Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 56 (2020) - Authors Index Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/12991-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/12991-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:12991-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Manfred Schönleben Author-Workplace-Name: Department of Statistics and Operation Analysis, Faculty of Business and Economics, Mendel University, Brno, Czech Republic Author-Name: Joachim Mentschel Author-Workplace-Name: Department of Global Business Development, Sano - The Animal Nutritionists, Grafenwald, Loiching, Germany Author-Name: Luboš Střelec Author-Workplace-Name: Department of Statistics and Operation Analysis, Faculty of Business and Economics, Mendel University, Brno, Czech Republic Title: Potentials to breed for improved fibre digestibility in temperate Czech maize (Zea mays L.) germplasm Abstract: Cell wall digestibility is an important quality trait of modern silage maize cultivars. The symbiotic relationship between microbes and ruminant livestock enables the efficient upcycling of otherwise for human consumption unsuitable rumen digestible fibre or cell wall components into highly nutritious milk and meat. Before entering the Czech National List of Plant Varieties, new silage maize germplasm is extensively tested for different cell wall digestibility parameters. Recently published, the undigestible neutral detergent fibre (uNDF) cell wall digestibility approach promises even greater practical relevance. The aim of our study was, therefore, to assess the potential of the uNDF method, compared with current standard procedures, using a vast set of official Czech plant variety trial evaluations and Czech silage analyses from the 2018 cropping season. The uNDF method yielded a twice as high phenotypic standard deviation, compared with the current standard approaches. This is good news for plant breeders, official variety testing organisations, and farm professionals alike, enabeling faster variety improvement and simpler variety selection. On the other hand, due to the low differentiation potential, we discourage the use of the absolute lignin content when selecting for digestible silage maize varieties. Since between the digestibility traits enzymatic soluble organic substance (ELOS) and cellulase digestibility (DCS), a Pearson correlation close to one was observed, the substitution of one of these analytics by the uNDF method, may render valuable additional information in a highly economical manner. Keywords: cell wall digestibility, lignin, phenotypic variation, uNDF Journal: Czech Journal of Genetics and Plant Breeding Pages: 133-139 Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/11/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/11/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202004-0002.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:11-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Giedrius Petrauskas Author-Name: Eglė Norkevičienė Author-Workplace-Name: Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Akademija, Lithuania Author-Name: Vaclovas Stukonis Author-Workplace-Name: Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Akademija, Lithuania Author-Name: Vilma Kemešytė Author-Workplace-Name: Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Akademija, Lithuania Title: Phenotypic traits for wild red clover seed yield under drought conditions Abstract: Changes in the gene pool and homogeneity of red clover cultivars occur over time. Therefore, it is necessary to constantly renew the breeding material and foundation seed. Moreover, the market also prescribes a new demand. Based on the Natura 2000 habitats profile, we collected seeds from 39 locations across Lithuania in 2016. The phenotypic traits that affect the seed yield of red clover were analysed in 2018. The homogeneity of the populations with reference to the seed yield and 1 000 seed weight (TSW) were determined as well. Also, the possibility of obtaining two seed yields per season under drought conditions from wild genotypes of red clover was analysed. We found that the final seed yield mostly depends on the seed number per flower head (SN/FH), which strongly correlated with 1st component of PCA during first (r = 0.91) and second (0.92) harvest. Meanwhile, the cluster analysis showed that the typical wild red clover has a lower seed weight than the cultivars and could be clustered on the basis of seed homogeneity. Finally, based on the seed phenotype and harvest components, there were five prospective accessions (2177, 2871, 2876, 2898 and 2899) for a new cultivar prototype. Keywords: autochthonous clover, G × E interaction, yield determination Journal: Czech Journal of Genetics and Plant Breeding Pages: 140-149 Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/111/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/111/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202004-0003.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:111-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Osama Zuhair Kanbar Author-Workplace-Name: Doctoral School of Plant Science, Szent Istvan University, Gödöllő, Hungary Author-Workplace-Name: Departemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, Hungary Author-Name: Csaba Lantos Author-Workplace-Name: Departemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, Hungary Author-Name: Paul Karumba Chege Author-Workplace-Name: Doctoral School of Plant Science, Szent Istvan University, Gödöllő, Hungary Author-Workplace-Name: Departemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, Hungary Author-Name: Erzsebet Kiss Author-Workplace-Name: Genetics, Microbiology and Biotechnology Institute, Szent Istvan University, Gödöllő, Hungary Author-Name: Janos Pauk Author-Workplace-Name: Departemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, Hungary Title: Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture Abstract: We investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25-87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application. Keywords: breeding, haploid, Triticum aestivum L., wheat Journal: Czech Journal of Genetics and Plant Breeding Pages: 150-158 Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/113/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/113/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202004-0004.