Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 52, AUTHORS INDEX Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/9346-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/9346-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:9346-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jana DLUHOŠOVÁ Author-Workplace-Name: Department of Experimental Biology, Masaryk University, Brno, Czech Republic Author-Name: Jana ŘEPKOVÁ Author-Workplace-Name: Department of Experimental Biology, Masaryk University, Brno, Czech Republic Author-Name: Hana JAKEŠOVÁ Author-Workplace-Name: Dr. Hana Jakesova Clovers and Grass Plant Breeding, Hladké Životice, Czech Republic Author-Name: Jan NEDĚLNÍK Author-Workplace-Name: Agricultural Research, Ltd., Troubsko, Czech Republic Title: Impact of interspecific hybridization of T. pratense × T. medium and backcrossing on genetic variability of progeny Abstract: Red clover (Trifolium pratense L.) is a high-quality fodder crop which has been hybridized successfully with its wild relative zigzag clover (T. medium L.). The aim of this study was to evaluate the genetic impact of interspecific hybridization and subsequent repeated backcrossing on the variability within hybrid progeny genomes. Nuclear DNA content of 800 and 753 hybrid plants from F7/F8 and F8/F9 generations, respectively, was measured by flow cytometry. Resulting values were converted to estimated chromosome counts, which were successfully validated on a sample of 28 plants by counting mitotic chromosomes. The two generations showed a similar distribution of various chromosome counts ranging from 22 to 47 chromosomes. In total, 24.0% and 34.3% of plants from the two generations had different numbers of chromosomes from their parental plants. Variability within the hybrid population was assessed by fluorescent in situ hybridization using rDNA probes. Individual plants had a pattern of 5S and 45S rDNA loci rather more similar to that of T. pratense than of T. medium. Numbers of chromosomes with clusters of 5S rDNA ranged from 6 to 14 while those of 45S rDNA varied between 4 and 13. Individual arrangements were almost unique, and some plants possessed also novel formations which were not present in any of the parental species, such as a cluster of 5S rDNA surrounded by 45S rDNA clusters or a 45S rDNA cluster surrounded by 5S rDNA clusters. This suggests complex rearrangements connected with post-hybridization stabilization of hybrid genomes. Keywords: cytology, FISH, hybrids, rDNA, red clover, zigzag clover Journal: Czech Journal of Genetics and Plant Breeding Pages: 125-131 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/115/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/115/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201604-0002.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:115-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Maria SURMA Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Tadeusz ADAMSKI Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Halina WIŚNIEWSKA Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Zygmunt KACZMAREK Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Iwona MEJZA Author-Workplace-Name: Poznań University of Life Sciences, Poznań, Poland Author-Name: Stanisław MEJZA Author-Workplace-Name: Poznań University of Life Sciences, Poznań, Poland Author-Name: Anetta KUCZYŃSKA Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Karolina KRYSTKOWIAK Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Krzysztof MIKOŁAJCZAK Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Author-Name: Piotr OGRODOWICZ Author-Workplace-Name: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland Title: Uni- and multivariate approaches to evaluating the susceptibility of wheat hybrids to Fusarium head blight Abstract: Fusarium head blight (FHB) is a disease of small grain cereals caused by Fusarium species. The pathogens affect spikes and kernels, resulting in reductions of yield and its quality. The present study was conducted to evaluate variation in the FHB resistance of wheat F2 hybrids derived from 16 crosses between winter wheat cultivars of various origin and with different susceptibility to FHB. Plants were inoculated with a conidial suspension consisting of a mixture of F. culmorum, F. graminearum and F. avenaceum isolates. After harvest 1000-kernel weight, number of kernels per spike and kernel weight per spike were evaluated in inoculated and control plants. Disease symptoms were observed on kernels of infected plants and the percentage of Fusarium-damaged kernels (FDK) was calculated. The data were statistically evaluated using uni- and multivariate analyses. A significant influence of genotype and treatment on all observed characteristics was detected. Contrasts between control and inoculated plants showed that inoculation lowered the mean values of all the yield-related traits significantly (in the statistical sense). Results of uni- and multivariate analyses enabled us to find three cross combinations which exhibited a low FDK percentage and simultaneously a relatively low reduction of 1000-kernel weight after inoculation. They may be promising for breeding wheat with improved resistance to FHB. Keywords: ANOVA, kernel damage, MANOVA, resistance to Fusarium head blight, winter wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 132-138 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/86/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/86/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201604-0003.