Template-Type: ReDIF-Article 1.0 Author-Name: Anamaria Mirabela Ilina Dumitru Author-Workplace-Name: Doctoral School of Plant and Animal Resources Engineering, Faculty of Horticulture, University of Craiova, Craiova, Romania Author-Workplace-Name: National Research and Development Institute for Biotechnology in Horticulture - Ștefăneşti, Ștefăneşti, Argeş, Romania Author-Name: Andreea Elena Manolescu Author-Workplace-Name: National Research and Development Institute for Biotechnology in Horticulture - Ștefăneşti, Ștefăneşti, Argeş, Romania Author-Name: Dorin Ioan Sumedrea Author-Workplace-Name: National Research and Development Institute for Biotechnology in Horticulture - Ștefăneşti, Ștefăneşti, Argeş, Romania Author-Name: Carmen Florentina Popescu Author-Name: Sina Cosmulescu Title: Genetic diversity of some autochthonous white grape varieties from Romanian germplasm collections Abstract: We investigated the phenotypic and genetic diversity among 25 grapevine accessions of white grape maintained in a germplasm collection ex situ. The selected varieties were characterised using standardised ampelographic descriptors and microsatellite markers to obtaintheir complete description. The ampelographic analysis based on 24 OIV descriptors and the obtained first dendrogram based on common features, revealed the extent of the phenotypic diversity ranging from 0.29 (between Coarnă albă and Chardonnay) to 0.92 (between Crâmpoşie and Cioinic), without any connection with their supposed origin or the current geographic area of distribution. The SSR allelic analysis with nine standard markers proved and confirmed its high discrimination potential for genotype identification and diversity. The second cluster analysis aimed to evaluate the genetic relationships among analysed accessions,based on Dice's similarity coefficient, revealed two groups of varieties: the first group (A) included twelve accessions with a degree of similarity between 0.37 and 0.5. The second (B) included eleven accessions, with a similarity degree between 0.33 and 0.67. The genotypic cluster analysis showed a distinct lower similarity between the Majarcă albă variety and all the varieties from the first group, and between Busuioacă de Bohotin and all the analysed varieties. The phenotypic and genetic matrices and dendrograms, although not correlated, were complimentary and provided valuable information about the diversity of the grapevine genotypes and the identification of the synonyms. Keywords: genetic variability, molecular markers, phenotypic traits, similarity coefficient, Vitis vinifera L Journal: Czech Journal of Genetics and Plant Breeding Pages: 55-66 Volume: 59 Issue: 2 Year: 2023 DOI: 10.17221/45/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/45/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202302-0001.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:2:id:45-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Neha Verma Author-Workplace-Name: Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Karmvir Singh Garcha Author-Workplace-Name: Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Madhu Sharma Author-Workplace-Name: Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Abhishek Sharma Author-Workplace-Name: Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India Author-Name: Ajmer Singh Dhatt Title: Gene effects for begomovirus resistance and plant architecture attributes in pumpkin (Cucurbita moschata Duchesne) Abstract: Knowledge of gene actions governing begomovirus resistance and plant architectural traits is a prerequisite for a successful hybrid breeding programme. Therefore, the gene actions associated with these traits were studied in two intervarietal crosses of Cucurbita moschata (C1: Punjab Nawab × MVSR-6711 and C2: Punjab Nawab × P-135). We used the generation mean analysis of six generations for this purpose. Significant differences between the generation means were observed for all the traits in both crosses. The parental lines differed significantly in most of the studied traits. The nature and magnitude of the gene effects of seventeen traits varied by trait and cross. A simple additive dominance model was adequate for the internode number, leaf length and width, petiole length, fruit weight and cavity diameter in C1 and the number of fruits/plant in C2. The non-allelic interaction was found to be significant for a majority of the traits including the per cent disease index of the squash leaf curl China virus, tomato leaf curl New Delhi virus and their mixed infections, which indicated, that recurrent selection in biparental progeny might be useful for the accumulation of genes with additive effects. Duplicate epistasis was observed for the vine, internodal and peduncle length in C1 and the internode number, petiole and peduncle length, peduncle and fruit polar diameter in C2. This information will help to establish a breeding program for the simultaneous improvement of virus resistance and yield traits in pumpkins. Keywords: gene action, SLCCNV, scaling test, ToLCNDV, yield Journal: Czech Journal of Genetics and Plant Breeding Pages: 67-75 Volume: 59 Issue: 2 Year: 2023 DOI: 10.17221/56/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/56/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202302-0002.