Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 54 Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/11393-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/11393-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:11393-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Sylwia Elzbieta KELLER-PRZYBYŁKOWICZ Title: Structural and functional apple genomics and its application in breeding Abstract: The present paper summarized some knowledge of modern technologies, applied in apple (Malus domestica) genome studies. New generation sequencing allowed single nucleotide polymorphism (SNP) chip technologies for genotyping, description of functional apple genes, characterization of the evolutionary results in apple genome fragment transition, as well as phylogenetic reconstruction of the genus Malus, being the confirmed progeny of M. sieviersii. Based on these technologies, newly developed putative markers may give the most important biological data such as age, geographical origins, tissue type determination, and external visible characters. The new generation genotyping platforms, representing very high efficiency, are now successfully applied for random apple genome-wide association (GWA) studies as well pedigree-based analysis and marker-assisted selection (MAS). Keywords: genotyping, GWA, high-throughput sequencing, fine mapping, MAS Journal: Czech Journal of Genetics and Plant Breeding Pages: 147-153 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/59/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/59/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0002.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:59-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Dharmalingam RAJA Author-Workplace-Name: Rasi Research and Development Center, Rasi Seeds (P) Ltd., Attur, Tamil Nadu, India Author-Name: Marappan SARVANA KUMAR Author-Workplace-Name: Rasi Research and Development Center, Rasi Seeds (P) Ltd., Attur, Tamil Nadu, India Author-Name: Ponnuswamy RENUKA DEVI Author-Workplace-Name: Department of Biotechnology, Anna University, Coimbatore, Tamil Nadu, India Author-Name: Sankaran LOGANATHAN Author-Workplace-Name: Rasi Research and Development Center, Rasi Seeds (P) Ltd., Attur, Tamil Nadu, India Author-Name: Kamalasekharan RAMYA Author-Workplace-Name: Rasi Research and Development Center, Rasi Seeds (P) Ltd., Attur, Tamil Nadu, India Author-Name: Nallathambi KANNAN Author-Workplace-Name: Rasi Research and Development Center, Rasi Seeds (P) Ltd., Attur, Tamil Nadu, India Author-Name: Vaidyanathan SUBRAMANIAN Author-Workplace-Name: Rasi Research and Development Center, Rasi Seeds (P) Ltd., Attur, Tamil Nadu, India Title: Identification of molecular markers associated with genic male sterility in tetraploid cotton (Gossypium hirsutum L.) through bulk segregant analysis using a cotton SNP 63K array Abstract: Genic male sterility (GMS) is one of the most important economic traits for cotton (Gossypium hirsutum L.) hybrid seed production. The GMS trait conferred by two recessive alleles ms5 and ms6 in homozygous constitution is widely used for cotton hybrid seed production in India. Identification of molecular markers closely linked to the ms5 and ms6 alleles would be useful in effective transferring in a short time male-sterility genes into cultivars or elite lines using marker-assisted backcrossing. Here, we describe a quick method to identify markers for GMS genes using bulk segregant analysis (BSA) in the interspecific (G. hirsutum × G. hirsutum) biparental population. The parents and bulks were genotyped with a cotton single nucleotide polymorphism (SNP) 63K array that contains 63 058 SNP markers including 45 104 intraspecific and 17 954 interspecific SNP markers. Four SNP markers were found to be linked with the Ms5 and Ms6 genes. The markers i23493Gh and i46470Gh linked with the Ms5 gene, and other two markers i08605Gh and i08573Gh linked with the Ms6 gene are located on chromosome 12 and 26, respectively. A simple and cost effective tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR) assay was optimized for screening a large number of breeding samples with the identified SNP markers in a short time. The molecular markers developed in this study will facilitate the marker-assisted selection (MAS) and accelerate the development of new GMS lines to use in cotton hybrid seed production. Keywords: Gossypium hirsutum, hybrid, male-fertility, polymorphism, test cross Journal: Czech Journal of Genetics and Plant Breeding Pages: 154-160 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/25/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/25/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0003.