Template-Type: ReDIF-Article 1.0 Author-Name: M.C. Narendra Author-Workplace-Name: Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India Author-Name: Chandan Roy Author-Workplace-Name: Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India Author-Name: Sudhir Kumar Author-Workplace-Name: Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India Author-Name: Parminder Virk Author-Workplace-Name: Harvest Plus, C/O International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Patancheru, Telengana, India Author-Name: Nitish De Author-Workplace-Name: Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India Title: Effect of terminal heat stress on physiological traits, grain zinc and iron content in wheat (Triticum aestivum L.) Abstract: Heat stress is one of the major wheat (Triticum aestivum) production constraints in South Asia (SA), particularly in the Eastern Gangetic Plains (EGP) of India and Bangladesh. Malnutrition is also a severe problem among children and women in SA. Wheat varieties with high grain Zn/Fe are a sustainable, cost-effective solution in the fight against hidden hunger. Thirty wheat genotypes were characterised under the optimum temperature and heat stress conditions in 2016-2017 and 2017-2018 to study the response of the stress on the yield, physiological traits and grain Zn/Fe content. A significant genetic variation was observed for all the traits under the optimum temperature and stress conditions. The yield was reduced by an average of 59.5% under heat stress compared to that of the optimum temperature. A strong positive association of the canopy temperature depression (CTD) with the grain yield (GY) was observed under the heat stress. A negative correlation of the grain Zn/Fe with the yield was observed under the optimum temperature and heat stress conditions, while the association between the grain Zn and Fe was positive. The genotypes BRW 3723, BRW 3759, BRW 3797, BRW 160, HD 2967, HD 2640 were found to be heat-tolerant in both years. Among the tolerant genotypes, BRW 934, BRW 3807 and BRW 3804 showed a high zinc content and BRW 934, BRW 3797, BRW 3788 and BRW 3807 showed a high iron content, respectively. These genotypes can be explored in future breeding programmes to address the problem of nutritional deficiency. Keywords: abiotic stress, biofortification, canopy temperature depression, peroxidase activity Journal: Czech Journal of Genetics and Plant Breeding Pages: 43-50 Volume: 57 Issue: 2 Year: 2021 DOI: 10.17221/63/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/63/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202102-0001.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:2:id:63-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jiping Tong Author-Name: Zhengshu Han Author-Workplace-Name: Crop Institute, Tianjin Academy of Agricultural Sciences, Tianjin, P.R. China Author-Name: Aonan Han Author-Workplace-Name: Crop Institute, Tianjin Academy of Agricultural Sciences, Tianjin, P.R. China Title: Genetic analysis and molecular mapping of Rp, a mutant gene encoding red pericarp in rice (Oryza sativa L.) Abstract: Coloured rice has pigments deposited in the grain pericarp; red rice is the most common type of coloured rice. Red rice is rich in essential nutrients and has been grown and consumed in China for a long time. In this study, we report the genetic characterisation and preliminary molecular mapping of a mutant gene encoding red pericarp in rice (Oryza sativa L.). To analyse the genetic basis of the red pericarp mutant, a reciprocal cross between GER-3 (red pericarp, indica cv.) and 898 (white pericarp, indica cv.) was made. The genetic analysis results confirmed that there was only one dominant gene, temporarily designated Rp (Red pericarp) controlling the segregation of the red pericarp in the F2 population. For the molecular mapping of Rp, an F2 population derived from an inter-subspecific cross between Gene Engineering Rice-3 (GER-3) and C418 (japonica cv., white pericarp) was constructed. The genotype of the pericarp colour of the F2 individuals in the mapping population was validated by progeny testing of the F2:3 families. Simple sequence repeat (SSR) markers and the bulked segregation analysis (BSA) method were used; Rp was mapped to the short arm of chromosome 7 between the SSR markers RM21182 and RM21268, with a genetic distance of 3.5 and 12.0 cM, respectively. In this paper, the potential origin of the red pericarp mutant gene Rp was also discussed. Keywords: gene mapping, genetic characteristics analysis, coloured rice, red pericarp mutant Journal: Czech Journal of Genetics and Plant Breeding Pages: 51-57 Volume: 57 Issue: 2 Year: 2021 DOI: 10.17221/70/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/70/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202102-0002.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:2:id:70-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mai Phuong Pham Author-Workplace-Name: Vietnam-Russia Tropical Centre, Hanoi, Vietnam Author-Name: Viet Ha Tran Author-Workplace-Name: College of Forestry, Vietnam National University of Forestry, Hanoi, Vietnam Author-Name: Dinh Duy Vu Author-Workplace-Name: Vietnam-Russia Tropical Centre, Hanoi, Vietnam Author-Workplace-Name: Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam Author-Name: Quoc Khanh Nguyen Author-Workplace-Name: Vietnam-Russia Tropical Centre, Hanoi, Vietnam Author-Name: Syed Noor Muhammad Shah Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan Title: Phylogenetics of native conifer species in Vietnam based on two chloroplast gene regions rbcL and matK Abstract: We used two chloroplast gene regions (matK and rbcL) as a tool for the identification of 33 local conifer species. All 136 sequences, 101 newly generated (14 species for gene matK; 16 species for gene rbcL) and 35 retrieved from the GenBank, were used in the analysis. The highest genetic distance (matK region) was recorded between the species in Cupressaceae with an average of 5% (0.1-8.5), Podocarpaceae with an average of 6% (0-8.5), Taxaceae with an average of 5% (0.2-0.5) and Pinaceae with an average of 20.4% (0.8-54.1). The rbcL region showed a low genetic distance between the species in Cupressaceae 2% (0-3.3), Podocarpaceae 3% (0.6-3.4), Taxaceae 1% (0-2.1) and Pinaceae 1.2% (0-5.82). The phylogenetic