Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 55 Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/12603-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/12603-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:12603-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Yong Gang Li Author-Workplace-Name: Key Laboratory of Cold Crop Breeding Improvement and Physiological Ecology in Heilongjiang Province, Agricultural College, Northeast Agricultural University, P.R. China Author-Workplace-Name: Department of Plant Pathology, University of Georgia, Tifton, USA Author-Name: Dan Jiang Author-Workplace-Name: Key Laboratory of Cold Crop Breeding Improvement and Physiological Ecology in Heilongjiang Province, Agricultural College, Northeast Agricultural University, P.R. China Author-Name: Lan Kun Xu Author-Workplace-Name: Key Laboratory of Cold Crop Breeding Improvement and Physiological Ecology in Heilongjiang Province, Agricultural College, Northeast Agricultural University, P.R. China Author-Name: Si Qi Zhang Author-Workplace-Name: Key Laboratory of Cold Crop Breeding Improvement and Physiological Ecology in Heilongjiang Province, Agricultural College, Northeast Agricultural University, P.R. China Author-Name: Ping Sheng Ji Author-Workplace-Name: Department of Plant Pathology, University of Georgia, Tifton, USA Author-Name: Hong Yu Pan Author-Workplace-Name: College of Plant Sciences, Jilin University, Changchun, P.R. China Author-Name: Bai Wen Jiang Author-Workplace-Name: Resources and Environment College, Northeast Agricultural University, Harbin, P.R. China Author-Name: Zhong Bao Shen Author-Workplace-Name: Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, P.R. China Title: Evaluation of diversity and resistance of maize varieties to Fusarium spp. causing ear rot in maize under conditions of natural infection Abstract: Fusarium ear rot in maize (Zea mays L.) is a serious disease in all maize-growing areas worldwide. A total of 454 fungal strains were isolated from 69 commercial maize hybrids grown in Harbin, China, and comprised Fusarium subglutinans (34.8%), F. proliferatum (31.3%), F. verticillioides (20%), F. graminearum (9.7%), and F. equiseti (4.2%). Among them, a complex of multiple species, F. subglutinans, F. proliferatum, and F. verticillioides are the dominant fungi causing ear rot. Among 59 commercial maize hybrids, eleven hybrids (18.6%) were found to be highly resistant to Fusarium ear rot. Simple sequence repeat (SSR) analysis using six pairs of primers resulted in 24 reproducible bands and cluster analysis separated the maize hybrids into eight groups. There was little genetic variation associated with disease resistance. No correlation was found between genetic diversity and disease resistance. Keywords: disease resistance, Fusarium ear rot, genetic diversity, Zea mays Journal: Czech Journal of Genetics and Plant Breeding Pages: 131-137 Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/81/2018-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/81/2018-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201904-0002.txt Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:81-2018-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Salem Marzougui Author-Workplace-Name: Pôle Régional de Recherche Développement Agricoles du Nord-Ouest semi-aride à El Kef, Institution de la Recherche et de l'Enseignement Supérieur Agricoles (IRESA), Tunisia Author-Workplace-Name: Field crops Laboratory, INRAT, University of Carthage, Tunisia Author-Name: Mohamed Kharrat Author-Workplace-Name: Field crops Laboratory, INRAT, University of Carthage, Tunisia Author-Name: Mongi ben Younes Author-Workplace-Name: Pôle Régional de Recherche Développement Agricoles du Nord-Ouest semi-aride à El Kef, Institution de la Recherche et de l'Enseignement Supérieur Agricoles (IRESA), Tunisia Title: Marker-trait associations of yield related traits in bread wheat (Triticum aestivum L.) under a semi-arid climate Abstract: Identifying QTLs (quantitative trait loci) that control yield related traits under a stressed environment is very useful for marker-assisted selection (MAS). Marker-trait associations (MTA) for several agro-morphological traits were performed with 130 Tunisian and exotic spring bread wheat (Triticum aestivum L.) accessions under a semi-arid climate in El Kef, Tunisia. Grain yield and other important traits were evaluated. A population structural analysis identified two sub populations. In total, 29 MTAs were detected at -log P ≥ 3 using an MLM (mixed linear model), and only 5 MTAs with -log P ≥ 4. The locus on chromosome 4A was detected to control the heading date accounting for up to 22% of the trait variance. Two other loci located on chromosomes 3B and 7B were found to be stable during the two cropping seasons and have a pleiotropic effect on the heading date, yield, internodes length and grain per spike. These two regions are candidates for further genetic analysis. Keywords: association mapping, candidate genes, yield Journal: Czech Journal of Genetics and Plant Breeding Pages: 138-145 Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/154/2018-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/154/2018-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201904-0003.txt Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:154-2018-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Shu Ming Yang Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Fei Fei Zhang Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Workplace-Name: Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming, Yunnan, P.R. China Author-Workplace-Name: Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming, Yunnan, P.R. China Author-Name: Su Hua Zhang Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Workplace-Name: Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming, Yunnan, P.R. China Author-Workplace-Name: Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming, Yunnan, P.R. China Author-Name: Gui Yong Li Author-Workplace-Name: Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Li Qiong Zeng Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Guan Suo Liu Author-Workplace-Name: Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Xiao Fen Yu Author-Workplace-Name: Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Xue Li Qiu Author-Workplace-Name: Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, P.R. China Title: QTL mapping of physiological traits at the booting stage in rice under low