Template-Type: ReDIF-Article 1.0 Author-Name: Mollah Naimuzzaman Author-Workplace-Name: Department of Agroforestry and Environmental Science, Faculty of Agriculture, Sylhet Agricultural University, Sylhet, Bangladesh Author-Name: Faizur Rahman Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Md. Fayez Al-Hakim Khan Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Md Mahabub Hasan Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Farhana Tasmim Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Real Chandra Das Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Md. Rishad Khan Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Md. Mahdi Hasan Munna Author-Workplace-Name: Department of Agronomy and Haor AgricultureFaculty of Agriculture, Sylhet Agricultural University, Sylhet, Bangladesh Author-Name: Md Abdul Halim Author-Workplace-Name: Global Center for Environmental Remediation (GCER), College of Engineering Science and Environment, The University of Newcastle, Australia Author-Workplace-Name: Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, Bangladesh Author-Name: Kun Cho Author-Workplace-Name: Biopharmaceutical Mass Spectrometry Research Unit, Ochang Institute of Biological and Environmental Science, Korea Basic Science Institute, Ochang, Cheongju, Republic of Korea Author-Workplace-Name: Division of Bio-Analytical Science, University of Science and Technology (UST), Daejeon, Republic of Korea Author-Name: Md. Omar Sharif Author-Workplace-Name: Department of Agroforestry and Environmental Science, Faculty of Agriculture, Sylhet Agricultural University, Sylhet, Bangladesh Author-Name: Swapan Kumar Roy Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Farjana Sultana Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Author-Name: Fahim Ahmed Author-Workplace-Name: College of Agricultural Sciences, IUBAT-International University of Business Agriculture and Technology, Dhaka, Bangladesh Title: Evaluation of Tomato yellow leaf curl virus (TYLCV) resistance and yield performance of different genotypes of tomato Abstract: Since Tomato yellow leaf curl virus (TYLCV) causes severe losses in tomato crops worldwide the search for genetic resources for breeding high-yielding, disease-resistant varieties remains challenging. We tested 25 tomato genotypes under field conditions to identify high-yielding disease-resistant genotypes. We identified several promising genotypes using advanced statistical methods, particularly genotype G13 which had a high yield of 8.8 kg per plant with 8.7 fruits per cluster and showed a low disease severity under high natural infection pressure. The genotypes G2, G3, G10, G11 showed moderate resistance, with disease severity ranging from 12.5% to 19.4%. The highest-yielding genotypes also had higher numbers of branches and fruit clusters. The high heritability of these traits indicates their potential usefulness as selection criteria in breeding programmes. The identified genotypes might provide useful genetic resources for developing high-yielding tomato varieties suitable for cultivation in TYLCV-prone areas. This work bridges the critical gap between disease resistance and yield potential, giving farmers actionable options to protect their harvests. Keywords: disease severity, genetic variability, heritability, principal component analysis, whitefly (Bemisia tabaci) Journal: Czech Journal of Genetics and Plant Breeding Pages: 201-212 Volume: 62 Issue: 4 Year: 2026 DOI: 10.17221/54/2026-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/54/2026-CJGPB.html File-Format: text/html File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/54/2026-CJGPB.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202604-0001.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:4:id:54-2026-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Prashant Vasisth Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Mohit Sharma Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Omkar Maharudra Limbalkar Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Workplace-Name: ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India Author-Name: Gokulan Dhanasekaran Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Mohan Lal Meena Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Priyanka Jain Author-Workplace-Name: Division of Agricultural Bioinformatics, Indian Council of Agricultural Research -Indian Agricultural Statistics Research Institute, New Delhi, India Author-Name: Bhaskar Chandra Sahoo Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Sourav Kumar Moharana Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Mir Asif Iquebal Author-Workplace-Name: Division of Agricultural Bioinformatics, Indian Council of Agricultural Research -Indian Agricultural Statistics Research Institute, New Delhi, India Author-Name: Kiran B. Gaikwad Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Author-Name: Anshul Watts Author-Workplace-Name: ICAR-National Institute for Plant Biotechnology, New Delhi, India Author-Name: Naveen Singh Author-Workplace-Name: Division of Genetics, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute, New Delhi, India Title: Deciphering the genetic basis of heterosis for yield and its component traits using Brassica carinata-derived B. juncea alien introgression lines Abstract: The genetic basis of heterosis, which is widely exploited to increase crop yields, remains incompletely understood. Introgression lines created through interspecific hybridisation have been found to be useful for understanding the genetic basis of heterosis for economically important traits. Therefore, we used B. carinata-derived B. juncea introgression lines (ILs) to study the genetic basis of heterosis and identify genes associated with heterosis in elite hybrids. To assess the contribution of introgressed genomic segments in both homozygous and heterozy-gous states, ILs, introgression line hybrids (ILHs), and