Template-Type: ReDIF-Article 1.0 Author-Name: Xiaocui Yan Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Huimin Zheng Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Peipei Zhang Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Gebrewahid Takele Weldu Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Workplace-Name: College of Agriculture, Aksum University, Shire-Indaslassie, Tigray, Ethiopia Author-Name: Zaifeng Li Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Daqun Liu Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Title: QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL population Abstract: Wheat stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), is one of the most important diseases of bread wheat worldwide. Breeding resistant wheat cultivars is the most economical, effective and environmentally friendly way for controlling wheat stripe rust in China. The Romanian wheat line Fundulea 900 showed good resistance to wheat stripe rust at the adult stage. The present study aimed to map the quantitative trait loci (QTLs) for stripe rust resistance in 176 F2:6 recombinant inbred lines (RIL) derived from the cross of Fundulea 900 × Thatcher. The RIL population was phenotyped for stripe rust (YR) severity at Mianyang in the Sichuan province and Baoding in the Hebei province in the 2016/2017 and 2017/2018 cropping seasons. SSR markers combined with a preferred screened group (PSG) analysis were used to identify the QTLs for stripe rust in the population. Three QTLs for stripe rust resistance were mapped on chromosomes 1AL, 7BL and 7DS, respectively. All three QTLs originated from Fundulea 900 and were detected in all the environments. The QTL on 7DS was provided by the known resistance gene Yr18/Lr34. The two QTLs on chromosomes 1AL and 7BL were explained by 9.2 to 21.5% and 5.1 to 10.1% of the phenotypic variance, respectively and might be new QTLs. The QTLs identified in the study and their closely linked markers can be used for marker-assisted selection (MAS) in wheat breeding programmes. Keywords: APR (adult-plant resistance), molecular mapping, Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), QTLs, SNP array, SSR marker Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-8 Volume: 57 Issue: 1 Year: 2021 DOI: 10.17221/71/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/71/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202101-0001.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:1:id:71-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Haifei Zhao Author-Workplace-Name: Agricultural College of Guizhou University, Guizhou University, Guiyang, P.R. China Author-Name: Wei Yan Author-Workplace-Name: Agricultural College of Guizhou University, Guizhou University, Guiyang, P.R. China Author-Name: Kunjiang Yu Author-Workplace-Name: Agricultural College of Guizhou University, Guizhou University, Guiyang, P.R. China Author-Name: Tianya Wang Author-Workplace-Name: Agricultural College of Guizhou University, Guizhou University, Guiyang, P.R. China Author-Name: Aimal Nawaz Khattak Author-Workplace-Name: Agricultural College of Guizhou University, Guizhou University, Guiyang, P.R. China Author-Name: Entang Tian Title: QTL identification for nine seed-related traits in Brassica juncea using a multiparent advanced generation intercross (MAGIC) population Abstract: Agronomic traits are usually determined by multiple quantitative trait loci (QTLs) that can have pleiotropic effects. A multiparent advanced generation intercross (MAGIC) population is well suited for genetically analysing the effects of multiple QTLs on the traits of interest because it contains more QTL alleles than a biparental population and can overcome the problem of confounding the population structure of the natural germplasm population. We previously developed the B. juncea MAGIC population, derived from eight B. juncea lines with great diversity in agronomic and quality traits. In this study, we show that the B. juncea MAGIC population is also effective for the evaluation of multiple QTLs for complex agronomic traits in B. juncea. A total of twenty-two QTLs for nine seed-related traits were identified, including one QTL for each oil content, seed number per silique and thousand-seed weight; two QTLs for each acid detergent lignin and neutral detergent fibre; three QTLs for each acid detergent fibre and protein content; four QTLs for the seed maturity time; and five QTLs for the white index. Some of these QTLs overlapped. These results should be helpful for further fine mapping, gene cloning, plant breeding and marker-assisted selection (MAS) in B. juncea. Keywords: Brassica juncea, MAGIC population, plant breeding, QTL mapping, seed-related traits Journal: Czech Journal of Genetics and Plant Breeding Pages: 9-18 Volume: 57 Issue: 1 Year: 2021 DOI: 10.17221/73/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/73/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202101-0002.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:1:id:73-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Sandeep Kaur Dhaliwal Author-Workplace-Name: Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Satwinder Kaur Dhillon Author-Workplace-Name: Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: B.S. Gill Author-Workplace-Name: Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Asmita Sirari Author-Workplace-Name: Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Anita Rani Author-Workplace-Name: ICAR - Indian Institute of Soybean Research, Indore, Madhya Pradesh, India Author-Name: Roopan Dhillon Author-Workplace-Name: Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India Title: Combining the null Kunitz trypsin inhibitor and yellow mosaic disease resistance in soybean (Glycine max (L.) Merrill) Abstract: For the wide adoption of soybean varieties, it is desirable, that they are Kunitz trypsin inhibitor (Kti) free and resistant to yellow mosaic disease (YMD). The soybean variety SL525 with YMD resistance was