Template-Type: ReDIF-Article 1.0 Author-Name: Efi Sarri Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Anastasios Katsileros Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Sofia Migardou Author-Workplace-Name: Laboratory of Mycology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute (BPI), Athens, Greece Author-Name: Panagiotis Viliotis Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Ioannis Sidiropoulos Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Dimitris Sifnaios Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Pavlos Diamantis Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Nikolaos Sklavounos Author-Workplace-Name: Laboratory of Mycology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute (BPI), Athens, Greece Author-Name: Eleni M. Abraham Author-Workplace-Name: Faculty of Forestry and Natural Environment, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece Author-Name: Penelope J. Bebeli Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Author-Name: Nasya Tomlekova Author-Workplace-Name: Molecular Biology Laboratory, Department of Breeding, Maritsa Vegetable Crops Research Institute, Agricultural Academy, Plovdiv, Bulgaria Author-Name: Dimosthenis Kizis Author-Workplace-Name: Laboratory of Mycology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute (BPI), Athens, Greece Author-Name: Eleni Tani Author-Workplace-Name: Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, Greece Title: Evaluation of gamma-irradiated Pisum sativum germplasm for agronomic traits and tolerance to Didymella pinodes Abstract: Ascochyta blight, caused by a complex of pathogenic fungi including Didymella pinodes, Ascochyta pisi, and Phoma pinodella, is a major disease of field pea (Pisum sativum), causing severe losses through lesions on leaves, stems, and pods. Mutation breeding using gamma irradiation is a non-GMO strategy to induce genetic variation and accelerate the development of improved genotypes. In this study, the M2 generation of the forage pea cultivar Dodoni (Pisum sativum L. var. arvense), derived from M0 seeds irradiated with 100 Gy, was evaluated for tolerance to D. pinodes (CBS 251.47) using a detached-leaf assay under controlled greenhouse conditions. Disease progression was quantified via image-based analysis on the 3rd and 5th days post-infection, calculating diseased area and disease severity index. Extensive phenotypic evaluation was also conducted on 16 families in the greenhouse and 100 families under field conditions, using an augmented incomplete block design. Screening revealed several M2 families with significantly improved tolerance compared to non-irradiated controls. Among these, some individuals combined enhanced resistance with improved yield-related traits, such as higher pod number and biomass, while others exhibited reduced agronomic performance. These findings highlight the phenotypic diversity induced by gamma irradiation and demonstrate the potential to generate dual-purpose pea genotypes with both disease resistance and enhanced productivity, providing valuable material for future breeding of resilient cultivars. Keywords: Ascochyta blight, disease tolerance, field pea, mutagenesis, mutation breeding Journal: Czech Journal of Genetics and Plant Breeding Pages: 1-13 Volume: 62 Issue: 1 Year: 2026 DOI: 10.17221/84/2025-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/84/2025-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202601-0001.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:1:id:84-2025-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Pavlína Máchová Author-Name: Helena Cvrčková Author-Workplace-Name: Department of Forest Tree Species Biology and Breeding, Forestry and Game Research Institute, Strnady, Jíloviště, Czech Republic Author-Name: Olga Trčková Author-Workplace-Name: Department of Forest Tree Species Biology and Breeding, Forestry and Game Research Institute, Strnady, Jíloviště, Czech Republic Author-Name: Kateřina Vítová Author-Workplace-Name: Department of Forest Tree Species Biology and Breeding, Forestry and Game Research Institute, Strnady, Jíloviště, Czech Republic Author-Name: Johana Pechačová Author-Workplace-Name: Department of Forest Tree Species Biology and Breeding, Forestry and Game Research Institute, Strnady, Jíloviště, Czech Republic Author-Name: Václav Buriánek Author-Workplace-Name: Department of Forest Tree Species Biology and Breeding, Forestry and Game Research Institute, Strnady, Jíloviště, Czech Republic Author-Name: Petr Maděra Author-Workplace-Name: Department of Forest Botany, Dendrology and Geobiocoenology, Faculty of Forestry and Wood Technology, Mendel university in Brno, Brno, Czech Republic Author-Name: Kateřina Houšková Author-Workplace-Name: Department of Silviculture, Faculty of Forestry and Wood Technology, Mendel university in Brno, Brno, Czech Republic Author-Name: Tomáš Vyhnánek Author-Workplace-Name: Department of Plant Biology, Faculty of AgriSciences, Mendel university in Brno, Brno, Czech Republic Author-Name: Pavel Hanáček Author-Workplace-Name: Department of Plant Biology, Faculty