Template-Type: ReDIF-Article 1.0 Author-Name: Barbara Ferrari Author-Workplace-Name: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy Author-Name: Massimo Romani Author-Workplace-Name: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy Author-Name: Gregoire Aubert Author-Workplace-Name: Institut National de la Recherche Agronomique, Unité Mixte de Recherches en Agroécologie, Dijon, France Author-Name: Karen Boucherot Author-Workplace-Name: Institut National de la Recherche Agronomique, Unité Mixte de Recherches en Agroécologie, Dijon, France Author-Name: Judith Burstin Author-Workplace-Name: Institut National de la Recherche Agronomique, Unité Mixte de Recherches en Agroécologie, Dijon, France Author-Name: Luciano Pecetti Author-Workplace-Name: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy Author-Name: Myriam Huart-Naudet Author-Workplace-Name: Institut National de la Recherche Agronomique, Unité Mixte de Recherches en Agroécologie, Dijon, France Author-Name: Anthony Klein Author-Workplace-Name: Institut National de la Recherche Agronomique, Unité Mixte de Recherches en Agroécologie, Dijon, France Author-Name: Paolo Annicchiarico Author-Workplace-Name: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy Title: Association of SNP markers with agronomic and quality traits of field pea in Italy Abstract: Only a few studies on pea (Pisum sativum) investigated the association of single nucleotide polymorphisms (SNP) markers with key agronomic traits. This study aimed to explore the association of a standard set of 384 SNP with grain yield, seed protein content, seed weight, onset of flowering, plant height and lodging susceptibility, in three connected bi-parental recombinant inbred line (RIL) populations including 90 lines each. These RIL originated from crosses between three cultivars that displayed high and stable grain yield across Italian environments, namely, Attika (A), Isard (I), and Kaspa (K). The 270 lines were phenotyped in a spring-sown environment of Lodi (northern Italy; 45°19'N, 9°30'E). Variation among lines within the populations was significant (P < 0.01) in all cases except lodging susceptibility in one cross and, when expressed in terms of the genetic coefficient of variation, proved moderately large for most traits (including grain yield and seed protein content). Overall, we detected six quantitative trait loci (QTL) in the A × I linkage map, eight QTL in K × A, and nine QTL in K × I. Among them, there were three QTL in K × A and two QTL in K × I for grain yield, and one QTL in A × I and two QTL in both K × A and K × I for seed protein content. The consensus map, which included 130 markers (covering about 1094 cM), retained one QTL for grain yield and one for flowering time that co-located on LGII, and three for seed weight on LGIII, LGVI and LGVII. The QTL co-locating for yield and flowering time explained 8% and 31% of the overall phenotypic variation, respectively, for the two traits, and could be exploited in marker-assisted selection for adaptation to the target region. Keywords: grain yield, marker-assisted selection, Pisum sativum, protein content, QTL Journal: Czech Journal of Genetics and Plant Breeding Pages: 83-93 Volume: 52 Issue: 3 Year: 2016 DOI: 10.17221/22/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/22/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201603-0001.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:22-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Saber Delpasand Khabbazi Author-Workplace-Name: TARBIYOTEK Laboratories, Department of Field Crops, Faculty of Agriculture, University of Ankara, Dişkapi-Ankara, Turkey Author-Name: Allah Bakhsh Author-Workplace-Name: Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Niğde University, Niğde, Turkey Author-Name: CengİZ Sancak Author-Workplace-Name: TARBIYOTEK Laboratories, Department of Field Crops, Faculty of Agriculture, University of Ankara, Dişkapi-Ankara, Turkey Author-Name: Sebahattin Özcan Author-Workplace-Name: TARBIYOTEK Laboratories, Department of Field Crops, Faculty of Agriculture, University of Ankara, Dişkapi-Ankara, Turkey Title: Molecular characterization of snowdrop lectin (GNA) and its comparison with reported lectin sequences of Amaryllidaceae Abstract: Plant lectins have become efficient sources of insect resistance in crops. The present study was conducted to identify, amplify, clone and characterize the plant lectin gene GNA. The lectin, present in Galanthus nivalis (snowdrop), is an agglutinin toxic to hemiptera. The attempt was made to