the B. juncea recipient parents were com-pared. High mid-parent heterosis in the hybrids for yield-related traits highlights the heterotic potential of the ILs. Comparisons among ILs, B. juncea parents, and ILHs showed that most ILHs outperformed both parents in seed yield and its component traits. Candidate genes such as ARR1, ARF5, TCP22, and BKK1 for seed weight; SASP and SBT1.8 for secondary branches and total siliquae; ATKT2, KASIII, UGT73B3, UGT72E1, and SESA1 for oil content; and FLA3 for siliqua length displayed a heterozygote advantage in ILHs. Therefore, the observed pheno-typic overdominant effect and heterozygote advantage at key candidate genes indicate that overdominance or pseudo-overdominance is a primary genetic basis of yield heterosis in Brassica. Keywords: Brassica juncea, genetic hypothesis, heterosis, interspecific hybridization, B. carinata derived lines Journal: Czech Journal of Genetics and Plant Breeding Pages: 213-226 Volume: 62 Issue: 4 Year: 2026 DOI: 10.17221/74/2026-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/74/2026-CJGPB.html File-Format: text/html File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/74/2026-CJGPB.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202604-0002.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:4:id:74-2026-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lili Fu Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Zhaoyang Hu Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Yu Zhang Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Yutong Liu Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Zhangqi Wu Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Yuelong Zhou Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Shiqiang Liu Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Hailin Xia Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Author-Workplace-Name: Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, P.R. China Author-Name: Yong Zhou Author-Workplace-Name: College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China Title: Identification and functional analysis of CsZIP9, a ZIP transporter involved in cadmium transport in cucumber (Cucumis sativus L.) Abstract: Agricultural soil contamination with cadmium (Cd) has become an increasingly serious global issue, and cucumber (Cucumis sativus L.) is highly sensitive to Cd stress. Zinc/iron-regulated transporter-like proteins (ZIPs) are involved in the uptake and translocation of divalent cations. However, the functional roles of cucumber ZIP members in Cd transport remain poorly understood. In this study, we cloned and characterised CsZIP9, a ZIP gene from cucumber. Sequence analysis revealed that CsZIP9 contains eight transmembrane domains and shares high similarity with known Cd‑transporting ZIPs. Expression analysis showed that CsZIP9 exhibits leaf‑preferential expression and is dynamically regulated by Cd stress. Heterologous expression of CsZIP9 in the Cd‑sensitive yeast mutant Δycf1 significantly increased Cd sensitivity, indicating its Cd transport activity. Moreover, overexpression of CsZIP9 in Arabidopsis enhanced Cd tolerance, as evidenced by reduced root growth inhibition under Cd treatment, although no direct measurements of Cd accumulation were conducted to support this conclusion. Taken together, these results indicate that CsZIP9 encodes a functional cadmium transporter, offering a promising candidate gene for enhancing Cd tolerance in cucumber Keywords: cadmium stress, expression analysis, transgenic Arabidopsis, zinc/iron-regulated transporter-like protein (ZIP) Journal: Czech Journal of Genetics and Plant Breeding Pages: 227-236 Volume: 62 Issue: 4 Year: 2026 DOI: 10.17221/81/2026-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/81/2026-CJGPB.html File-Format: text/html File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/81/2026-CJGPB.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202604-0003.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:4:id:81-2026-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Lirong Zhao Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Qiaofeng Xie Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Ziyue Hong Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Tao Chen Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Zhaoxin Hu Author-Workplace-Name: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, USA Author-Name: Shengwu Hu Author-Workplace-Name: College of Agronomy, Northwest A&F University, Yangling, Shaanxi, P.R. China Title: Development and characterisation of Ogu CMS root and leaf mustard Abstract: Mustard (Brassica juncea) is an important oilseed and vegetable crop in China with high genetic diversity. Among its various forms, root and leaf mustard are two important vegetable forms. Cytoplasmic male sterility (CMS) provides an important strategy for exploiting heterosis in mustard. Ogu CMS is a widely utilised sterility type in Brassica species; however, studies on this type of male-sterile material in root and leaf mustard remain limited. To address this gap, this study was designed to (i) introgress the Ogu CMS cytoplasm from B. napus into leaf and root mustard via backcrossing, (ii) determine the cytological stage and features of anther abortion in the newly developed CMS lines, and (iii) identify candidate genes and molecular pathways associated with male sterility through comparative transcriptome analysis. We successfully developed three leaf and one root mustard Ogu CMS lines and their maintainers. Both CMS and maintainer lines had 2n = 36 chromosomes. Molecular typing confirmed the Ogu CMS type in mustard CMS lines and the Cam type in their maintainers. CMS lines showed shorter filaments, pale yellow-white non-dehiscent anthers, and no pollen. Cytological observation revealed that pollen abortion in the CMS line initiated at the tetrad stage, characterised by the tapetal