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:25-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mingfang FENG Author-Workplace-Name: College of Life Science and Author-Name: Jing CANG Author-Workplace-Name: College of Life Science and Author-Name: Junhong WANG Author-Workplace-Name: College of Life Science and Author-Name: Jian SUN Author-Workplace-Name: College of Agriculture, Northeast Agricultural University, Harbin, P.R. China Author-Name: YU Jing Author-Workplace-Name: College of Life Science and Author-Name: XU Qinghua Author-Workplace-Name: College of Life Science and Author-Name: Da ZHANG Author-Workplace-Name: College of Life Science and Author-Name: Ning YANG Author-Workplace-Name: College of Life Science and Author-Name: LU Qiuwei Author-Workplace-Name: College of Life Science and Author-Name: LV Yan Author-Workplace-Name: College of Life Science and Title: Regeneration and Agrobacterium-mediated transformation of japonica rice varieties developed for a cold region Abstract: So far, a large number of transformation systems have been established for japonica rice, but only a few have been reported for cold-region varieties. In our study, we established highly efficient tissue culture systems for two cold-region rice cultivars, Dongnong 427 and Longdao 14. Plant growth regulator (PGR) levels were optimized by an orthogonal experimental design. The culture ability, constituted by induction and differentiation rate, served as the detection index of orthogonal experiments. The optimal combinations of PGRs for callus induction and regeneration of Dongnong 427 and Longdao 14 were 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) + 2 mg/l 6-benzyladenine (BA) + 4 mg/l kinetin (KIN) + 0.2 mg/l α-naphthaleneacetic acid (NAA) and 1 mg/l 2,4-D + 4 mg/l 6-BA + 4 mg/l KIN + 0.5 mg/l NAA, respectively. Agrobacterium strain EHA 105 containing the plasmid pCAMBIA1301 was used for transformation. The frequency of transient transformation was expressed as the ratio between the number of calli showing GUS expression and the total number of calli kept for staining. The highest transformation efficiency in Dongnong 427 was obtained when calli were immersed in 0.272 OD600 (optical density determined at 600 nm) for 10 min. While it was best for Longdao 14 calli to be infected with 0.592 OD600 for 20 min. Infected calli of the two varieties were co-cultivated on two pieces of sterile filter paper moistened with 1 ml liquid co-cultivation medium for three days. The expression of the GUS gene was confirmed by PCR analysis of plants of both varieties. Keywords: Agrobacterium, GUS expression, round-grained non-glutinous rice, tissue culture, transformation efficiency Journal: Czech Journal of Genetics and Plant Breeding Pages: 161-167 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/86/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/86/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0004.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:86-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: LI Yan-Man Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Lei ZHU Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Hua-Yu ZHU Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Peng-Yao SONG Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Lu-Qin GUO Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Lu-Ming YANG Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Workplace-Name: Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou, P.R. China Title: Genome-wide analysis of the WRKY family genes and their responses to cold stress in watermelon Abstract: The WRKY transcription factors play important roles in various physiological processes, especially in regulating plant resistance to environmental stresses. Watermelon (Citrullus lanatus) plants suffer from various stressful climate conditions during their growth, especially cold stress. However, little information about the exact role of WRKYs in watermelon responses to cold was available. In this study, a total of 57 candidate ClWRKY genes from watermelon genome were identified and they were distributed unevenly on 11 chromosomes. After excluding five ClWRKY genes with incomplete WRKY domains, phylogenetic analysis showed that the remaining 52 ClWRKY genes could be divided into three groups with 11 members in Group 1, 34 in Group 2, and seven in Group 3. The ClWRKY genes in group 2 could be further classified into five subgroups with three members in 2a, five in 2b, 13 in 2c, six in 2d, and seven in 2e, respectively. The expression profiles of ClWRKY genes in response to cold stress could be classified into four types: four ClWRKY genes had little or no change in transcript levels, seven ClWRKY genes had irregular expression patters, 17 ClWRKY genes were upregulated, and 25 ClWRKY genes were downregulated. The different regulation patterns of ClWRKY genes in response to low-temperature treatment revealed that the WRKY gene family was crucial for cold stress tolerance and there were multiple regulatory pathways involved in cold resistance. Keywords: Citrullus lanatus, cold stress, expression profile, phylogenetic analysis, WRKY transcription factor Journal: Czech Journal of Genetics and Plant Breeding Pages: 168-176 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/72/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/72/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0005.