elucidate the relationship of the lectin gene trGNA (GNA isolated and characterized from Turkey) with other previously cloned lectins having insecticidal activity and to ensure the presence of the conserved mannose-binding region/site in the gene sequence. The full-length cDNA of trGNA was 477 bp that contained a 333 bp open reading frame encoding 157 amino acid proteins with 23 amino acids of signal peptide. BLAST results showed that trGNA has 89-97% similarity with previously reported GNA sequences while it has 84-96% similarity with earlier reported GNA protein sequences. No intron was detected within the region of genomic sequence corresponding to trGNA full-length cDNA. According to the search results from the NCBI (National Centrer for Biotechnology Information database), trGNA from Galanthus nivalis is most similar to the previously reported lectin sequences of Narcissus tazetta with a similarity percentage of 87%. The obtained results are useful for engineering of plants with enhanced insecticidal activity against chewing and sucking insects, causing crop pests. In addition, medical application of lectins may also be considered. Keywords: bioinformatics, biotic stress, Galanthus nivalis, gene cloning, gene sequence, insecticidal activity Journal: Czech Journal of Genetics and Plant Breeding Pages: 94-100 Volume: 52 Issue: 3 Year: 2016 DOI: 10.17221/58/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/58/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201603-0002.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:58-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Sixue LIU Author-Workplace-Name: College of Life Sciences, Capital Normal University, Beijing, P.R. China Author-Workplace-Name: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, P.R.China Author-Name: Limeng DENG Author-Workplace-Name: College of Life Sciences, Capital Normal University, Beijing, P.R. China Author-Name: FU Yaping Author-Workplace-Name: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, P.R.China Author-Name: HU Guocheng Author-Workplace-Name: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, P.R.China Author-Name: Wenzhen LIU Author-Workplace-Name: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, P.R.China Author-Name: Xin ZHAO Author-Workplace-Name: College of Life Sciences, Capital Normal University, Beijing, P.R. China Title: Identification and characterization of the yls mutation in rice (Oryza sativa L.) with lower photosynthetic pigment content Abstract: Normal chloroplast development in rice is essential for photosynthesis and yield potential. To explore the physiological and molecular mechanism of chloroplast development, we isolated the rice mutant yls, which has yellow-green leaves at the rice seedling stage. In comparison with wild type (WT) plants, mutant plants had lower chlorophyll and carotenoid contents at the seedling stage. Transmission electron micrographs of the leaves of mutant plants showed abnormal grana stacking. We finally mapped the YLS gene within the BAC clone OSJNBa0032M21 of chromosome 11. Sequence analysis revealed the existence of a 33-bp deletion within the 3'-untranslated region (UTR) of the cpSRP54 gene, which encodes the 54-kDa subunit of the chloroplast signal recognition particle (SRP). A knockdown of cpSRP54 using RNAi technology produced the yls phenotypes, indicating that cpSRP54 is responsible for the phenotypic changes found in the yls mutant. The study suggests the existence of a functional association between cpSRP54 and chloroplast development in rice. Keywords: chloroplast development, map-based cloning, SRP54 protein, yellow-green leaf mutant Journal: Czech Journal of Genetics and Plant Breeding Pages: 101-107 Volume: 52 Issue: 3 Year: 2016 DOI: 10.17221/42/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/42/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201603-0003.