Template-Type: ReDIF-Article 1.0 Author-Name: Shui Qin Li Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, P.R. China Author-Name: Hua Ping Tang Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, P.R. China Author-Name: Han Zhang Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, P.R. China Author-Name: Yang Mu Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, P.R. China Author-Name: Xiu Jin Lan Author-Name: Jian Ma Title: A 1BL/1RS translocation contributing to kernel length increase in three wheat recombinant inbred line populations Abstract: The 1BL/1RS wheat-rye translocation has been widely utilized in wheat genetic improvement and breeding programs. Our understanding on the effects of the 1BL/1RS translocation on wheat kernel size (e.g. length and width) is limited despite of numerous studies reporting about the effects on kernel weight. Here, we identified a wheat 1BL/1RS translocation line 88-1643 with higher kernel length (KL) using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and molecular markers. To detect the possible role of the 1BL/1RS translocation in KL, kernel width (KW), and thousand-kernel weight (TKW), three recombinant inbred line (RIL) populations were constructed by crossing 88-1643 and three other wheat lines. As expected, the results showed that the values of KL in lines carrying 1RS were significantly higher than those carrying 1BS in three RIL populations at multiple environments, indicating that a major and stably expressed allele or gene responsible for increasing KL is most likely located on 1RS from 88-1643. Additionally, in one RIL population, the increased KL contributed significantly to the increase in TKW. Collectively, the 1BL/1RS translocation reported here is of interest to reveal molecular mechanism of the gene controlling KL and will be useful for improving wheat yield. Keywords: correlation, multiple environments, validation populations, wheat yield Journal: Czech Journal of Genetics and Plant Breeding Pages: 43-51 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/79/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/79/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202002-0001.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:79-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Cong Cong Yang Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Jian Ma Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Cong Li Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Min Sun Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Ya Ya Zou Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Ting Li Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Yang Mu Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Hua Ping Tang Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Author-Name: Xiu Jin Lan Author-Workplace-Name: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China Author-Workplace-Name: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, P.R. China Title: The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.) Abstract: Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into the genetic map. All of these three markers were validated in three populations with different genetic backgrounds. 0C98-411 is the most closely linked marker that could trace QTgw.sau-2D in molecular marker assisted breeding. Keywords: high-resolution melting marker, marker development, QTgw.sau-2D, sequence-characterised amplified region marker Journal: Czech Journal of Genetics and Plant Breeding Pages: 52-61 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/35/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/35/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202002-0002.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:35-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Shahril Ab Razak Author-Workplace-Name: Agri-Omic & Bioinformatic Program, Biotechnology and Nanotechnology Research Centre, MARDI Headquarter, Serdang, Selangor, Malaysia Author-Name: Nor Helwa Ezzah Nor Azman Author-Workplace-Name: Agri-Omic & Bioinformatic Program, Biotechnology and Nanotechnology Research Centre, MARDI Headquarter, Serdang, Selangor, Malaysia Author-Name: Rahiniza Kamaruzaman Author-Workplace-Name: Rice Breeding Program, Rice Research Centre, MARDI Seberang Perai, Kepala Batas, Pulau Pinang, Malaysia Author-Name: Shamsul Amri Saidon Author-Workplace-Name: Rice Breeding Program, Rice Research Centre, MARDI Seberang Perai, Kepala Batas, Pulau Pinang, Malaysia Author-Name: Muhammad Fairuz Mohd Yusof Author-Workplace-Name: Agri-Omic & Bioinformatic Program, Biotechnology and Nanotechnology Research Centre, MARDI Headquarter, Serdang, Selangor, Malaysia Author-Name: Siti Norhayati Ismail Author-Workplace-Name: Agri-Omic & Bioinformatic Program, Biotechnology and Nanotechnology Research Centre, MARDI Headquarter, Serdang, Selangor, Malaysia Author-Name: Mohd Azwan Jaafar Author-Workplace-Name: Agri-Omic & Bioinformatic Program, Biotechnology and Nanotechnology Research Centre, MARDI Headquarter, Serdang, Selangor, Malaysia Author-Name: Norzihan Abdullah Author-Workplace-Name: Agri-Omic & Bioinformatic Program, Biotechnology and Nanotechnology Research Centre, MARDI Headquarter, Serdang, Selangor, Malaysia Title: Genetic diversity of released Malaysian rice varieties based on single nucleotide polymorphism markers Abstract: Understanding genetic diversity is a main key for crop improvement and genetic resource management. In this study, we aim to evaluate the genetic diversity of the released Malaysian rice varieties using single nucleotide polymorphism (SNP) markers. A total of 46 released Malaysian rice varieties were genotyped using 1536 SNP markers to evaluate their diversity. Out of 1536 SNPs, only 932 SNPs (60.7%) represented high quality alleles, whereas the remainder either failed to amplify or had low call rates across the samples. Analysis of the 932 SNPs revealed that a total of 16 SNPs were monomorphic. The analysis of the SNPs per chromosome revealed that the average of the polymorphic information content (PIC) value ranged from 0.173 for chromosome 12 to 0.259 for chromosome 11, with an average of 0.213 per locus. The genetic analysis of the 46 released Malaysian rice varieties using an unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed the presence of two major groups. The analysis was supported by the findings from the STRUCTURE analysis which indicated the ∆K value to be at the highest peak at K = 2, followed by K = 4. The pairwise genetic distance of the shared alleles showed that the value ranged from 0.000 (MR159-MR167) to 0.723 (MRIA-Setanjung), which suggested that MR159 and MR167 were identical, and that the highest dissimilarity was detected between MRIA 1 and Setanjung. The results of the study will be very useful for the variety identification, the proper management and conservation of the genetic resources, and the exploitation and utilisation in future breeding programmes. Keywords: DNA marker, genetic relationship, Oryza sativa Journal: Czech Journal of Genetics and Plant Breeding Pages: 62-70 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/58/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/58/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202002-0003.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:58-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Kateřina Baránková Author-Workplace-Name: Mendeleum, Faculty of Horticulture, Mendel University Brno, Lednice, Czech Republic Author-Name: Radek Sotolář Author-Workplace-Name: Department of Viticulture and Enology, Faculty of Horticulture, Mendel University Brno, Lednice, Czech Republic Author-Name: Miroslav Baránek Author-Workplace-Name: Mendeleum, Faculty of Horticulture, Mendel University Brno, Lednice, Czech Republic Title: Identification of rare traditional grapevine cultivars using SSR markers and their geographical location within the Czech Republic Abstract: The designation of traditional varieties of grapevine is usually based on verbal information or very dated records. Old rare cultivars found in the Czech Republic were identified by Simple Sequence Repeat (SSR) analysis, a generally accepted method for cultivar identification. These cultivars are primarily maintained in a national collection of genetic resources. Finally a total of 102 candidate genotypes was selected where 49 traditional varieties were identified on the base of nine SSR loci compared with the European Vitis Database. Thirty-six items were registered under the correct designation. The remaining genotypes included four clones, and two genotypes could be described as synonyms. Seven genotypes were found to be incorrectly marked. For three of them, the correct name was found in the database under their SSR profile and four items were considered to be unique as no identical profile was found. Keywords: DNA markers, genetic resources, Vitis Journal: Czech Journal of Genetics and Plant Breeding Pages: 71-78 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/61/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/61/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202002-0004.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:61-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: John E. Erpelding Author-Workplace-Name: Crop Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, USA Title: Genetic characterisation of the petal spot phenotype for Gossypium arboreum accession PI 408798 Abstract: The occurrence of floral petal spots is common among flowering plants and plays a major role in attracting pollinators. Cotton genotypes having large red petal spots are frequent in the United States Department of Agriculture, National Plant Germplasm System Gossypium arboreum (L.) collection. One accession, PI 408798, showed a unique faint red petal spot phenotype. To genetically characterise this trait, a F2 population of 226 plants was developed by crossing PI 408798 with G. arboreum accession PI 529714 that lacked pigmented petal spots. From the population, 161 plants showed the presence of faint red petal spots; whereas, 65 plants lacked pigmented petal spots. These data supported the single dominant gene model for the presence of floral petal spots. Accession PI 408798 will provide an important genetic resource to characterise the genes involved in the pathway controlling floral pigmentation. Keywords: anthocyanin, Asiatic cotton, floral pigmentation, germplasm Journal: Czech Journal of Genetics and Plant Breeding Pages: 79-83 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/88/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/88/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202002-0005.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:88-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Alena Hanzalová Author-Name: Hana Reimarová Title: Ing. Pavel Bartoš, DrSc. - 90th birthday Journal: Czech Journal of Genetics and Plant Breeding Pages: 84 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/21/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/21/2020-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:21-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tomáš Mezlík Title: List of field crop varieties registered in the Czech Republic in 2019 Journal: Czech Journal of Genetics and Plant Breeding Pages: 85-86 Volume: 56 Issue: 2 Year: 2020 DOI: 10.17221/19/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/19/2020-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:19-2020-CJGPB