Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: LIST OF REVIEWERS 2016 Journal: Czech Journal of Genetics and Plant Breeding Pages: I Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/9572-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/9572-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:9572-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Teerawat SUWANNUAL Author-Workplace-Name: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Sompong CHANKAEW Author-Workplace-Name: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Tidarat MONKHAM Author-Workplace-Name: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Weerasak SAKSIRIRAT Author-Workplace-Name: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Jirawat SANITCHON Title: Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection Abstract: Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC2F2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research. Keywords: broad spectrum, introgression lines, Magnaporthe oryzae, Oryza sativa, severity index Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-8 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/51/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/51/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201701-0002.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:51-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Morufat BALOGUN Author-Name: Norbert MAROYA Author-Workplace-Name: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Author-Name: Joao AUGUSTO Author-Workplace-Name: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Author-Name: Adeola AJAYI Author-Workplace-Name: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Author-Name: Lava KUMAR Author-Workplace-Name: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Author-Name: Beatrice AIGHEWI Author-Workplace-Name: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Author-Name: Robert ASIEDU Author-Workplace-Name: International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Title: Relative efficiency of positive selection and tissue culture for generating pathogen-free planting materials of yam (Dioscorea spp.) Abstract: Yams are staples in West Africa. They are propagated from tubers in an informal seed system. This encourages a build-up of diseases, and necessitates the rapid development of a formal seed system where certified seeds are functional. Although few reports exist on the use of meristem culture to generate pathogen-free yam, the success rate for the most economically important species in the sub-region, Dioscorea rotundata, for the most prevalent viruses is inadequate. To generate pathogen-free yam planting materials, the relative efficiency of tissue culture and positive selection was compared. Twenty-one asymptomatic yam plants were positively selected from 8187 stands of five landraces. Five of these stands were tested virus-negative by multiplex polymerase chain reaction (PCR) for Yam mosaic virus (YMV), Yam mild mosaic virus (YMMV) and Cucumber mosaic virus (CMV), and by PCR for the genus Badnavirus (BV), giving 0.08% success. Single nodes of the positively selected stands were used to establish in vitro plantlets, which were screened onto bacteriological indexing medium. The same was done for meristem- and node-derived plantlets of the improved variety TDr 95/19158. Incidence of endophytes ranged from 18 to 32% in the nodal plantlets while it was 0% in the meristem-derived plantlets. The effect of meristem culture combined with thermotherapy on the virus infection status was determined using virus-tested, one week old in vitro plantlets of eight improved genotypes. These in vitro plantlets were incubated at 36 ± 0.5°C and 16 h photoperiod for 21 days, after which meristems were excised, regenerated into plantlets and re-tested for viruses. Seventy-three percent of the samples were recovered from YMV but the effect on BV was inconsistent. Positive selection can be used as a palliative in generating quality declared seed but meristem culture combined with thermotherapy is more efficient for generating certified seed tubers of yam. Keywords: asymptomatic selections, endophytes, micropropagation, seed systems, thermotherapy, yam virus diseases Journal: Czech Journal of Genetics and Plant Breeding Pages: 9-16 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/117/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/117/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201701-0003.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:117-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Katarzyna MAKOWSKA Author-Name: Sylwia OLESZCZUK Author-Workplace-Name: Plant Breeding and Acclimatization Institute, National Research Institute (IHAR-PIB) Radzikow, Blonie, Poland Author-Name: Janusz ZIMNY Author-Workplace-Name: Plant Breeding and Acclimatization Institute, National Research Institute (IHAR-PIB) Radzikow, Blonie, Poland Title: The effect of copper on plant regeneration in barley microspore culture Abstract: Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture. Keywords: albinism, androgenesis, doubled haploid, Hordeum vulgare, regenaration efficiency Journal: Czech Journal of Genetics and Plant Breeding Pages: 17-22 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/82/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/82/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201701-0004.