Template-Type: ReDIF-Article 1.0 Author-Name: Sabri Erbaş Author-Name: Hasan Baydar Author-Workplace-Name: Isparta University of Applied Sciences, Faculty of Agricultural, Department of Fied Crops, Isparta, Turkey Author-Name: Halil Hatipoğlu Author-Workplace-Name: GAP Agricultural Research Institute, Şanliurfa, Turkey Author-Name: Hasan Koç Author-Workplace-Name: Bahri Dağdaş International Agricultural Reseach Institute, Konya, Turkey Author-Name: Metin Babaoğlu Author-Workplace-Name: Trakya Agricultural Research Institute, Edirne, Turkey Author-Name: Arzu Köse Author-Workplace-Name: Transitional Zone Agricultural Research Institute, Eskişehir, Turkey Title: Genotypic and environmental variability and stability of seed yield, oil content and fatty acids in high-oleic and high-linoleic safflower (Carthamus tinctorius L.) lines and cultivars Abstract: The present study was to determine the genotypic and environmental variability and stability in seed yield, oil content, oil yield, oleic and linoleic acid of 10 safflower lines derived from a cross of Dinçer 5-18-1 × Montola 2000 together with six cultivars under six environments at five locations. The effects of genotypes, environments and genotype × environment interactions were highly significant (P < 0.01) for seed yield and oil content. Averaged across all environments, the seed yield was lowest in the cultivar Olas (2 352 kg/ha), and highest in the line Bay-Er 5 (2 869 kg/ha). According to mean (xi) and regression coefficient (bi) values, the Bay-Er 16 was better adapted to unfavourable environmental conditions, whereas the Bay-Er 1, Bay-Er 5 and Bay-Er 14 were better adapted to favourable environmental conditions. The highest oil content across environments, over 35%, was recorded in the line Bay-Er 15 and the cultivars Olas and Linas. The best adaptability to the environments was observed in the cultivar Olas. The oleic acid content of genotypes increased and the linoleic acid contents decreased from the north to the south latitudes. The oil content of genotypes grown in Southeastern Anatolia was higher than in the other regions. Within the regions, seed yield and oil content was higher after autumn sowing than after spring sowing. Keywords: agronomic and quality characters, Carthamus tinctorius, oleic and linoleic acid, stability analysis Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-11 Volume: 60 Issue: 1 Year: 2024 DOI: 10.17221/113/2022-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/113/2022-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202401-0001.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:113-2022-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Revanasiddayya Author-Name: Jayaprakash Mohan Nidagundi Author-Workplace-Name: Department of Genetics and Plant breeding, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Bashasab Fakrudin Author-Workplace-Name: Department of Biotechnology and Crop Improvement, University of Horticultural Sciences, Bagalkot, Karnataka, India Author-Name: Prakash Kuchanur Author-Workplace-Name: Department of Genetics and Plant breeding, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Lingappa Neelagiri Yogeesh Author-Workplace-Name: Department of Genetics and Plant breeding, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Shivanand Hanchinal Author-Workplace-Name: Department of Agricultural Entomology, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Talagunda Chandrashekar Suma Author-Workplace-Name: Department of Crop Physiology, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Gururaj Sunkad Author-Workplace-Name: Dean (Post Graduate Studies), University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Bharamappanavara Muralidhara Author-Workplace-Name: Department of Genetics and Plant breeding, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Doddamani Maheshkumar Author-Workplace-Name: Department of Biotechnology and Crop Improvement, University of Horticultural Sciences, Bagalkot, Karnataka, India Author-Name: Channabasava Author-Workplace-Name: Department of Genetics and Plant breeding, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Patil Sudha Author-Workplace-Name: AICRP on Cotton, MARS, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Shivamurthy Rashmi Author-Workplace-Name: AICRP on Cotton, MARS, University of Agricultural Sciences, Raichur, Karnataka, India Author-Name: Virupapura Cholaraya Raghavendra Author-Workplace-Name: Department of Genetics and Plant breeding, University of Agricultural Sciences, Raichur, Karnataka, India Title: Genetic diversity among coloured cotton genotypes in relation to their fibre colour and ploidy level based on SSR markers Abstract: Genetic diversity is referred to as any variation at the phenotypic, DNA or genomic level of an individual, population or species. The appraisal of diversity is important to understand its pattern and evolutionary relationships between germplasms or genotypes, which will aid in sampling the genetic resources in a more systematic manner for conservation and crop improvement. The present study