Template-Type: ReDIF-Article 1.0 Author-Name: Gang Wei Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Hongxia Yang Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Zixian Xiong Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Jingwen Wu Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Danyang Chen Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Yang Liu Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Yijie Ban Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Weichi Liu Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Lina Shang Author-Workplace-Name: Potato Crop Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Author-Name: Nan Wang Author-Workplace-Name: Rice Research Institute, College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R. China Title: TPR domain coding gene ST2 may be involved in regulating tillering and fertility in rice Abstract: A decrease in the tiller number and male sterility will lead to a decline in the rice yield. Therefore, it is significant to study the molecular mechanism of controlling the tiller number and regulating the male reproductive development. The mutant st2 (single tiller 2) was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B and showed single tillering and male sterility. I2-KI staining showed that the st2 pollen was aborted. The scanning electron microscope (SEM) observation underlined that the anther of st2 became smaller, the wax of the epidermis reduced, the inner wall shrank and the Ubisch body decreased, the pollen collapsed, and the germination pore developed abnormally. The genetic analysis discovered that the trait was controlled by a single recessive nuclear gene located on chromosome 3. LOC_Os03g05540 encoding a tetratricopeptide repeat (TPR) domain was identified as the candidate gene by sequencing. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that ST2 was highly expressed in the stem apical meristem (SAM) and the initial stage of meiosis during the anther development. The subcellular localisation indicated that ST2 is a nuclear and plasmic localisation protein. The homology analysis demonstrated that ST2 was evolutionarily conserved. These results laid a foundation for further study of the ST2 function. Keywords: gene mapping, male sterility, Oryza sativa L., tiller, tetratricopeptide repeat (TPR) Journal: Czech Journal of Genetics and Plant Breeding Pages: 83-90 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/103/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/103/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202103-0001.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:103-2020-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Xiaocui Yan Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Takele-Weldu Gebrewahid Author-Workplace-Name: College of Agriculture, Aksum University, Shire-Indaslassie, Tigray, Ethiopia Author-Name: Rui Dong Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Xing Li Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Peipei Zhang Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Name: Zhanjun Yao Author-Workplace-Name: College of Agronomy, Hebei Agricultural University, North China Key Laboratory for Crop Germplasm Resources of China's Education Ministry, Baoding, Hebei, P.R. China Author-Name: Zaifeng Li Author-Workplace-Name: Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Title: Identification of known leaf rust resistance genes in bread wheat cultivars from China Abstract: Leaf rust caused by Puccinia triticina Eriks. (Pt) is one of the most devastating fungal pathogens affecting wheat (Triticum aestivum L.) production worldwide. Deployment of resistant cultivars is the most environmentally friendly approach to control the disease. In this study, thirty-seven wheat lines from the Hubei and Shaanxi provinces in China were evaluated for seedling resistance in the greenhouse using eighteen Pt races. These lines were also tested for slow rusting resistance in the field in the 2014 to 2018 growing seasons. Eleven molecular markers closely associated with known Lr genes were used as part of the postulation process. Seven known Lr genes, 1, 13, 18, 14a, 26, 34 and 46 either singly or in combination were postulated in twenty-five cultivars. Lr1 and Lr26 were the most commonly identified genes detected in thirteen and ten cultivars, respectively. Lr13 and Lr46 were each found in four and five cultivars. Lr34 was present in three cultivars. Lr18 and Lr14a were identified in cultivar Xi'nong 538. Six cultivars displayed slow rusting resistance in the field tests. The resistant cultivars identified in the present study can be used as resistance parents in crosses aimed at pyramiding and the deployment of leaf rust resistance genes in China. Keywords: adult plant resistance, gene postulation, molecular markers, Triticum aestivum Journal: Czech Journal of Genetics and Plant Breeding Pages: 91-101 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/6/2021-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/6/2021-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202103-0002.