Template-Type: ReDIF-Article 1.0 Author-Name: Xiang Fang Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Author-Name: Yiling Lin Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Author-Name: Chun Chen Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Author-Name: Tariq Pervaiz Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Author-Name: Xicheng Wang Author-Workplace-Name: Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P.R. China Author-Name: Hefei Luo Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Author-Name: Jinggui Fang Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Author-Name: Lingfei Shangguan Author-Workplace-Name: Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, P.R. China Author-Workplace-Name: Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, P.R. China Title: Whole genome identification of CBF gene families and expression analysis in Vitis vinifera L. Abstract: The CBF (C-repeat binding factors) genes play important roles in response to abiotic stress and environmental changes. In the present study, a total of 18 CBF genes were identified from a grapevine. Their domains, phylogenetics, and collinearity were analysed. The results revealed, that 18 VviCBF genes were distributed on 10 chromosomes unevenly in the grape genome. Promoter data analysis showed that the CBF gene has many cis-acting elements related to plant growth and development, light response, hormone, and abiotic stress response. We found that six VviCBF genes including, VviCBF5, VviCBF13, VviCBF14, VviCBF15, VviCBF16, and VviCBF18 differentially expressed during fruit developmental stages. Furthermore, four VviCBF genes including, VviCBF1, VviCBF3, VviCBF6, and VviCBF11 were expressed at the early stage of bud dormancy, whereas, nine VviCBF genes were expressed at the bud dormancy-breaking stage. Additionally, various VviCBFs genes respond to different abiotic and biotic stress. These findings will lay a foundation for further study of the CBF genes in bud dormancy, downy mildew, and abiotic and biotic stresses. Keywords: biotic and abiotic stresses, bud dormancy, CBF, fruit development, grape Journal: Czech Journal of Genetics and Plant Breeding Pages: 119-132 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/82/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/82/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0001.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:82-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jia Liu Author-Workplace-Name: Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, PR China Author-Name: Jin Zhu Author-Workplace-Name: Sichuan Horticultural Crop Technology Promotion Station, Chengdu, P.R. China Author-Name: Hongwen Li Author-Workplace-Name: Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, PR China Author-Name: Deyong Luo Author-Workplace-Name: Sichuan Fruit Tree Breeding Station, Sichuan, PR China Author-Name: Jialian Xie Author-Workplace-Name: Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Key Laboratory of Integrated Management of Pests on Crops in Southwest China, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan, P.R. China Author-Name: Huajia Li Author-Workplace-Name: Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, P.R. China Author-Name: Shuo Liu Author-Workplace-Name: Liaoning Institute of Pomology, Yingkou, Liaoning, P.R. China Author-Name: Yuping Zhang Author-Workplace-Name: Liaoning Institute of Pomology, Yingkou, Liaoning, P.R. China Author-Name: Lijuan Chen Author-Workplace-Name: Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, PR China Author-Name: Xiaodou Xie Author-Workplace-Name: Sichuan Fruit Tree Breeding Station, Sichuan, PR China Author-Name: Dong Wang Author-Workplace-Name: Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, PR China Author-Name: Ke Li Author-Workplace-Name: Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, P.R. China Author-Name: Meiying Yao Author-Workplace-Name: Panzhihua Academy of Agriculture and Forestry Sciences, Panzhihua, Sichuan, PR China Author-Name: Guowei Zhang Author-Workplace-Name: Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, PR China Title: A preliminary study on the root-knot nematode resistance of a cherry plum cultivar Mirabolano 29C Abstract: Grafting is the most important means of breeding peach, plum, apricot, and other fruit trees, and the selection of the rootstock is crucial to the quality of the grafting and the yield of the products. The traditional commonly used peach rootstock is susceptible to root-knot nematode infections, resulting in a decreased yield, while a variety of cherry plum rootstocks, Mirabolano 29C, is resistant to root-knot nematode. In this study, root-knot nematode infection experiments on seedlings of traditional peach rootstocks and Mirabolano 29C confirmed that Mirabolano 29C was indeed more resistant to root-knot nematodes. At the same time, we compared the roots of the root-knot nematode uninfected and infected Mirabolano 29C by transcriptome sequencing and found 3 176 differentially expressed genes. A further functional enrichment analysis of these genes found that the secondary metabolites, phenylpropane and flavonoids, may be responsible for the high resistance of Mirabolano 29C to root-knot nematodes. These results can provide a reference value for the disease resistance breeding of rootstocks. Keywords: disease resistance, grafting, phenylpropane pathway, rootstocks, transcriptome sequencing Journal: Czech Journal of Genetics and Plant Breeding Pages: 133-140 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/111/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/111/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0002.