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:42-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Veronika DUMALASOVÁ Author-Workplace-Name: Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Pavel BARTOŠ Author-Workplace-Name: Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Reaction of wheat to common bunt and dwarf bunt and reaction of triticale to dwarf bunt Abstract: Resistance to common bunt and to dwarf bunt in winter wheat cultivars recently registered in the Czech Republic was studied in artificially inoculated field trials in the years 2013-2015. In trials with common bunt, seeds of each experimental series were inoculated with a different mixture of isolates. In dwarf bunt trials, the soil surface was inoculated with a natural bunt population from a single locality. Several selected unregistered wheat cultivars, triticale cultivars and cultivars/lines known as sources of dwarf bunt resistance were also included in the trials with dwarf bunt. Out of the recently registered winter wheat cultivars only cv. Genius showed resistance to common bunt in both test years. Cv. Sailor was highly resistant to common bunt only in one trial, but not in other trials with different inoculum. Cv. Saturnus and the registered cv. Potenzial showed the lowest incidence of dwarf bunt in both years. The triticale cultivars were highly resistant to dwarf bunt compared to the wheat cultivars used as checks. High resistance to dwarf bunt in the tested sources of resistance was confirmed. Keywords: bunt incidence, resistance, sources of resistance, winter wheat cultivars Journal: Czech Journal of Genetics and Plant Breeding Pages: 108-113 Volume: 52 Issue: 3 Year: 2016 DOI: 10.17221/48/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/48/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201603-0004.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:48-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Youru WANG Author-Workplace-Name: Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, P.R. China Author-Workplace-Name: Life Science College, Hubei Normal University, Huangshi, Hubei, P.R. China Title: Callus induction and frond regeneration in Spirodela polyrhiza Abstract: Spirodela polyrhiza belongs to the family Lemnaceae (duckweed), which is a group of small aquatic plants offering an attractive plant expression system for the production of recombinant protein. No frond regeneration protocol has been established in this species yet. An efficient protocol for plant regeneration through organogenesis has been developed in Spirodela polyrhiza for the first time. Calli were successfully induced from 92% of explants on Murashige and Skoog (MS) medium with 10 μM naphthaleneacetic acid, 2 μM thidiazuron, 1μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 3% sucrose. MS medium containing 1% (m/v) sorbitol and 1 µM 2,4-D supported long lasting growth (at least 5 months) of 98% of calli. Plants regenerated from 92% of calli on Schenk and Hildebrandt (SH) medium with 10 μM zeatin and 1% (m/v) sucrose. The protocol for frond regeneration could be a good basis for transgenic engineering of S. polyrhiza. Keywords: duckweed, regeneration, tissue culture Journal: Czech Journal of Genetics and Plant Breeding Pages: 114-119 Volume: 52 Issue: 3 Year: 2016 DOI: 10.17221/134/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/134/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201603-0005.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:134-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Meha SHARMA Author-Workplace-Name: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Satinder KAUR Author-Workplace-Name: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Manny SALUJA Author-Workplace-Name: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Parveen CHHUNEJA Author-Workplace-Name: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India Title: Mapping and characterization of powdery mildew resistance gene in synthetic wheat Abstract: Powdery mildew caused by Blumeria graminis f.sp. tritici is significantly affecting wheat production worldwide. In the search for new sources of resistance we investigated the powdery mildew resistance in the synthetic hexaploid wheat line "Synthetic 43" with its D genome from Aegilops tauschii. This line was developed at CIMMYT and resists a number of common bread wheat diseases. The line was crossed with the powdery mildew susceptible hexaploid wheat cultivar WH542 and a mapping population consisting of 148 RILs was developed. Inheritance studies in the RIL population revealed monogenic inheritance of powdery mildew resistance both at the seedling stage and adult plant stage. This resistance gene was mapped at a distance of 4.8 cM from SSR marker Xwmc150 on chromosome 7D and has been temporarily designated as PmT. Keywords: Aegilops tauschii, Blumeria graminis, SSR, Triticum aestivum Journal: Czech Journal of Genetics and Plant Breeding Pages: 120-123 Volume: 52 Issue: 3 Year: 2016 DOI: 10.17221/187/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/187/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201603-0006.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:3:id:187-2015-CJGPB