Template-Type: ReDIF-Article 1.0 Author-Name: Hongyan Li Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Subcenter of National Hulless Barley Improvement, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, P.R. China Author-Name: Youhua Yao Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Subcenter of National Hulless Barley Improvement, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, P.R. China Author-Name: Likun An Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Subcenter of National Hulless Barley Improvement, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, P.R. China Author-Name: Xin Li Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Subcenter of National Hulless Barley Improvement, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, P.R. China Author-Name: Yongmei Cui Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Subcenter of National Hulless Barley Improvement, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, P.R. China Author-Name: Yixiong Bai Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Qinghai Subcenter of National Hulless Barley Improvement, Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining, Qinghai, P.R. China Author-Name: Xiaohua Yao Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Author-Name: Kunlun Wu Author-Workplace-Name: Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, P.R. China Title: Isolation and expression analysis of the HvnAnt2 gene in qingke barley (Hordeum vulgare L. var. nudum Hook. f.) varieties with different grain colours Abstract: To investigate the role of the HvnAnt2 gene in the formation of different qingke barley grain colours, HvnAnt2 was isolated from the leaves of the White 91-97-3 (white), Blue qingke (blue), Kunlun 17 (black), and Purple qingke (purple). The HvnAnt2 gene encodes a hydrophilic unstable protein consisting of 561 amino acids, without a transmembrane structure and without a signal peptide, with one bHLH-MYC_N and one HLH domain at amino acids 22-204 and 387-436, respectively. The HvnAnt2 of qingke barley was most closely related to Hordeum vulgare and most distantly related to Triticum monococcum; it was mainly related to light responsiveness and methyl jasmonate responsiveness. The Ant2 gene was highly expressed in Kunlun 17 and Purple qingke barley grains during the later stage of development. The protein interaction prediction showed that flavonoid 3'-monooxygenase, anthocyanin biosynthesis gene regulators, and key enzymes in folate metabolism interacted with the Ant2 protein. This study provides a reference for further analysis of the Ant2 gene in the anthocyanin synthesis pathways of qingke barley with different grain colours. Keywords: anthocyanin, bHLH, expression pattern, Tibetan hulless barley Journal: Czech Journal of Genetics and Plant Breeding Pages: 107-118 Volume: 60 Issue: 3 Year: 2024 DOI: 10.17221/7/2024-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/7/2024-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202403-0001.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:3:id:7-2024-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Hong-Wei Zhang Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Ling-Zhi Wang Author-Workplace-Name: Qiandongnan Miao and Dong Autonomous Prefecture Academy of Agricultural Sciences, Kaili, P.R.China Author-Name: Ying Xie Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Hao Liu-Gen Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Zhen-Zhen Wang Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Chong-Fen Yi Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Hui Guo Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Yu Gan Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Guan-Lun Xiang Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Yan Zhi-Qiang Author-Workplace-Name: Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Ze Song Author-Workplace-Name: Institute of Crop Germplasm Resources, Guizhou Academy of Agricultural Sciences, Guiyang, P.R.China Author-Workplace-Name: Key Laboratory of Crop Gene Resources and Germplasm Innovation in Karst Mountainous Areas, Ministry of Agriculture and Rural Affairs, Guiyang, P.R.China Author-Name: Zhan-Lie Yang Author-Workplace-Name: Institute of Crop Germplasm Resources, Guizhou Academy of Agricultural Sciences, Guiyang, P.R.China Title: QTL mapping for heading date and plant height using a RIL population in rice in different photoperiod environments Abstract: Heading date determines rice seasonal and regional adaptation, while plant height is an important trait related to rice lodging resistance. In this study, a recombinant inbred line population was used to detect quantitative trait loci (QTLs) for both traits in long-day (LD) and short-day (SD) environments. Three and