Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 53 Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 53 Issue: 4 Year: 2017 DOI: 10.17221/10243-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/10243-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:10243-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mohsen NIAZIAN Author-Workplace-Name: Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Pakdasht, Iran Author-Name: Seyed Ahmad SADAT NOORI Author-Workplace-Name: Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Pakdasht, Iran Author-Name: Petr GALUSZKA Author-Workplace-Name: Department of Molecular Biology, Centre of the Haná Region for Biotechnological and Agriculture Research, Palacky University, Olomouc, Czech Republic Author-Name: Seyed Mohammad Mahdi MORTAZAVIAN Author-Workplace-Name: Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Pakdasht, Iran Title: Tissue culture-based Agrobacterium-mediated and in planta transformation methods Abstract: Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than tissue culture-based transformation methods. Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much quicker than regular tissue culture-based Agrobacterium-mediated gene transformation and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular tissue culture-based Agrobacterium-mediated transformation methods and then recently successful transformations using these methods are presented. Keywords: Agrobacterium, floral dip, floral spray, pollen-tube pathway, sonication, vacuum infiltration Journal: Czech Journal of Genetics and Plant Breeding Pages: 133-143 Volume: 53 Issue: 4 Year: 2017 DOI: 10.17221/177/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/177/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201704-0002.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:177-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: WU Kunlun Author-Workplace-Name: Sichuan Agricultural University, Chengdu, P.R. China Author-Workplace-Name: State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Academy of Agricultural and Forestry Sciences of Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, P.R. China Author-Name: Xiaohua YAO Author-Workplace-Name: State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Academy of Agricultural and Forestry Sciences of Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, P.R. China Author-Name: Youhua YAO Author-Workplace-Name: State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Academy of Agricultural and Forestry Sciences of Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, P.R. China Author-Name: Dezhao CHI Author-Workplace-Name: State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Academy of Agricultural and Forestry Sciences of Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, P.R. China Author-Name: Zongyun FENG Author-Workplace-Name: Sichuan Agricultural University, Chengdu, P.R. China Title: Analysis of the relationship between Wx gene polymorphisms and amylose content in hulless barley Abstract: I2-KI staining was used to phenotype 151 hulless barley plants, which determined that five samples were of the waxy variety, namely 14-Z152, IG107028, Puebla, Hu Zhu Shuang Cao Ren, and APM-HC1905. Using the dual-wavelength method, the average amylose content of the 151 samples was 25.9%, ranging from 4.9 to 38.5%. The average amylose content of the five waxy varieties was 14.3%, ranging from 4.9 to 18.6%. Genomic DNA from 48 samples showing a significantly variable amylose content was used as template and PCR amplified using primer pair P4. Statistical analysis indicated that the PCR product size positively correlated with amylose content. The Wx gene locus was determined to be polymorphic, and was positively correlated with amylose. Based on the electrophoresis results, the 48 samples were divided into 4 types. PCR product types I, II, III, and IV were 457, 481, 489, and 491 bp in length, respectively, with the respective amylose content ranges of 4.9-27%, 29-30%, 31-35%, and 36-38%. Primer P4 can be used as a complementary marker for the selection of different amylose content hulless barley germplasms. Keywords: Hordeum vulgare L. var. nudum, molecular marker, starch property, Waxy gene Journal: Czech Journal of Genetics and Plant Breeding Pages: 144-152 Volume: 53 Issue: 4 Year: 2017 DOI: 10.17221/105/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/105/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201704-0003.