crossed with the YMD susceptible variety NRC101 with a null kti allele. The F5 progeny derived from the cross was screened with two simple sequence repeat (SSR) markers (satt409 and satt322) linked with the null kti allele and the YMD resistance, respectively, and one null kti allele-specific marker. The presence of both desirable traits was further confirmed with the phenotypic data which showed good correlation with the genotypic data. The yield potential of fourteen such identified genotypes having both desirable traits was either at par or superior to SL525, hence, represent improved versions of SL 525. Keywords: marker assisted selection, null kti allele, SSR markers, YMD Journal: Czech Journal of Genetics and Plant Breeding Pages: 19-25 Volume: 57 Issue: 1 Year: 2021 DOI: 10.17221/47/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/47/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202101-0003.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:1:id:47-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Youngmin Park Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Author-Name: Hyangju Kang Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Author-Name: Kyungmin Min Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Author-Name: Nam Hyung Kim Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Author-Name: Minhee Park Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Author-Name: In-Ohk Ouh Author-Workplace-Name: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea Author-Name: Ha-Hyun Kim Author-Workplace-Name: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea Author-Name: Jae-Young Song Author-Workplace-Name: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea Author-Name: Dong-Kun Yang Author-Workplace-Name: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea Author-Name: Eun-Ju Sohn Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Author-Name: Sangmin Lee Author-Workplace-Name: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea Title: Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen in mice Abstract: Rabies remains an infectious disease among humans and animals, and requires the development of an effective vaccine essential to prevent rabies. Advances in molecular biology and biotechnology have led to the development and improvement of many rabies vaccines. Before the third-generation of the vaccine, rabies vaccines were based on the virus itself. Thus, even if effective, these vaccines may not be completely safe, resulting in a strong demand for the development of effective subunit vaccines that do not raise concerns about virus replication and infection in the host. This study investigated the ability of the glycoprotein of the rabies virus to be expressed in tobacco plants (Nicotiana benthamiana) and to induce an immune response in mice. Using a transient transfection, a soluble glycoprotein was successfully expressed in N. benthamiana. Fusing of five histidine residues at the C-terminus enabled the glycoprotein to be easily purified by affinity chromatography. The glycoprotein expressed in the plants was found to be N-glycosylated post-translationally, and the mice immunised with this glycoprotein generated neutralising antibodies against the rabies virus. These results suggest that a glycoprotein produced in the endoplasmic reticulum of N. benthamiana is bioactive, and might be used to generate a subunit vaccine against the rabies virus. Keywords: glycoprotein, molecular pharming, plant-made pharmaceuticals, subunit vaccine, rabies Journal: Czech Journal of Genetics and Plant Breeding Pages: 26-35 Volume: 57 Issue: 1 Year: 2021 DOI: 10.17221/25/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/25/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202101-0004.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:1:id:25-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ya Li Author-Name: Qinhan Yu Author-Workplace-Name: College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, P.R. China Title: Changes in the expression of CrFTA, the Catharanthus roseus farnesyltransferase α-subunit gene, in response to a Candidatus Liberibacter asiaticus infection Abstract: The farnesyltransferase α-subunit (FTA) may be involved in the regulation of defence responses against pathogens in plants. In this study, this gene was amplified from Catharanthus roseus (CrFTA gene). The cDNA was found to be 1 403 bp long, and encodes a putative protein of 331 amino acids that contains a conserved PPTA motif. The phylogenetic analysis showed that the sequence of CrFTA is the most similar to that from Coffea canephora. The qRT-PCR assays indicated that CrFTA is expressed in the leaves, stems, and roots. During a Candidatus Liberibacter asiaticus (Ca. L. asiaticus) infection, the CrFTA expression levels significantly increased and reached 18-fold that measured in the control group, after which its expression decreased gradually from 22 days after top-grafting (DAT) to the end of the experiment. Spray application of Manumycin A (ManuA), a specific inhibitor of farnesyltransferase, on the leaves of C. roseus plants caused a significant decrease in the CrFTA expression and a significant increase in the Ca. L. asiaticus positivity percentage after top-grafting with the Ca. L. asiaticus-infected shoots compared with the groups not treated with ManuA. Furthermore, ABA had no significant effect on the relative expression of CrFTA and the number of Ca. L. asiaticus-positive plants. These results suggest that CrFTA most likely plays a role in mediating the tolerance to a Ca. L. asiaticus infection in C. roseus. Keywords: Ca. L. asiaticus, gene expression, Madagascar periwinkle, Manumycin A Journal: Czech Journal of Genetics and Plant Breeding Pages: 36-42 Volume: 57 Issue: 1 Year: 2021 DOI: 10.17221/13/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/13/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202101-0005.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:1:id:13-2020-CJGPB