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:72-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ying SUN Author-Workplace-Name: Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China Author-Name: Xin CHEN Author-Name: Xiao-Yan GAN Author-Workplace-Name: Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China Author-Name: Zhu-Yun YAN Author-Workplace-Name: Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China Author-Name: MU Di-Xiu Author-Workplace-Name: Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China Author-Name: Qing-Rong WANG Author-Workplace-Name: Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China Title: Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza Abstract: Copalyl diphosphate synthase 1 (SmCPS1) is the first committed enzyme in tanshinone biosynthesis. The promoter region plays an important role in the transcriptional regulation of genes. Mutations in the promoter region may affect gene expression, resulting in changes in the amount of metabolites. In this study, we investigated the SmCPS1 gene promoter region together with the 388 bp downstream from the translation start site and the content of tanshinones of 12 different genotypes of Salvia miltiorrhiza. The cis-elements of SmCPS1 promoter were predicted and analysed by the Plant Transcriptional Regulatory Map database. We found (1) a different correlation between the polymorphism in the promoter region and the contents of tanshinones; (2) functional polymorphic loci - four tandem repeat variations, three indels and five single nucleotide polymorphisms (SNPs) in five cis-elements, three SNPs in exons and two SNPs in introns; (3) the correlation coefficient was higher when only functional (informative) polymorphic loci were considered. These findings have laid the foundation for further exploring the interspecific variation of S. miltiorrhiza and its relationship with the contents of tanshinones. Keywords: cis-elements, copalyl diphosphate synthase 1, danshen, polymorphism Journal: Czech Journal of Genetics and Plant Breeding Pages: 177-182 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/97/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/97/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0006.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:97-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Pranit Kumar MUKHERJEE Author-Workplace-Name: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India Author-Name: Raju MONDAL Author-Workplace-Name: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India Author-Name: Sourav DUTTA Author-Workplace-Name: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India Author-Name: Kanti MEENA Author-Workplace-Name: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India Author-Name: Madhumita ROY Author-Workplace-Name: Department of Biotechnology, Techno India University, Kolkata, West Bengal, India Author-Name: Asit Baran MANDAL Author-Workplace-Name: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India Title: In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation Abstract: An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO3. 1/2 Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie. Keywords: clonal fidelity, molecular markers, ramie, tissue culture, vegetative propagation Journal: Czech Journal of Genetics and Plant Breeding Pages: 183-189 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/79/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/79/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0007.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:79-2017-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Vasileios GREVENIOTIS Author-Name: Evangelia SIOKI Author-Workplace-Name: Cotton Centre, Hellenic Agricultural Organization - DEMETER, Karditsa, Greece Author-Name: Constantinos G. IPSILANDIS Author-Workplace-Name: Regional Administration of Central Macedonia, Thessaloniki, Greece Title: Estimations of fibre trait stability and type of inheritance in cotton Abstract: Traits affecting fibre quality were evaluated in a multi-location environmental experiment. Four main cotton regions in Greece were selected as different environments. Five commercial cotton cultivars were used for evaluation of 10 fibre quality traits. Each cultivar was sown in 10 different fields in each region. Environmental fluctuations within regions affected each quality trait differently showing a different degree of inheritance. Four traits showed the lowest stability index values indicating quantitative inheritance, further four traits with intermediate values indicated determination by a few genes, while the more stable and thus with qualitative inheritance traits were considered to indicate fibre maturity and uniformity. The mean estimation of stability in multi-location experiments was found the same as in multi-genotype evaluation. Two cultivars (Elsa and Celia) were found to be more stable across the Greek environments and two regions favoured stability for almost all traits. Correlations between regions were high and the same was found between genotypes. Keywords: multi-location, genotype and environment interaction, qualitative inheritance, quantitative inheritance Journal: Czech Journal of Genetics and Plant Breeding Pages: 190-192 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/12/2017-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/12/2017-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201804-0008.txt Handle: RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:12-2017-CJGPB