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:2:id:56-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mostafa Alamholo Author-Workplace-Name: Department of Biotechnology, Institute of Science and Modern Technology, Rojava University, Qamishlo, Syria Author-Name: Alireza Tarinejad Title: Molecular mechanism of drought stress tolerance in barley (Hordeum vulgare L.) via a combined analysis of the transcriptome data Abstract: One of the main issues addressed by phytology in recent years has been plant tolerance mechanisms for abiotic stress. No combined analysis has been made to identify the genes involved in drought stress tolerance. The meta-analysis of microarray data related to drought stress was analysed by the R software packages and showed 3 029 upregulated genes and 3 017 downregulated genes. The upregulated genes were mostly related to the drought tolerance protein, abiotic stress response, and the Cys2His2 Zinc Finger Transcription Factor (C2H2 zinc finger TF). The downregulated genes were mainly related to the late embryogenesis abundant protein, abiotic stress response, and the basic leucine zipper (bZIP) TF. The common gene ontology (GO) terms in the upregulated and downregulated genes were mainly related to the metabolic process, response to stimulus, cellular metabolic process, and photorespiration. The up and down meta-differential expressed genes (meta-DEGs) mainly belonged to the those following Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including: the biosynthesis of secondary metabolites, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signalling pathway, and RNA degradation. Moreover, in the upregulated and downregulated genes, the TFs with a high percentage mainly belonged to the Teosinte branched1/Cincinnata/proliferating cell factor (TCP), basic helix loop-helix (bHLH) and bZIP. Next, the hub upregulated genes were mainly related to the thiamine biosynthesis protein thiC, 4-hydroxyphenylpyruvate dioxygenase, ribose-5-phosphate isomerase precursor and heat shock protein. The hub downregulated genes were mainly associated with the elongation factor Ts, aldehyde dehydrogenase, and trigger factor. Finally, the data from the present meta-analysis were compared with previous studies on the qRT-PCR results and their up and down expressions were confirmed. Based on the findings of the current study, novel insights into the drought stress molecular response can be provided and various candidate genes can be introduced for barley drought stress tolerance breeding. Keywords: bioinformatics, biological process, drought stress, hub genes, meta-analysis, transcription factor Journal: Czech Journal of Genetics and Plant Breeding Pages: 76-94 Volume: 59 Issue: 2 Year: 2023 DOI: 10.17221/69/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/69/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202302-0003.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:2:id:69-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Yan Wang Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China Author-Name: Xiaohua Yao Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Name: Youhua Yao Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China Author-Name: Yixiong Bai Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China Author-Name: Likun An Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China Author-Name: Xin Li Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China Author-Name: Yongmei Cui Author-Workplace-Name: Qinghai University, Xining, P.R. China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China Author-Name: Kunlun Wu Author-Workplace-Name: Qinghai University, Xining, P.R. China Title: QTL localisation of seed-related traits in Tibetan hulless barley based on a high-density single-nucleotide polymorphism genetic map Abstract: The effective use of high-quality and high-yielding germplasm resources is of great importance for the development of hulless barley varieties. Therefore, the search for such resources has long been a goal of the breeding community. In this study, a genotyping-by-sequencing (GBS) analysis was performed on an F2 (Nierumuzha × Kunlun 10) population. A high-density genetic map of hulless barley was constructed, which contained 1 475 efficient single-nucleotide