employed 50 simple sequence repeat (SSR) markers linked to the yield and fibre quality/colour traits for estimating the genetic diversity in 33 cotton genotypes of diploid and tetraploid species differing in fibre colour. The diversity analysis was performed in GenAlEx (Ver. 6.41) and Powermarker (Ver. 3.25) while DARwin (Ver. 6.0.21) software was used to establish the phylogenetic relationships following neighbour-joining (NJ) and unweighted pair group method with arithmetic (UPGMA) mean method. Markers generated 186 polymorphic loci as genotypic data with an average of 3.72 alleles and an average polymorphic information content (PIC) value of 0.59 per SSR locus. The NJ and UPGMA grouped 33 genotypes into three major clusters I, II and III consisting of 21 tetraploid Gossypium hirsutum, 10 G. arboreum coloured and 2 white cotton genotypes, respectively. In the PCA, the first two components (PC1 and PC2) explained 74.69% of the variation and the biplot plotted the 33 genotypes in three groups. The study established the diverse nature of 33 cotton genotypes based on their fibre colour and ploidy level. With confirmation of the prevalent genetic diversity, we suggest that hybridisation can be planned among diverse genotypes to unleash greater variation in the fibre colour or to derive superior cross combinations. Keywords: Gossypium hirsutum, neighbour-joining, PCA, polymorphism level, UPGMA Journal: Czech Journal of Genetics and Plant Breeding Pages: 12-24 Volume: 60 Issue: 1 Year: 2024 DOI: 10.17221/12/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/12/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202401-0002.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:12-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Yawo Mawunyo Nevame Adedze Author-Name: Xia Lu Author-Workplace-Name: Molecular Biology Laboratory of Suqian City GreenPort Modern Agriculture Research Institute, Suqian, Jiangsu Province, P.R. China Author-Name: Wenyi Fan Author-Workplace-Name: Molecular Biology Laboratory of Suqian City GreenPort Modern Agriculture Research Institute, Suqian, Jiangsu Province, P.R. China Author-Name: Wenting Zhang Author-Workplace-Name: Molecular Biology Laboratory of Suqian City GreenPort Modern Agriculture Research Institute, Suqian, Jiangsu Province, P.R. China Author-Name: Xue Yang Author-Workplace-Name: Molecular Biology Laboratory of Suqian City GreenPort Modern Agriculture Research Institute, Suqian, Jiangsu Province, P.R. China Author-Name: Zhijun Deng Author-Workplace-Name: Jiangsu GreenPort Modern Agriculture Development Company, Suqian, Jiangsu Province, P.R. China Author-Name: Md. Amirul Alam Author-Workplace-Name: Faculty of Sustainable Agriculture, Horticulture and Landscaping Program, University Malaysia Sabah, Sandakan, Sabah, Malaysia Author-Name: Guangli Xu Author-Workplace-Name: Jiangsu GreenPort Modern Agriculture Development Company, Suqian, Jiangsu Province, P.R. China Author-Name: Lihua Zhang Author-Workplace-Name: Suqian University, Suqian, Jiangsu Province, P.R. China Author-Name: Wenhu Li Author-Workplace-Name: Jiangsu GreenPort Modern Agriculture Development Company, Suqian, Jiangsu Province, P.R. China Title: Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis  (BSA-seq) in melon Abstract: Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F2 segregating population of melons generated from these two parents was inoculated by the PM pathogen. Based on bulk segregant analysis (BSA) using these 100 InDel markers, the powdery mildew resistance was associated with the genomic region LVpm12.1 on the melon chromosome 12. This region overlapped the previously described quantitative trait locus (QTL)-hotspot area carrying multiple PM-resistance QTLs. Moreover, conventional QTL mapping analysis was done, which located LVpm12.1 in the region between 22.72 and 23.34 Mb, where three highly polymorphic InDel markers MInDel89, MInDel92, and MInDel93 were detected. Therefore, these markers could be used to track this resistance locus in melon while the lines carrying this locus could be employed in PM melon resistance breeding programs after validation tests. Keywords: InDel marker, mapping, whole genome re-sequencing, resistance QTL Journal: Czech Journal of Genetics and Plant Breeding Pages: 25-33 Volume: 60 Issue: 1 Year: 2024 DOI: 10.17221/40/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/40/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202401-0003.