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:105-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: LI Shengqiang Author-Name: Ruiyue ZHANG Author-Workplace-Name: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China Author-Name: Jipeng CHEN Author-Workplace-Name: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China Author-Name: Jie ZOU Author-Workplace-Name: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China Author-Name: Tao LIU Author-Workplace-Name: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China Author-Name: Guohua ZHOU Author-Workplace-Name: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China Title: Genetic analysis and fine mapping of the RK4 gene for round kernel in rice (Oryza sativa L.) Abstract: Grain shape of rice is an important trait for both yield and quality. A rice rk4 (round kernel) mutant was obtained from the japonica variety Zhonghua 11 by radiation of 60Co-γ. The grain width of the mutant was increased and the length was decreased. Simultaneously, the 1000-grain weight was slightly reduced, therefore the grain shape of the mutant tended to be small and round. In this study, genetic analysis and gene mapping of the mutant gene were carried out using the F2 and F3 populations derived from the mutant and the indica variety Xianhui 8006. The results suggested that the round kernel was controlled by a single recessive allele (rk4) which was located on chromosome 5. The RK4 gene was further mapped between the molecular markers LSTS5-77 and LSTS5-60 with 0.57 and 0.096 cM, respectively. A BAC clone was found to span the RK4 locus, and the RK4 gene was placed in a 46 kb region that contains six annotated genes according to the available sequence annotation database. This result will help us to isolate the RK4 gene and reveal the molecular mechanism of the round kernel in rice. Keywords: BSA, ESEM, kernel shape, SSR, STS Journal: Czech Journal of Genetics and Plant Breeding Pages: 153-158 Volume: 53 Issue: 4 Year: 2017 DOI: 10.17221/172/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/172/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201704-0004.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:172-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Khushboo SETHI Author-Workplace-Name: Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, Haryana, India Author-Name: Priyanka SIWACH Author-Workplace-Name: Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, Haryana, India Author-Name: Surender Kumar VERMA Author-Workplace-Name: Central Institute of Cotton Research, Regional Station, Sirsa, Haryana Title: Linkage disequilibrium and association mapping of fibre quality traits in elite Asiatic cotton (Gossypium arboreum) germplasm populations Abstract: Cotton productivity has been hindered by the narrow genetic base of cultivated cotton. Linkage disequilibrium-based association mapping has become a powerful molecular tool to dissect and exploit genetic diversity. In the present study, population structure and marker-trait associations for fibre quality traits in genotypes belonging to six races of Gossypium arboreum were assessed. Out of 300 simple sequence repeat (SSR) markers, 100 were found polymorphic, yielding a total of 240 alleles (all polymorphic). Structure analysis revealed allelic admixtures between genotypes. A Q-matrix exhibited mixed ancestry for the majority of genotypes, the race indicum forming a significant percent ancestry for almost all genotypes. At significant threshold values of r2 ≥ 0.05, 7.37% of SSR loci showed significant linkage disequilibrium (LD), while at highly significant threshold of r2 ≥ 0.1, the value was reduced to 5.31%. LD clearly decayed within the genetic distance of 9-10 cM, with r2 ≥ 0.1. Twenty-eight SSR markers were found associated with six fibre quality traits using general linear model and mixed linear model. Keywords: general linear model, genetic distance, genetic diversity, mixed linear model, simple sequence repeat Journal: Czech Journal of Genetics and Plant Breeding Pages: 159-167 Volume: 53 Issue: 4 Year: 2017 DOI: 10.17221/142/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/142/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201704-0005.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:142-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Francesca CARUCCI Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Raffaele GARRAMONE Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Riccardo AVERSANO Author-Name: Domenico CARPUTO Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Title: SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon Abstract: In this study, 12 microsatellite markers (SSR) were evaluated for their applicability to protect from frauds and misuse the Italian PGI product "Common bean from Lamon". SSR analysis generated polymorphic alleles, with an average of 4 alleles per locus and all in the range of molecular weight between 181 and 284 bp. Twenty-nine variety-specific fragments were identified, which might be reasonably adopted for characterization and traceability purposes. Cluster analysis well outlined the relationships between the genotypes studied. Overall, our study underlines the use and usefulness of molecular markers to protect both farmers and consumers from frauds. Keywords: fingerprinting, Protected Geographical Indication (PGI), varietal authentication Journal: Czech Journal of Genetics and Plant Breeding Pages: 168-171 Volume: 53 Issue: 4 Year: 2017 DOI: 10.17221/91/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/91/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201704-0006.txt Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:91-2016-CJGPB