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:86-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Yanjie JIANG Author-Workplace-Name: Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P.R. China Author-Name: Fangfang ZHU Author-Workplace-Name: Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P.R. China Author-Name: Shibin CAI Author-Workplace-Name: Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P.R. China Author-Name: WU Jizhong Author-Workplace-Name: Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P.R. China Author-Name: Qiaofeng ZHANG Author-Workplace-Name: Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P.R. China Title: Quantitative trait loci for resistance to Sharp Eyespot (Rhizoctonia cerealis) in recombinant inbred wheat lines from the cross Niavt 14 × Xuzhou 25 Abstract: This paper studies QTL (quantitative trait locus) resistance to Sharp Eyespot in wheat using a genetic population of the recombinant inbred line (RIL) hybridized from Niavt 14 and Xuzhou 25. Based on three-year phenotypic data and a resultant RIL genetic map, three QTLs in total associated with Sharp Eyespot resistance were detected. Two out of the three loci were detected on chromosome 2B, namely Qses.jaas-2b1 and Qses.jaas-2b2, of which Qses.jaas-2b1 accounted for 5.46 and 8.56% of the phenotypic variation in the field after inoculation in two successive years and Qses.jaas-2b2 accounted for 6.04, 8.10 and 12.92% after field inoculation for three successive years. A further QTL for resistance gene named Qses.jaas-7d was detected on chromosome 7D in addition to the two on 2B and this exceeding 11.25% of the phenotypic variation. These results indicate that QTLs associated with Sharp Eyespot possibly exist on the linkage groups of chromosomes 2B and 7D in wheat and lay the foundation for further research of QTL resistances to Sharp Eyespot in wheat. Keywords: genetic map, molecular marker, QTL, Rhizoctonia cerealis, Sharp Eyespot, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 139-144 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/74/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/74/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201604-0004.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:74-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Farnaz ABED-ASHTIANI Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Name: Jugah KADIR Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Name: Abbas NASEHI Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Name: Seyed-Reza HASHEMIAN-RAHAGHI Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Name: Ganesan VADAMALAI Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Workplace-Name: Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Name: Siti-Khadijah RAMBE Author-Workplace-Name: Laboratory of Bioresource Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Title: Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia. Abstract: The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysia, including the five states of Selangor, Penang, Kedah, Kelantan, and Perak between 2010 and 2014. DNA sequence analyses of the internal transcribed spacer (ITS), actin, β-tubulin and calmodulin gene regions, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses were conducted to analyse 35 Magnaporthe isolates. Phylogenetic analysis of the combined dataset confirmed the identification of all isolates as M. oryzae with a high distance from other Magnaporthe (Pyricularia) species. RAPD and ISSR analyses indicated the existence of a relatively low similarity index value among M. oryzae isolates through identification of four main clades. The clustering of RAPD and ISSR analyses demonstrated that there was a correlation between the isolates and their geographical origins. Keywords: genetic diversity, ISSR, Oryza sativa, RAPD, rDNA-ITS, rice blast Journal: Czech Journal of Genetics and Plant Breeding Pages: 145-156 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/102/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/102/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201604-0005.