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:6-2021-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zhijun Tong Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Sanjie Jiang Author-Workplace-Name: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Name: Weiming He Author-Workplace-Name: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Name: Xuejun Chen Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Lixin Yin Author-Workplace-Name: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Name: Dunhuang Fang Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Yafei Hu Author-Workplace-Name: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Name: Fangchan Jiao Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Chi Zhang Author-Workplace-Name: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Name: Jianmin Zeng Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Xinfu Wu Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Author-Name: Shancen Zhao Author-Workplace-Name: BGI Institute of Applied Agriculture, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Name: Jianbo Jian Author-Workplace-Name: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China Author-Workplace-Name: Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark Author-Name: Bingguang Xiao Author-Workplace-Name: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China Title: Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing Abstract: Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants. Keywords: crossing, single nucleotide polymorphism (SNP), whole genome sequencing (WGS) Journal: Czech Journal of Genetics and Plant Breeding Pages: 102-112 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/8/2021-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/8/2021-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202103-0003.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:8-2021-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jiping Tong Author-Name: Zhengshu Han Author-Workplace-Name: Crop Institute, Tianjin Academy of Agricultural Sciences, Tianjin, P.R. China Author-Name: Aonan Han Author-Workplace-Name: Crop Institute, Tianjin Academy of Agricultural Sciences, Tianjin, P.R. China Title: Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL population Abstract: Anthocyanin pigmentation is an important morphological marker that is commonly used to identify rice varieties and for linkage analysis. The following study investigates the genetic factors involved in the purple stigma (Ps) and purple apiculus (Pa) traits of an important indica rice cross between Zhenshan 97 (purple stigma and purple apiculus) and Minghui 63 (grey stigma and colourless apiculus). A recombinant inbred line (RIL) population derived from this cross was used for quantitative trait loci (QTL) mapping of the purple stigma and purple apiculus traits. As a result, one major QTL for the purple stigma trait, temporarily designated qPS-1-1, and one major QTL for the purple apiculus trait, temporarily designated qPA-1-1, were mapped to the short arm of chromosome 6 in the interval between the two markers Y4073L and *P. The LOD peaks of qPS-1-1 and qPA-1-1 were 44.0127 and 173.3585, respectively. In addition, qPS-1-1 and qPA-1-1 explained 66.7416% and 98.6441% of the total phenotypic variance, respectively. The Zhenshan 97 allele increased the purple stigma trait by approximately 8.0355% (for qPS-1-1) and 9.8863% (for qPA-1-1). Moreover, since qPS-1-1 and qPA-1-1 were strongly correlated, they were also located in the same vicinity of the C gene on the short arm of chromosome 6, which suggested that the two QTL might be the same. By comparing these and previous results, it was deduced that qPS-1-1 or qPA-1-1 was the C gene and was pleiotropic for both the colouration of the apiculus and the colouration of the stigma in rice. Keywords: colouration of the stigma and apiculus, QTL mapping, recombinant inbred lines (RILs), rice (Oryza sativa L.) Journal: Czech Journal of Genetics and Plant Breeding Pages: 113-118 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/20/2021-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/20/2021-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202103-0004.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:20-2021-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lei Zhu Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Yanman Li Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Jintao Li Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Yong Wang Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Zhenli Zhang Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Yanjiao Wang Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Zanlin Wang Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Jianbin Hu Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Luming Yang Author-Workplace-Name: College of Horticulture, Henan Agricultural University, Zhengzhou, P.R. China Author-Name: Shouru Sun Title: Genome-wide identification and analysis of the MLO gene families in three Cucurbita species Abstract: Powdery mildew (PM) is a major fungal disease in the Cucurbita species in the world, which can cause significant yield loss. The Mildew Locus O (MLO) family genes play important roles in the PM stress response. In this paper, twenty, twenty-one, and eighteen candidate MLO genes in Cucurbita moschata, Cucurbita maxima and Cucurbita pepo, respectively, were identified and designated as CmoMLO, CmaMLO and CpeMLO, respectively. The phylogenetic analysis indicated that these MLOs were divided into five clades and the number of MLOs belonging to clade V in the Cucurbita species was more than that in other crops. Furthermore, the expression analysis in the susceptibility (S) and resistance (R) lines showed that CpeMLO1, CpeMLO2 and CpeMLO5 might be involved in the susceptibility response. CpeMLO4 and CpeMLO6 showing opposite expression patterns in the R/S lines might be involved in the resistance response. All these data would be beneficial for future functional analysis of MLOs in the Cucurbita species. Keywords: Cucurbita maxima, Cucurbita moschata, Cucurbita pepo, expression analysis, phylogenetic analysis, powdery mildew, resistance response, susceptibility response Journal: Czech Journal of Genetics and Plant Breeding Pages: 119-123 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/99/2020-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/99/2020-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202103-0005.txt Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:99-2020-CJGPB