Template-Type: ReDIF-Article 1.0 Author-Name: Shu Ming YANG Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Su Hua ZHANG Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Workplace-Name: Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming, Yunnan, P.R. China Author-Name: Tao YANG Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Li WANG Author-Workplace-Name: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, P.R. China Title: Detection of QTLs for cold tolerance at the booting stage in near-isogenic lines derived from rice landrace Lijiangxintuanheigu Abstract: Chilling damage significantly reduces grain yield in rice, while exploring major quantitative trait loci (QTLs) has the potential to improve rice production. Mapping of QTLs for 5 cold tolerance-related traits at the booting stage was conducted with SSR markers and inclusive composite interval mapping (ICIM) approach, based on 105 near-isogenic lines derived from a backcross between Lijiangxintuanheigu (LTH, cold-tolerant landrace) and Towada (cold-sensitive cultivar). Phenotype values were investigated under five cold-stress environments and analysed by the best linear unbiased prediction (BLUP). Twenty-one QTLs were identified on chromosomes 1, 2, 3, 4, 6, 7, 10 and 11, and the amount of variation (R2) explained by each QTL ranged from 7.71 to 29.66%, with five co-located QTL regions. Eight novel major loci (qSF-2, qSF-6a, qSF-7, qGW-6, qDGWP-4, qDSWPP-4, qDWPP-1 and qDWPP-4b) were detected in several environments and BLUP, and their alleles were contributed by LTH with R2 variance from 12.24 to 29.66%. These favourable QTLs would facilitate elucidation of the genetic mechanism of cold tolerance and provide strategies for breeding high-productive rice. Keywords: Oryza sativa, quantitative trait loci, near-isogenic lines, cold tolerance at the booting stage Journal: Czech Journal of Genetics and Plant Breeding Pages: 93-100 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/98/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/98/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0001.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:98-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Hifzur RAHMAN Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Vijayalakshmi DAKSHINAMURTHI Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Sasikala RAMASAMY Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Sudha MANICKAM Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Ashok Kumar KALIYAPERUMAL Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Suchismita RAHA Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Naresh PANNEERSELVAM Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Valarmathi RAMANATHAN Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Jagadeeshselvam NALLATHAMBI Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Robin SABARIAPPAN Author-Workplace-Name: Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India Author-Name: Muthurajan RAVEENDRAN Title: Introgression of submergence tolerance into CO 43, a popular rice variety of India, through marker-assisted backcross breeding Abstract: Submergence is a major threat in rice growing areas of South and South-East Asia. Identification of a major QTL, Sub1 and advancements in marker-assisted backcrossing strategy enabled breeders to develop submergence tolerant versions of popular rice cultivars. In the present study, a marker-assisted backcross breeding (MABB) approach was used to introgress the Sub1 locus from the tolerant FR13A variety into CO 43, one of the popular rice varieties of southern India. Evaluation of early generation segregating progenies derived from the cross of CO 43 and FR13A revealed the introgression of Sub1, leading to increased tolerance to submergence and better revival ability after it. Genotyping and phenotyping of BC3F3 generation resulted in elite NILs of CO 43 harbouring Sub1 the locus and possessing 94.37% and 95.78% of the recurrent parental CO 43 genome. Selected NILs performed equal as the recurrent parent CO 43 under normal conditions, but were more tolerant to submergence and revived better, afterwards. Under flooding NILs yielded about 25-30% higher than the recurrent parent CO 43. Keywords: flooding tolerance, molecular breeding, rice Journal: Czech Journal of Genetics and Plant Breeding Pages: 101-108 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/149/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/149/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0002.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:149-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Xiaopeng SONG Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Jie FENG Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Zixia CUI Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Chuanliang ZHANG Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Daojie SUN Title: Genome-wide association study for anther length in some elite bread wheat germplasm Abstract: The anther is a crucial organ for the development of the spike in bread wheat (Triticum aestivum L.). Long anthers contain large amounts of pollen grains; thus, they are favourable for cross-pollination and increase resilience against adverse environmental conditions. We conducted a genome-wide association study (GWAS) of anther length in 305 elite wheat lines evaluated during 2013-2015 in two locations and two growing seasons. The mapping panel was genotyped using a high-density Illumina iSelect 90K single nucleotide polymorphism (SNP) array. The GWAS used 18763 SNPs and identified 17 markers associated with anther length in wheat. The loci were mainly distributed across the chromosomes 3A, 3B and 7B. Further studies are required to determine if these are candidate genomic regions of anther length. In addition, anther length had high heritability, and positive correlations between anther length and grain weight per spike were observed. Keywords: bread wheat lines, GWAS, marker-trait association, SNP, spike traits Journal: Czech Journal of Genetics and Plant Breeding Pages: 109-114 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/70/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/70/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0003.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:70-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zhijun TONG Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Bingguang XIAO Author-Name: Xuejun CHEN Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Dunhuang FANG Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Yihan ZHANG Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Changjun HUANG Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: LI Yongping Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Title: Construction of a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on SSR markers and comparative studies Abstract: Genetic linkage maps representing the tobacco genome have been an important tool for breeding programs because of the elucidation of polygenic traits. We constructed a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on an inter-type backcross population of 213 individuals and performed a comparative analysis with other published maps of dark tobacco and flue-cured tobacco. The map consisted of 562 SSR loci distributed on 24 tentative linkage groups and spanned a total length of 1341.18 cM with an average distance of 2.39 cM between adjacent markers. The comparative analysis revealed a Spearman correlation index of 0.93 for marker order conservation with the previously published maps constructed for different tobacco types. Approximately 91% of the SSR markers common to other inter-type maps were located in the same positions as in previous maps. The three maps exhibit good synteny in terms of the shared markers, which suggests that there might be no translocation variations between the genomes of the cigar, dark and flue-cured tobaccos. These results indicate the feasibility of generating a unique genetic map of preferred traits in cigar tobacco and that such mapping may be helpful for breeding programs because plants derived from different inter-type populations can be rapidly scanned using the markers associated with useful cigar traits Keywords: common tobacco, comparative mapping, genetic map, microsatellite marker Journal: Czech Journal of Genetics and Plant Breeding Pages: 115-122 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/121/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/121/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0004.