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:40-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Martin Hádlík Author-Name: Miroslav Baránek Author-Workplace-Name: Mendeleum - Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: Ivana Flajšingerová Author-Workplace-Name: VINSELEKT MICHLOVSKÝ a.s., Rakvice, Czech Republic Author-Name: Kateřina Baránková Author-Workplace-Name: Mendeleum - Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Title: Verification of resistance loci pyramiding in popular interspecific grape varieties using SSR markers Abstract: Fungal pathogens Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni (downy mildew) and Erysiphe necator Schwein. (powdery mildew) represent the biggest threats for grape growers worldwide. Under suitable conditions, these pathogens can spread very quickly through vineyards and cause significant damage. The most ecological way to reduce the possibility of infection in vineyards is growing interspecific genotypes which are able to suppress these pathogens and stop the spread of infection. With this in mind, 9 international and 11 Czech grapevine genotypes were analysed in order to genotype the resistance loci present in them. As a tool a set of SSR markers linked to known loci of resistance to downy and powdery mildew was used. Namely, presence of four loci responsible for resistance to Plasmopara viticola (Rpv3, Rpv4, Rpv7, and Rpv10) and two for Erysiphe necator (Ren3 and Ren9) loci were analysed with respective SSR markers. By this way the degree of resistance gene pyramiding was newly assessed in all analysed cultivars and their perspectives in grapevine breeding are discussed. Keywords: downy mildew, Erysiphe necator, grapevine, Plasmopara viticola, powdery mildew, resistance Journal: Czech Journal of Genetics and Plant Breeding Pages: 34-41 Volume: 60 Issue: 1 Year: 2024 DOI: 10.17221/43/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/43/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202401-0004.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:43-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Melike Bakir Author-Name: Şehriban Demir Author-Workplace-Name: Department of Agricultural Biotechnology, Faculty of Agriculture, Erciyes University, Kayseri, Türkiye Author-Name: Cebrail Yildirim Author-Workplace-Name: Institute of Hemp Research, Yozgat Bozok University, Yozgat, Türkiye Author-Name: Abdullah Kahraman Author-Workplace-Name: Department of Field Crops, Faculty of Agriculture, Harran University, Sanliurfa, Türkiye Title: Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes Abstract: Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition, we tested the transferability of 86 SSR markers, including the 33 newly developed ones, to other legume species. Transferability rates of lentil SSR markers to other species varied between 13.76% (Vicia sativa L.) and 2.58% (Phaseolus vulgaris L. and Trifolium pratense L.). These new SSR markers could be used in further molecular breeding, population genetics, and genetic mapping studies in lentils and other legume species. Keywords: cross-transferability, genomic library, legumes, simple sequence repeats Journal: Czech Journal of Genetics and Plant Breeding Pages: 42-49 Volume: 60 Issue: 1 Year: 2024 DOI: 10.17221/57/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/57/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202401-0005.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:57-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Suzana Pavlović Author-Name: Jelena Damnjanović Author-Workplace-Name: Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia Author-Name: Zdenka Girek Author-Workplace-Name: Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia Author-Name: Lela Belić Author-Workplace-Name: Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia Author-Name: Milan Ugrinović Author-Workplace-Name: Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia Title: Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli Abstract: The influence of the developmental stage of zygotic embryos and the composition and pH of the Gamborg induction medium B5 on the initiation and development of somatic embryos was investigated. The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved. Somatic embryos appeared directly on the hypocotyls of the explants, without the callus stage. On zygotic embryos in the early cotyledonary phase, three times higher regeneration was achieved compared to larger embryos in the cotyledonary phase. The induction of somatic embryogenesis did not occur during the growth of explants on the medium containinig 2,4-dichlorophenoxyacetic acid, nor on zygotic embryos in the late cotyledonary phase. Random amplified polymorphic DNA analysis showed the genetic stability of somatic embryo-derived plants, which makes this newly established protocol suitable for the regeneration and propagation of desirable broccoli genotypes. Keywords: embryos germination, immature zygotic embryos, RAPD analysis Journal: Czech Journal of Genetics and Plant Breeding Pages: 50-54 Volume: 60 Issue: 1 Year: 2024 DOI: 10.17221/26/2023-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/26/2023-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202401-0006.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:26-2023-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Editorial Department Title: List of Reviewers 2023 Journal: Czech Journal of Genetics and Plant Breeding Number: 0 Volume: 60 Issue: 1 Year: 2024 File-URL: http://cjgpb.agriculturejournals.cz/artkey/cjg-202401-0007_list-of-reviewers-2023.php File-Format: text/html Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:cjg-202401-0007