Template-Type: ReDIF-Article 1.0 Author-Name: Lijiao Gu Author-Workplace-Name: Institute of Forest Biotechnology, Forestry College, Hebei Agricultural University, Baoding, P.R. China Author-Name: Pengyun Chen Author-Workplace-Name: Beijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, Key Laboratory of Crop Heterosis Utilization, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing, P.R. China Author-Name: Shuxun Yu Title: The cytochrome P450 gene GhCYP94C1 is involved in drought stress in upland cotton (Gossypium hirsutum L.) Abstract: Cytochrome P450 proteins belong to one of the largest families of enzyme proteins in plants and play important roles in plant growth and development and the stress response. In our previous studies, a cytochrome P450 gene, GhCYP94C1 (cytochrome P450 94C1), was functionally characterized as a positive regulator of seed germination, main root elongation and early flowering. However, whether the gene has other potential functions remains to be further explored. In our study, expression analysis showed that GhCYP94C1 was highly expressed in roots and was suppressed by drought treatment. Endogenous silencing of GhCYP94C1 via virus-induced gene silencing (VIGS) increased drought resistance in cotton plants, which was accompanied by the upregulated expression of the abscisic acid (ABA) biosynthesis gene nine-cis-epoxycarotenoid dioxygenase 9 (GhNCED9) during drought stress. Our findings suggested that GhCYP94C1 may play an important role in drought resistance. Combined with previous research results, the present results provide a theoretical basis for future breeding of new cotton varieties with early maturation and drought resistance. Keywords: ABA signaling network, drought tolerance, expression analysis, qRT‒, PCR, VIGS assay Journal: Czech Journal of Genetics and Plant Breeding Pages: 189-195 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/108/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/108/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0001.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:108-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mala Ram Modi Author-Workplace-Name: Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, India Author-Name: Megha Katoch Author-Workplace-Name: Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, India Author-Name: Nisha Thakur Author-Workplace-Name: Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, India Author-Name: Manisha Gautam Author-Workplace-Name: Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, India Author-Name: Sunny Choudhary Author-Workplace-Name: Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, India Author-Name: Rakesh Kumar Chahota Title: Mapping of genomic regions associated with dwarfing and the determinate growth habit in horsegram (Macrotyloma uniflorum) Abstract: Horsegram (Macrotyloma uniflorum) - an important, self-pollinated food legume, however due to limited genomic and genetic resources the genetic improvement could not be achieved as compare to other major legumes. Our work aims at finding novel microsatellite markers and their use for the construction of a linkage map from 157 individuals of F9 recombinant inbred lines (RILs) of horsegram. The determinate growth habit and plant height are important traits for its suitability for different cropping systems. The genotypic data were generated by screening 2 395 molecular markers, of which 600 (25.05 %) polymorphic markers were selected. Two-hundred eighty-seven (287) markers were mapped on ten linkage groups (LGs) at a log of odds (LOD) of 3.5 straddling 796.76 cM with 2.78 cM of marker density. For the identification of the quantitative trait loci (QTLs), the phenotypic data recorded on the RILs for the plant height and growth habit were analysed using the statistical tools JoinMap®and Windows QTL cartographer, based on the composite interval mapping (CIM) technique. Across the ten linkage groups, we detected four QTLs (LOD ≥ 2.5) for four traits. All the traits were major QTLs as indicated by the percentage of phenotypic variance (PVE) (≥ 10%) that ranged from 13.5% to 40.3%, therefore, this is very important information which can be used in marker-assisted selection (MAS). The present genomic information generated in this orphan crop, thus, provides the base for genetic improvements by devising molecular breeding strategies. Keywords: determinate growth habit, dwarfing, horsegram, linkage map, Macrotyloma uniflorum Journal: Czech Journal of Genetics and Plant Breeding Pages: 196-204 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/119/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/119/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0002.