of AgriSciences, Mendel university in Brno, Brno, Czech Republic Title: Microsatellite markers as a useful tool for species identification and assessment of genetic diversity of the Tilia species in the Czech Republic Abstract: Diagnostic morphological traits distinguishing Tilia cordata and T. platyphyllos are not always apparent. Precise species identification is crucial for conservation management and for establishing genetic resources of forest reproductive material. In this study, we employed fifteen microsatellite (SSR) markers to identify diagnostic loci for reliable species discrimination between T. cordata and T. platyphyllos species and to evaluate the genetic diversity. A total of 250 trees were sampled from eight natural autochthonous populations of T. cordata and T. platyphyllos in the Czech Republic, and additional ten individuals of T. tomentosa were included for comparison. Markers Tc8 and Tc918 were identified as diagnostic, exhibiting species-specific alleles for T. cordata and T. platyphyllos. Contrary to the previously published results, we obtained PCR amplicons at locus Tc918 with an allele size of 226 bp in T. cordata. An analysis of the fifteen SSR loci revealed a high level of genetic variability in both the T. cordata and T. platyphyllos populations. No clonally identical genotypes were detected across the eight study sites. The genetic differentiation (Fst) was higher between T. platyphyllos and T. tomentosa (0.316) than between T. platyphyllos and T. cordata (0.205). Bayesian clustering further revealed distinct genetic structures for T. cordata, T. platyphyllos, and T. tomentosa. Keywords: genetic variability, protection and reproduction of genetic resources, species discrimination, SSR markers, Tilia cordata, Tilia platyphyllos Journal: Czech Journal of Genetics and Plant Breeding Pages: 14-24 Volume: 62 Issue: 1 Year: 2026 DOI: 10.17221/102/2025-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/102/2025-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202601-0002.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:1:id:102-2025-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Xue Li Author-Workplace-Name: College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Workplace-Name: Technological Innovation Center for Biological Control of Crop Disease and Insect Pests of Hebei Province, Baoding, P.R. China Author-Name: Zhanhai Kang Author-Workplace-Name: College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Workplace-Name: Technological Innovation Center for Biological Control of Crop Disease and Insect Pests of Hebei Province, Baoding, P.R. China Author-Name: Jiaqi Zhang Author-Workplace-Name: College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Workplace-Name: Technological Innovation Center for Biological Control of Crop Disease and Insect Pests of Hebei Province, Baoding, P.R. China Author-Name: Sufen Dong Author-Workplace-Name: College of Information Science and Technology, Hebei Agricultural University, Baoding, Hebei, P.R.China Author-Workplace-Name: Technological Innovation Center for Biological Control of Crop Disease and Insect Pests of Hebei Province, Baoding, P.R. China Author-Name: Man Li Author-Workplace-Name: College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Author-Workplace-Name: Technological Innovation Center for Biological Control of Crop Disease and Insect Pests of Hebei Province, Baoding, P.R. China Author-Name: Xing Li Author-Workplace-Name: College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, P.R. China Title: The identification of wheat leaf rust resistance genes and their utilisation value in 42 wheat cultivars Abstract: Leaf rust is an important wheat disease that considerably reduces the wheat production in most wheat growing regions worldwide. This study aimed to identify leaf rust resistance genes in 42 wheat varieties to find genetic sources with the broadest spectrum of resistance against leaf rust pathotypes, to enable effective breeding for disease resistance. In this study, 42 wheat cultivars were inoculated with 18 pathotypes of Puccinia triticina Eriks. at the seedling stage to postulate the Lr genes in the cultivars. Resistance to leaf rust at the adult stage was then tested in field trials under natural infection during the 2019 to 2020 cropping seasons at Baoding, Hebei Province. Gene postulation together with molecular marker detection identified ten Lr genes (Lr1, Lr10, Lr14a, Lr26, Lr2a, Lr17, Lr20, Lr34, Lr37, and Lr46) among the 42 accessions.  Lr1 was present in 16 accessions, Lr14a in three accessions, Lr17 in five accessions, Lr2a in five accessions, Lr34 in one accession, Lr10 in two accessions, Lr37 in two accessions and Lr46 in 29 accessions. Additionally, 15 wheat accessions displayed adult-plant resistance or other unknown genes. These results suggest that a high level of leaf rust resistance can be achieved by combining known resistance genes and adult-plant resistance genes in wheat cultivars. Keywords: adult plant resistance, gene postulation, leaf rust, slow rust, Triticum Journal: Czech Journal of Genetics and Plant Breeding Pages: 25-35 Volume: 62 Issue: 1 Year: 2026 DOI: 10.17221/78/2025-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/78/2025-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202601-0003.