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:111-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Ivona Žďárská Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Radek Čmejla Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic Title: Effect of long-term storage on the change in the expression of selected Mal d 1 gene isoforms in the apple cultivar Opal® Abstract: Apples are available all year round since they can be stored for long time. However, the expression of the major apple allergen Mal d 1 can increase in the fruit during storage and incease so the risk of allergies in sensitive consumers. Therefore, we studied the change in the expression of the Mal d 1 gene during storage in the modern cultivar UEB 32642 (known under the brand name Opal®) and the cultivar Gala Brookfield (cv. Gala) as a reference. The cultivars were stored under two different conditions, ultra-low oxygen (ULO) and standard cold conditions. The gene expression was evaluated both in fresh fruits and in fruits stored for four, six, and nine months. Nine isoforms of the gene Mal d 1 with the highest published expression were quantified using real-time PCR. The most expressed isoforms Mal d 1.01, Mal d 1.02 and Mal d 1.06A were found in the fresh and also in the stored fruits. The expression of the Mal d 1.03G and Mal d 1.06D isoforms was higher the sored fruits. Our study confirmed that (i) Opal® had a lower overall expression of the Mal d 1 gene than cv. Gala, both in the fresh and stored fruits; (ii) standard cold storage is superior in preserving lower Mal d 1 levels in Opal®apples compared to the ULO conditions; and (iii) less expressed isoforms may be responsible for the general increase in the Mal d 1 gene expression during storage. Keywords: apple allergy, cold storage, controlled atmosphere, Malus × domestica Borkh., relative quantification Journal: Czech Journal of Genetics and Plant Breeding Pages: 141-147 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/102/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/102/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0003.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:102-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Nawroz Tahir Author-Workplace-Name: Horticulture Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani, Iraq Author-Name: Djshwar Lateef Author-Name: Kamaran Rasul Author-Workplace-Name: Horticulture Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani, Iraq Author-Name: Didar Rahim Author-Workplace-Name: Biotechnology and Crop Sciences Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani, Iraq Author-Name: Kamil Mustafa Author-Workplace-Name: Biotechnology and Crop Sciences Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani, Iraq Author-Name: Shokhan Sleman Author-Workplace-Name: Biotechnology and Crop Sciences Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani, Iraq Author-Name: Avin Mirza Author-Workplace-Name: General Directorate of Agriculture in Sulaimani, Ministry of Agricultural and Water Resources, Sulaimani, Iraq Author-Name: Rebwar Aziz Author-Workplace-Name: Horticulture Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani, Iraq Title: Assessment of genetic variation and population structure in Iraqi barley accessions using ISSR, CDDP, and SCoT markers Abstract: The objective of this study was to investigate the diversity of 59 accessions of barley using inter simple sequence repeat (ISSR), conserved DNA-derived polymorphism (CDDP), and start codon targeted (SCoT) markers. A total of 391 amplified polymorphic bands were generated using 44 ISSR, 9 CDDP, and 12 SCoT primers that produced 255, 35, and 101 polymorphic bands, respectively. The average values of gene diversity were 0.77, 0.67, and 0.81 for ISSR, CDDP, and SCoT markers, respectively. The mean values of polymorphism information content for ISSR, CDDP and SCoT markers were 0.74, 0.63, and 0.80 respectively. The discrimination power of the three approaches for assessing allelic diversity in barley accessions ranked as follows: SCoT > ISSR > CDDP. The barley accessions were classified and clustered into two main groups. Molecular variance analysis revealed 15, 9, and 14% variability among populations with ISSR, CDDP, and SCoT markers, respectively. The Mantel test results revealed that the three molecular marker matrices had significant positive relationships. The SCoT markers might be useful tools for selecting appropriate parents for a breeding program. Keywords: clustering, diversity, genetic differentiation, Hordeum vulgare, molecular markers Journal: Czech Journal of Genetics and Plant Breeding Pages: 148-159 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/112/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/112/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0004.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:112-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Yuling Ren Author-Workplace-Name: College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai, P.R. China Author-Name: Yajun Ma Author-Workplace-Name: College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, P.R. China Author-Name: Xue Li Author-Workplace-Name: College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai, P.R. China Author-Name: Xiaoan Li Author-Workplace-Name: College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai, P.R. China Author-Name: Guozhu Yang Author-Workplace-Name: Ecological Environment Engineering Research Center, Qinghai University, Xining, Qinghai, P.R. China Author-Name: Ping Li Author-Workplace-Name: College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai, P.R. China Title: Complete chloroplast genome sequence and characteristics analysis of Qingda no.1 alfalfa (Medicago sativa L. cv. Qingda no.1) Abstract: Medicago sativa is the most widely cultivated forage legume and one of the most economically valuable crops throughout the world. Qingda no.1 (Medicago sativa L. cv. Qingda no.1) is an excellent alfalfa local variety with strong cold, drought and salt resistance in the three rivers source area