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:119-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Jiang Su Author-Workplace-Name: Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, P.R. China Author-Name: Kanghua Xian Author-Workplace-Name: Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, P.R. China Author-Name: Chuanming Fu Author-Workplace-Name: Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, P.R. China Author-Name: Jinxiang He Author-Workplace-Name: Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, P.R. China Author-Name: Baojun Liu Author-Workplace-Name: Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, P.R. China Author-Name: Ningzhen Huang Title: Selection of suitable reference genes in Paulownia fortunei (Seem.) Hemsl. under different tissues and abiotic stresses for qPCR normalization Abstract: By choosing appropriate candidate reference genes (CRGs) and standardizing qPCR data, more accurate experimental data can be obtained. Herein, the expression stability of alpha-tubulin1 (TUA1), beta-tubulin (TUB), beta-tubulin 1 (TUB1), beta-tubulin 5 (TUB5), actin 1 (ACT1), actin 97 (ACT97), molecular chaperone dnaj (DNAJ), adenine phosphoribosyl transferase (APT), and histone H4 (HIS4) genes from Paulownia fortunei (Seem.) Hemsl. under different experimental conditions (different tissues, drought, salinity, Cd, and Cr treatments) was assessed with four statistical tools: RefFinder, BestKeeper, NormFinder, and geNorm. Notably, TUA1 and TUB5 were identified as CRGs for different tissues, ACT97 and TUB1 for drought treatment, ACT97 and APT for salinity treatment, TUB1 and ACT97 for Cd treatment, and DNAJ, TUB1 and TUB5 for Cr treatment. Furthermore, the results of "total" group, V4/V5 > 0.15 and V5/V6 < 0.15 revealed that the CRGs or gene combinations, which could meet all the test conditions, were not easy to identify. To further verify the reliability of CRGs, the expression levels of paulownia fortunei cellulose synthase A catalytic subunit2 (PfCesA2) and paulownia fortunei glutathione reductase (GR) genes were analysed. The expression patterns were different when the unstable CRGs were used for normalization compared to when the stable CRGs and combination were used for normalization. This study will lay a foundation for study on the expression levels of key genes from P. fortunei seedlings. Keywords: drought treatment, heavy metal, housekeeping genes, qRT-PCR, salinity treatment Journal: Czech Journal of Genetics and Plant Breeding Pages: 205-218 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/72/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/72/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0003.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:72-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Huali Yang Author-Workplace-Name: College of Agronomy, Hebei Agricultural University, North China Key Laboratory for Crop Germplasm Resources of China's Education Ministry, Baoding, Hebei, P.R. China Author-Name: Wenda Diao Author-Workplace-Name: College of Agronomy, Hebei Agricultural University, North China Key Laboratory for Crop Germplasm Resources of China's Education Ministry, Baoding, Hebei, P.R. China Author-Name: Xiaocui Yan Author-Workplace-Name: College of Agronomy, Hebei Agricultural University, North China Key Laboratory for Crop Germplasm Resources of China's Education Ministry, Baoding, Hebei, P.R. China Author-Name: Takele-Weldu Gebrewahid Author-Workplace-Name: College of Agriculture, Aksum University, Shire-Indaslassie, Tigray, Ethiopia Author-Name: Zaifeng Li Author-Name: Zhanjun Yao Title: Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials Abstract: Wheat leaf rust is a devastating disease worldwide. Identification of leaf rust resistance genes in seedlings and of genes for slow rusting are important in resistance breeding and for gene deployment to control the disease. A total of 108 wheat cultivars from the Beijing and Shandong province and a set of 36 differentials, mostly near-isogenic lines in the background of Thatcher with known leaf rust resistance genes, were tested with 20 Puccinia triticina pathotypes  (FHJS1, FGBQ,PGJQ, SHJT, FHGQ, PHTT1, FHGQ, FHGQ, PHJS, THSM, FHSQ, PHST, PRSQ, FNTQ, PHGM, KHGQ, PHTT2, TGTT, FHJS2, NHHT) at the seedling stage in the greenhouse. The cultivars and differentials were also planted in the field to test their slow rusting resistance using a mixture of races at Baoding, Hebei province and Zhoukou, Henan province, for two consecutive years. Ten leaf rust resistance genes, Lr1, 9, 10, 19, 20, 24, 26, 34, 37 and 46 were identified  in the 57 commercial wheat cultivars, either singly or in combination, using molecular markers. Combined, the results from gene postulation and marker detection showed that one or more of the genes Lr1, 3, 10, 14a, 14b, 26, 36, 39, 34 and 46, were present in 57 cultivars, and that no known resistance gene was present in the remaining 51 cultivars. The resistance gene Lr26 was present in 42 cultivars, and nine cultivars contained Lr1. Lr46 was present in 10 cultivars, as indicated by the presence of the closely linked marker csLV46G22. Seven genotypes were identified as possibly carrying the gene Lr39. Lr3 and 10 were found in six and four genotypes, respectively. The ggenes Lr14b and 34 were each present in three cultivars, while evidence for the presence of Lr14a and 36 was obtained in single genotypes. Finally, 12 cultivars showed slow rusting resistance at two locations in two crop seasons. The identification of leaf rust resistance genes in Chinese wheat cultivars will be helpful for gene deployment to control leaf rust. Keywords: gene postulation, Lr genes, molecular marker, slow rusting resistance, Puccinia triticina, Triticum aestivum Journal: Czech Journal of Genetics and Plant Breeding Pages: 219-234 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/9/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/9/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0004.