temperature combined with nitrogen fertilization Abstract: Further dissection of physiological molecular mechanisms is indispensable to alleviate rice yield losses resulting from cold injury. By using 105 near-isogenic lines (NILs) derived from a backcross between cv. Lijiangxintuanheigu (LTH) and cv. Towada, we detected quantitative trait loci (QTLs) for physiological traits of the rice flag leaf, based on polymorphic simple sequence repeat (SSR) markers, inclusive composite interval mapping (ICIM), mixed composite interval mapping (MCIM) approaches and phenotypic value subjected to combine with cold-water stress and three nitrogen application rates. By using ICIM, a total of 34 QTLs with additive effects (A-QTLs) were identified on chromosomes 1, 3, 4, 5, 6, 7 and 10, and the phenotypic variation (R2) explained by each QTL ranged from 8.46 to 29.14%. By using MCIM, 20 A-QTLs and 14 pairs of QTLs with epistatic × environment interaction effects (Epistatic QTLs) were detected, the contribution of environment interaction (H2AE) was 0.87 to 7.36%, while the contribution rates of E-QTL were from 0.97 to 3.58%. Fourteen A-QTLs were detected by ICIM and MCIM, which may serve as a basis for fine-mapping and candidate gene studies, and providing strategies for the development of cold-tolerant rice cultivars and nitrogen application to alleviate chilling stress. Keywords: antioxidant enzymes, cold stress, japonica rice (Oryza sativa L.), nitrogen, QTLs Journal: Czech Journal of Genetics and Plant Breeding Pages: 146-155 Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/67/2018-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/67/2018-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201904-0004.txt Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:67-2018-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jia-Hao Su Author-Name: Tian-Hui Bai Author-Name: Fei Wang Author-Workplace-Name: State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou , P.R. China Author-Name: Ai-Ke Bao Title: Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency Abstract: Alfalfa planting is threatened by limited arable land, salinization, water shortage, and soil nutrient deprivation. To deal with this challenge, we previously introduced the Arabidopsis type I H+-pyrophosphatase gene AVP1 into alfalfa and found that transgenic lines exhibited enhanced tolerance to short-term salinity or drought. In this study, the growth performances of two transgenic lines were further investigated under long-term salinity or drought conditions, as well as under phosphate deficiency (low-Pi). Compared with wild-type (WT) plants, the transgenic alfalfa showed better growth performance with taller plants and more biomass accumulation after being treated with either long-term salinity, long-term drought, or low-Pi. Most importantly, the overexpression of AVP1 significantly increased the root dry weight and the root/shoot ratio of transgenic alfalfa. A more robust root system facilitates the transgenic alfalfa to absorb nutrients, and in turn promotes the growth of the plants. Whether being treated with low-Pi or not, transgenic plants showed higher total phosphorus concentrations by 16.5-35.5% than WT plants. This study laid a foundation for breeding alfalfa cultivars adapted to saline, arid and nutrient-deprived marginal land. Keywords: AVP1 gene, growth performance, phosphorus nutrition, stress tolerance, transgenic alfalfa Journal: Czech Journal of Genetics and Plant Breeding Pages: 156-161 Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/134/2018-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/134/2018-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201904-0005.txt Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:134-2018-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Miroslav Klíma Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Eva Jozová Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Irena Jelínková Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Vratislav Kučera Author-Workplace-Name: Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Shengwu Hu Author-Workplace-Name: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Vladislav Čurn Author-Workplace-Name: Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Title: Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos Abstract: Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested. Keywords: cytoplasmic male sterility, doubled haploids, marker-assisted selection, microspore culture, orf224-1, Rf-gene Journal: Czech Journal of Genetics and Plant Breeding Pages: 162-165 Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/94/2018-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/94/2018-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201904-0006.txt Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:94-2018-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ping He Author-Name: Linguang Li Author-Name: Haibo Wang Author-Workplace-Name: Shandong Institute of Pomology, Taian, Shandong, P.R. China Author-Name: Yuansheng Chang Author-Workplace-Name: Shandong Institute of Pomology, Taian, Shandong, P.R. China Title: An RNA-Seq analysis of the peach transcriptome with a focus on genes associated with skin colour Abstract: Red pigmentation of the skin is an important consumer trait in peach (Prunus persica). The pigment consists largely of anthocyanin. Here, a transcriptomic contrast, based on RNA-Seq technology, was drawn between a white-skinned (Feitao) and a red-skinned (Qiuxue) peach cultivar. The analysis identified 2407 genes as differentially transcribed in the fruit skin of the two cultivars. Among these were a number of genes known to contribute to anthocyanin synthesis. A quantitative real-time PCR assay was used to validate the RNA-Seq-based estimates of transcript abundance for 14 differentially transcribed genes. Anthocyanin synthesis was observed in the skin of Qiuxue fruit during the late ripening stage, matching the high transcript abundance of the gene encoding UDP glucose: flavonoid 3-O-glucosyltransferase, the final step in the synthesis of anthocyanin. Keywords: anthocyanin, pericarp colour, Prunus persica, sequencing Journal: Czech Journal of Genetics and Plant Breeding Pages: 166-169 Volume: 55 Issue: 4 Year: 2019 DOI: 10.17221/90/2018-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/90/2018-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-201904-0007.txt Handle: RePEc:caa:jnlcjg:v:55:y:2019:i:4:id:90-2018-CJGPB