of Qinghai. In this study, the whole chloroplast (cp) genome of Qingda no.1 was sequenced, assembled and its structure was analysed by the Illumina high-throughput sequencing technology. The results showed that the chloroplast genome of Qingda no.1 exhibits no obvious typical quadripartite structure; the total length of the chloroplast genome is 125 637 bp; the chloroplast genome contained 111 genes, including 77 protein-coding genes, 30 tRNA genes, and 4 rRNA genes, with an overall GC content of 38.33%. The relative synonymous codon usage showed that 68.67% of the codons RSCU > 1 in Qingda no.1, with the preference ending with A and T. The simple sequence repeat (SSR) analysis identified 62 SSR loci. The phylogenetic analysis of the cp genome, Qingda no.1 clustered closely with Medicago sativa KU321683 (Medicago sativa L. cv. KU321683). These results are helpful for the further study of the Qingda no.1 adaptation mechanism to high altitude stress environments. Keywords: chloroplast genome sequencing, Medicago sativa L. cv. Qingda no.1, phylogenetic analysis, Qinghai-Tibet Plateau Journal: Czech Journal of Genetics and Plant Breeding Pages: 160-168 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/71/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/71/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0005.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:71-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Dyah Retno Wulandari Author-Name: Andri Fadillah Martin Author-Workplace-Name: Research Center of Genetic Engineering, National Research and Inovation Agency, National Integrated Center for Genomic, Tropical Biodiversity and Environment, Cibinong-Bogor, West Java, Indonesia Author-Name: Tri Muji Ermayanti Author-Workplace-Name: Research Center of Genetic Engineering, National Research and Inovation Agency, National Integrated Center for Genomic, Tropical Biodiversity and Environment, Cibinong-Bogor, West Java, Indonesia Author-Name: Khalisa Aini Sinaga Author-Workplace-Name: Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, West Java, Indonesia Author-Name: Diah Ratnadewi Author-Workplace-Name: Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, West Java, Indonesia Title: Morpho-genetic characterization of diploid and tetraploid taro (Colocasia esculenta L. Schott) cv. Kaliurang - An Indonesian local cultivar Abstract: Kaliurang is one of the local taro cultivars in Indonesia, potentially due to its high productivity and delicacy. This study analysed morpho-genetic variation between one diploid and three tetraploid Kaliurang taro clones from in vitro polyploid induction after plantlet acclimatization in the greenhouse for 6 weeks. Plants' morphological characters were analysed using the unweighted pair group method with arithmetic mean (UPGMA) method based on 50 characters according to the taro descriptor from the International Plant Genetic Resources Institute (IPGRI). Furthermore, a morphometric characterization based on the Euclidean distance of 17 digitations from mature leaves, was analysed with the Analysis Phylogenic and Evolution package in R software. DNA band pattern was also performed with ISSR to analyse the clone's genetic variance. The results showed morpho-genetic character diversity in diploid and tetraploid plants. A plant morphological study revealed that 22 out of 50 characters were distinct, with similarity coefficients ranging from 0.35 to 0.78. Leaf geometric morphometric analysis showed changes in five digitations and formed three clusters. Twelve ISSR primers out of twenty amplified the sequence of the Kaliurang taro genome to yield two clusters with a similarity coefficient of 0.71-0.83. Based on these three characterization approaches, tetraploid clones (K2 and K3) consistently differed morphologically from diploid clones (K0). Keywords: clones, clusters, ISSR, morphology, morphometric Journal: Czech Journal of Genetics and Plant Breeding Pages: 169-175 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/97/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/97/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0006.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:97-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Shashi Kumar Gupta Author-Workplace-Name: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India Author-Name: Ponnaiah Govintharaj Author-Workplace-Name: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India Title: Inheritance and allelism of brown midrib trait introgressed in agronomically promising backgrounds in pearl millet (Pennisetum glaucum (L.) R. Br.) Abstract: The lignin content of forage crops significantly affects the livestock's forage intake and its dry matter digestibility. The brown midrib (bmr) trait is known for its association with a reduced lignin content and increased digestibility. Pearl millet inbreds having the bmr trait were derived from two populations ICMV bmr and WRajPop bmr via continued selfing up to 6-8 generations. The results from the crosses revealed the presence of the same single recessive gene that controlled the bmr in both the populations. The stable expression of the bmr gene in the new genetic background indicated that there was no effect of modifier genes. Four inbreds were identified with the stable expression of bmr (three in ICMV 155 and one in the WRajPop background) with a high biomass potential, which can serve as a bmr source in future pearl millet forage breeding programmes. Keywords: brown midrib introgression, crude protein, digestibility, forage yield, forage quality Journal: Czech Journal of Genetics and Plant Breeding Pages: 176-187 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/93/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/93/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202303-0007.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:3:id:93-2022-CJGPB