two QTLs for heading date were detected in LD and SD environments, respectively. Notably, qHD8 and qHD10 were commonly detected in both environments. Five and four QTLs for plant height were identified in LD and SD environments, respectively. Among them, qPH3, qPH5 and qPH6 showed no pleiotropic effects on heading date and were detected in both environments. These three QTLs are considered to be the primary targets for improving rice plant height. Additionally, two genomic regions exhibited pleiotropic effects on both heading date and plant height. The alleles delayed the heading date while simultaneously increasing plant height. This study indicated that most QTLs for heading date are sensitive to photoperiod and have pleiotropic effects on plant height, thereby complicating their application in breeding programs. These findings provide useful information for the breeding of rice varieties with desired heading dates and plant heights. Keywords: gene-environment interaction, genetic mapping, Oryza sativa L., pleiotropic effect, quantitative trait loci Journal: Czech Journal of Genetics and Plant Breeding Pages: 119-125 Volume: 60 Issue: 3 Year: 2024 DOI: 10.17221/2/2024-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/2/2024-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202403-0002.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:3:id:2-2024-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Tasnim Khandaker Samiha Author-Workplace-Name: Department of Mathematics and Natural Sciences, BRAC University, Mohakhali, Dhaka, Bangladesh Author-Name: Md Ataur Rahman Author-Workplace-Name: Advanced Seed Research and Biotech Centre, ACI Limited, Dhaka, Bangladesh Author-Name: Saiful Islam Author-Workplace-Name: Advanced Seed Research and Biotech Centre, ACI Limited, Dhaka, Bangladesh Author-Workplace-Name: National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, P.R. China Author-Name: Nusrat Jalal Author-Workplace-Name: Department of Mathematics and Natural Sciences, BRAC University, Mohakhali, Dhaka, Bangladesh Author-Name: Aparna Islam Author-Workplace-Name: Department of Mathematics and Natural Sciences, BRAC University, Mohakhali, Dhaka, Bangladesh Author-Name: Abu Shamim Mohammad Nahiyan Title: Exploring potato diversity: A comprehensive genetic and phenotypic analysis of quantitative and qualitative traits Abstract: For sustainable breeding in potato, a better understanding of genetic diversity within germplasm banks for sustainable breeding is needed. This study comprehensively characterised the molecular and phenotypic traits of 62 potato accessions, including advanced clones and indigenous potato varieties from Advanced Chemical Industries Limited (ACI Ltd.), Bangladesh, and 8 varieties from the Bangladesh Agricultural Research Institute (BARI). By using 9 SSR markers and 13 morphological traits, including both quantitative and qualitative traits, we observed correlation coefficients ranging from -0.3 to 0.7 for 8 quantitative traits, and Pearson's chi-square (χ2 value) ranging from 24.3 to 135.4 for 5 qualitative characteristics. Molecular analyses identified 46 unique alleles, with 93.5% polymorphism. The markers STM0031 and STM1016 had the highest PIC value of 0.9. Genetic parameters for SSR markers included effective number of alleles per locus (Ne) = 5.6, unbiased expected heterozygosity (uh) = 0.8, diversity (h) = 0.8 and Shannon's information index (I) = 1.8. Jaccard's similarity coefficients ranged from 0.2 to 0.8, representing significant diversity. Cluster analysis, using unweighted pair-group method with arithmetic average (UPGMA), grouped the accessions into five clusters based on SSR profiles. An association was foud between the marker STM0031 and two traits: the number of tubers per hill and the content of reducing sugars in the tubers. This study provides information on genetic diversity and marker efficacy. It will guide future breeding programmes towards the development of high-yielding and industrially valuable potato varieties. Keywords: gene bank, genotype, high yield, industrial trait, SSR marker Journal: Czech Journal of Genetics and Plant Breeding Pages: 126-135 Volume: 60 Issue: 3 Year: 2024 DOI: 10.17221/10/2024-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/10/2024-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202403-0003.