polymorphism markers with 7 052 bin markers. The total length of the seven chromosomes was 1 139.4 cM, with an average marker distance of 0.59 cM. Based on this high-density linkage map, a total of 54 quantitative trait loci (QTLs) related to the seed traits were detected, including seed colour (SC), thousand kernel weight (TKW), seed average area (SAA), seed perimeter (SP), seed length (SL), seed width (SW), seed length/width (SLW), seed diameter (SD), and seed circular degree (SCD). These QTLs explained 3.32-35.73% (mean = 11.45%) of the phenotypic interpretation, including 24 main QTLs and 30 epistatic QTLs. A total of 24 candidate genes were identified within the QTL region, including one SC-associated transcription factor (ANT1), two TSW-related genes, a transcription factor (NAC021), a gene associated with the non-homologous end joining (NHEJ) pathway (ku70), three SAA-associated genes (LOL2, NAC021, TSK), two SL-associated genes (MADS21, MADS4), six SW-associated genes (FIP1, NAC021, DREB 1A, HVA22A, CYP78A6, SAUR71), five LW-related genes (NAM-B2, CRY1, LHY, CYP710A1, WRKY72), two SP-related genes (SKIP11, TCP18), two SD-related genes (NAC021, SKIP8), and three SCD-related genes (MYB1R1, RAX3, NAC100). These genes are involved in the regulation of the cell development, material transport, signal transduction, and plant morphogenesis and play an important role in the regulation of agronomic traits in hulless barley. The high-density genetic mapping and QTL identification of the seed traits in hulless barley provide a valuable genetic resource and the basis for further molecular marker-assisted selection and genomic studies. Keywords: genotyping-by-sequencing (GBS), linkage analysis, quantitative trait loci, seed traits Journal: Czech Journal of Genetics and Plant Breeding Pages: 95-108 Volume: 59 Issue: 2 Year: 2023 DOI: 10.17221/74/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/74/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202302-0004.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:2:id:74-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Kunwar Harendra Singh Author-Workplace-Name: ICAR - Directorate of Rapeseed Mustard Research, Bharatpur, Rajasthan, India Author-Name: Guman Singh Author-Workplace-Name: ICAR - Directorate of Rapeseed Mustard Research, Bharatpur, Rajasthan, India Author-Name: Lal Singh Author-Workplace-Name: ICAR - Directorate of Rapeseed Mustard Research, Bharatpur, Rajasthan, India Author-Name: Nashra Aftab Author-Workplace-Name: ICAR - Directorate of Rapeseed Mustard Research, Bharatpur, Rajasthan, India Author-Name: Ajay K. Thakur Title: Delineation of inbred lines of Indian mustard into diverse gene pools based on agro-morphological traits Abstract: The present study was conducted to explore the variability generated through recombination breeding for nine economically important traits in Indian mustard (Brassica juncea L. Czern & Coss.). Delineation of the inbred lines to different gene pools, based on genetic diversity, enables their utilisation in hybrid breeding. The unweighted pair group method with arithmetic mean (UPGMA) and a Euclidean distance matrix was used to delineate the inbred lines to clusters. The variability was studied using the range and coefficients of variation of the traits. Significant variability was observed for all studied traits except for oil content and days to maturity. 128 Indian mustard genotypes were grouped into four distinct gene pools based on genetic diversity. A set of 20 most diverse genotype combinations was produced. Promising inbred lines were identified and recommended as donors for the respective trait. The pedigree analysis of the inbred line groups revealed, that recombination breeding caused a large diversity as confirmed by the assignment of inbred lines with the same parentage to specific clusters. Keywords: Brassica juncea, characterization, genetic diversity Journal: Czech Journal of Genetics and Plant Breeding Pages: 109-116 Volume: 59 Issue: 2 Year: 2023 DOI: 10.17221/75/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/75/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202302-0005.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:2:id:75-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tomáš Mezlík Author-Workplace-Name: National Plant Variety Office, Central Institute for Supervising and Testing in Agriculture, Brno, Czech Republic Title: List of Field Crop Varieties Registered in the Czech Republic in 2022 Journal: Czech Journal of Genetics and Plant Breeding Pages: 117-118 Volume: 59 Issue: 2 Year: 2023 DOI: 10.17221/22/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/22/2023-CJGPB.html File-Format: text/html Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:2:id:22-2023-CJGPB