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:1:id:78-2025-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Zuzana Rottová Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Tereza Anna Javůrková Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Petr Sedlák Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jiří Ptáček Author-Workplace-Name: Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Ltd., Havlíčkův Brod, Czech Republic Author-Name: Eloy Fernández-Cusimamani Author-Workplace-Name: Department of Crop Science and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Vladimíra Sedláková Title: Genetic diversity analysis of Solanum accessions from Czech collections of potato genetic resources using nuclear SSR markers Abstract: The genus Solanum comprises numerous wild and cultivated species that are important for potato breeding. This pilot-scale study aimed to evaluate the genetic diversity in 44 accessions from Solanum sect. Petota, comprising wild species, Andean landraces, and modern cultivars, obtained from the Potato Research Institute Havlíčkův Brod, Ltd. and the Department of Crop Sciences and Agroforestry at the Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague. Nuclear microsatellite markers (SSR, 29 loci) were applied via five multiplex PCR reactions and analysed using capillary electrophoresis. Binary data matrices were analysed using DARwin software to generate dendrograms reflecting allelic polymorphism. The SSR panel effectively differentiated cultivated accessions from wild types, consistent with the current taxonomy of the genus Solanum, with particularly clear clustering of Andean landraces and modern varieties. However, resolution among wild accessions was limited, likely due to their high genetic complexity and interspecific overlap. These results support the suitability of the SSR panel for analysing diversity in cultivated potatoes. while also highlighting the challenges in resolving wild Solanum taxa. This study contributes to germplasm characterisation and provides a molecular basis for future breeding programmes. Keywords: DNA polymorphism, germplasm conservation, microsatellite markers, multiplex PCR, wild potatoes Journal: Czech Journal of Genetics and Plant Breeding Pages: 36-48 Volume: 62 Issue: 1 Year: 2026 DOI: 10.17221/97/2025-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/97/2025-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202601-0004.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:1:id:97-2025-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Roberta Smimmo Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Grazia Buonfantino Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Clizia Villano Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Domenico Carputo Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Vincenzo D'Amelia Author-Workplace-Name: Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy Author-Name: Riccardo Aversano Title: Early activation of CIPK3 in the cold-tolerant potato Solanum commersonii Abstract: Solanum commersonii shows tolerance to low temperatures, a key target trait for potato breeding. Calcium-dependent protein kinases (CIPKs) play a significant role in plant defence response to several stresses, including cold. In this study, we observed the expression of ScCIPK1, ScCIPK3, ScCIPK23 and ScCIPK24 in S. commersonii exposed to 4 °C at multiple time intervals. Initial findings revealed that these genes were under-expressed after 10 and 30 minutes of cold stress, except ScCIPK3. Notably, after 24 hours, all genes displayed higher expression levels compared to the non-stressed controls. These findings highlight the role of ScCIPK3 in the early stages of cold response and indicate a coordinated regulatory mechanism across CIPKs that likely contributes to the cold stress tolerance observed in S. commersonii. In this work, we introduce a model to elucidate the signalling crosstalk under cold stress in S. commersonii, providing insights that could facilitate the development of cold-resistant potato cultivars. Keywords: abiotic stress, gene expression, plant adaptation, signal transduction, wild potato Journal: Czech Journal of Genetics and Plant Breeding Pages: 49-52 Volume: 62 Issue: 1 Year: 2026 DOI: 10.17221/51/2025-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/51/2025-CJGPB.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202601-0005.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:1:id:51-2025-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Editorial Department Title: List of Reviewers 2025 Journal: Czech Journal of Genetics and Plant Breeding Number: 0 Volume: 62 Issue: 1 Year: 2026 File-URL: http://cjgpb.agriculturejournals.cz/artkey/cjg-202601-0006_list-of-reviewers-2025.php File-Format: text/html Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:1:id:cjg-202601-0006