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:3:id:10-2024-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zhiqiang Yan Author-Workplace-Name: Guizhou Provincial Academy of Agricultural Sciences, Guiyang, P.R. China Author-Name: Ruyue Deng Author-Workplace-Name: Guizhou Provincial Academy of Agricultural Sciences, Guiyang, P.R. China Author-Name: Huihui Tang Author-Workplace-Name: Guizhou Provincial Academy of Agricultural Sciences, Guiyang, P.R. China Author-Name: Hongwei Zhang Author-Workplace-Name: Guizhou Provincial Academy of Agricultural Sciences, Guiyang, P.R. China Author-Name: Susong Zhu Author-Workplace-Name: Guizhou Provincial Academy of Agricultural Sciences, Guiyang, P.R. China Title: Molecular basis of differential sensitivity to MeJA in floret opening between indica and japonica rice Abstract: Methyl jasmonate (MeJA) accelerated floret opening in rice, crucial for addressing inconsistent floret opening in hybrid seed production of indica × japonica hybrids. However, indica and japonica rice exhibited notable differences in their sensitivity to MeJA's impact on floret opening, with indica responding faster. To clarify the molecular basis of MeJA-induced floret opening sensitivity differences between indica and japonica rice, after 1 h of MeJA spraying, transcriptomes and endogenous floret opening regulators were compared between indica and japonica rice, with histology differences observed. The results showed that MeJA quickly activated plant hormones in indica and japonica rice, but JAZ genes in JA signalling pathway upregulation were greater in indica rice. Alpha-linolenic acid, starch and sucrose metabolism pathways responded faster in indica rice. MeJA boosted endogenous JA synthesis more in indica rice, which had 5-6 middle-layer cells, more than japonica's 3-4 layers, resulting in thicker glumes. The metabolic pathways regulating floret opening, including plant hormone signal transduction, alpha-linolenic acid and starch and sucrose metabolism, along with endogenous JA and soluble sugars, could respond to exogenous MeJA. However, the more intense and rapid responses of these regulatory pathways and endogenous substances to MeJA in indica, resulted in the difference in sensitivity to MeJA between indica and japonica rice. Keywords: floret opening time, indica and japonica subspecies, MeJA, RNA-seq Journal: Czech Journal of Genetics and Plant Breeding Pages: 136-148 Volume: 60 Issue: 3 Year: 2024 DOI: 10.17221/43/2024-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/43/2024-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202403-0004.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:3:id:43-2024-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: İmren Çöken Tekin Author-Workplace-Name: Department of Field Crops, East Mediterranean Transitional Zone Agricultural Research of Institute, Kahramanmaras, Türkiye Author-Name: Ali Tekin Author-Workplace-Name: Department of Field Crops, East Mediterranean Transitional Zone Agricultural Research of Institute, Kahramanmaras, Türkiye Author-Name: Ziya Dumlupinar Author-Workplace-Name: Department of Agricultural Biotechnology, Faculty of Agriculture, Kahramanmaras Sutcu Imam University, Kahramanmaras, Türkiye Title: Evaluation of Levante × Karakilçik durum wheat advanced lines for yield related and quality traits using principal component biplot analysis Abstract: The Karakilçik landrace is known for its quality traits, especially protein content. However, its high plant height causes lodging and yield loss, which limits the production area. A Levante × Karakilçik population developed by crossing the local Karakilçik wheat with the commercial variety Levante in order to eliminate the disadvantages of Karakilçik and adapt it to wider production areas. In the study, yield, quality, and some physiological traits were investigated in the population. The experiment was conducted in an augmented experimental design in the cropping seasons of 2021-2022 and 2022-2023. A total of 36 genotypes from Levante × Karakilçik population, parents and 2 controls were tested. The tested genotypes varied significantly for all investigated traits (P < 0.01). Principal component biplot analysis explained 69.3% of the variations and relationships between the traits and genotypes. Among the investigated traits, genotypes LK26, LK19 and LK3 outperformed their parents in 5 traits (spike length (SL), number of spikelets per spike (SNS), number of grain numbers per spike (GNS), protein content (PC) and wet gluten content (G)), 4 traits (SNS, grain weight per spike (GWS), GNS and PC) and 3 traits (grain yield (GY), GNS and test weight (TW)), respectively. The genotype LK3 for yield and the genotypes LK19 and LK26 for quality traits could be recommended to growers and breeders. Keywords: Karaki, lçi, k landrace, PCA biplot, protein content, yield Journal: Czech Journal of Genetics and Plant Breeding Pages: 149-157 Volume: 60 Issue: 3 Year: 2024 DOI: 10.17221/18/2024-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/18/2024-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202403-0005.txt Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:3:id:18-2024-CJGPB