analyses using the Maximum likelihood (ML) and Bayesian inference (BI) bootstrap values obtained at the branching nodes of each species ranged from 62 to 100% (Maximum likelihood bootstrap - MLBS and Bayesian posterior probabilities - BPP) for the matK gene; from 66 to 100% (MLBS) and 60 to 100% (BPP) for the rbcL region. The rbcL region was not identified between the species of Taxaceae and Cephalotaxaceae. The matK gene region was very clear in the different species among the families (Cupressaceae, Podocarpaceae, and Cephalotaxaceae) and unsuitable for identifying closely related species in Amentotaxus (Taxaceae) and Pinus (Pinaceae). The gene (matK) is a useful tool as a barcode in the identification of conifer species of Cupressaceae, Podocarpaceae, and Cephalotaxaceae in Vietnam. Keywords: DNA barcoding, endangered, phylogeny, taxonomy Journal: Czech Journal of Genetics and Plant Breeding Pages: 58-66 Volume: 57 Issue: 2 Year: 2021 DOI: 10.17221/88/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/88/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202102-0003.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:2:id:88-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Kateřina Baránková Author-Workplace-Name: Mendeleum Department, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: Anna Nebish Author-Workplace-Name: Institute of Grapevine and Wine Sciences (ICVV, UR-CSIC-GR), Logroño, La Rioja, Spain Author-Workplace-Name: Department of Genetics and Cytology, Yerevan State University, Yerevan, Armenia Author-Name: Jan Tříska Author-Workplace-Name: Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Institute CAS, Brno, Czech Republic Author-Name: Jana Raddová Author-Workplace-Name: Mendeleum Department, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: Miroslav Baránek Author-Workplace-Name: Mendeleum Department, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Title: Comparison of DNA methylation landscape between Czech and Armenian vineyards show their unique character and increased diversity Abstract: Grapevine is a worldwide crop and it is also subject to global trade in wine, berries and grape vine plants. Various countries, including the countries of the European Union, emphasize the role of product origin designation and suitable methods are sought, able to capture distinct origins. One of the biological matrices that can theoretically be driven by individual vineyards' conditions represents DNA methylation. Despite this interesting hypothesis, there is a lack of respective information. The aim of this work is to examine whether DNA methylation can be used to relate a sample to a given vineyard and to access a relationship between a DNA methylation pattern and different geographical origin of analysed samples. For this purpose, DNA methylation landscapes of samples from completely different climatic conditions presented by the Czech Republic (Central Europe) and Armenia (Southern Caucasus) were compared. Results of the Methylation Sensitive Amplified Polymorphism method confirm uniqueness of DNA methylation landscape for individual vineyards. Factually, DNA methylation diversity within vineyards of Merlot and Pinot Noir cultivars represent only 16% and 14% of the overall diversity registered for individual cultivars. On the contrary, different geographical location of the Czech and Armenian vineyards was identified as the strongest factor affecting diversity in DNA methylation landscapes (79.9% and 70.7% for Merlot and Pinot Noir plants, respectively). Keywords: authentication, grapevine cultivar, geographical origin, plant adaptation, epigenetic changes, methylation sensitive amplified polymorphism (MSAP) Journal: Czech Journal of Genetics and Plant Breeding Pages: 67-75 Volume: 57 Issue: 2 Year: 2021 DOI: 10.17221/90/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/90/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202102-0004.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:2:id:90-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Salem Marzougui Author-Workplace-Name: Regional Office of Agricultural Research Development, El-Kef, Tunisia Author-Workplace-Name: Field Crops Laboratory, INRAT, University of Carthage, Tunisia Title: Allelic variations at the HvSNF2 and HvBM5 loci are associated with the heading date and growth habit of barley (Hordeum vulgare L.) under a semi-arid climate Abstract: The heading date and growth habit are key factors that regulate the transition from the vegetative to the reproductive stage in barley. In this study, we used PCR based markers to identify the allelic variations in the Vrn-H1 (HvMB5) and Vrn-H2 (HvSNF2) genes and to predict the heading date and growth habit of a collection of Tunisian barley assessed under a semi-arid climate. The allelic variation at HvBM5 revealed two PCR fragments at 830 and 344 bp. Primer sets used to amplify the HvSNF2 gene have resulted in different alleles size of 543, 623, and 700 bp. Different allelic combinations of HVBM5 and HvSNF2 were associated with the heading date and growth habit. The spring and early heading accessions were only characterised by the amplification of the HvSNF2 fragment at 700 bp. All the winter accessions yielded the PCR product HvBM5 at 830 bp, but the variation in the heading date was determined by the HvSNF2 alleles. These DNA markers will be a powerful tool to predict the heading date and growth habit and can be used as markers for the assisted selection to speed up the national breeding programme. Keywords: allelic combinations, days to heading, growth habit, long-day conditions, vernalisation, Vrn-H loci Journal: Czech Journal of Genetics and Plant Breeding Pages: 76-79 Volume: 57 Issue: 2 Year: 2021 DOI: 10.17221/62/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/62/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202102-0005.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:2:id:62-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tomáš Mezlík Title: List of field crop varieties registered in the Czech Republic in 2020 Journal: Czech Journal of Genetics and Plant Breeding Pages: 80-81 Volume: 57 Issue: 2 Year: 2021 DOI: 10.17221/13/2021-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/13/2021-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:2:id:13-2021-CJGPB