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:9-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Li Wang Author-Workplace-Name: Kweichow Moutai Group, Renhuai, P.R.China Author-Name: Derang Ni Author-Workplace-Name: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, P.R. China Author-Name: Fan Yang Author-Workplace-Name: Kweichow Moutai Corporation Limited, Renhuai, P.R. China Author-Name: Lin Lin Author-Workplace-Name: Kweichow Moutai Corporation Limited, Renhuai, P.R. China Author-Name: Yubo Yang Author-Workplace-Name: Kweichow Moutai Corporation Limited, Renhuai, P.R. China Author-Name: Chongde Sun Author-Workplace-Name: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China Author-Name: Xingqian Ye Author-Workplace-Name: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, P.R. China Author-Name: Jinping Cao Author-Workplace-Name: The Rural Development Academy, Zhejiang University, Hangzhou, P.R. China Author-Name: Xiangli Kong Author-Workplace-Name: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China Title: Transcriptome profiling of Sorghum bicolor reveals cultivar-specific molecular signatures associated with starch and phenolic compounds biosyntheses and accumulation during sorghum grain development Abstract: Sorghum is an important crop, and starch and phenolic compounds are major and important components in the sorghum grain. However, the underlying critical genetic elements contributing to the rich portfolio of nutrients in sorghum grains are largely unknown. Transcriptomic methods were employed to characterize the expression patterns at five different grain developmental stages of Hongyingzi (an important brewing sorghum), and another two grain sorghums, Jinuoliang 1 and Hongliangfeng 1, for comparison. The uniquely expressed genes were identified at each developmental stage of Hongyingzi when compared with the other two sorghum cultivars. The co-regulated genes at different developmental stages and the regulatory network were determined; the determinant genes and single-nucleotide polymorphisms located at the promoters of these genes involved in starch and phenolic compounds biosynthetic pathways were also identified. These results will provide insights into the potential regulatory network and further contribute to the clarification of the key determinant genes involved in the biosyntheses of starch and phenolic compounds. Meanwhile, some new transcripts and genes were identified at five different developmental stages of grains of the three sorghum cultivars. Our work can provide impetus for further study of the genes responsible for the biosynthesis of starch and phenolic compounds in the sorghum grain, and pave a way for functional validation of a batch of potential genes and single-nucleotide polymorphisms proposed in current work. Keywords: biosynthesis, phenolic compounds, sorghum, starch, transcriptome profiling Journal: Czech Journal of Genetics and Plant Breeding Pages: 235-252 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/16/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/16/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0005.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:16-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Fernand Silvère Sohindji Author-Workplace-Name: Laboratory of Genetics, Biotechnology, and Seed Science (GBioS), Faculty of Agricultural Sciences, University of Abomey Calavi, Abomey-Calavi, Republic of Benin Author-Name: Florent J.-B. Quenum Author-Name: Nicodème V. Fassinou-Hotegni Author-Workplace-Name: Laboratory of Genetics, Biotechnology, and Seed Science (GBioS), Faculty of Agricultural Sciences, University of Abomey Calavi, Abomey-Calavi, Republic of Benin Author-Name: Adechina Adékounlé Oke Author-Workplace-Name: Laboratory of Genetics, Biotechnology, and Seed Science (GBioS), Faculty of Agricultural Sciences, University of Abomey Calavi, Abomey-Calavi, Republic of Benin Author-Name: Charlotte O. A. Adje Author-Workplace-Name: Laboratory of Genetics, Biotechnology, and Seed Science (GBioS), Faculty of Agricultural Sciences, University of Abomey Calavi, Abomey-Calavi, Republic of Benin Author-Name: Enoch G. Achigan-Dako Author-Workplace-Name: Laboratory of Genetics, Biotechnology, and Seed Science (GBioS), Faculty of Agricultural Sciences, University