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:113-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Josef Patzak Author-Name: Alena Henychová Author-Workplace-Name: Hop Research Institute, Co., Ltd., Žatec, Czech Republic Author-Name: Petr Svoboda Author-Workplace-Name: Hop Research Institute, Co., Ltd., Žatec, Czech Republic Author-Name: Ivana Malířová Author-Workplace-Name: Hop Research Institute, Co., Ltd., Žatec, Czech Republic Title: Assessment of epigenetic methylation changes in hop (Humulus lupulus) plants obtained by meristem culture Abstract: In vitro meristem cultures have been used for the production of hop (Humulus lupulus L.) virus-free rootstocks worldwide, because multipropagation is considered to preserve the genetic stability of the produced plantlet. Nevertheless, in vitro tissue cultures can cause genetic and epigenetic changes. Therefore, we studied the genetic and epigenetic variability of Saaz Osvald's clones, Sládek and Premiant cultivars on the DNA methylation level by methylation-sensitive amplification polymorphism (MSAP). In vitro propagated plants, acclimatised glasshouse rootstocks as well as derived mericlones and control plants under field conditions were used for the analyses. A total of 346 clearly and highly reproducible amplified products were detected in the MSAP analyses within the studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. The demethylation events were comparable to the de novo methylation events. Most demethylation changes were found in the in vitro plants, but only a few of them were found in the derived mericlones under field conditions. In contrast, the de novo methylation changes persisted in the acclimatised plants under glasshouse or field conditions. A hierarchical cluster analysis was used for the evaluation of the molecular genetic variability within the individual samples. The dendrogram showed that the individual samples of the same variety, more or less, clustered together. Because the methylation status varied during the virus-free rootstock production process, we suppose that de/methylation process is a natural tool of epigenetics and evolution in vegetatively propagated plants. Keywords: demethylation and de novo methylation changes, epigenetic variability, hierarchical cluster analysis, mericlones, meristem in vitro tissue cultures, methylation-sensitive amplification polymorphism (MSAP) Journal: Czech Journal of Genetics and Plant Breeding Pages: 159-164 Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/27/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/27/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202004-0005.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:27-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lefkothea Karapetsi Author-Workplace-Name: Laboratory of Molecular Biology of Plants, School of Agricultural Sciences, University of Thessaly, Thessaly, Greece Author-Name: Irini Nianiou-Obeidat Author-Workplace-Name: Laboratory of Genetics and Plant Breeding, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece Author-Name: Antonios Zambounis Author-Workplace-Name: Department of Deciduous Fruit Trees, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization DEMETER, Naoussa, Greece Author-Name: Maslin Osathanunkul Author-Workplace-Name: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand Author-Workplace-Name: Center of Excellence in Bioresources for Agriculture, Industry and Medicine Chiang Mai University, Chiang Mai, Thailand Author-Name: Panagiotis Madesis Author-Workplace-Name: Laboratory of Molecular Biology of Plants, School of Agricultural Sciences, University of Thessaly, Thessaly, Greece Author-Workplace-Name: Institute of Applied Biosciences (INAB), Centre for Research and Technology - Hellas (CERTH), Thessaloniki, Greece Title: Molecular screening of domestic apple cultivars for scab resistance genes in Greece Abstract: Apple scab caused by Venturia inaequalis has the most destructive effects among other phytopathogens in apple crops all over the world. The integration of resistance genes from local and domestic cultivars is a prerequisite for the efficient control of this disease and is a main target in efficient breeding approaches. Across Greece, many domestic apple cultivars are reported without deep knowledge about the presence and diversity of scab resistance genes. In this study, the presence of five resistance genes (Rvi2, Rvi4, Rvi6, Rvi8 and Rvi11) was evaluated across twenty local and domestic apple genotypes, employing twelve molecular markers closely linked to known apple scab resistance loci. Significant differences and polymorphisms among the tested cultivars were detected suggesting that some of them carry a sufficient number of resistance genes. This observed genetic diversity could be exploited in ongoing breeding approaches as a natural source of polygenic resistance against apple scab. Keywords: apple scab, disease resistance, marker-assisted selection, Venturia inaequalis Journal: Czech Journal of Genetics and Plant Breeding Pages: 165-169 Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/119/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/119/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202004-0006.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:119-2019-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jiuzhe Sun Author-Workplace-Name: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China Author-Name: Junting Wang Author-Workplace-Name: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China Author-Name: Dongying Su Author-Workplace-Name: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China Author-Name: Jinchu Yang Author-Workplace-Name: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China Author-Name: Erbin Wang Author-Workplace-Name: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China Author-Name: Shixi Wu Author-Workplace-Name: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China Author-Name: Meng Li Author-Workplace-Name: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China Author-Name: Lin Ma Author-Workplace-Name: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China Title: Discrimination of tobacco cultivars using SCAR and RAPD markers Abstract: Tobacco genetic purity is crucial to maintain the quality of cigarette products in the tobacco industry. To reduce the difficulties in the discrimination of large number of tobacco cultivars in production practice, we developed a two-step identification strategy by using SCAR and RAPD markers. A total of 53 tobacco cultivars were examined in the study. Initially, all the selected cultivars were divided into four groups, each group consisted of seven to seventeen tobacco cultivars based on difference in phenotypes identified by the SCAR markers S4 and S8. Later, in each group, each tobacco cultivar was identified using RAPD fingerprinting by using one to four polymorphic primers, which were selected from 200 random primers. The results showed that all 53 tobacco cultivars could be effectively distinguished by using only two SCAR and seven RAPD markers. The two-step fingerprinting strategy could be used as a convenient and cost-effective tool to discriminate large numbers of tobacco cultivars for production planning in the tobacco industry. Keywords: cultivar, fingerprinting, identification, RAPD, SCAR, Nicotiana tabacum Journal: Czech Journal of Genetics and Plant Breeding Pages: 170-173 Volume: 56 Issue: 4 Year: 2020 DOI: 10.17221/120/2019-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/120/2019-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202004-0007.txt Handle: RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:120-2019-CJGPB