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:82-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ning ZHANG Author-Workplace-Name: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China Author-Name: XU Bing-hua Author-Workplace-Name: Huaiyin Institute of Agricultural Sciences of Xuhuai Region, Jiangsu P.R. China Author-Name: BI Yan-fei Author-Workplace-Name: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China Author-Name: Qun-Feng LOU Author-Workplace-Name: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China Author-Name: Jin-Feng CHEN Author-Workplace-Name: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China Author-Name: Chun-Tao QIAN Author-Workplace-Name: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China Author-Name: Yong-Bing ZHANG Author-Workplace-Name: Center of Hami Melon, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, P.R. China$2 Author-Name: YI Hong-ping Author-Workplace-Name: Center of Hami Melon, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, P.R. China$2 Title: Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramiding Abstract: Gummy stem blight (GSB), caused by Didymella bryoniae (Auersw.) Rehm., is a severe disease affecting Cucurbitaceae crops including melons. The resistance of current melon varieties that carry a single Gsb resistance gene is insufficient to protect against the abundant variation of the D. bryoniae isolates. Pyramiding multiple Gsb resistance genes into melon cultivars is an effective way to develop a broad resistance spectrum and to increase the duration of GSB resistance. In this study, two resistance genes (Gsb-4 and Gsb-6) from two resistant accessions, PI482398 and PI420145, were pyramided into one variety using marker-assisted selection (MAS). The donor parent 4598 that contained Gsb-4 and Gsb-6 was hybridized with muskmelon Baipicui to produce BC1F1 and BC2F1. Phenotyping and MAS enabled identification and pyramiding of two Gsb genes in individuals of F1, BC1F1 and BC2F1. Field cultivation showed that individuals carrying both Gsb-4 and Gsb-6 had improved resistance to GSB and improved fruit quality. The results indicated that MAS-based pyramiding is an effective strategy for breeding melon cultivars with increased resistance to GSB. Keywords: Cucumis melo, Didymella bryoniae, disease resistance breeding, fruit quality, marker-assisted selection Journal: Czech Journal of Genetics and Plant Breeding Pages: 23-29 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/84/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/84/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201701-0005.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:84-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zainab Majed ALMUTAIRI Title: Expression profiling of certain MADS-box genes in Arabidopsis thaliana plant treated with silver nanoparticles Abstract: Silver nanoparticles (AgNPs) have been shown to enhance seed germination and plant growth. In this study, we examined the plant response to AgNP dosage using a model plant, Arabidopsis thaliana. Seedlings were treated with different concentrations of AgNPs. Seedling fresh and dry weights were measured for all treatments. The exposure of plants to 1 mg/l AgNPs resulted in a significant increase in seedling fresh and dry weights compared with control plants, although the exposure to lower and higher concentrations resulted in a decrease in fresh and dry weights. Expression profiling for 22 MADS-box genes was carried out using quantitative real-time PCR (qRT-PCR). Among the investigated MADS-box genes, eight genes were upregulated and four genes were downregulated at each of the AgNP concentrations, 0.1 and 1 mg/l. Revealing the effects of AgNPs on the expression of MADS-box genes will increase our knowledge of their role before implementing a large-scale agricultural utilization of AgNPs in the improvement of plant growth and development. Keywords: AgNPs, molecular mechanisms, plant growth, qRT-PCR Journal: Czech Journal of Genetics and Plant Breeding Pages: 30-36 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/130/2015-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/130/2015-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201701-0006.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:130-2015-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jiří HERMUTH Author-Workplace-Name: Department of Plant Genetics, Breeding and Product Quality, Crop Research Institute, Prague, Czech Republic Author-Name: Klára KOSOVÁ Title: Characterization of the first Czech sorghum variety Ruzrok tested in Czech Republic Abstract: Ruzrok, the first sorghum cultivar of Czech provenance, was selected as a multiline cultivar from sorghum collected in the Bílé Karpaty region in Moravia, Czech Republic. In the present paper, basic plant morphological and yield-related characteristics as well as characteristics related to plant resistance to major abiotic and biotic stress factors are described. The major advantages of Ruzrok consists in its earliness, plant height and high growth rate of plants leading to high biomass production. Ruzrok was registered for its high biomass yield; however, its grains can also be used for flour production and food purposes after the technological removal of tannin-rich outer layers of grains. Recommendations for basic cultural practices such as sowing date, fertilization and protection against pests and diseases are given. In conclusion, potential utilization of the novel sorghum variety as both biomass and fodder crop is discussed. Keywords: central Europe, earliness, growth- and yield-related characteristics, minority cereals, yield Journal: Czech Journal of Genetics and Plant Breeding Pages: 37-44 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/134/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/134/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201701-0007.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:134-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: T. Mezlík Title: List of field crop varieties registered in the Czech Republic in 2016 Journal: Czech Journal of Genetics and Plant Breeding Pages: 45-46 Volume: 53 Issue: 1 Year: 2017 DOI: 10.17221/31/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/31/2017-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:1:id:31-2017-CJGPB