of Abomey Calavi, Abomey-Calavi, Republic of Benin Title: Crossing possibility for breeding promising orange-fleshed sweetpotato genotypes in Benin Abstract: Orange-fleshed sweetpotato (OFSP) is a sustainable and inexpensive source of vitamin A that contributes to food and nutritional security in developing countries. Its adoption is low due to unconformity of the variety with community food culture, limited cultivar selection and breeding studies. The current study aimed at breeding of promising OFSP genotypes in Benin using three parental lines. Two hundred and seventy-four seeds and one hundred and six genotypes were obtained through biparental crosses of Tio-joe (imported orange-fleshed variety), Vobodouaho (local white-fleshed variety) and ACAB220 (local orange-fleshed variety). The phenotypic diversity of genotypes was assessed using 10 leaf characters and storage root flesh colour through multiple correspondence analysis and hierarchical cluster analysis. The results indicated cross-compatibility between Tio-joe and ACAB220 when ACAB220 was used as female. Reciprocal cross-compatibility was found between ACAB220 and Vobodouaho. The genotypes could be separated into two main populations and classified into five cluster groups. Promising intermediate and pale orange genotypes were obtained indicating possibilities to incorporate beta-carotene into the white background of the Vobodouaho variety through further backcrossing. Keywords: beta-carotene, hand pollination, local varieties, open pollination, promising genotypes, white-fleshed sweetpotato Journal: Czech Journal of Genetics and Plant Breeding Pages: 253-262 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/104/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/104/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0006.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:104-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Gang Jing Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Name: Youhua Yao Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, China Author-Name: Likun An Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, China Author-Name: Yongmei Cui Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, China Author-Name: Yixiong Bai Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, China Author-Name: Xin Li Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Author-Workplace-Name: Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, China Author-Name: Xiaohua Yao Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Author-Name: Kunlun Wu Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, Qinghai, China Title: Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe disease Abstract: To explore the role of WRKY transcription factors (TFs) in the resistance process of Qingke (Hordeum vulgare L. var. nudum Hook. f.), leaves of the leaf stripe disease-resistant variety Kunlun 14 and the susceptible variety Z1141 were sequenced by transcriptome sequencing (RNA-seq). A differentially expressed gene HvnWKRY1 was identified, and its disease-resistance function was preliminarily analysed. The result showed that the open reading frame (ORF) of the gene was 1 062 bp and encoded 354 amino acids. It contained the conserved WRKY domain (273-351) and belonged to the WRKY protein family. The phylogenetic tree results showed that HvWRKY1 was most closely related to Hordeum vulgare L. The WRKY family of Qingke, barley, maize and rice were divided into categories I, II, and III, among which HvWRKY1 was located in group III. Results of the quantitative real-time fluorescence PCR (qRT-PCR) showed that the expression of HvWRKY1 was significantly (P < 0.01) higher in leaf stripe infected leaves of Kunlun 14 than that of Z1141. In Arabidopsis thaliana transformed with HvWRKY1, resistance to Botrytis cinerea was enhanced. The RNA-seq analysis showed there were 824 differentially expressed genes (DEGs). Data of the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment indicated, that a plant-pathogen interaction pathway was enriched. This study is expected to provide a theoretical basis for further studies of functioning of  the Qingke gene HvWRKY1 in resistance to the leaf stripe disease. Keywords: barley leaf stripe disease, HvWRKY1 gene, Qingke, RNA-seq, transgenic Journal: Czech Journal of Genetics and Plant Breeding Pages: 263-277 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/14/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/14/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202304-0007.txt Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:14-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Editorial Department Title: Index of Volume 59_Authors Index Journal: Czech Journal of Genetics and Plant Breeding Pages: I-IV Volume: 59 Issue: 4 Year: 2023 File-URL: http://cjgpb.agriculturejournals.cz/artkey/cjg-202304-0008_index-of-volume-59-authors-index.php File-Format: text/html Handle: RePEc:caa:jnlcjg:v:59:y:2023:i:4:id:cjg-202304-0008