enlargement and microspore compression. Transcriptome analysis identified 7 595 differentially expressed genes (DEGs) between the CMS line and its maintainer, including 3 952 upregulated and 3 643 downregulated genes. Genes involved in lipid, fatty acid, and carbohydrate metabolism were predominantly downregulated, whereas those associated with mitochondrial organisation and reactive oxygen species responses were upregulated. Among the DEGs, we identified 513 transcription factors and 107 kinase genes. Integrating cytological and transcriptome data, we propose a putative abortion mechanism for this CMS system. Our findings not only provide novel male-sterile resources, including the first Ogu CMS line in root mustard, but also establish a theoretical foundation for heterosis breeding in mustard. Keywords: Brassica juncea, cytoplasmic male sterility, pollen abortion, tapetum development, transcriptome analysis Journal: Czech Journal of Genetics and Plant Breeding Pages: 237-249 Volume: 62 Issue: 4 Year: 2026 DOI: 10.17221/87/2026-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/87/2026-CJGPB.html File-Format: text/html File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/87/2026-CJGPB.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202604-0004.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:4:id:87-2026-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Mitali Tiwari Author-Workplace-Name: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India Author-Name: Anshuman Singh Author-Workplace-Name: ICAR-Indian Agricultural Statistics Research Institute, Pusa, New Delhi, India Author-Name: Shivam Yadav Author-Workplace-Name: G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India Author-Name: Meenakshi Arya Author-Workplace-Name: ICAR-National Research Institute for Integrated Pest Management, New Delhi, India Author-Name: Amit Kumar Author-Workplace-Name: Jawahar Lal Nehru Krishi Vishwavidyalaye, Jabalpur, Madhya Pradesh, India Author-Name: Priyanshi Garg Author-Workplace-Name: Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India Author-Name: Bhavana Tiwari Author-Workplace-Name: Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India Author-Name: Rakesh Choudhary Author-Workplace-Name: Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India Author-Name: Rumana Khan Author-Workplace-Name: Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India Author-Name: Ashutosh Singh Author-Workplace-Name: Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India Author-Name: Sushil Kumar Chaturvedi Author-Workplace-Name: Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India Title: Identification of high-yielding and stable resistance donors for yellow mosaic disease in blackgram (Vigna mungo L. Hepper) Abstract: Yellow mosaic disease (YMD) in blackgram (Vigna mungo L. Hepper) is caused by whitefly-transmitted begomoviruses, particularly Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV), which cause substantial economic losses across South and Southeast Asia. Exploitation of host plant resistance to develop resistant cultivars remains the most effective, economically feasible and sustainable management strategy. In northern India, MYMIV is a major viral disease affecting blackgram crop. The investigations were carried out to identify potential donors carrying genes imparting resistance against MYMIV-associated YMD in blackgram through large-scale field-based phenotyping using a standardised 1-9 disease rating scale. A diverse panel of 216 blackgram genotypes comprising released varieties, advanced breeding and experimental lines were evaluated under natural epiphytotic conditions at a MYMIV hotspot (Jhansi, India) during Kharif 2023 and Kharif 2024. Disease severity was recorded at the pod-filling stage, and the percent disease index (PDI) was calculated to classify genotypes into various disease reaction levels. Out of 216 genotypes, 51 genotypes were classified as resistant (PDI < 5%), 81 as moderately resistant (PDI = 5-10%), 58 as moderately susceptible, 25 as susceptible and only one accession as highly susceptible. Presence of the MYMIV-specific amplicon in plants exhibiting YMD symptoms confirmed MYMIV infection at the molecular level and complemented the PDI-based resistance classification. A highly significant negative correlation (r = -0.88***) was observed between PDI and SYPP, indicating that MYMIV-associated YMD severity accounts for approximately 77% of yield variation. Several released varieties, such as IC-236718, IPU-2K-99-221, KU-302 and elite breeding lines RLBU-L1-33 and RLBU-L2-15, showed stable resistance across both Kharif 2023/24 seasons. These genotypes may represent useful allelic diversity for MYMIV-associated YMD resistance and can serve as potential donors in future breeding programmes aimed at developing high-yielding, YMD-resistant blackgram varieties. Keywords: begomovirus, disease resistance, donor identification, field phenotyping, Mungbean yellow mosaic India virus (MYMIV), urdbean Journal: Czech Journal of Genetics and Plant Breeding Pages: 250-263 Volume: 62 Issue: 4 Year: 2026 DOI: 10.17221/41/2026-CJGPB File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/41/2026-CJGPB.html File-Format: text/html File-URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/41/2026-CJGPB.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjg-202604-0005.txt Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:4:id:41-2026-CJGPB Template-Type: ReDIF-Article 1.0 Author-Name: Editorial Department Title: INDEX OF VOLUME 62 Journal: Czech Journal of Genetics and Plant Breeding Pages: I-V Volume: 62 Issue: 4 Year: 2026 File-URL: http://cjgpb.agriculturejournals.cz/artkey/cjg-202604-0006_index-of-volume-62.php File-Format: text/html File-URL: http://cjgpb.agriculturejournals.cz/pdfs/cjg/2026/04/06.pdf File-Format: Application/pdf Handle: RePEc:caa:jnlcjg:v:62:y:2026:i:4:id:cjg-202604-0006