0.2% concentrations, with the best induction under treatment combinations of 0.1%, 24h duration and 0.2%, 12h duration. In comparison with the diploid plants, the tetraploid plants exhibited dramatically varied phenotypic features, showing increased leaf length and width, petiole, flower and pollen diameters. In addition, they also exhibited increased stomatal size and chloroplast numbers in guard cells. Tetraploidy reduced the pollen fertility. Nevertheless, the maintenance of female fertility makes the tetraploid a potential germplasm for further polyploid breeding. Our investigation provides a potential design for an effective pathway to generate varieties of this plant species with chromosome doubling. Keywords: morphological, cytological and fertility characteristics, polyploidy induction, Trollius chinensis Journal: Czech Journal of Genetics and Plant Breeding Pages: 34-38 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/89/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/89/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201601-0006.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:89-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: R. Šafaříková Title: List of field crop varieties registered in the Czech Republic in 2015 Journal: Czech Journal of Genetics and Plant Breeding Pages: 39-40 Volume: 52 Issue: 1 Year: 2016 DOI: 10.17221/4/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/4/2016-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:4-2016-CJGPB