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:102-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Nadjat BENKHERBACHE Author-Workplace-Name: Departement of Agricultural Sciences, University of Mohamed Boudiaf - M'sila, M'sila, Algeria Author-Name: Alessandro TONDELLI Author-Workplace-Name: Genomics Research Centre, Fiorenzuola d'Arda, Italy Author-Name: Abdelhamid DJEKOUNE Author-Workplace-Name: Departement of Biology, University of Constantine, Constantine, Algeria Author-Name: Enrico FRANCIA Author-Workplace-Name: University of Reggio Emilia, Modena, Italy Author-Name: Nicola PECCHIONI Author-Workplace-Name: National Institute of Cereal Genetic, Foggia, Italy Author-Name: Liamine HASSOUS Author-Workplace-Name: Technical Institute for Field Crops (ITGC), Sétif, Algeria$4 Author-Name: Antonio Michele STANCA Author-Workplace-Name: Genomics Research Centre, Fiorenzuola d'Arda, Italy Title: Marker characterization of vernalization and low-temperature tolerance loci in barley genotypes adapted to semi-arid environments Abstract: For the purpose of marker assisted selection (MAS), six Algerian cultivars and landraces developed locally or introduced from external breeding programs for semi-arid environments were marker characterized at VRN-H1, VRN-H2 and FR-H2 loci. As controls the lines Nure and Tremois were used. Markers available in the literature, known to discriminate efficiently the trait-associated alleles between the two cultivars Nure and Tremois, were used. This study revealed that the used Algerian cultivars carry a dominant (spring) allele for VRN-H1, while the HvZCCT marker generated a polymorphic profile for the second vernalization locus VRN-H2. No cultivars possessed the Nure allele conferring tolerance to low-temperatures at the HvCBF4. Three cultivars possessed the Tremois allele at FR-H2, while the remaining three presented different haplotypes. Keywords: Algerian cultivars, growth habit, low-temperature, MAS, VRN-H1, VRN-H2, FR-H2 Journal: Czech Journal of Genetics and Plant Breeding Pages: 157-162 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/16/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/16/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201604-0006.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:16-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Assefa AMELEWORK Author-Workplace-Name: African Center for Crop Improvement, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa Author-Name: Mark LAING Author-Workplace-Name: African Center for Crop Improvement, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa Author-Name: Hussein SHIMELIS Author-Workplace-Name: African Center for Crop Improvement, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa Title: Evaluation of effective gametocides for selective induction of male sterility in sorghum Abstract: Effective gametocides can serve as alternative agents to induce male sterility in sorghum. The objectives of this study were to evaluate the effectiveness of selected male gametocides in sorghum, to determine the optimum concentration of the chemicals and to investigate its possible effect on female sterility. Ethyl 4-fluorooxanilate (E4FO) and ethrel were evaluated on three genotypes of sorghum. The gametocides were sprayed at five concentrations at the booting stage when the panicles were released halfway from the flag leaf. Pollen and spikelet sterility were assessed using light and scanning electron microscopes. Both chemicals were effective in inducing male sterility in sorghum. However, E4FO was the more potent chemical, inducing complete male sterility with high female fertility and relatively less phytotoxicity at lower concentrations, while higher concentrations of ethrel were required to give complete male sterility. E4FO induced on average of 99.5% male sterility at 2 mg/l in all the varieties, while with ethrel 97% male sterility was obtained at 3 ml/l. Therefore we recommend to use E4FO at 2 mg/l and ethrel at 3 ml/l as male sterilizing agents to facilitate controlled crosses in sorghum. Keywords: chemical hybridizing agent, ethrel, ethyl 4-fluorooxanilate, gametocide, pollen sterility, spikelet sterility, Sorghum bicolor Journal: Czech Journal of Genetics and Plant Breeding Pages: 163-170 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/159/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/159/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201604-0007.txt Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:159-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: J. Chrpová Title: Ing. Václav Šíp, CSc. Journal: Czech Journal of Genetics and Plant Breeding Pages: 171 Volume: 52 Issue: 4 Year: 2016 DOI: 10.17221/179/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/179/2016-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:52:y:2016:i:4:id:179-2016-CJGPB