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:121-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mehdi JOUDI Author-Workplace-Name: Meshkinshahr College of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran Author-Name: Wim VAN DEN ENDE Author-Workplace-Name: Lab of Molecular Plant Biology, Institute of Botany and Microbiology, KU Leuven, Leuven-Heverlee, Belgium Title: Genotypic variation in pre- and post-anthesis dry matter remobilization in Iranian wheat cultivars: associations with stem characters and grain yield Abstract: The amount of carbohydrate accumulation and remobilization in the wheat stem can be estimated by monitoring changes in stem dry matter. Eighty-one wheat cultivars were examined in the Moghan region of Iran during the 2010-2011 and 2013-2014 growing seasons. Pre- and post-anthesis dry matter remobilization was quantified in the tested cultivars and their associations with stem characteristics and grain yield were investigated. There was substantial variation in stem length, weight, and specific weight among the tested cultivars. The majority of the cultivars did not show pre-anthesis dry matter remobilization in the stem or internodes. In contrast, most of them used stem dry matter that had been stored after anthesis, suggesting that under the given conditions, current photosynthesis along with post-anthesis dry matter remobilization could meet most of the sink demand. Generally, there were no significant associations between stem length and dry matter remobilization (r = -0.05 to 0.03; P ≥ 0.05). Stem weight (r = 0.42 to 0.65; P ≤ 0.01) and specific stem weight (r = 0.44 to 0.60; P ≤ 0.01) measured at 16 days after anthesis correlated positively and significantly with dry weight loss from the stem. Intriguingly, no clear relationships were found between dry matter remobilization and grain yield (r = -0.13 to 0.04; P ≥ 0.05), suggesting that there are no simple relationships between these traits. The association between dry matter remobilization and grain yield may be different depending on the examined cultivars. Keywords: remobilization, stem specific weight, stem weight, wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 123-134 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/93/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/93/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0005.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:93-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: HE Ping Author-Name: LI Linguang Author-Name: Lailiang CHENG Author-Workplace-Name: Shandong Institute of Pomology, Taian, Shandong, P.R. China Author-Name: Haibo WANG Author-Workplace-Name: Shandong Institute of Pomology, Taian, Shandong, P.R. China Author-Name: Yuansheng CHANG Author-Workplace-Name: Shandong Institute of Pomology, Taian, Shandong, P.R. China Title: Variation in ploidy level and morphological traits in the progeny of the triploid apple variety Jonagold Abstract: Variation with respect to both ploidy level and morphology was characterized for a set of 690 seedlings of the triploid apple variety Jonagold, of which 481 were obtained via in vitro culture of mature embryos, and 209 via conventional germination. Their ploidy level was determined by a combination of flow cytometry and root tip chromosome counting. The assessed morphological traits were leaf length, width and shape, stomatal density, guard cell length and chloroplast width and number. A total of 452 seedlings were aneuploid, 225 diploid, nine triploid and four tetraploid. All four tetraploid seedlings were derived by in vitro culture. When the triploid seedlings were genotyped at the S-locus and at selected microsatellite loci, we found that the Jonagold stigma was compatible with pollen which shared some of the maternal parent S-locus alleles. Keywords: flow cytometry, karyotypic analysis, S-alleles, SSR markers Journal: Czech Journal of Genetics and Plant Breeding Pages: 135-142 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/201/2016-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/201/2016-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0006.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:201-2016-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Anna DEPTA Author-Workplace-Name: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, Pulawy, Poland Author-Name: Karolina KURSA Author-Workplace-Name: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, Pulawy, Poland Author-Name: Teresa DOROSZEWSKA Author-Workplace-Name: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, Pulawy, Poland Author-Name: Dorota LASKOWSKA Author-Workplace-Name: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, Pulawy, Poland Author-Name: Anna TROJAK-GOLUCH Title: Reaction of Nicotiana species and cultivars of tobacco to Tobacco mosaic virus and detection of the N gene that confers hypersensitive resistance Abstract: Tobacco mosaic virus (TMV) brings increasing losses in the cultivation of tobacco. Sixty-two cultivars of tobacco and eleven species of Nicotiana were evaluated for resistance to TMV. Biological tests at two temperature ranges, DAS-ELISA and molecular markers were applied to assess the resistance to TMV. Most cultivars of tobacco showed susceptibility (S) to TMV, two were tolerant (T), while others revealed a hypersensitive response (HR). Hypersensitivity, determined by the N gene, occurred only at a temperature below 22°C. At a temperature above 28°C, all the cultivars showed mosaic discolorations or extensive necrosis. The reaction of the Nicotiana species was dependent on growth conditions. At 22°C, the reactions of sensitivity, tolerance and hypersensitivity to TMV were all observed, whereas above 28°C the species showed systemic necrotic symptoms. N. gossei was an exception because hypersensitivity occurred regardless of the thermal conditions. The resistance of this species was not conditioned by the N gene, which suggests that N. gossei could be an additional genetic resource for tobacco breeding. Keywords: genetic resources, Nicotiana gossei, Nicotiana tabacum, TMV Journal: Czech Journal of Genetics and Plant Breeding Pages: 143-146 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/81/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/81/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201803-0007.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:3:id:81-2017-CJGPB